Microwave Acid Digestion Method Note Compendium€¦ · Compendium October 1, 2019. Contents...

MARS 6Microwave Acid Digestion

Method Note Compendium

October 1, 2019

Contents

Agriculture

Alfalfa 11

Boric Acid HF Neutralization 12

Cannabis (Plant) 13

Coffee Beans 14

Cotton 15

Feed Grain 16

Fertilizer - AOAC Method 2017.02 17

Fertilizer -AOAC Method 2006.03 18

Geranium 19

Impatiens 20

Leather 21

Palm Leaves 22

Plant Tissue 23

Soybean 24

Soybean Meal 25

Spinach Leaves 26

Straw 27

Tea Extract 28

Tea Tannin 29

Tobacco 30

Wheat 31

Wood 32

Wood Pulp 33

Wool 34

Yeast 35

Clinical & Biological

Animal Tissue (Dry) 37

Animal Tissue (Wet) 38

Beef Bone 39

Blood (Human) 40

Bovine Blood 42

Bovine Liver (Wet) 43

Brain Tissue (Dog) 44

Dog Feces (Dry) 45

Fingernails 46

Hair 47

Lobster Hepatopancreas (Tort-CRM) 48

Rat Kidney 49

Urine - Human 50

Consumer Products

Candle Fragrance in iPrep 53

Cosmetics (Liquid Make-Up) 54

Cotton 55

Deodorant (Powder) 56

Eye Shadow 57

Foam (Mattress) in iPrep 58

Sanitizers (Hand) 59

Shampoo (Dandruff) 60

Soaps (Lotion and Foam) 61

Sunscreen in iPrep 62

Suppository (Capsule) 63

Toothpaste 64

Vaseline 65

Wax (Candle) 66

Environmental & Regulatory

Fly Ash in iPrep 71

Limestone 73

NPDES (Waste Water) 74

Pine Needles 75

RoHS (For Pb, Hg, and Cd Analysis) 76

Sediment (BCCS - 1 CRM) (Leach) 77

Sediment (Buffalo River) (Leach) 78

Sludge (Industrial) 79

Soil (Montana - CRM) (Leach) 80

TCLP Extract 81

US EPA 3015 (Aqueous Samples) 82

US EPA 3015a (Aqueous Sample) 83

US EPA 3051 (Solid Sample) 84

US EPA 3051a (Solid Sample) 85

US EPA 3052 (Sludge, Soil, Sediment or Oil) 86

USP 232/233 (Pharmaceuticals) 87

Waste Activated Sludge 88

Water (For Analysis of Phosphorous) 89

Wood 90

Food Testing

Alum 92

Apple 93

Asparagus 94

Aspartame in iPrep 95

Bacon (Cooked) 96

Beef (Ground) 97

Beef Bouillon 98

Beer (Light) 99

Blueberry 100

Broccoli 101

Caramel Color 102

Carrot 103

Celery 104

Cheese (Cracker) 105

Cheese (Powder) 106

Cheese (Processed) 107

Cherry 108

Chewing Gum 109

Chicken (Boneless) 110

Chili Candy (Mexican) 111

Chili Powder in iPrep 112

Chips (Potato) 113

Citrus Leaves 114

Clam Puree 115

Cocoa 116

Coconut 117

Coffee Beans 118

Cucumber 119

Dog Food 120

Fish Tissue 121

Flounder 122

Flour 123

Food 124

Food Coloring (Liquid) 125

Fruit Juice 126

Gelatin 127

Granola Bar 128

Grape 129

Grapefruit 130

Gummy Bear 131

Ham 132

Infant Cereal (Rice Grain) 133

Infant Formula (Liquid) in iPrep 134

Infant Formula (Liquid) 135

Infant Formula (Powder) in iPrep 136

Infant Formula (Powder) 137

Kidney Bean 138

Kielbasa Sausage 139

Maple Syrup 140

Mayonnaise 141

Meal Replacement Shake (Chocolate) 142

Milk (Whole - Liquid) 143

Milk (Powder) 144

Nutritional Drink (Adult) 145

Olive Oil 146

Orange Juice 147

Oyster Puree 148

Peanut Butter 149

Pear 150

Pepper (Bell, Chili, Etc.) 151

Pistachio Nuts 152

Pizza (Frozen, Pepperoni) 153

Plum 154

Pork (Ground) 155

Potato 156

Potato Chips 157

Pretzel (Salted) 158

Protein Bar 159

Raspberry 160

Rice 161

Safflower Oil 162

Salad Dressing (Ranch) 163

Salami 164

Sausage 165

Soda (Diet) 166

Sodium Free - Seasoning 167

Soybean 168

Spinach 169

Spinach Leaves 170

Spinach Pasta 171

Strawberry 172

Sugar (Granulated) 173

Tea (Chai) in iPrep 174

Tea Leaves 175

Tomato Leaves 176

Tomato Paste (70% Moisture) 177

Tomato Soup 178

Walnut 179

Watermelon 180

Wheat Crackers 181

Whey 182

Whey (Powder) 183

Wine 184

Yogurt (Plain) 185

Geoscience & Mining

Alpha - Alumina in iPrep 187

Alumina Beads (with Silver Substrate) in iPrep 188

Alumina Powder in iPrep 190

Aluminum Nitride 191

Aluminum Oxide 192

Aluminum Oxide (H3PO4) in iPrep 193

Asbestos 194

Automotive Catalyst 195

Barium Titanate (BaTiO3) 196

Bastnaesite Ore 197

Bauxite 199

Bismuth Aluminate Mixture 201

Bismuth Yttrium Iron Oxide in iPrep 202

Boiler Scale (80-90% Fe) 204

Boron Carbide (B4C) in iPrep 206

Carbon 207

Cement 208

Ceramic (Fused Silica) 209

Ceramics 210

Char - Sulfuric Acid 211

Chromium Carbide 212

Chromium Oxide (Cr2O3) in iPrep 213

Clay 214

Coal in iPrep 215

Coal 216

Coal Ash 217

Cryolite / Electrolytic Bath 218

Feldspar in iPrep 220

Ferric Oxide in iPrep 222

Furnace Slag 223

Gadolinium Zirconium Oxide in iPrep 224

Gas Atomized Powder in iPrep 226

Glass Fiber 227

Hafnium Oxide 228

Ilmenite (FeTiO3) 229

Iron Ore 230

Kaolin 231

Kerosene 232

Lead Sulfide (PbS) 233

Lithium Nickel Cobalt Manganese Oxide 234

Magnesium Oxide (MgO 40-54%) 235

Manganous Oxide (MnO) 236

Mica in iPrep 238

Mill Tailings 239

Mineral Ore 241

Molybdenum Disulfide (MoS2) 243

Nickel (II) Hydroxide 245

Niobium Pentoxide 246

Petroleum Coke 247

Phosphate (21%) 249

Phosphate Rock 250

Pollucite Ore in iPrep 251

Pyrophyllite (AlSi2)5(OH) 253

Quartz Powder 254

Rock (Pulverized) 255

Rock, High Sulfide in iPrep 256

Rutile Ore 258

Sand 259

Shale in iPrep 260

Silica Sand 261

Silica Sand in iPrep 262

Silicon Dioxide 263

Silicon Nitride in iPrep 264

Silicon Wafer 265

Slag - Furnace 266

Sodium Antimonate in iPrep 267

Spodumene (Lithium Feldspar) 268

Sulfide Bulk Concentrate in iPrep 270

Tantalum Pentoxide (Ta2O5) in iPrep 272

Titanium Dioxide 273

Titanium Slag Ore in iPrep 274

Tungsten Carbide 275

Tungsten Ore (50% W; 10% Fe; 2% Mn) 276

Tungsten Oxide 277

Vermiculite 278

Wollastonite (Calcium Silicate) in iPrep 279

Zeolite 280

Zinc Oxide (ZnO) 281

Zinc Oxide (ZnO) in iPrep 282

Zircon in iPrep 283

Industrial Hygiene

Asbestos 285

Filter (MCE) 286

Filter Paper - 37 mm Mixed Cellulose Ester 287

Filter Paper - 47 mm Polycarbonate 288

Filter Paper - Cellulose 289

Ghost Wipe 290

Paint Chips (For Pb Analysis) 291

Quartz Fiber Filters 292

Toray Filter in iPrep 293

Toray Filter (Anion) 294

Toray Filter (Cation) 295

Materials Science

Acrylic Tape in iPrep 297

Bismuth Aluminate Mixture 298

Bismuth Yttrium Iron Oxide in iPrep 299

Ceramic Powder 301

Ceramic Powder in iPrep 302

Circuit Board (Cryo - Ground) 303

Ferric Oxide in iPrep 304

Gadolinium Zirconium Oxide in iPrep 305

Gas Atomized Powder in iPrep 307

Glass (Ground) 308

Graphite Composite - Cyanate Ester Resin 309

Graphite Composite - Epoxy Resin 310

Graphite Composite - PEEK Resin 311

Graphite Fiber - Cyanate Siloxane Resin 312

Graphite Fiber - Epoxy Resin (Fiber Content) 313

Pollucite Ore in iPrep 314

Siloxanes 316

Metals & Alloys

Alpha - Alumina in iPrep 318

Aluminum (Metal) 319

Aluminum Alloy 320

Aluminum Alloy (20% SiC) 321

Aluminum Alloy (BCS 182) 322

Aluminum Oxide 323

Aluminum Oxide (H3PO4) in iPrep 324

Boron 325

Boron Carbide (B4C) in iPrep 326

Carbonyl Iron in iPrep 327

Cobalt - Chromium Alloy (Co-Cr) 328

Copper (Metal) 330

Copper Ore 331

Gallium 332

Iron - Chromium Alloy 333

Iron - Chromium Alloy in iPrep 335

Iron - Lignosulfonate 336

Iron - Manganese Alloy 337

Iron - Silicon Alloy 338

Lead Sulfide (PbS) 339

Lithium Nickel Cobalt Manganese Oxide 340

Magnesium Oxide (MgO 40-54%) 341

Manganous Oxide (MnO) 342

Metal Alloy - FeCr 344

Metal Alloy - NiCr 345

Metal Alloy (Ag, Au, Cu) in iPrep 346

Metal Alloy (Ag, Co, Cu) in iPrep 347

Metal Alloy (Ag, Cu, Sn) in iPrep 348

Metal Ore - Precious 349

Metals Alloy (Co-Cr) 351

Nickel (II) Hydroxide 353

Nickel (Metal) 354

Niobium - Titanium Alloy 355

Phosphate (21%) 356

Platinum Metal 357

Platinum Ruthenium in iPrep 358

Rhodium Sponge in iPrep 359

Selenium Alloy 360

Sn - Ag Solder 361

Stainless and Low Carbon Steel in iPrep 362

Stainless Steel 363

Steel (Stainless) 364

Strontium Ferrite in iPrep 365

Titanium Alloy 366

Titanium Dioxide 367

Tungsten Ore (50% W; 10% Fe; 2% Mn) 368

Zinc Oxide (ZnO) 369

Zinc Oxide (ZnO) in iPrep 370

Zircon Alloy 371

Nutraceuticals

Biotin 373

Cannabis (Plant) 374

Chili Powder in iPrep 375

Citrus Leaves 376

Ginkgo (Ground) 377

Ginkgo (Whole Pill) 378

Infant Formula (Liquid) in iPrep 379

Infant Formula (Liquid) 380

Infant Formula (Powder) in iPrep 381

Infant Formula (Powder) 382

Kelp 383

Milk (Whole - Liquid) 384

Milk (Powder) 385

Plant Tissue 386

Wheat 388

Whey (Powder) 389

Organic Chemicals

Di-(2-ethylhexyl)phosphoric Acid in iPrep 391

Diazo Sulfonyl Chloride 392

Food Coloring (Erythrosine Based) 393

Methylcellulose (Hydroxypropyl) 394

Monoethanolamine 395

Photoresist 396

Polygluteramide 397

Paints & Coatings

Paint (Latex Based Liquid) 399

Paint Chips (For Pb Analysis) 400

Photoresist 401

Polyurethane 402

Polyurethane in iPrep 403

Toner (Ink) in iPrep 404

Toner (Powder) in iPrep 405

Paper & Pulp

Filter Paper - 37 mm Mixed Cellulose Ester 407

Filter Paper - 47 mm Polycarbonate 408

Filter Paper - Cellulose 409

Paper Pulp 410

Personal Care

Cosmetics (Liquid Make-Up) 413

Eye Shadow 414

Shampoo (Dandruff) 415

Pharmaceutical & Biotech

Allergy Pill (Ground) 417

Allergy Pill (Whole Pill) 418

Antioxidant (Ground) 419

Antioxidant (Whole Pill) 420

APIs with Aromatic Ring Structures in iPrep 421

Ascorbic Acid 422

Aspirin (Ground) 423

Aspirin (Whole) 424

Beta Carotene 425

Biotin 426

Calcium Carbonate 427

Calpan Pantothenic Acid 428

Cephalexin 429

Chromium Chelate 430

Cold & Flu Medicine (Liquid) 431

Cupric Sulfate 432

Diclofenac K 433

Empty Capsule (Gel) 434

Erythromycin 435

Fish Oil (No Capsule) 436

Flurbiprofen 437

Folic Acid 438

Ginkgo (Ground) 439

Ginkgo (Whole Pill) 440

Hydrocodone 441

Ibuprofen (Ground) 442

Kelp 443

Losartan K 444

Magnesium Carbonate 445

Magnesium Oxide 446

Manganese Carbonate 447

Medroxyprogesterone 448

Metformin (Ground) 449

Mineral Oil 450

Multivitamin (Ground) 451

Multivitamin (Whole) 452

Naproxen Sodium 453

Niacinamide 454

Pantothenic Acid 455

Potassium Chelate 456

Protopic Ointment 457

Pyridoxine 458

Riboflavin 459

Selenium Chelate 460

Sleep Aid 461

Sodium Polystyrene Sulfonate in iPrep 462

Stearic Acid 463

Sudan Orange (4-Fluorophenyl) in iPrep 464

Thiamine 465

Transdermal Patch 466

Trixie Phosphate in iPrep 467

Vitamin B-12 469

Vitamin C (Ground) 470

Vitamin C (Whole) 471

Vitamin Concentrate (High Fe) 472

Vitamin D 473

Vitamin D3 474

Vitamin E 475

Vitamins 476

Zinc Sulfate 477

Plastics, Polymers, & Oils

Acrylamide 479

Brominated Flame Retardant in iPrep 480

Bunker Oil in iPrep 481

Char - Sulfuric Acid 482

Crude Oil 483

Diesel Fuel 484

Epoxy Hardener 485

Fatty Alcohol 486

Fluorinated Ethylene Propylene (FEP) in iPrep 487

Fuel Oil (Slurry) in iPrep 488

Gasoline 490

Glycerol Ester of Wood Rosin (Ground) in iPrep 491

HDPE 492

Kerosene 493

Kevlar in iPrep 494

Lube Oil 495

Methyl Ester of Hydrogenated Wood Rosin (Ground) in iPrep 496

Mineral Oil 497

Motor Oil (New) 498

Motor Oil (Waste) 499

Motor Oil (Waste) in iPrep 500

Nylon 501

Oil Wear Metals 502

PET (Polyethylene Terephthalate) 503

PET (Polyethylene Terephthalate) in iPrep 504

Petrolatum 505

Plasticizer (Filter) in iPrep 506

Plasticizers in iPrep 507

Polycarbonate Resin 508

Polyethersulfone (Filter Paper) 510

Polyethylene - High Density 511

Polyphenylene Sulfide 512

Polypropylene 513

Polyterpene Resin in iPrep 514

Polyurethane 515

Polyurethane in iPrep 516

PVC (Polyvinyl Chloride Resin) 517

Rubber 518

Rubber (Synthetic) 519

Silane Terminated Polyether in iPrep 520

Silicone Tubing - TiO2 Filler 521

Styrene Acrylate 522

Terephthalic Acid 523

Wax (Petroleum Based) 524

Water & Wastewater Treatment

NPDES (Waste Water) 526

Sludge (Industrial) 527

TCLP Extract 528

US EPA 3015 (Aqueous Samples) 529

US EPA 3015a (Aqueous Sample) 530

Waste Activated Sludge 531

Water (For Analysis of Phosphorous) 532

Agriculture

Microwave Digestion ofAlfalfa

AgriculturMARS 6

* Ramp times and power may vary depending on the type and number of vessels.

Method TypeOne Touch

Control TypeRamp to Temperature

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 g

ReagentsHNO₃

Recommended VesselsEasyPrepEasyPrep PlusMARSXpressMARSXpress Plus

Recommended EquipmentMARS 6MARS 6 iWave

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

Stage Temp (°C) *Ramp (mm:ss) Hold (mm:ss) Pressure (psi) * Power (W) Stirring

1 200 15:00 15:00 800 900-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

ResultsSample was clear, colorless, and particle free upon dilution to 50 mL.

Microwave Digestion ofAlfalfa

Microwave Digestion ofBoric Acid HF Neutralization

AgriculturMARS 6

Sample TypeOrganic

Heating Program

Max Sample WeightVaries by Sample

ReagentsH₃BO₃ (4%)

Recommended VesselsEasyPrepEasyPrep PlusMARSXpress Plus

NotesThis procedure can be used if it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed by reaction of certain analytes with hydrofluoric acid.

Alternatively, 1-4 g of solid H₃BO₃ + 25 mL deionized H₂O can be used in place of the 4% w/v solution.

ProcedureAllow vessel to cool. Add 30 mL H₃BO₃ (4%) into the vessel that contains the sample and acid.

1 170 15:00 15:00 800 900-1800 Off

ResultsSee sample specific method notes.

Microwave Digestion ofCannabis (Plant)

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃, or alternatively 9 mL HNO₃, and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 10:00 800 900-1800 Off

Microwave Digestion ofCoffee Beans

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may; however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of ground sample into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 15:00 800 900-1800 Off

ResultsSample was clear, colorless, and particle free upon dilution to 50 ml.

Microwave Digestion ofCotton

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 off

Microwave Digestion ofFeed Grain

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureWeigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO₃, and 2 ml of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 off

Microwave Digestion ofFeed Grain

Microwave Digestion ofFertilizer - AOAC Method 2017.02

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

NotesThe addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis. This method may not provide a total digest of all fertilizer samples.

ProcedureWeigh 1 g (0.5 g for organic matrices) of the sample into the digestion vessel. Add 9 mL of HNO₃, and 3 mL HCl. Gently swirl the mixture and wait approximately 20 minutes before closing the vessel.

1 200 15:00 20:00 800 900-1800 Off

ResultsSample was clear, colorless, with some particles (silicates) remaining upon dilution to 50 mL.

Microwave Digestion ofFertilizer -AOAC Method 2006.03

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis method may not provide a total digest of all fertilizer samples.

ProcedureWeigh 1 g (0.5 g for organic matrices) of the sample into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 20:00 800 900-1800 Off

Microwave Digestion ofGeranium

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900 -1800 Off

Microwave Digestion ofGeranium

Microwave Digestion ofImpatiens

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofImpatiens

Microwave Digestion ofLeather

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofLeather

Microwave Digestion ofPalm Leaves

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO₃, and 2 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPalm Leaves

Microwave Digestion ofPlant Tissue

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis. Some plant tissues contain silicates which would require HF for total dissolution.

1 200 15:00 15:00 800 900-1800 off

Microwave Digestion ofSoybean

AgriculturMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 g (Dry Weight)

ReagentsHNO₃

ProcedureWeigh 0.5 g of (dry weight) sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 off

Microwave Digestion ofSoybean Meal

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSoybean Meal

Microwave Digestion ofSpinach Leaves

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 off

Microwave Digestion ofStraw

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

Recommended VesselsEasyPrepEasyPrep Plus

NotesThe above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric HF Neutralization” The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 ml of HNO₃, and 1 ml of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofStraw

Microwave Digestion ofTea Extract

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO₃ and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 10:00 800 900-1800 Off

Microwave Digestion ofTea Extract

Microwave Digestion ofTea Tannin

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 9 mL HNO₃ and 1 mL HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 10:00 800 900-1800 Off

Microwave Digestion ofTea Tannin

Microwave Digestion ofTobacco

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe use of HF may be required to digest any silicates found in the sample.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofTobacco

Microwave Digestion ofWheat

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWood

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWood Pulp

AgriculturMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 g (Wet Weight)

ReagentsHNO₃

ProcedureWeigh 0.5 g (wet weight) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWood Pulp

Microwave Digestion ofWool

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWool

Microwave Digestion ofYeast

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1.0 g of dry weight (0.5 g w/Xpress vessels) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofYeast

Clinical & Biological

Microwave Digestion ofAnimal Tissue (Dry)

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may; however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g (0.25 g w/MARSXpress Vessels) into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAnimal Tissue (Dry)

Microwave Digestion ofAnimal Tissue (Wet)

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 1 g (0.5 g w Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAnimal Tissue (Wet)

Microwave Digestion ofBeef Bone

Clinical aMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 g (Ground)

ReagentsHNO₃

ProcedureWeigh 0.5 g (ground) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBeef Bone

Microwave Digestion ofBlood (Human)

Clinical aMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight2 mL

ReagentsHNO₃ H₂O₂

NotesTake caution when mixing the acids and the sample. The sample may foam slightly.

ProcedureTransfer 2 mL of the sample into the digestion vessel. Add 5 mL of HNO₃ and 2 mL of H₂O₂. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Clinical aMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 mL

ReagentsHNO₃

Recommended Vessels10 mL MARSXpress20 mL MARSXpress

ProcedureTransfer 0.5 mL of the sample into the digestion vessel. Add 2 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 N/A 1030-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.c) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofBovine Blood

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureTransfer 1 mL of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBovine Blood

Microwave Digestion ofBovine Liver (Wet)

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1.0 g (wet weight )(0.5 g w/Xpress Vessels) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBovine Liver (Wet)

Microwave Digestion ofBrain Tissue (Dog)

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1 g (wet weight) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBrain Tissue (Dog)

Microwave Digestion ofDog Feces (Dry)

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.2 g (dry weight) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofDog Feces (Dry)

Microwave Digestion ofFingernails

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.1 g (0.05 g if using the 10 mL vessel) of the sample into the digestion vessel. Add 4 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 180 15:00 15:00 N/A 900-1800 Off

ResultsSample was clear, colorless and particle free upon dilution to 50 mL.

Microwave Digestion ofFingernails

Microwave Digestion ofHair

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.1 g (0.05 g if using the 10 mL vessel) of the sample into the digestion vessel. Add 4 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 180 15:00 15:00 N/A 900-1800 Off

Microwave Digestion ofHair

Microwave Digestion ofLobster Hepatopancreas (Tort-CRM)

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1.0 g (dry weight) (0.5 g w/Xpress Vessels) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofLobster Hepatopancreas (Tort-CRM)

Microwave Digestion ofRat Kidney

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1 g (wet weight) of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofRat Kidney

Microwave Digestion ofUrine - Human

Clinical aMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃H₂O₂

NotesTake caution when mixing the acids and the sample. The sample may foam slightly.

ProcedureTransfer 4 ml of the sample into the digestion vessel. Add 4 mL of HNO₃, and 2 mL of H₂O₂. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofUrine - Human

Consumer Products

AgriculturMARS 6

Sample TypeOrganic

Heating Program

1 170 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCandle Fragrance

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

Recommended VesselsiPrep

Recommended EquipmentMARS 6 iWave

NotesThis application can only be run in the iPrep vessel.

The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution.The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureAdd 0.1 g of the sample into the digestion vessel. Add 7 mL of HNO₃ and 1 mL HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 250 30:00 30:00 N/A 700-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

ResultsSample was clear and colorless upon dilution to 50 mL.

Microwave Digestion ofCandle Fragrance in iPrep

Microwave Digestion ofCosmetics (Liquid Make-Up)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 8 mL of HNO₃ and 2 mL of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 off

Microwave Digestion ofDeodorant (Powder)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 210 25:00 15:00 800 900-1800 Off

Microwave Digestion ofDeodorant (Powder)

Microwave Digestion ofEye Shadow

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 7 mL of HNO₃ and 3 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

ResultsSample was clear, colorless, and particle free upon dilution to 50 mL

Microwave Digestion ofFoam (Mattress)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃H₂SO₄

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL HNO₃ and 1 mL of H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 250 30:00 15:00 N/A 700-1800 Off

Microwave Digestion ofFoam (Mattress) in iPrep

Microwave Digestion ofSanitizers (Hand)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃

NotesAcid addition should be added slowly and in aliquots of 1 mL, as the reaction is vigorous and exothermic upon addition of HNO₃.The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution.The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 1 mL of HCl and 4 mL of HNO₃ in aliquots of 1 mL. Gently swirl after each aliquot is added and allow reaction to subside before adding more HNO3. Allow approximately 45 minutes for pre-digestion.

1 190 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSanitizers (Hand)

Microwave Digestion ofShampoo (Dandruff)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSoaps (Lotion and Foam)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 190 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSoaps (Lotion and Foam)

Microwave Digestion ofSunscreen

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

1 200 30:00 15:00 N/A 900-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.e) If using HF, follow restrictions listed in HF Addendum.

Microwave Digestion ofSunscreen in iPrep

Microwave Digestion ofSuppository (Capsule)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Capsule (Approx 1 g)

ReagentsHNO₃

NotesEnsure that the capsule is completely covered with reagent before sealing the vessel.

ProcedureWeigh 1 Capsule (approx. 1.0 g) into the digestion vessel. Add 12 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSuppository (Capsule)

Microwave Digestion ofToothpaste

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.1 g of the sample onto a filter disk and insert into the digestion vessel. Add 8 mL HNO₃ and 2 mL HF. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 200 20:00 15:00 800 1030-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) The control / reference vessel must contain the largest and most reactive sample.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.e) If using HF, follow restrictions listed in HF Addendum.

Microwave Digestion ofToothpaste

Microwave Digestion ofVaseline

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofVaseline

Microwave Digestion ofWax (Candle)

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWax (Candle)

Environmental & Regulatory

AgriculturMARS 6

Sample TypeOrganic

Heating Program

1 170 15:00 15:00 800 900-1800 Off

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

NotesThe addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis. This method may not provide a total digest of all fertilizer samples.

ProcedureWeigh 1 g (0.5 g for organic matrices) of the sample into the digestion vessel. Add 9 mL of HNO₃, and 3 mL HCl. Gently swirl the mixture and wait approximately 20 minutes before closing the vessel.

1 200 15:00 20:00 800 900-1800 Off

ResultsSample was clear, colorless, with some particles (silicates) remaining upon dilution to 50 mL.

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis method may not provide a total digest of all fertilizer samples.

ProcedureWeigh 1 g (0.5 g for organic matrices) of the sample into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 20:00 800 900-1800 Off

Microwave Digestion ofFly Ash

EnvironmMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsH₂SO₄ H₃PO₄

ProcedureAdd 0.1 g of the sample into the digestion vessel. Add 3 mL of H₂SO₄ and 3 mL of H₃PO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 260 35:00 30:00 N/A 700-1800 Off

Microwave Digestion ofFly Ash in iPrep (Step 1 of 2)

Microwave Digestion ofFly Ash

EnvironmMARS 6 (

ProcedureAllow vessels to cool. Open and add 1.5 mL of HNO₃, 1.5 mL of HCl, and 1.5 mL of HF. Wait approximately 15 minutes before closing the vessel.

Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

ReagentsHNO₃HClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization". The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

Heating Program

Sample TypeOrganic

1 230 30:00 30:00 N/A 700-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofFly Ash in iPrep (Step 2 of 2)

Microwave Digestion ofLimestone

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

NotesAllow any initial reaction to subside before sealing the vessel.

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve in soluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 7.5 mL of HNO₃ and 2.5 ml of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

ResultsSample was clear, colorless and particle free upon dilutionto 50 ml.

Microwave Digestion ofLimestone

Microwave Digestion ofNPDES (Waste Water)

EnvironmMARS 6

Sample TypeWater

Heating Program

ReagentsHNO₃ HCl

Recommended Vessels75 mL MARSXpressEasyPrepEasyPrep PlusMARSXpress Plus

ProcedureTransfer 50 mL of the sample into the digestion vessel. Add 3 mL of HNO₃, and 2 mL HCl. Gently swirl the mixture before closing the vessel.

1 165 30:00 0:00 800 900-1800 Off

Microwave Digestion ofPine Needles

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃H₂O₂ (30%)

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 8 mL of HNO₃ and 2 mL of H₂O₂ (30%). Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPine Needles

Microwave Digestion ofRoHS (For Pb, Hg, and Cd Analysis)

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ H₂SO₄

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 10 mL of HNO₃, and 0.02 mL of H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 10:00 15:00 800 900-1800 Off

Microwave Digestion ofRoHS (For Pb, Hg, and Cd Analysis)

Microwave Digestion ofSediment (BCCS - 1 CRM) (Leach)

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide complete digestion of some sample matrixes. The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSediment (BCCS - 1 CRM) (Leach)

Microwave Digestion ofSediment (Buffalo River) (Leach)

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide complete digestion of some sample matrixes. The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 175 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSediment (Buffalo River) (Leach)

Microwave Digestion ofSludge (Industrial)

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe use of HF may be required to digest any silicates found in the sample. The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSoil (Montana - CRM) (Leach)

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide complete digestion of some sample matrixes. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 175 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSoil (Montana - CRM) (Leach)

Microwave Digestion ofTCLP Extract

EnvironmMARS 6

Control TypeStandard Control

Sample TypeWater

Heating Program

ReagentsHNO₃

Recommended VesselsEasyPrep PlusMARSXpressMARSXpress Plus

ProcedureTransfer 25 mL of the sample into the digestion vessel. Add 5 mL of HNO₃. Gently swirl the mixture before closing the vessel. If a high organic content is suspected, a 10 ml sample volume is recommended.

1 170 10:00 10:00 800 900-1800 Off

Microwave Digestion ofUS EPA 3015 (Aqueous Samples)

EnvironmMARS 6

Sample TypeWater

Heating Program

ReagentsHNO₃

Recommended Vessels75 mL MARSXpressEasyPrep PlusMARSXpress Plus

ProcedureTransfer 45 mL of the sample into the digestion vessel. Add 5 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 170 10:00 10:00 800 900-1800 Off

ResultsThis method is intended to be an acid leach, not a total digest. Hydrofluoric acid will be required to provide complete digestion of the sample matrix.

Microwave Digestion ofUS EPA 3015a (Aqueous Sample)

EnvironmMARS 6

Sample TypeWater

Heating Program

ReagentsHNO₃HCl (Optional)

ProcedureTransfer 45 mL of the sample into the digestion vessel. Add 5 mL of HNO3, or alternatively 4 mL of HNO₃ and 1 mL HCl. Gently swirl the mixture before closing the vessel.

1 170 10:00 10:00 800 900-1800 Off

Microwave Digestion ofUS EPA 3051 (Solid Sample)

EnvironmMARS 6

Sample TypeSolid

Heating Program

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 175 5:30 4:30 800 900-1800 Off

ResultsThis method is intended to be an acid leach, not a total digest.

Microwave Digestion ofUS EPA 3051 (Solid Sample)

Microwave Digestion ofUS EPA 3051a (Solid Sample)

EnvironmMARS 6

Sample TypeSolid

Heating Program

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃, or alternatively 9 mL HNO₃, and 1 mL HCl. Gently swirl the mixture before closing the vessel.

1 175 5:30 4:30 800 900-1800 Off

ResultsThis method is intended to be an acid leach, not a total digest.

Microwave Digestion ofUS EPA 3051a (Solid Sample)

Microwave Digestion ofUS EPA 3052 (Sludge, Soil, Sediment or Oil)

EnvironmMARS 6

Sample TypeSolid

Heating Program

ReagentsHNO₃HF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the method note entitled “Boric Acid HF Neutralization”.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO₃, and 3 mL HF. Gently swirl the mixture before closing the vessel.

1 180 5:30 9:30 800 900-1800 Off

Microwave Digestion ofUS EPA 3052 (Sludge, Soil, Sediment or Oil)

Microwave Digestion ofUSP 232/233 (Pharmaceuticals)

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 9 mL of HNO₃, and 1 mL HCl. Gently swirl the mixture before closing the vessel.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofWaste Activated Sludge

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 0.5 g (dry weight) (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 5 mL of HNO₃, and 5 mL of H₂O₂. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWater (For Analysis of Phosphorous)

EnvironmMARS 6

Method TypeClassic

Sample TypeWater

Heating Program

ReagentsK₂S₂O₈ H₂SO₄

Recommended EquipmentMARS 6

ProcedureTransfer 50 mL of H₂O into the digestion vessel. Add 0.5 g of K₂S₂O₈ and 1 mL of H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 170 15:00 15:00 800 900-1800 Off

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Food Testing

Microwave Digestion ofAlum

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization".

The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 3 mL of HCl, 5 mL of HF, and 2 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 10:00 800 900-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain therequired results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at orbelow room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vesselsdetected.

Microwave Digestion ofAlum

Microwave Digestion ofApple

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofApple

Microwave Digestion ofAsparagus

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 (Dry Weight)

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Resultssample was clear, colorless, and particle free upon dilution to 50 mL.

Microwave Digestion ofAsparagus

Microwave Digestion ofAspartame

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 240 30:00 15:00 N/A 700-1800 Off

Microwave Digestion ofAspartame in iPrep

Microwave Digestion ofBacon (Cooked)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃DI H₂O

ProcedureWeigh 1.0 g of the sample into the digestion vessel. Add 8 mL of HNO₃ and 2 mL of DI H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBacon (Cooked)

Microwave Digestion ofBeef (Ground)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBeef (Ground)

Microwave Digestion ofBeef Bouillon

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBeef Bouillon

Microwave Digestion ofBeer (Light)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBeer (Light)

Microwave Digestion ofBlueberry

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBlueberry

Microwave Digestion ofBroccoli

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 20 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBroccoli

Microwave Digestion ofCaramel Color

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCaramel Color

Microwave Digestion ofCarrot

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCarrot

Microwave Digestion ofCelery

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCelery

Microwave Digestion ofCheese (Cracker)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of ground sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofCheese (Cracker)

Microwave Digestion ofCheese (Powder)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCheese (Powder)

Microwave Digestion ofCheese (Processed)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofCheese (Processed)

Microwave Digestion ofCherry

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCherry

Microwave Digestion ofChewing Gum

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis. The use of HF may be required to digest any silicates found in the sample.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofChewing Gum

Microwave Digestion ofChicken (Boneless)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofChicken (Boneless)

Microwave Digestion ofChili Candy (Mexican)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofChili Candy (Mexican)

Microwave Digestion ofChili Powder

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight2 g

The use of HF may be required to digest any silicates found in the sample.

ProcedureWeigh 2 g of the sample into the digestion vessel. Add 10 mL HNO₃ and 5 mL deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 240 30:00 30:00 N/A 700-1800 Off

ResultsSample was clear and colorless with some small particles (silicates) remaining upon dilution to 50 mL.

Microwave Digestion ofChili Powder in iPrep

Microwave Digestion ofChips (Potato)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofChips (Potato)

Microwave Digestion ofCitrus Leaves

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃DI H₂OHF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 6 mL of HNO₃, 3 mL of deionized H₂O, and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofClam Puree

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofClam Puree

Microwave Digestion ofCocoa

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCocoa

Microwave Digestion ofCoconut

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCoconut

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may; however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of ground sample into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 15:00 800 900-1800 Off

Microwave Digestion ofCucumber

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCucumber

Microwave Digestion ofDog Food

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of (dry weight) up to 1 g (wet weight) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 Off

ResultsSample was clear, colorless, and particle free upon dilution to 50 Ml

Microwave Digestion ofDog Food

Microwave Digestion ofFish Tissue

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g (dry weight) up to 2 g (wet weight) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFish Tissue

Microwave Digestion ofFlounder

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFlounder

Microwave Digestion ofFlour

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFlour

Microwave Digestion ofFood

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g dry weight (0.25 g w/Xpress vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃ . Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFood

Microwave Digestion ofFood Coloring (Liquid)

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 mL.

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFood Coloring (Liquid)

Microwave Digestion ofFruit Juice

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFruit Juice

Microwave Digestion ofGelatin

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGelatin

Microwave Digestion ofGranola Bar

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of ground sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofGranola Bar

Microwave Digestion ofGrape

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGrape

Microwave Digestion ofGrapefruit

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGrapefruit

Microwave Digestion ofGummy Bear

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofGummy Bear

Microwave Digestion ofHam

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofHam

Microwave Digestion ofInfant Cereal (Rice Grain)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofInfant Cereal (Rice Grain)

Microwave Digestion ofInfant Formula (Liquid)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis application can only be run in the iPrep vessel. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureTransfer 2 mL of of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 210 30:00 20:00 N/A 700-1800 Off

Results Sample was clear, colorless and particle free upon dilution to 50 mL

Microwave Digestion ofInfant Formula (Liquid) in iPrep

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureTransfer 1 mL (0.5 mL w/Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofInfant Formula (Powder)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 210 20:00 20:00 N/A 700-1800 Off

Microwave Digestion ofInfant Formula (Powder) in iPrep

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofKidney Bean

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 (Dry Weight)

ReagentsHNO₃

ProcedureWeigh 0.5 g (dry weight) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofKidney Bean

Microwave Digestion ofKielbasa Sausage

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofKielbasa Sausage

Microwave Digestion ofMaple Syrup

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofMaple Syrup

Microwave Digestion ofMayonnaise

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofMayonnaise

Microwave Digestion ofMeal Replacement Shake (Chocolate)

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 g (Dry Mix)

ReagentsHNO₃

ProcedureWeigh 0.5 g (dry mix) of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMeal Replacement Shake (Chocolate)

Microwave Digestion ofMilk (Whole - Liquid)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMilk (Powder)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofNutritional Drink (Adult)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofNutritional Drink (Adult)

Microwave Digestion ofOlive Oil

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofOlive Oil

Microwave Digestion ofOrange Juice

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureTransfer 2.5 ml of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofOrange Juice

Microwave Digestion ofOyster Puree

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1 g (wet weight) of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofOyster Puree

Microwave Digestion ofPeanut Butter

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofPeanut Butter

Microwave Digestion ofPear

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPear

Microwave Digestion ofPepper (Bell, Chili, Etc.)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPepper (Bell, Chili, Etc.)

Microwave Digestion ofPistachio Nuts

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPistachio Nuts

Microwave Digestion ofPizza (Frozen, Pepperoni)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1.0 g (homogenized) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPizza (Frozen, Pepperoni)

Microwave Digestion ofPlum

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPlum

Microwave Digestion ofPork (Ground)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPork (Ground)

Microwave Digestion ofPotato

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPotato

Microwave Digestion ofPotato Chips

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 g (Ground)

ReagentsHNO₃

ProcedureWeigh 0.5 g (ground) of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPotato Chips

Microwave Digestion ofPretzel (Salted)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPretzel (Salted)

Microwave Digestion ofProtein Bar

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofProtein Bar

Microwave Digestion ofRaspberry

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g (dry weight) of the sample into the digestion vessel. Add 10 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofRaspberry

Microwave Digestion ofRice

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofRice

Microwave Digestion ofSafflower Oil

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSafflower Oil

Microwave Digestion ofSalad Dressing (Ranch)

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.5 g (Dry Mix)

ReagentsHNO₃

ProcedureWeigh 0.5 g (dry mix) of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSalad Dressing (Ranch)

Microwave Digestion ofSalami

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofSalami

Microwave Digestion ofSausage

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofSausage

Microwave Digestion ofSoda (Diet)

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight 1 mL

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSoda (Diet)

Microwave Digestion ofSodium Free - Seasoning

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSodium Free - Seasoning

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of (dry weight) sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 off

Microwave Digestion ofSpinach

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSpinach

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis. Some plant tissues contain silicates which would require HF for total dissolution.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofSpinach Pasta

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSpinach Pasta

Microwave Digestion ofStrawberry

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofStrawberry

Microwave Digestion ofSugar (Granulated)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSugar (Granulated)

Microwave Digestion ofTea (Chai)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ DI H₂O

ProcedureWeigh 2.5 g of the sample into the digestion vessel. Add 10 mL HNO₃ and 5 mL deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 240 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofTea (Chai) in iPrep

Microwave Digestion ofTea Leaves

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofTea Leaves

Microwave Digestion ofTomato Leaves

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesSome plant tissues contain silicates which would require HF for total dissolution.

1 200 15:00 15:00 800 900-1800 Off

ResultsSample was clear, colorless, with white silica particles upon dilution to 50 mL.

Microwave Digestion ofTomato Leaves

Microwave Digestion ofTomato Paste (70% Moisture)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 2 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofTomato Paste (70% Moisture)

Microwave Digestion ofTomato Soup

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofTomato Soup

Microwave Digestion ofWalnut

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g (dry weight) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWalnut

Microwave Digestion ofWatermelon

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWatermelon

Microwave Digestion ofWheat Crackers

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWheat Crackers

Microwave Digestion ofWhey

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWhey

Microwave Digestion ofWhey (Powder)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWine

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofWine

Microwave Digestion ofYogurt (Plain)

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofYogurt (Plain)

Geoscience & Mining

Microwave Digestion ofAlpha - Alumina

GeoscienMARS 6

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHCl

The maximum number of vessels that can be run for this method is 6.

Ensure that particles are well suspended in acid mixture prior to sealing vessel.

Stirring may be helpful to keep particles suspended in solution during digestion.

ProcedureWeigh 0.5 g of sample into the digestion vessel. Add 10 mL HCl. Gently swirl the vessel to thoroughly mix the sample and acid.

1 240 30:00 59:59 N/A 900-1800 N/A

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain therequired results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at orbelow room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as One Touch, the ramp time and power will be automatically determined based on the number and type of vesselsdetected.

Microwave Digestion ofAlpha - Alumina in iPrep

Microwave Digestion ofAlumina Beads (w/ Silver substrate)

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsH₃PO₄H₂SO₄

NotesThis application can only be run in the iPrep vessel

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 6.5 mL of H₃PO₄, and 3.5 mL of H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 280 30:00 45:00 N/A 700-1800 Off

Microwave Digestion ofAlumina Beads (with Silver Substrate) in iPrep (Step 1 of 2)

Microwave Digestion ofAlumina Beads (w/ Silver substrate)

GeoscienMARS 6 (

ProcedureAllow vessel to cool after completion of Step 1. Proceeding step one, add 3 mL of HNO₃, and 3 mL HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

ReagentsHNO₃HF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

Heating Program

Sample TypeOrganic

1 220 25:00 30:00 N/A 700-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.

Microwave Digestion ofAlumina Beads (with Silver Substrate) (Step 2 of 2)

Microwave Digestion ofAlumina Powder

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₃PO₄

ProcedureAdd 0.1 g of the sample into the digestion vessel. Add 8 mL of H₃PO₄. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 275 30:00 45:00 N/A 700-1800 Off

ResultsSample was clear, gold colored, and particle free upon dilution to 50 mL.

Microwave Digestion ofAlumina Powder in iPrep

Microwave Digestion ofAluminum Nitride

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₃PO₄ HCl

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 5 mL H₃PO₄ and 10 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 175 15:00 10:00 800 900-1800 Off

Microwave Digestion ofAluminum Nitride

Microwave Digestion ofAluminum Oxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

NotesEasyPrep Plus requires a high temperature probe for this method.

ProcedureWeigh 0.25 g of sample into the digestion vessel. Add 6.5 mL H₃PO₄ and 3.5 mL H₂SO₄. Gently swirl the vessel to thoroughly mix the sample and acid.

1 270 30:00 20:00 N/A 900-1800 N/A

Microwave Digestion ofAluminum Oxide (H₃PO₄)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.1g

ReagentsH₃PO₄

This method may not provide a total digest of all samples. Hydrofluoric acid may be required to provide complete digestion of some sample matrixes.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 8 mL H₃PO₄. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 260 25:00 45:00 N/A 700-1800 Off

ResultsSample was clear and colorless with some white particles remaining upon dilution to 50 mL.

Microwave Digestion ofAluminum Oxide (H3PO4) in iPrep

Microwave Digestion ofAsbestos

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃HF

NotesHF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

ProcedureWeigh 0.3 g of the sample into the digestion vessel. Add 6 mL of HCl, 3 mL of HNO₃, and 3 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAutomotive Catalyst

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”. Reducing the particle size increases the efficiency of digestion and may decrease the digestion time.

ProcedureWeigh 0.4 g of the sample into the digestion vessel. Add 10 mL of HCl and 0.5 mL of HF. Gently swirl the mixture before closing the vessel.

1 210 15:00 30:00 800 900-1800 Off

Microwave Digestion ofAutomotive Catalyst

Microwave Digestion ofBarium Titanate (BaTiO₃)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesHF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel. This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 5 mL of HNO3 and 10 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 20:00 800 900-1800 Off

Microwave Digestion ofBarium Titanate (BaTiO3)

Microwave Digestion ofBastnaesite Ore

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃

This method could potentially form HF. It is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 9 mL of HCl and 3 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 30:00 800 900-1800 Off

Microwave Digestion ofBastnaesite Ore (Step 1 of 2)

Microwave Digestion ofBastnaesite Ore

GeoscienMARS 6 (

ProcedureAllow vessels to cool. Open and add 1 g solid Boric Acid and 20 mL deionized H₂O. Wait approximately 15 minutes before closing the vessel.

ReagentsSolid Boric AcidDI H₂O

Heating Program

Sample TypeOrganic

1 175 15:00 15:00 800 900-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

Microwave Digestion ofBastnaesite Ore (Step 2 of 2)

Microwave Digestion ofBauxite

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsH₃PO₄ H₂SO₄

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 6.5 mL of H₃PO₄ and 3.5 mL of H₂SO₄. Gently swirl the mixture before closing the vessel.

1 240 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBauxite (Step 1 of 2)

Microwave Digestion ofBauxite

GeoscienMARS 6 (

ProcedureProceeding step 1 add 1 mL of HNO₃, 1 mL of HCl, and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

ReagentsHNO₃HClHF

Heating Program

Sample TypeOrganic

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBauxite (Step 2 of 2)

Microwave Digestion ofBismuth Aluminate Mixture

GeoscienMARS 6

Method TypeClassic

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 9 mL HCl and 3 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 190 15:00 15:00 800 900-1800 Off

ResultsSample was clear, colorless, and particle free upon diltuion to 50 mL.

Microwave Digestion ofBismuth Yttrium Iron Oxide

GeoscienMARS 6 (

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHCLHNO₃HF

HF should be added slowly and carefully to the sample.

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 6 mL of HCl, 2 mL of HNO₃, and 0.5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Allow any initial reaction to subside before sealing vessel.

1 230 25:00 15:00 N/A 700-1800 Off

Microwave Digestion ofBismuth Yttrium Iron Oxide in iPrep (Step 1 of 2)

GeoscienMARS 6 (

ProcedureAllow vessel to cool. Add 1.75 g of solid H₃BO₃ + 20 mL deionized H₂O into the vessel that contains the sample and acid.

ReagentsSolid H₃BO₃DI H₂O

Heating Program

Method TypeClassic

Sample TypeStandard

1 170 25:00 10:00 N/A 700-1800 Off

General Precautiona) If using HF, follow restrictions listed in HF Addendum.b) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.d) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

ResultsSample was clear, yellow in color, and particle free upon dilution to 50 mL.

Microwave Digestion ofBoiler Scale (80-90% Fe)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBoiler Scale (80-90% Fe)

AgriculturMARS 6

Sample TypeOrganic

Heating Program

1 170 15:00 15:00 800 900-1800 Off

Microwave Digestion ofBoron Carbide (B₄C)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃H₂SO₄HF

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the One Touch Method note entitled “BoricAcid HF Neutralization”.

Reducing the particle size increases the efficiency of digestion and may decrease the digestion time.

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 5 mL of HNO₃ and 5 mL of H₂SO₄. Gently swirl to mix the sample particles in the acid. Slowly and carefully add 5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

Allow any initial reaction to subside before sealing vessel.

1 270 30:00 30:00 800 700-1800 Off

Microwave Digestion ofBoron Carbide (B4C) in iPrep

Microwave Digestion ofCarbon

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe structure, form and surface area of carbon samples varies widely. Higher temperatures and use of other reagents may be necessary in order to digest certain samples.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 10 mL of HNO₃ . Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 215 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCarbon

Microwave Digestion ofCement

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.3 g of the sample into the digestion vessel. Add 3 mL of HNO₃ , 6 mL of HCL, and 3 mL of HF. Gently swirl the mixture before closing the vessel.

1 175 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCement

Microwave Digestion ofCeramic (Fused Silica)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

NotesHF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing the vessel. This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Slowly add 2 mL of HNO₃ , and 8 mL of HF. Gently swirl the mixture before closing the vessel.

1 190 10:00 20:00 800 900-1800 Off

Microwave Digestion ofCeramic (Fused Silica)

Microwave Digestion ofCeramics

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0. 2 g of the sample into the digestion vessel. Add 5 mL of HNO₃ , and 5 ml of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCeramics

Microwave Digestion ofChar - Sulfuric Acid

GeoscienMARS 6

Sample TypeOrganic

Heating Program

Max Sample WeightVaries by sample

ReagentsH₂SO₄

NotesEasyPrep Plus requires a high temperature probe for this method. This method is for the pretreatment of large sample sizes or difficult organic samples that are resistant to oxidation. After the char is complete, the vessel is opened and a normal oxidation with HNO₃ can be run, usually at around 200C.

ProcedureAdd 6 mL H₂SO₄ into the vessel that contains the sample and acid.

1 260 15:00 30:00 800 900-1800 Off

Microwave Digestion ofChromium Carbide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃ and 10 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofChromium Carbide

Microwave Digestion ofChromium Oxide (Cr₂O₃)

GeoscienMARS 6

Method TypeClassic

Sample TypeStandard

Heating Program

Max Sample Weight50 mg

ReagentsH₂SO₄HNO₃H₂O₂ (30%)

Stirring is required for complete dissolution of this sample type.

ProcedureWeigh 50 mg of the sample into the digestion vessel. Insert stir bar into the vessel. Add 4.5 mL of H₂SO₄ and 4 mL of HNO₃ to the vessel, then 1.5 mL of H₂O₂ (30%) dropwise. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 280 30:00 59:59 N/A 900-1800 Med

2 280 0:00 30:01 N/A 900-1800 Med

ResultsSample was clear and turquoise colored upon dilution to 50 mL.

Microwave Digestion ofChromium Oxide (Cr2O3) in iPrep

Microwave Digestion ofClay

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHFHNO₃HCl

The addition of Conc. HCl (0-4 Ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution.

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 3 mL of HNO₃ , 1 mL of HCl, and 5 ml of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofClay

Microwave Digestion ofCoal

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis application can only be run in the iPrep vessel. The use of HF may be required to digest any silicates found in the sample. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 250 30:00 20:00 N/A 700-1800 Off

ResultsSample was clear, colorless with some particles remaining upon dilution to 50 mL.

Microwave Digestion ofCoal in iPrep

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe use of HF may be required to digest any silicates found in the sample. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 220 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCoal Ash

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”. The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.3 g of the sample into the digestion vessel. Add 3 mL of HNO₃ , 3 mL of HCl and 3 ml of HF. Gently swirl the mixture before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCoal Ash

Microwave Digestion ofCryolite / Electrolytic Bath

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesCryolite / Electrolytic Bath is approximately 55% Cryolite (Na₃AlF₆) and 30% Chiolite (Na₅Al₃F₁₄).

ProcedureWeigh 0.5g of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCryolite / Electrolytic Bath (Step 1 of 2)

Microwave Digestion ofCryolite / Electrolytic Bath

GeoscienMARS 6 (

ProcedureAllow vessel to cool after completion of Step 1. Open and add 30 mL of Deionized Water and 2 g Boric Acid (solid). Gently swirl the mixture and seal the vessel.

ReagentsDI H₂OBoric Acid (solid)

Heating Program

Sample TypeOrganic

1 175 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCryolite / Electrolytic Bath (Step 2 of 2)

Microwave Digestion ofFeldspar

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHCl HF

NotesThis application can only be run in the iPrep vessel. This procedure uses hydrofluoric acid. It is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used.

The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 2 mL of HCl and 8 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides

1 230 30:00 25:00 N/A 700-1800 Off

Microwave Digestion ofFeldspar in iPrep (Step 1 of 2)

Microwave Digestion ofFeldspar

GeoscienMARS 6 (

ProcedureAllow vessels to cool. Open and add 4 g solid boric acid and 30 mL of deionized H2O. Wait approximately 15 minutes before closing the vessel.

ReagentsSolid Boric AcidDI H₂O

Heating Program

Sample TypeOrganic

1 170 30:00 10:00 N/A 700-1800 Off

Microwave Digestion ofFeldspar (Step 2 of 2)

Microwave Digestion ofFerric Oxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃DI H₂O

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 10 mL of a 1:1:1 HCl, HNO₃, and Deionized H₂O mixture. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 25:00 20:00 N/A 700-1800 Off

Microwave Digestion ofFerric Oxide in iPrep

Microwave Digestion ofFurnace Slag

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”. The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureAdd 0.5 g of the sample into the digestion vessel. Add 3 mL of HNO₃, 2 mL of HCl, and 5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFurnace Slag

Microwave Digestion ofGadolinium Zirconium Oxide

GeoscienMARS 6 (

Method TypeClassic

Sample TypeStandard

Heating Program

ReagentsH₂SO₄HNO₃HF

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 4 mL of H₂SO₄, 1.5 mL of HNO₃ and 1.5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Allow any initial reaction to subside before sealing vessel.

1 280 35:00 30:00 N/A 700-1800 High

Microwave Digestion ofGadolinium Zirconium Oxide in iPrep (Step 1 of 2)

GeoscienMARS 6 (

ReagentsH₃BO₃DI H₂O

Heating Program

Method TypeClassic

Sample TypeStandard

1 170 25:00 10:00 N/A 700-1800 High

Microwave Digestion ofGas Atomized Powder

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 8 mL of HNO₃, 2 mL of HCl, and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 30:00 20:00 N/A 700-1800 Off

ResultsSamples were clear, green in color and particle free upon dilution to 50 mL.

Microwave Digestion ofGas Atomized Powder in iPrep

Microwave Digestion ofGlass Fiber

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

1 180 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGlass Fiber

Microwave Digestion ofHafnium Oxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl or H₂OHF

HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel. HNO3:HF:H2O (1:1:1) can be substituted for HNO3:HCl:HF (1:1:1) using the same heating program if the use of HCl is not desired. Mixtures are made from acids in their concentrated form.

ProcedureAdd 0.25 g of the sample into the digestion vessel. Add 10 mL of HNO₃:HCl:HF (1:1:1) or alternatively, HNO₃:H₂O:HF (1:1:1). (See Notes) Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 15:00 30:00 800 900-1800 Off

Microwave Digestion ofHafnium Oxide

Microwave Digestion ofIlmenite (FeTiO₃)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 5 mL of HNO₃, 1 mL of HCl, and 10 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofIlmenite (FeTiO3)

Microwave Digestion ofIron Ore

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂OHClHNO₃HF

NotesThe above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric HF Neutralization” The addition of Conc. HCl (0-8 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 1 g of the sample into the digestion vessel. Add 4 mL of H₂O, 8 ml of HCl, 4 mL of HNO₃, and 4 mL of HF. Gently swirl the mixture and wait approximately 15 minute before closing the vessel.

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofIron Ore

Microwave Digestion ofKaolin

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃HF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 3 mL of HCl, 1 mL of HNO3, and 7 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofKaolin

Microwave Digestion ofKerosene

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe pre-digestion step will allow the lighter fuel fractions to react before sealing the vessels.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofLead Sulfide (PbS)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 20:00 800 900-1800 Off

Microwave Digestion ofLithium Nickel Cobalt Manganese Oxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

Recommended VesselsMARSXpress

1 210 20:00 10:00 N/A 1030-1800 Off

ResultsThe samples were clear, green in color, and particle free upon dilution.

Microwave Digestion ofMagnesium Oxide (MgO 40 -54 %)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂O (deionized)HClHNO₃

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution.The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 5 mL of DI H₂O, 5 mL HCl, and 5 mL HNO₃. Gently swirl and allow approximately 15 minutes for pre-digestion.

1 200 20:00 20:00 800 900-1800 Off

Microwave Digestion ofMagnesium Oxide (MgO 40-54%)

Microwave Digestion ofManganous Oxide (MnO)

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.2g of the sample into the digestion vessel. Add 10 mL HNO₃ and 3 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 180 15:00 10:00 800 900-1800 Off

Microwave Digestion ofManganous Oxide (MnO) (Step 1 of 2)

GeoscienMARS 6 (

ProcedureAllow vessel to cool after completion of Step 1. Open and add 5 mL of HCl. Gently swirl the mixture and seal the vessel.

ReagentsHCl

Heating Program

Sample TypeOrganic

1 180 15:00 10:00 800 900-1800 Off

Microwave Digestion ofMica

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₃PO₄HClHF

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 4 mL of H₃PO₄, 3 mL of HCl, and 0.5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 230 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofMica in iPrep

Microwave Digestion ofMill Tailings

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

NotesEasyPrep Plus vessels require a high temperature probe for this method.

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 3 mL of H₃PO₄, and 2 mL of H₂SO₄. Gently swirl the mixture before closing the vessel.

1 270 15:00 30:00 800 900-1800 Off

Microwave Digestion ofMill Tailings (Step 1 of 2)

Microwave Digestion ofMill Tailings

GeoscienMARS 6 (

ProcedureCool, vent and open vessel after step 1. Add 2.5 mL HNO₃, 2.5 mL HCl, 2.5 mL HF, and 2.5 ml H₂O. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing the vessel.

ReagentsHNO₃HClHFH₂O

NotesThe above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in method note entitled “Boric HF Neutralization” The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

Heating Program

Sample TypeOrganic

1 200 15:00 20:00 800 900-1800 Off

Microwave Digestion ofMill Tailings (Step 2 of 2)

Microwave Digestion ofMineral Ore

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HClHF

ProcedureWeigh 0.3 g of the sample into the digestion vessel. Add 3 mL of HNO₃, 3 mL of HCl, and 3 mL of HF. Gently swirl the mixture before closing the vessel.

1 210 15:00 10:00 800 900-1800 Off

Microwave Digestion ofMineral Ore (Step 1 of 2)

Microwave Digestion ofMineral Ore

GeoscienMARS 6 (

ProcedureProceeding step 1 add 25 mL of Boric Acid (4%). Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

Heating Program

Sample TypeOrganic

1 170 15:00 10:00 800 900-1800 Off

Microwave Digestion ofMineral Ore (Step 2 of 2)

Microwave Digestion ofMolybdenum Disulfide (MoS₂)

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureAdd 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofMolybdenum Disulfide (MoS2) (Step 1 of 2)

Microwave Digestion ofMolybdenum Disulfide (MoS₂)

GeoscienMARS 6 (

ProcedureAllow vessel to cool after completion of Step 1. Add 10 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

ReagentsHCl

Heating Program

Sample TypeOrganic

1 180 10:00 10:00 800 900-1800 Off

Microwave Digestion ofMolybdenum Disulfide (MoS2) (Step 2 of 2)

Microwave Digestion ofNickel (II) Hydroxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 210 20:00 10:00 N/A 1030-1800 Off

ResultsThe samples were clear, light green in color, and particle free upon dilution.

Microwave Digestion ofNiobium Pentoxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureAdd 1.0 g of the sample into the digestion vessel. Add 2 mL of HNO₃, and 10 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofNiobium Pentoxide

Microwave Digestion ofPetroleum Coke

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsH₂SO₄

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 10 mL of H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 260 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPetroleum Coke (Step 1 of 2)

Microwave Digestion ofPetroleum Coke

GeoscienMARS 6 (

ProcedureProceeding step 1 add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

ReagentsHNO₃

Heating Program

Sample TypeOrganic

1 200 15:00 15:00 800 900-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.

Microwave Digestion ofPetroleum Coke (Step 2 of 2)

Microwave Digestion ofPhosphate (21%)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂O₂ (30%)HClHNO₃

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 5 mL of H₂O₂ (30%), 5 mL HCl dropwise (wait for reaction to subside before adding next acid), and 5 mL HNO₃. Gently swirl the mixture and wait approximately 1 hour before closing the vessel.

1 210 20:00 20:00 800 900-1800 Off

Microwave Digestion ofPhosphate Rock

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HBF₄

ProcedureAdd 0.5 g of the sample into the digestion vessel. Add 5 mL of HNO₃, 5 mL of HBF₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPhosphate Rock

Microwave Digestion ofPollucite Ore

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 210 25:00 25:00 N/A 700-1800 Off

Microwave Digestion ofPollucite Ore in iPrep (Step 1 of 2)

GeoscienMARS 6 (

ProcedureAllow vessel to cool. Add 2.5 g H₃BO₃ and 25 mL of deionized H₂O into the vessel that contains the sample and acid.

ReagentsH₃BO₃ DI H₂O

Heating Program

Sample TypeOrganic

1 170 25:00 15:00 N/A 700-1800 Off

General Precautiona) If using HF, follow restrictions listed in HF Addendum.b) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.c) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

ResultsSample was clear and particle free upon dilution to 50 mL.

Microwave Digestion ofPyrophyllite (AlSi₂)5(OH)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPyrophyllite (AlSi2)5(OH)

Microwave Digestion ofQuartz Powder

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHF

ProcedureWeigh 1 g of the sample into the digestion vessel. Add 10 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 200 15:00 30:00 800 900-1800 Off

Microwave Digestion ofQuartz Powder

Microwave Digestion ofRock (Pulverized)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHFHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofRock (Pulverized)

Microwave Digestion ofRock, High Sulfide

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 220 30:00 15:00 N/A 700-1800 Off

Microwave Digestion ofRock, High Sulfide in iPrep (Step 1 of 2)

Microwave Digestion ofRock, High Sulfide

GeoscienMARS 6 (

ProcedureProceeding step 1 add 3 mL of HF, and 2 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

ReagentsHFHCl

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

Heating Program

Sample TypeOrganic

1 200 25:00 10:00 N/A 700-1800 Off

General Precautiona) If using HF, follow restrictions listed in HF Addendum.b) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.c) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.d) The control / reference vessel must contain the largest and most reactive sample.e) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.

Microwave Digestion ofRock, High Sulfide in iPrep (Step 2 of 2)

Microwave Digestion ofRutile Ore

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF H₃PO₄.

NotesThe above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric HF Neutralization”

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 4 mL of HNO₃, 3 mL of H₃PO₄, and 8 mL of HF. Gently swirl the mixture before closing the vessel.

1 240 15:00 20:00 800 900-1800 Off

Microwave Digestion ofRutile Ore

Microwave Digestion ofSand

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFHCl

NotesThe above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric HF Neutralization” The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSand

Microwave Digestion ofShale

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHFHNO₃HCl

ProcedureWeigh 0.5 g of sample into the digestion vessel. Add 7 mL of HF, 3 mL of HNO₃, and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 25:00 15:00 N/A 700-1800 Off

Microwave Digestion ofShale in iPrep

Microwave Digestion ofSilica Sand

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂OHNO₃HF

NotesAdd HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides. The above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric HF Neutralization” Reducing the particle size increase the efficiency of digestion and may decrease the digestion time.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 5 mL of H₂O, 3 mL of HNO₃, and 8 mL of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSilica Sand

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHF

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization".

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 2 mL of HCl and 8 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 230 30:00 25:00 N/A 700-1800 Off

ResultsThe sample was clear, colorless and particle free upon dilution to 50 mL.

Microwave Digestion ofSilica Sand in iPrep

Microwave Digestion ofSilicon Dioxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 2 mL of HNO₃, and 8 mL of HF. Gently swirl the mixture before closing the vessel.

1 180 10:00 15:00 800 900-1800 Off

Microwave Digestion ofSilicon Dioxide

Microwave Digestion ofSilicon Nitride

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureAdd 0.1 g of the sample into the digestion vessel. Add 1 mL of HNO₃ and 5 mL HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 230 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofSilicon Nitride in iPrep

Microwave Digestion ofSilicon Wafer

GeoscienMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Piece (Approx 0.5 g)

ReagentsHNO₃HF

ProcedureAdd 1 piece (approx. 0.5 g) of the sample into the digestion vessel. Add 6 mL of HF and 3 mL of HNO3. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 10:00 15:00 800 900-1800 Off

Microwave Digestion ofSilicon Wafer

Microwave Digestion ofSlag - Furnace

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

NotesThe above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric HF Neutralization” The addition of Conc. HCl (0-4mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSlag - Furnace

Microwave Digestion ofSodium Antimonate

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃HF

ProcedureWeigh 0.5 g of sample into the digestion vessel. Add 10 mL of HCl, 3 mL of HNO₃, and 2 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 30:00 15:00 N/A 700-1800 Off

Microwave Digestion ofSodium Antimonate in iPrep

Microwave Digestion ofSpodumene (Lithium Feldspar)

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃HF

ProcedureWeigh .25 g of the sample into the digestion vessel. Add 3 mL of HCl, 1 mL of HNO₃ and 4 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 20:00 800 900-1800 Off

Microwave Digestion ofSpodumene (Lithium Feldspar) (Step 1 of 2)

Microwave Digestion ofSpodumene (Lithium Feldspar)

GeoscienMARS 6 (

ProcedureAllow vessel to cool after completion of Step 1. Open and add 30 mL of Deionized H₂O and 2 g H₃BO₃ (solid). Gently swirl the mixture and seal the vessel.

ReagentsDeionized H₂OH₃BO₃ (solid)

Heating Program

Sample TypeOrganic

1 170 15:00 10:00 800 900-1800 Off

Microwave Digestion ofSpodumene (Lithium Feldspar) (Step 2 of 2)

Microwave Digestion ofSulfide Bulk Concentrate

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofSulfide Bulk Concentrate in iPrep (Step 1 of 2)

Microwave Digestion ofSulfide Bulk Concentrate

GeoscienMARS 6 (

ProcedureProceeding step 1 add 5 mL of HCl, and 5 mL of Deionized Water. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

ReagentsHClDI H₂O

Heating Program

Sample TypeOrganic

1 200 30:00 10:00 N/A 700-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.

Microwave Digestion ofSulfide Bulk Concentrate in iPrep (Step 2 of 2)

Microwave Digestion ofTantalum Pentoxide (Ta₂O₅)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.5 g of sample into the digestion vessel. Add 3 mL of HNO₃, and 7 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 25:00 20:00 N/A 700-1800 Off

Microwave Digestion ofTantalum Pentoxide (Ta2O5) in iPrep

Microwave Digestion ofTitanium Dioxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofTitanium Slag Ore

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.25 g of sample into the digestion vessel. Add 5 mL of HNO₃, and 10 mL of HF. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing the vessel.

1 240 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofTitanium Slag Ore in iPrep

Microwave Digestion ofTungsten Carbide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Slowly add 5 mL of HNO₃, and 10 mL of HF. Gently swirl the mixture before closing the vessel.

1 220 20:00 20:00 800 900-1800 Off

Microwave Digestion ofTungsten Carbide

Microwave Digestion ofTungsten Ore (50% W; 10% Fe; 2% Mn)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHF

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 9 mL of HCl and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 230 25:00 20:00 800 900-1800 Off

Microwave Digestion ofTungsten Oxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Slowly add 2 mL of HNO₃, and 7 mL of HF. Gently swirl the mixture before closing the vessel.

1 220 20:00 20:00 800 900-1800 Off

Microwave Digestion ofTungsten Oxide

Microwave Digestion ofVermiculite

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”. The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureAdd 0.25 g of the sample into the digestion vessel. Add 1 mL of HNO₃, 4 mL of HCl, and 10 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofVermiculite

Microwave Digestion ofWollastonite (Calcium Silicate)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₃PO₄HNO₃HBF₄

ProcedureWeigh 0.1 g of sample into the digestion vessel. Add 3 mL of H₃PO₄, 2 mL of HNO₃, and 3 mL of HBF₄. HBF₄ should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing the vessel.

1 230 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofWollastonite (Calcium Silicate) in iPrep

Microwave Digestion ofZeolite

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

NotesAdd HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides. The above procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric HF Neutralization”

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofZeolite

Microwave Digestion ofZinc Oxide (ZnO)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 5 mL of HNO₃ and 2 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.25 g of the sample (onto a filter disk) into the digestion vessel. Add 5 mL HNO₃ and 2 mL HF. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 200 25:00 15:00 N/A 700-1800 Off

Microwave Digestion ofZinc Oxide (ZnO) in iPrep

Microwave Digestion ofZircon

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ProcedureAdd 50 mg of the sample into the digestion vessel. Add 4 mL of H₂SO₄, 1.5 mL of HNO₃, and 1.5 mL HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 270 40:00 30:00 N/A 700-1800 Off

Microwave Digestion ofZircon in iPrep

Industrial Hygiene

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃HF

NotesHF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

ProcedureWeigh 0.3 g of the sample into the digestion vessel. Add 6 mL of HCl, 3 mL of HNO₃, and 3 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFilter (MCE)

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃

Recommended Vessels20 mL MARSXpress

NotesThis method was completed in the 20 mL disposable liner.

ProcedureWeigh one 37mm filter and one 25 mm filter (60 mg total) into the digestion vessel. Add 1 mL of HCl and 1 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 20:00 10:00 N/A 1050-1800 Off

Microwave Digestion ofFilter (MCE)

Microwave Digestion ofFilter Paper - 37 mm Mixed Cellulose Ester

Industrial MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Filter (Approx 0.3 g)

ReagentsHNO₃

ProcedureWeigh 1 filter (approx 0.3 g) into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFilter Paper - 47 mm Polycarbonate

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFilter Paper - Cellulose

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂OHNO₃

NotesAdd H₂O before HNO₃. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 1 filter (approx 0.9 g) of the sample into the digestion vessel. Add 5 mL of H₂O, and 5 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 15:00 10:00 800 900-1800 Off

Microwave Digestion ofGhost Wipe

Industrial MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Wipe (Approx 2.5 g)

ReagentsHNO₃

NotesEnsure that the entire wipe is covered with reagent.

ProcedureWeigh 1 wipe (approx 2.5 g) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGhost Wipe

Microwave Digestion ofPaint Chips (For Pb Analysis)

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesHF may be required for a total dissolution.

1 200 15:00 10:00 800 900-1800 Off

ResultsSample was clear, colorless, with some remaining inorganic particles upon dilution to 50 mL.

Microwave Digestion ofQuartz Fiber Filters

Industrial MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.35 g (1 filter)

ReagentsHNO₃HF

Recommended VesselsMARSXpressMARSXpress Plus

NotesThis method should not exceed 180 degrees celsius.

This application should only be completed using the 75 mL Xpress or 110 mL Xpress Plus vessels. It is not suitable for the smaller Xpress vessels (55 mL, 20 mL, or 10 mL).

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization".

ProcedureWeigh 1 filter (approximately 0.35 g) of the sample into the digestion vessel. Add 8 mL of HNO₃ and 2 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 20:00 15:00 N/A 1030-1800 Off

ResultsSamples were clear, colorless, and particle free upon dilution to 50 mL with deionized water.

Microwave Digestion ofQuartz Fiber Filters

Microwave Digestion ofToray Filter

Industrial MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Filter (Approx 0.3 - 0.5 g)

ReagentsHNO₃

ProcedureWeigh 1 filter (approximately 0.3-0.5 g) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 240 20:00 20:00 N/A 700-1800 Off

Microwave Digestion ofToray Filter in iPrep

Microwave Digestion ofToray Filter (Anion)

Industrial MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Filter (Approx 0.3 - 0.5 g)

ReagentsHNO₃

ProcedureAdd 1 toray filter (Approx. 0.3 - 0.5 g) into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 30:00 800 900-1800 Off

Microwave Digestion ofToray Filter (Anion)

Microwave Digestion ofToray Filter (Cation)

Industrial MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Filter (Approx 0.3 -0.5 g)

ReagentsHNO₃

ProcedureAdd 1 toray filter (Approx. 0.3 - 0.5 g) into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 230 20:00 30:00 800 900-1800 Off

Microwave Digestion ofToray Filter (Cation)

Materials Science

Microwave Digestion ofAcrylic Tape

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃DI H₂OHClHF

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 3 mL of Deionized water, 7 mL of HNO₃, 1.5 mL of HCl, and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 30:00 10:00 N/A 700-1800 Off

Microwave Digestion ofAcrylic Tape in iPrep

GeoscienMARS 6

Method TypeClassic

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 9 mL HCl and 3 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 190 15:00 15:00 800 900-1800 Off

ResultsSample was clear, colorless, and particle free upon diltuion to 50 mL.

GeoscienMARS 6 (

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHCLHNO₃HF

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 6 mL of HCl, 2 mL of HNO₃, and 0.5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Allow any initial reaction to subside before sealing vessel.

1 230 25:00 15:00 N/A 700-1800 Off

GeoscienMARS 6 (

ReagentsSolid H₃BO₃DI H₂O

Heating Program

Method TypeClassic

Sample TypeStandard

1 170 25:00 10:00 N/A 700-1800 Off

Microwave Digestion ofCeramic Powder

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFBoric Acid (solid)H₂O

NotesThis procedure uses hydrofluoric acid. It is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed with boric acid.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 5 mL of HNO₃, 10 mL of HF, 4.4 g of Boric Acid solid, and 25 mL of H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 195 15:00 30:00 800 900-1800 Off

Microwave Digestion ofCeramic Powder

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFBoric Acid (solid)H₂O

NotesThis Application can only be run in the iPrep vessel.

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed with boric acid.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 5 mL of HNO₃, 10 mL of HF, 4.4 g of Boric Acid solid, and 25 mL of H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 195 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofCeramic Powder in iPrep

Microwave Digestion ofCircuit Board (Cryo - Ground)

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃

NotesHCl should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel. This method is considered a leach and may not provide a total digestion.

1 200 15:00 15:00 800 1030-1800 Off

ResultsSample was clear, with particles upon dilution to 50 mL.

Microwave Digestion ofCircuit Board (Cryo - Ground)

Microwave Digestion ofFerric Oxide

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃DI H₂O

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 10 mL of a 1:1:1 HCl, HNO₃, and Deionized H₂O mixture. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 25:00 20:00 N/A 700-1800 Off

Microwave Digestion ofFerric Oxide in iPrep

GeoscienMARS 6 (

Method TypeClassic

Sample TypeStandard

Heating Program

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 4 mL of H₂SO₄, 1.5 mL of HNO₃ and 1.5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Allow any initial reaction to subside before sealing vessel.

1 280 35:00 30:00 N/A 700-1800 High

GeoscienMARS 6 (

ReagentsH₃BO₃DI H₂O

Heating Program

Method TypeClassic

Sample TypeStandard

1 170 25:00 10:00 N/A 700-1800 High

Microwave Digestion ofGas Atomized Powder

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHF

1 200 30:00 20:00 N/A 700-1800 Off

ResultsSamples were clear, green in color and particle free upon dilution to 50 mL.

Microwave Digestion ofGas Atomized Powder in iPrep

Microwave Digestion ofGlass (Ground)

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHFHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGlass (Ground)

Microwave Digestion ofGraphite Composite - Cyanate Ester Resin

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1.5 g of the sample into the digestion vessel. Add 30 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 150 15:00 10:00 800 900-1800 Off

Microwave Digestion ofGraphite Composite - Cyanate Ester Resin

Microwave Digestion ofGraphite Composite - Epoxy Resin

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1.0 g of the sample into the digestion vessel. Add 30 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 150 10:00 10:00 800 900-1800 Off

Microwave Digestion ofGraphite Composite - Epoxy Resin

Microwave Digestion ofGraphite Composite - PEEK Resin

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 160 15:00 10:00 800 900-1800 Off

Microwave Digestion ofGraphite Composite - PEEK Resin

Microwave Digestion ofGraphite Fiber - Cyanate Siloxane Resin

Materials MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Laminate Square (Approx. 1 g)

ReagentsHNO₃

NotesSample must be completely covered with acid prior to digestion.

After digestion the samples are filtered and the fibers are rinsed with deionized water.

This method is for the gravimetric (weight) determination of fiber content.

ProcedureAdd 1 Laminate Square (Approx. 1 g) of the sample into the digestion vessel. Add 35 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 150 10:00 20:00 800 900-1800 Off

ResultsSample was digested, but the fiber filler material is left undigested for subsequent gravimetric analysis.

Microwave Digestion ofGraphite Fiber - Cyanate Siloxane Resin

Microwave Digestion ofGraphite Fiber - Epoxy Resin (Fiber Content)

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesSample must be completely covered with acid prior to digestion.

After digestion the samples are filtered and the graphite fibers are rinsed with deionized water.

This method is for the gravimetric (weight) determination of fiber content.

ProcedureWeigh 1 g of the sample into the digestion vessel. Add 30 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 150 10:00 10:00 800 900-1800 Off

ResultsSample was digested, but the fiber filler material is left undigested for subsequent gravimetric analysis.

Microwave Digestion ofGraphite Fiber - Epoxy Resin (Fiber Content)

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 210 25:00 25:00 N/A 700-1800 Off

GeoscienMARS 6 (

ProcedureAllow vessel to cool. Add 2.5 g H₃BO₃ and 25 mL of deionized H₂O into the vessel that contains the sample and acid.

ReagentsH₃BO₃ DI H₂O

Heating Program

Sample TypeOrganic

1 170 25:00 15:00 N/A 700-1800 Off

General Precautiona) If using HF, follow restrictions listed in HF Addendum.b) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.c) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.e) If programming as one touch, the ramp time and power will be automatically determined based on the number and type of vessels detected.

ResultsSample was clear and particle free upon dilution to 50 mL.

Microwave Digestion ofSiloxanes

Materials MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSiloxanes

Metals & Alloys

Microwave Digestion ofAlpha - Alumina

GeoscienMARS 6

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHCl

The maximum number of vessels that can be run for this method is 6.

ProcedureWeigh 0.5 g of sample into the digestion vessel. Add 10 mL HCl. Gently swirl the vessel to thoroughly mix the sample and acid.

1 240 30:00 59:59 N/A 900-1800 N/A

Microwave Digestion ofAlpha - Alumina in iPrep

Microwave Digestion ofAluminum (Metal)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHCl DI H₂O

NotesHCl should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HCl and 3 mL of DI H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAluminum (Metal)

Microwave Digestion ofAluminum Alloy

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClH₂O

NotesAdd H₂O before HCl. Add HCl slowly, and allow vessels to stand in the fume hood until initial reaction subsides.

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 5 mL of H₂O and 5 mL of HCl .Gently swirl the mixture before closing the vessel.

1 190 10:00 15:00 800 900-1800 Off

Microwave Digestion ofAluminum Alloy

Microwave Digestion ofAluminum Alloy (20% SiC)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHF

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization". The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 5 mL of HCl and 5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAluminum Alloy (20% SiC)

Microwave Digestion ofAluminum Alloy (BCS 182)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClH₂O₂ (30%)HF

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 20 mL of HCl, 10 mL of H₂O₂ (30%), and 5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

1 200 10:00 15:00 800 900-1800 Off

Microwave Digestion ofAluminum Alloy (BCS 182)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

NotesEasyPrep Plus requires a high temperature probe for this method.

ProcedureWeigh 0.25 g of sample into the digestion vessel. Add 6.5 mL H₃PO₄ and 3.5 mL H₂SO₄. Gently swirl the vessel to thoroughly mix the sample and acid.

1 270 30:00 20:00 N/A 900-1800 N/A

Microwave Digestion ofAluminum Oxide (H₃PO₄)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.1g

ReagentsH₃PO₄

This method may not provide a total digest of all samples. Hydrofluoric acid may be required to provide complete digestion of some sample matrixes.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 8 mL H₃PO₄. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 260 25:00 45:00 N/A 700-1800 Off

ResultsSample was clear and colorless with some white particles remaining upon dilution to 50 mL.

Microwave Digestion ofAluminum Oxide (H3PO4) in iPrep

Microwave Digestion ofBoron

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

1 175 15:00 25:00 800 900-1800 Off

Microwave Digestion ofBoron

Microwave Digestion ofBoron Carbide (B₄C)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃H₂SO₄HF

This procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid or other neutralization step should be used. This procedure can be found in the One Touch Method note entitled “BoricAcid HF Neutralization”.

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 5 mL of HNO₃ and 5 mL of H₂SO₄. Gently swirl to mix the sample particles in the acid. Slowly and carefully add 5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

Allow any initial reaction to subside before sealing vessel.

1 270 30:00 30:00 800 700-1800 Off

Microwave Digestion ofBoron Carbide (B4C) in iPrep

Microwave Digestion ofCarbonyl Iron

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClHBF₄

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 6 mL HNO₃, 2 mL HCl, and 1 mL HBF₄ slowly. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 30:00 20:00 N/A 700-1800 Off

ResultsSample was clear, yellow in color with no visible particles upon dilution to 50 mL.

Microwave Digestion ofCarbonyl Iron in iPrep

Microwave Digestion ofCobalt - Chromium Alloy (Co-Cr)

Metals anMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFDI H₂O

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization". Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

ProcedureAdd 0.5 g of the sample into the digestion vessel. Add 5 mL of HNO₃, 5 mL of HF, and 5 mL of deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCobalt - Chromium Alloy (Co-Cr) (Step 1 of 2)

Microwave Digestion ofCobalt - Chromium Alloy (Co-Cr)

Metals anMARS 6 (

ProcedureAllow vessels to cool. Open and add 4 mL of H₂O₂ (30%) dropwise. Wait approximately 15 minutes before closing the vessel.

ReagentsH₂O₂ (30%)

Heating Program

Sample TypeOrganic

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofCobalt - Chromium Alloy (Co-Cr) (Step 2 of 2)

Microwave Digestion ofCopper (Metal)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCopper (Metal)

Microwave Digestion ofCopper Ore

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃, HCl

NotesThis method is intended to be an acid leach, not a total digest. Hydrofluoric acid will be required to provide complete digestion of the sample matrix. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of Aqua Regia (3:1 HCl:HNO₃ ,). Gently swirl the mixture before closing the vessel.

1 180 15:00 10:00 800 900-1800 Off

ResultsSample was clear, and colorless with some remaining particles upon dilution to 50 mL.

Microwave Digestion ofCopper Ore

Microwave Digestion ofGallium

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 180 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGallium

Microwave Digestion ofIron - Chromium Alloy

Metals anMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHClHFDI H₂O

NotesThis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization". The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureAdd 0.5 g of the sample into the digestion vessel. Add 5 mL of HCl, 5 mL of HF, and 2 mL of deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofIron - Chromium Alloy (Step 1 of 2)

Metals anMARS 6 (

ProcedureAllow vessels to cool. Open and add 5 mL of HNO₃. Wait approximately 15 minutes before closing the vessel.

ReagentsHNO₃

Heating Program

Sample TypeOrganic

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofIron - Chromium Alloy (Step 2 of 2)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHFHNO₃

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 5 mL of HCl, 5 mL of HF, and 5 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 30:00 15:00 N/A 700-1800 Off

Microwave Digestion ofIron - Chromium Alloy in iPrep

Microwave Digestion ofIron - Lignosulfonate

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofIron - Lignosulfonate

Microwave Digestion ofIron - Manganese Alloy

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HCl and 2 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

1 200 15:00 20:00 800 900-1800 Off

Microwave Digestion ofIron - Manganese Alloy

Microwave Digestion ofIron - Silicon Alloy

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFHCl

Noteshis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HF, 5 mL HCl, and 3 mL HNO₃. Gently swirl the mixture and allow any initial reaction to subside before sealing vessel. HF should be added slowly and carefully to the sample.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofIron - Silicon Alloy

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 20:00 800 900-1800 Off

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 210 20:00 10:00 N/A 1030-1800 Off

ResultsThe samples were clear, green in color, and particle free upon dilution.

Microwave Digestion ofMagnesium Oxide (MgO 40 -54 %)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂O (deionized)HClHNO₃

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution.The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 5 mL of DI H₂O, 5 mL HCl, and 5 mL HNO₃. Gently swirl and allow approximately 15 minutes for pre-digestion.

1 200 20:00 20:00 800 900-1800 Off

Microwave Digestion ofMagnesium Oxide (MgO 40-54%)

GeoscienMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.2g of the sample into the digestion vessel. Add 10 mL HNO₃ and 3 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 180 15:00 10:00 800 900-1800 Off

GeoscienMARS 6 (

ProcedureAllow vessel to cool after completion of Step 1. Open and add 5 mL of HCl. Gently swirl the mixture and seal the vessel.

ReagentsHCl

Heating Program

Sample TypeOrganic

1 180 15:00 10:00 800 900-1800 Off

Microwave Digestion ofMetal Alloy - FeCr

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHFHNO₃

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 5 mL of HCl, 5 mL of HNO₃, and 5 mL of HF. Gently swirl the mixture before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMetal Alloy - FeCr

Microwave Digestion ofMetal Alloy - NiCr

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂OHNO₃ HF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 5 mL of H₂O, 5 mL of HNO₃, and 5 mL of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMetal Alloy - NiCr

Microwave Digestion ofMetal Alloy (Ag, Au, Cu)

Metals anMARS 6

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHClHNO₃

This method may not provide a total digest of all samples. Hydrofluoric acid may be required to provide complete digestion of some sample matrices.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 9 mL HCl, 1 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 220 25:00 25:00 N/A 900-1800 Off

ResultsSample was yellow in color, with some white particles remaining upon dilution to 50 mL.

Microwave Digestion ofMetal Alloy (Ag, Au, Cu) in iPrep

Microwave Digestion ofMetal Alloy (Ag, Co, Cu)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃Deionized H₂OHF

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 3 mL HNO₃, 3 mL Deionized H₂O and 3 mL HF (in this order). Add HF slowly and carefully. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 220 25:00 25:00 N/A 700-1800 Off

Microwave Digestion ofMetal Alloy (Ag, Co, Cu) in iPrep

Microwave Digestion ofMetal Alloy (Ag, Cu, Sn)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 3 mL HNO₃, 3 mL Deionized Water, and 3 mL HF in this order. Add HF slowly and carefully. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 220 25:00 25:00 N/A 700-1800 Off

Microwave Digestion ofMetal Alloy (Ag, Cu, Sn) in iPrep

Microwave Digestion ofMetal Ore - Precious

Metals anMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 10 mL of HNO₃, and 0.5 ml of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 20:00 800 900-1800 Off

Microwave Digestion ofMetal Ore - Precious (Step 1 of 2)

Microwave Digestion ofMetal Ore - Precious

Metals anMARS 6 (

ProcedureCool, vent and open vessel after step 1. Add 5 ml of HCl.

ReagentsHCl

Heating Program

Sample TypeOrganic

1 180 10:00 10:00 800 900-1800 Off

Microwave Digestion ofMetal Ore - Precious (Step 2 of 2)

Microwave Digestion ofMetals Alloy (Co-Cr)

Metals anMARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFH2O

NotesThe addition of Deionized Water may improve solubility of metal alloys.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 5 ml of HNO₃, 5 ml of HF, and 5 of ml H2O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMetals Alloy (Co-Cr) (Step 1 of 2)

Microwave Digestion ofMetals Alloy (Co-Cr)

Metals anMARS 6 (

ProcedureCool, vent and open vessel after step 1. Add 4 ml of H₂O₂, (30%)

ReagentsH₂O₂

Heating Program

Sample TypeOrganic

1 200 10:00 10:00 800 900-1800 Off

General Precautiona) This procedure is a reference point for sample digestion using a CEM system and may need to be modified or changed to obtain the required results on your sample.b) If using a vessel other than the recommended choice, adjust sample size and pressure limit to values appropriate for the vessel chosen.c) The control / reference vessel must contain the largest and most reactive sample.d) Manual venting of CEM vessels should be performed when wearing hand/eye/body protection and when the vessel contents are at or below room temperature to avoid the potential for chemical burns. Always point the vent hole away from the operator.

Microwave Digestion ofMetals Alloy (Co-Cr) (Step 2 of 2)

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 210 20:00 10:00 N/A 1030-1800 Off

ResultsThe samples were clear, light green in color, and particle free upon dilution.

Microwave Digestion ofNickel (Metal)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 0.5 g of sample into the digestion vessel. Add 5 mL of HNO₃ and 5 mL of DI H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofNickel (Metal)

Microwave Digestion ofNiobium - Titanium Alloy

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHFHNO₃DI H₂O

Noteshis procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”. The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 1 mL of HF, 5 mL HNO₃, and 5 mL of deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofNiobium - Titanium Alloy

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂O₂ (30%)HClHNO₃

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 5 mL of H₂O₂ (30%), 5 mL HCl dropwise (wait for reaction to subside before adding next acid), and 5 mL HNO₃. Gently swirl the mixture and wait approximately 1 hour before closing the vessel.

1 210 20:00 20:00 800 900-1800 Off

Microwave Digestion ofPlatinum Metal

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of Aqua Regia (3:1 HCl:HNO₃). Gently swirl the mixture before closing the vessel.

1 180 15:00 20:00 800 900-1800 Off

Microwave Digestion ofPlatinum Metal

Microwave Digestion ofPlatinum Ruthenium

Metals anMARS 6

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHClHNO₃

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 6 mL of HCl, and 2 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 240 30:00 59:59 N/A 900-1800 Off

Microwave Digestion ofPlatinum Ruthenium in iPrep

Microwave Digestion ofRhodium Sponge

Metals anMARS 6

Sample TypeHCl

Heating Program

ReagentsHClH₂O₂

The maximum number of vessels that can be run with this method is 6.

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 10 mL of HCl and 2 mL of H₂O₂ dropwise. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 250 25:00 45:00 N/A 900-1800 Off

Microwave Digestion ofRhodium Sponge in iPrep

Microwave Digestion ofSelenium Alloy

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1 g (0.5 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSelenium Alloy

Microwave Digestion ofSn - Ag Solder

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂OHNO₃HF

NotesCaution must be taken when adding the acid solution to the sample as the reaction is very vigorous and exothermic.

Allow any initial reaction to subside before sealing the vessel.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Slowly add dropwise 10 ml of 1:1:1 H₂O:HNO₃:HF (premixed) to the sample. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 15:00 10:00 800 900-1800 Off

Microwave Digestion ofSn - Ag Solder

Microwave Digestion ofStainless and Low Carbon Steel

Metals anMARS 6

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHNO₃HCl

ProcedureAdd 0.25 g of the sample into the digestion vessel. Add 3 mL of HNO₃, and 9 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 240 30:00 25:00 N/A 700-1800 Off

ResultsSample was clear, gold colored, and particle free upon dilution to 50 mL.

Microwave Digestion ofStainless and Low Carbon Steel in iPrep

Microwave Digestion ofStainless Steel

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFHCl

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 9 ml of HCl, 3 mL of HNO₃, and 3 mL of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofStainless Steel

Microwave Digestion ofSteel (Stainless)

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHNO₃

NotesThis method may not provide a total digest of all samples. Hydrofluoric acid will be required to provide complete digestion of some sample matrixes.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSteel (Stainless)

Microwave Digestion ofStrontium Ferrite

Metals anMARS 6

Method TypeClassic

Sample TypeHCl

Heating Program

ReagentsHClHF

The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution.

ProcedureWeigh 0.5 g of sample into the digestion vessel. Add 10 mL of HCl and 0.5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 30:00 15:00 N/A 900-1800 Off

Microwave Digestion ofStrontium Ferrite in iPrep

Microwave Digestion ofTitanium Alloy

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFHCl

1 200 15:00 30:00 800 900-1800 Off

Microwave Digestion ofTitanium Alloy

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 210 15:00 15:00 800 900-1800 Off

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHClHF

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 9 mL of HCl and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 230 25:00 20:00 800 900-1800 Off

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 5 mL of HNO₃ and 2 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.HF should be added slowly and carefully to the sample. Allow any initial reaction to subside before sealing vessel.

1 200 15:00 15:00 800 900-1800 Off

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.25 g of the sample (onto a filter disk) into the digestion vessel. Add 5 mL HNO₃ and 2 mL HF. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 200 25:00 15:00 N/A 700-1800 Off

Microwave Digestion ofZinc Oxide (ZnO) in iPrep

Microwave Digestion ofZircon Alloy

Metals anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHFHNO₃DI H₂O

ProcedureAdd 1.0 g of the sample into the digestion vessel. Add 5 mL of HF, 1 mL HNO₃, and 10 mL of deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofZircon Alloy

Nutraceuticals

Microwave Digestion ofBiotin

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO₃, and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 Off

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃, or alternatively 9 mL HNO₃, and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 20:00 10:00 800 900-1800 Off

Microwave Digestion ofChili Powder

Food TestMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight2 g

ProcedureWeigh 2 g of the sample into the digestion vessel. Add 10 mL HNO₃ and 5 mL deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 240 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofChili Powder in iPrep

Food TestMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 6 mL of HNO₃, 3 mL of deionized H₂O, and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGinkgo (Ground)

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGinkgo (Whole Pill)

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Pill (Approx 1.0 g)

ProcedureWeigh 1 pill (approx 1.0 g) into the digestion vessel. Add 10 mL of HNO₃ and 2 mL of H₂O₂. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureTransfer 2 mL of of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 210 30:00 20:00 N/A 700-1800 Off

Results Sample was clear, colorless and particle free upon dilution to 50 mL

Microwave Digestion ofInfant Formula (Liquid) in iPrep

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureTransfer 1 mL (0.5 mL w/Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 210 20:00 20:00 N/A 700-1800 Off

Microwave Digestion ofInfant Formula (Powder) in iPrep

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofKelp

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight 0.5 g

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 20:00 15:00 800 900-1800 Off

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 off

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

AgriculturMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Food TestMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Organic Chemicals

Microwave Digestion ofDi-(2-ethylhexyl)phosphoric Acid

Organic CMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureTransfer 0.5 mL of the sample into the digestion vessel. Add 10 mL HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 220 30:00 15:00 N/A 700-1800 Off

Microwave Digestion ofDi-(2-ethylhexyl)phosphoric Acid in iPrep

Microwave Digestion ofDiazo Sulfonyl Chloride

Organic CMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofDiazo Sulfonyl Chloride

Microwave Digestion ofFood Coloring (Erythrosine Based)

Organic CMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFood Coloring (Erythrosine Based)

Microwave Digestion ofMethylcellulose (Hydroxypropyl)

Organic CMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 10 mL of HNO₃ and 2 mL of deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMethylcellulose (Hydroxypropyl)

Microwave Digestion ofMonoethanolamine

Organic CMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMonoethanolamine

Microwave Digestion ofPhotoresist

Organic CMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolygluteramide

Organic CMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 220 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolygluteramide

Paints & Coatings

Microwave Digestion ofPaint (Latex Based Liquid)

Paints anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesHF is required for a total dissolution of inorganic filler if present.

If this procedure uses hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled “Boric Acid HF Neutralization”.

ProcedureWeigh 1 g (0.5 g w/Xpress Vessels) of sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 200 15:00 10:00 800 900-1800 Off

Microwave Digestion ofPaint (Latex Based Liquid)

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesHF may be required for a total dissolution.

1 200 15:00 10:00 800 900-1800 Off

ResultsSample was clear, colorless, with some remaining inorganic particles upon dilution to 50 mL.

Organic CMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolyurethane

Paints anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Paints anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 6 mL HNO₃, and 3 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 230 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofPolyurethane in iPrep

Microwave Digestion ofToner (Ink)

Paints anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis application can only be run in the iPrep vessel. The use of HF may be required to digest any silicates found in the sample.

1 240 20:00 20:00 N/A 700-1800 Off

ResultsSample was clear, colorless with some particles remaining upon dilution to 50 mL.

Microwave Digestion ofToner (Ink) in iPrep

Microwave Digestion ofToner (Powder)

Paints anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 240 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofToner (Powder) in iPrep

Paper & Pulp

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Industrial MARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂OHNO₃

NotesAdd H₂O before HNO₃. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

ProcedureWeigh 1 filter (approx 0.9 g) of the sample into the digestion vessel. Add 5 mL of H₂O, and 5 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 15:00 10:00 800 900-1800 Off

Microwave Digestion ofPaper Pulp

Paper anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPaper Pulp

Personal Care

AgriculturMARS 6

Sample TypeOrganic

Heating Program

1 170 15:00 15:00 800 900-1800 Off

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 8 mL of HNO₃ and 2 mL of HF. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

1 210 15:00 15:00 800 900-1800 Off

ConsumeMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Pharmaceutical & Biotech

Microwave Digestion ofAllergy Pill (Ground)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAllergy Pill (Ground)

Microwave Digestion ofAllergy Pill (Whole Pill)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 1 pill (Approx 1.0 g) into the digestion vessel. Add 10 mL of HNO₃ and 2 mL of H₂O₂ (30%). Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAllergy Pill (Whole Pill)

Microwave Digestion ofAntioxidant (Ground)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAntioxidant (Ground)

Microwave Digestion ofAntioxidant (Whole Pill)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 1 pill (approx 1.4 g) into the digestion vessel. Add 10 mL of HNO₃ and 2 mL of H₂O₂ (30%). Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAntioxidant (Whole Pill)

Microwave Digestion ofAPIs with Aromatic Ring Structures

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 9 mL of HNO₃ and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 250 30:00 25:00 N/A 700-1800 N/A

ResultsSample was clear, colorless, and particle free upon dilution to 50 mL. If the sample contains precious metals the diluted sample may be the color associated with the metals.

Microwave Digestion ofAPIs with Aromatic Ring Structures in iPrep

Microwave Digestion ofAscorbic Acid

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofAscorbic Acid

Microwave Digestion ofAspirin (Ground)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAspirin (Ground)

Microwave Digestion ofAspirin (Whole)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight 1 pill (Approx 1.0 g)

ReagentsHNO₃

ProcedureWeigh 1 pill (Approx 1.0 g) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAspirin (Whole)

Microwave Digestion ofBeta Carotene

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofBeta Carotene

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofCalcium Carbonate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

NotesThe addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis. Allow initial reaction to subside before sealing vessel.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Slowly add 9 mL of HNO₃, and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofCalcium Carbonate

Microwave Digestion ofCalpan Pantothenic Acid

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCalpan Pantothenic Acid

Microwave Digestion ofCephalexin

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCephalexin

Microwave Digestion ofChromium Chelate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Resultsample was clear, colorless, and particle free upon dilution to 50 mL.

Microwave Digestion ofChromium Chelate

Microwave Digestion ofCold and Flu Medicine (Liquid)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureTransfer 0.75 mL of the sample into the digestion vessel. Add 9 mL of HNO₃ and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCold & Flu Medicine (Liquid)

Microwave Digestion ofCupric Sulfate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

ResultsSample was clear, blue in color, and particle free upon dilution to 50 mL.

Microwave Digestion ofCupric Sulfate

Microwave Digestion ofDiclofenac K

PharmaceMARS 6

Sample TypeOrganic

Heating Program

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofDiclofenac K

Microwave Digestion ofEmpty Capsule (Gel)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight1 Capsule (Approx 0.1 g)

ReagentsHNO₃

ProcedureWeigh 1 capsule (Approx 0.1 g) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofEmpty Capsule (Gel)

Microwave Digestion ofErythromycin

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 9 mL of HNO₃ and 1 mL HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofErythromycin

Microwave Digestion ofFish Oil (No Capsule)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFish Oil (No Capsule)

Microwave Digestion ofFlurbiprofen

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFlurbiprofen

Microwave Digestion ofFolic Acid

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFolic Acid

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 1 pill (approx 1.0 g) into the digestion vessel. Add 10 mL of HNO₃ and 2 mL of H₂O₂. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofHydrocodone

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 220 25:00 15:00 800 900-1800 Off

Microwave Digestion ofHydrocodone

Microwave Digestion ofIbuprofen (Ground)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.3 g of the sample into the digestion vessel. Add 8 mL HNO₃ and 2 mL HF. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 200 20:00 15:00 800 1030-1800 Off

Microwave Digestion ofIbuprofen (Ground)

NutraceutiMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofLosartan K

PharmaceMARS 6

Sample TypeOrganic

Heating Program

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofLosartan K

Microwave Digestion ofMagnesium Carbonate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

NotesAllow any reaction to subside before sealing the vessel. The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofMagnesium Carbonate

Microwave Digestion ofMagnesium Oxide

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMagnesium Oxide

Microwave Digestion ofManganese Carbonate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofManganese Carbonate

Microwave Digestion ofMedroxyprogesterone

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMedroxyprogesterone

Microwave Digestion ofMetformin (Ground)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMetformin (Ground)

Microwave Digestion ofMineral Oil

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMultivitamin (Ground)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMultivitamin (Ground)

Microwave Digestion ofMultivitamin (Whole)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureWeigh 1 pill (Approx 2.5 g) into the digestion vessel. Add 10 mL of HNO₃ and 2 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMultivitamin (Whole)

Microwave Digestion ofNaproxen Sodium

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 220 25:00 15:00 800 900-1800 Off

Microwave Digestion ofNaproxen Sodium

Microwave Digestion ofNiacinamide

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofNiacinamide

Microwave Digestion ofPantothenic Acid

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofPantothenic Acid

Microwave Digestion ofPotassium Chelate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofPotassium Chelate

Microwave Digestion ofProtopic Ointment

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 220 25:00 15:00 800 900-1800 Off

Microwave Digestion ofProtopic Ointment

Microwave Digestion ofPyridoxine

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

Recommended Equipment

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofPyridoxine

Microwave Digestion ofRiboflavin

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 230 20:00 30:00 800 900-1800 Off

Microwave Digestion ofRiboflavin

Microwave Digestion ofSelenium Chelate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofSelenium Chelate

Microwave Digestion ofSleep Aid

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 220 25:00 15:00 800 900-1800 Off

Microwave Digestion ofSleep Aid

Microwave Digestion ofSodium Polystyrene Sulfonate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsDI H₂OHNO₃

ProcedureWeigh 0.3 g of the sample into the digestion vessel. Add 6 mL of HNO₃ and 2 mL of deionized H₂O. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 250 25:00 25:00 N/A 700-1800 Off

Microwave Digestion ofSodium Polystyrene Sulfonate in iPrep

Microwave Digestion ofStearic Acid

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofStearic Acid

Microwave Digestion ofSudan Orange (4-Fluorophenyl)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 9 mL of HNO₃ and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 250 25:00 25:00 N/A 700-1800 Off

Microwave Digestion ofSudan Orange (4-Fluorophenyl) in iPrep

Microwave Digestion ofThiamine

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofThiamine

Microwave Digestion ofTransdermal Patch

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HClNH₄F

NotesThis procedure uses ammonium fluoride, which creates in-situ hydrofluoric acid. If it is necessary to complex the residual hydrofluoric acid or redissolve insoluble fluorides formed, an additional complexation step with boric acid should be used. This procedure can be found in the One Touch Method note entitled "Boric Acid HF Neutralization".

ProcedureWeigh 0.1 g of the sample into the digestion vessel. Add 9 mL of HNO₃, 1 mL of HCl, and 0.1 g of NH₄F. Gently swirtl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 20:00 30:00 800 900-1800 Off

ResultsCompleted samples were clear, colorless and particle free upon dilution to 50 mL.

Microwave Digestion ofTransdermal Patch

Microwave Digestion ofTrixie Phosphate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureWeigh 50 mg of the sample into the digestion vessel. Add 6 mL of HNO₃ and 1 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 250 30:00 25:00 N/A 700-1800 Off

Microwave Digestion ofTrixie Phosphate in iPrep

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofVitamin B-12

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofVitamin B-12

Microwave Digestion ofVitamin C (Ground)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofVitamin C (Ground)

Microwave Digestion ofVitamin C (Whole)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 1 pill (Approx 1.4 g) into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofVitamin C (Whole)

Microwave Digestion ofVitamin Concentrate (High Fe)

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO3HCl

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 5 mL of HNO3 and 5 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 180 15:00 10:00 800 900-1800 Off

Microwave Digestion ofVitamin Concentrate (High Fe)

Microwave Digestion ofVitamin D

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 210 20:00 15:00 800 900-1800 Off

Microwave Digestion ofVitamin D

Microwave Digestion ofVitamin D3

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofVitamin D3

Microwave Digestion ofVitamin E

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HCl

1 230 20:00 30:00 800 900-1800 Off

Microwave Digestion ofVitamin E

Microwave Digestion ofVitamins

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HFHCl

ProcedureWeigh 0.5 g (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 9 mL of HNO₃, 1 mL HCl, and 1 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 15:00 30:00 800 900-1800 Off

Microwave Digestion ofVitamins

Microwave Digestion ofZinc Sulfate

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofZinc Sulfate

Plastics, Polymers, & Oils

Microwave Digestion ofAcrylamide

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofAcrylamide

Microwave Digestion ofBrominated Flame Retardant

Plastics, MARS 6

Method TypeClassic

Sample TypeStandard

Heating Program

ReagentsH₂SO₄HNO₃H₂O₂

Stirring is required for complete dissolution of this sample type.

ProcedureWeigh 50 mg of the sample into the digestion vessel. Insert stir bar into the vessel. Add 4.5 mL H₂SO₄ and 4.0 mL HNO₃ to the vessel, then add 1.5 mL H₂O₂ dropwise. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 270 30:00 45:00 N/A 900-1800 Med

ResultsSample was clear and colorless, and particle free upon dilution to 50 mL.

Microwave Digestion ofBrominated Flame Retardant in iPrep

Microwave Digestion ofBunker Oil

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis application can only be run in the iPrep vessel. The pre-digestion step will allow the lighter fuel fractions to react before sealing the vessels.

1 240 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofBunker Oil in iPrep

GeoscienMARS 6

Sample TypeOrganic

Heating Program

Max Sample WeightVaries by sample

ReagentsH₂SO₄

NotesEasyPrep Plus requires a high temperature probe for this method. This method is for the pretreatment of large sample sizes or difficult organic samples that are resistant to oxidation. After the char is complete, the vessel is opened and a normal oxidation with HNO₃ can be run, usually at around 200C.

ProcedureAdd 6 mL H₂SO₄ into the vessel that contains the sample and acid.

1 260 15:00 30:00 800 900-1800 Off

Microwave Digestion ofCrude Oil

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofCrude Oil

Microwave Digestion ofDiesel Fuel

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe pre-digestion step will allow the lighter fuel fractions to react before sealing the vessels

ProcedureWeigh 0.25 g of the sample into the digestion vessel. Add 10 mL of HNO₃ . Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofDiesel Fuel

Microwave Digestion ofEpoxy Hardener

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofEpoxy Hardener

Microwave Digestion ofFatty Alcohol

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofFatty Alcohol

Microwave Digestion ofFluorinated Ethylene Propylene (FEP)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsH₂SO₄HNO₃H₂O₂

ProcedureWeigh 120 mg of the sample into the digestion vessel. Add 4.5 mL of H₂SO₄, 4 mL of HNO₃, and 1.5 mL of H₂O₂ slowly. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 280 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofFluorinated Ethylene Propylene (FEP) in iPrep

Microwave Digestion ofFuel Oil (Slurry)

Plastics, MARS 6 (

Sample TypeOrganic

Heating Program

ReagentsHNO₃ HF

1 250 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofFuel Oil (Slurry) in iPrep (Step 1 of 2)

Microwave Digestion ofFuel Oil (Slurry)

Plastics, MARS 6 (

ProcedureAllow vessels to cool. Open and add 2 mL H₂O₂ (30%) dropwise. Wait approximately 15 minutes before closing the vessel.

ReagentsH₂O₂ (30%)

Heating Program

Sample TypeOrganic

1 210 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofFuel Oil (Slurry) in iPrep (Step 2 of 2)

Microwave Digestion ofGasoline

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureAdd 0.1 g of the sample into the digestion vessel. Slowly add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofGasoline

Microwave Digestion ofGlycerol Ester of Wood Rosin (Ground)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g (ground) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to predigest before closing the vessel.

1 220 30:00 30:00 N/A 700-1800 Off

ResultsSample was clear, colorless, and particle free upon dilution to 50 mL with deionized water.

Microwave Digestion ofGlycerol Ester of Wood Rosin (Ground) in iPrep

Microwave Digestion ofHDPE

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofHDPE

GeoscienMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofKevlar

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis application can only be run in the iPrep vessel. Ensure that all fibers are completely covered with acid and not adhered to the vessel liner wall.

1 250 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofKevlar in iPrep

Microwave Digestion ofLube Oil

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofLube Oil

Microwave Digestion ofMethyl Ester of Hydrogenated Wood Rosin (Ground)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃ . Gently swirl the mixture and wait approximately 15 minutes to predigest before closing the vessel.

1 220 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofMethyl Ester of Hydrogenated Wood Rosin (Ground) in iPrep

PharmaceMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMotor Oil (New)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMotor Oil (New)

Microwave Digestion ofMotor Oil (Waste)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.25 g (0.1 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to pre-digest before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofMotor Oil (Waste)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis application can only be run in the iPrep vessel. The pre-digestion step will allow the lighter fuel fractions to react before sealing the vessels.

1 240 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofMotor Oil (Waste) in iPrep

Microwave Digestion ofNylon

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofNylon

Microwave Digestion ofOil Wear Metals

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofOil Wear Metals

Microwave Digestion ofPET (Polyethylene Terephthalate)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 8 mL of HNO₃ and 2 mL of H₂SO₄. Gently swirl the mixture before closing the vessel.

1 230 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPET (Polyethylene Terephthalate)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 250 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofPET (Polyethylene Terephthalate) in iPrep

Microwave Digestion ofPetrolatum

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPetrolatum

Microwave Digestion ofPlasticizer (Filter)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 230 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofPlasticizer (Filter) in iPrep

Microwave Digestion ofPlasticizers

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

The addition of Conc. HCl (0-4 mL) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution.The amount of HCl will vary depending on the matrix and the concentration of the analytes.

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 6 mL HNO₃ and 3 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 230 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofPlasticizers in iPrep

Microwave Digestion ofPolycarbonate Resin

Plastics, MARS 6 (

Sample TypeOrganic

Heating Program

ReagentsH₂SO₄

NotesEasy Prep Plus requires a high temperature probe for this method.

ProcedureChar Step Weigh 1.0 g of the sample into the digestion vessel. Add 6 mL of H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 260 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolycarbonate Resin (Step 1 of 2)

Microwave Digestion ofPolycarbonate Resin

Plastics, MARS 6 (

ProcedureProceeding step one, add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

ReagentsHNO₃

Heating Program

Sample TypeOrganic

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolycarbonate Resin (Step 2 of 2)

Microwave Digestion ofPolyethersulfone (Filter Paper)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 1 filter paper (Approx 0.1 g) into the digestion vessel. Add 8 mL of HNO₃ and 2 mL H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 230 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolyethersulfone (Filter Paper)

Microwave Digestion ofPolyethylene - High Density

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.25 g (0.1 g w/Xpress Vessels) of the sample into the digestion vessel. Add 10 ml of HNO₃. Gently swirl the mixture before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolyethylene - High Density

Microwave Digestion ofPolyphenylene Sulfide

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolyphenylene Sulfide

Microwave Digestion ofPolypropylene

Plastics, MARS 6

Sample TypeOrganic

Heating Program

Max Sample Weight0.25 g (0.1 g w/Xpress)

ReagentsHNO₃

ProcedureWeigh 0.25 (0.1 g w/Xpress vessels) of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPolypropylene

Microwave Digestion ofPolyterpene Resin

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThis application can only be run in an iPrep.

ProcedureWeigh 0.5 g of the sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture and wait approximately 15 minutes to predigest before closing the vessel.

1 220 30:00 30:00 N/A 700-1800 Off

Microwave Digestion ofPolyterpene Resin in iPrep

Paints anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Paints anMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

1 230 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofPolyurethane in iPrep

Microwave Digestion ofPVC (Polyvinyl Chloride Resin)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

ProcedureWeigh 0.25 g (0.1 g w/Xpress Vessels) of sample into the digestion vessel. Add 10 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofPVC (Polyvinyl Chloride Resin)

Microwave Digestion ofRubber

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 220 15:00 15:00 800 900-1800 Off

Microwave Digestion ofRubber

Microwave Digestion ofRubber (Synthetic)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 210 15:00 15:00 800 900-1800 Off

Microwave Digestion ofRubber (Synthetic)

Microwave Digestion ofSilane Terminated Polyether

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HCl

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 6 mL HNO₃ and 3 mL of HCl. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 230 30:00 20:00 N/A 700-1800 Off

Microwave Digestion ofSilane Terminated Polyether in iPrep

Microwave Digestion ofSilicone Tubing - TiO₂ Filler

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃HF

ProcedureWeigh 0.2 g of the sample into the digestion vessel. Add 2 mL HNO₃ and 5 mL of HF. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel. Add HF slowly, and allow vessels to stand in the fume hood until the initial reaction subsides.

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofSilicone Tubing - TiO2 Filler

Microwave Digestion ofStyrene Acrylate

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofStyrene Acrylate

Microwave Digestion ofTerephthalic Acid

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 230 15:00 15:00 800 900-1800 Off

Microwave Digestion ofTerephthalic Acid

Microwave Digestion ofWax (Petroleum Based)

Plastics, MARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

1 200 15:00 15:00 800 900-1800 Off

Microwave Digestion ofWax (Petroleum Based)

Water & Wastewater Treatment

EnvironmMARS 6

Sample TypeWater

Heating Program

ReagentsHNO₃ HCl

Recommended Vessels75 mL MARSXpressEasyPrepEasyPrep PlusMARSXpress Plus

ProcedureTransfer 50 mL of the sample into the digestion vessel. Add 3 mL of HNO₃, and 2 mL HCl. Gently swirl the mixture before closing the vessel.

1 165 30:00 0:00 800 900-1800 Off

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ReagentsHNO₃

NotesThe use of HF may be required to digest any silicates found in the sample. The addition of Conc. HCl (0-4 ml) is appropriate for the stabilization of Ag, Ba and Sb, and high concentrations of Fe and Al in solution. The amount of HCl will vary depending on the matrix and the concentration of the analytes. The addition of HCl may, however, limit the techniques or increase the difficulties of analysis.

1 200 15:00 15:00 800 900-1800 Off

EnvironmMARS 6

Sample TypeWater

Heating Program

ReagentsHNO₃

ProcedureTransfer 25 mL of the sample into the digestion vessel. Add 5 mL of HNO₃. Gently swirl the mixture before closing the vessel. If a high organic content is suspected, a 10 ml sample volume is recommended.

1 170 10:00 10:00 800 900-1800 Off

EnvironmMARS 6

Sample TypeWater

Heating Program

ReagentsHNO₃

ProcedureTransfer 45 mL of the sample into the digestion vessel. Add 5 mL of HNO₃. Gently swirl the mixture before closing the vessel.

1 170 10:00 10:00 800 900-1800 Off

EnvironmMARS 6

Sample TypeWater

Heating Program

ProcedureTransfer 45 mL of the sample into the digestion vessel. Add 5 mL of HNO3, or alternatively 4 mL of HNO₃ and 1 mL HCl. Gently swirl the mixture before closing the vessel.

1 170 10:00 10:00 800 900-1800 Off

EnvironmMARS 6

Sample TypeOrganic

Heating Program

ProcedureWeigh 0.5 g (dry weight) (0.25 g w/Xpress Vessels) of the sample into the digestion vessel. Add 5 mL of HNO₃, and 5 mL of H₂O₂. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 200 15:00 15:00 800 900-1800 Off

EnvironmMARS 6

Method TypeClassic

Sample TypeWater

Heating Program

ReagentsK₂S₂O₈ H₂SO₄

Recommended EquipmentMARS 6

ProcedureTransfer 50 mL of H₂O into the digestion vessel. Add 0.5 g of K₂S₂O₈ and 1 mL of H₂SO₄. Gently swirl the mixture and wait approximately 15 minutes before closing the vessel.

1 170 15:00 15:00 800 900-1800 Off

Microwave Acid Digestion Method Note Compendium€¦ · Compendium October 1, 2019. Contents...

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