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Methods in Enzymology
Volume 122
Vitamins and Coenzymes
Part G
Donald B . McCormickFrank Chytil
CONTRIBUTORS TO VOLUME 122 xi
PREFACE XVii
VOLUMES IN SERIES X1X
Section I . Ascorbic Acid
1 . High-Performance Liquid Chromatographic Sepa- LANDIS W . HONER AN Dration of Ascorbic Acid, Erythorbic Acid, De- KEVIN B . Hicks
3hydroascorbic Acid, Dehydroerythorbic Acid ,Diketogulonic Acid, and Diketogluconic Acid
2. Glutathione Dehydrogenase (Ascorbate)
ROBERT L . STAHL ,
LEONARD F . LIEBES, AN D
ROBERT SILBER
1 0
Section II . Thiamin: Phosphates and Analog s
3 . Determination of Thiamin and Its Phosphate TAKASHI KAWASAKI
1 5
Esters by High-Performance Liquid Chroma-tography
4, Separation and Determination of Thiamin and Its JACK R . COOPER AN D
Phosphate Esters by SP-Sephadex Chromatog- TosHlo MATSUDA
20
raphy
5. Enzymatic Synthesis of Thiamin Triphosphate
JACK R. COOPER AN D
KOHSUKE NISHINO
24
Section III . Pantothenic Acid, Coenzyme A, and Derivative s
6. Pantothenase-Based Assay of Pantothenic Acid R . KALERVO AURAS
3 3
7. Enzymatic Hydrolysis of Pantetheine
CARL T . WITTWER ,
BONITA W. WYSE, AND
R . GAURTH HANSEN
3 6
8. Micromethod for the Measurement of Acetyl ARTHUR G . HUNT
4 3Phosphate and Acetyl Coenzyme A
9. Preparation of Radioactive Acyl Coenzyme A
AMIYA K . HAJRA AN DJAMES E . BISHOP
5 0
10 . Purification of Plant Acetyl-CoA :Acyl Carrier TAKASHI SHIMAKATA AN DProtein Transacylase
PAUL K . STUMPF
5 3
Section IV. Biotin and Derivative s
11 . Separation of Biotin and Analogs by High-Perfor- DELORES M . BOWERS-KOMRO ,mance Liquid Chromatography
JANE L . CHASTAIN, AN DDONALD B . MCCORMICK
63
12. Fluorometric Assays for Avidin and Biotin
R . D . NARGESSI AN DD . S . SMITH
67
13 . Solid-Phase Assay for d-Biotin and Avidin on L . GOLDSTEIN ,Cellulose Disks
S . A . YANKOFSKY ,AND G . COHEN
7 2
14 . Purification of Avidin and Its Derivatives on 2- GEORGE A . ORR ,Iminobiotin-6-aminohexyl-Sepharose 4B
GAYLE C . HENEY, ANDRON ZEHEB
8 3
15 . Use of Avidin-Iminobiotin Complexes for Purify- RON ZEHEB AN Ding Plasma Membrane Proteins
GEORGE A . ORR
8 7
Section V . Pyridoxine, Pyridoxamine, Pyridoxal :Analogs and Derivatives
16 . Preparation of [ 14 C]Pyridoxal 5'-Phosphate
ALEC LUI ,LAWRENCE LUMENG, AN DTING-KAI LI
97
l7 . Cation-Exchange High-Performance Liquid Chro- STEPHEN P . COBURN ANDmatographic Analysis of Vitamin B 6
J . DENNIS MAHUREN
102
18 . Highly Sensitive Methods for Assaying the En- ALFRED H . MERRILL, JR .zymes of Vitamin B 6 Metabolism
AND ELAINE WANG
11 0
19 . Modified Purification of Pyridoxamine (Pyridox- DELORES M . BOWERS-KOMRO ,ine) 5'-Phosphate Oxidase from Rabbit Liver by ToRY M . HAGEN, AN D5'-Phosphopyridoxyl Affinity Chromatography DONALD B . MCCORMICK
11 6
20. Monoclonal Antibodies to Vitamin B 6
JASON M . KITTLER ,DACE VICEPS-MADORE .JOHN A . CIDLOWSKI ,NATALIE T . MEISLER, ANDJOHN W . THANASSI
120
21 . Pyruvoyl-Dependent Histidine Decarboxylase ESMOND E . SNELL
12 8from Lactobacillus 30a : Purification and Prop-erties
22 . Prohistidine Decarboxylase from Lactobacillus ESMOND E . SNELL AN D30a
QUANG KHAI HUYNH
13 5
23 . Pyridoxal Phosphate-Dependent Histidine Decar- ESMOND E . SNELL AN Dboxylase from Morganella AM-15
BEVERLY M . GUIRARD
13 9
Section VI . Nicotinic Acid : Analogs and Coenzymes
24. Simultaneous Extraction and Combined Biolumi- MATTI T . KARP ANDnescent Assay of Oxidized and Reduced PAULI I . VUORINEN
14 7Nicotinamide Adenine Dinucleotid e
25 . Purification of Reduced Nicotinamide Adenine Di- DEXTER B . NORTHROP ,nucleotide by Ion-Exchange and High-Perfor- COLIN J . NEWTON, AN Dmance Liquid Chromatography
STEVEN M . FAYNOR
152
26 . Desalting of Nucleotides by Reverse-Phase High- DEXTER B . NORTHROP ANDPerformance Liquid Chromatography
COLIN J . NEWTON
155
27 . Preparation of 3-Aminopyridine 1,N 6-Ethe- BRUCE M . ANDERSON AN Dnoadenine Dinucleotide and 3-Aminopyridine CONSTANCE D . ANDERSON
15 61,N6-Ethenoadenine Dinucleotide Phosphate
28 . Simultaneous Photometric Determination of Oxy- MANASE M . DAMOREANUgen Consumption and NAD(P)H Formation or AND MARIUS I . TELIA
16 1Disappearance Using a Continuous Flow Cu -vett e
29 . Spectrophotometric Assay of NADase-Catalyzed BRUCE M . ANDERSON ANDReactions
DAVID A . YOST
169
30 . Snake Venom NAD Glycohydrolase : Purification, BRUCE M. ANDERSON ,Immobilization, and Transglycosidation
DAVID A . YOST, ANDCONSTANCE D . ANDERSON
173
Section VII . Flavins and Derivatives
31 . Luminometric Determination of Flavin Adenine KARL DECKER AN DDinucleotide
ARI HINKKANEN
185
32. Heavy Riboflavin Synthase from Bacillus subtilis ADELBERT BACHER
192
33 . Separation of Flavins and Flavin Analogs by High- ROBERT P . HAUSINGER ,Performance Liquid Chromatography
JOHN F . HONEK, AN DCHRISTOPHER WALSH
199
34 . Preparation, Properties, and Separation by High- PETER NIELSEN ,
Performance Liquid Chromatography of Ribo- PETER RAUSCIIENBACH, AN D
flavin Phosphates
ADELBERT BACHER
20 9
35 . Competitive Binding Assays for Riboflavin and Ri- HAROLD B . WHITE, II]
221boflavin-Binding Protei n
36. Isolation of Avian Riboflavin-Binding Protein
MARK S . MILLER AN D
HAROLD B . WHITE, III
22 7
37 . Fluorometric Titration of Urinary Riboflavin with JERRY ANN TILLOTSON AN D
an Apoflavoprotein
MARK M . BASHOR
23 4
3$. Sensitive Radiosubstrate/High-Performance Liq- SANG-SUN LEE AN D
uid Chromatography Assays for Flavocoen- DONALD B . MCCORMICK
23 7zyme-Forming Enzyme s
39 . 'H NMR Spectral Analysis of the Ribityl Side GARY WILLIAMSON AN D
Chain of Riboflavin and Its Ring-Substituted DALE E . EDMONDSON
24 0Analogs
Section VIII . Pteridines, Analogs, and Pterin Coenzymes (Folate)
40 . Purification of Rat Liver Folate-Binding Protein : ROBERT J . COOK AN DCytosol 1
CONRAD WAGNER
25 1
41 . Dimethylglycine Dehydrogenase and Sarcosine ROBERT J . COOK AN DDehydrogenase : Mitochondrial Folate-Binding CONRAD WAGNER
25 5Proteins from Rat Live r
42 . Membrane-Associated Folate Transport Proteins G . B . HENDERSON AND
F. M . HUENNEKENS
260
43 . High-Performance Liquid Chromatographic Sepa- SUSAN D . WILSON ANDration of the Naturally Occurring Folic Acid De- DONALD W . HORNE
26 9rivative s
44 . Separation of Pteridines from Blood Cells and HANS-JÖRG ZELTLER AN D
Plasma by Reverse-Phase High-Performance BERTA ANDONDONSKAJA-
Liquid Chromatography
RENZ
27 3
45 . Separation of Methotrexate Analogs Containing JAMES H . FREISHEIM AN D
Terminal Lysine or Ornithine and Their Dansyl A . ASHOK KUMAR
29 3Derivatives by High-Performance Liquid Chro-matography
46 . Determination of Pterins by Liquid Chromatogra- CRAIG E . LUNTE AND
phy/Electrochemistry
PETER T . KISSINGER
300
47 . Preparation of (6S)-5-Formyltetrahydrofolate La- RICHARD G . MORAN ,
beled at High Specific Activity with "I C and 3H KHANDAN KEVOMARSI, AN D
PAUL D . COLMAN
309
48. Tissue Folate Polyglutamate Chain Length Deter- D. G . PRIEST AN Dmination by Electrophoresis as Thymidylate M . T. DoIG
31 3Synthase-Fluorodeoxyuridylate Ternary Com -plexe s
49 . Preparative Electrochemical Reduction of Ptero- PETER B . RowE AN Dylpentaglutamate
GARRY P . LEWIS
31 9
50 . Identification of Folylpoly(y-glutamate) Chain BARRY SHANE
32 3Length by Cleavage to and Separation of p -Aminobenzoylpoly(y-glutamates )
51 . Detection of p-Aminobenzoylpoly(y-glutamates) PETER C . LOEWEN
330Using Fluorescamin e
52 . Preparation and Analysis of Pteroylpolyglutamate ROWENA G . MATTHEWS
33 3Substrates and Inhibitors
53 . Analysis of Methotrexate Polyglutamate Deriva- B . A . KAMEN AN Dtives in Vivo
N . WINJCK
339
54 . Radioenzymatic Assay for Dihydrofolate Reduc- M . PERWAIZ IQBAL AN Dtase Using [3 H]Dihydrofolate
SHELDON P . ROTHENBERG
346
55 . Bacterial Folylpoly(y-glutamate) Synthase-Dihy- ANDREW L . BOGNAR AN Ddrofolate Synthase
BARRY SHANE
349
56 . Dihydrofolate Reductase : A Coupled Radiometric PHILIP REYES AN DAssay
PRADIPSINH K . RATHOD
36 0
57 . Purification of Folate Oligoglutamate : Amino Acid Tom BRODY ANDTranspeptidase
E . L . R . STOKSTAD
36 7
58 . Methylenetetrahydrofolate Reductase from Pig ROWENA G . MATTHEWS
37 2Liver
59 . Enzymatic Assay of 5-Methyl-L-tetrahydrofolate PHILIP A . KATTCHEE AN DROBERT W . GUYNN
38 1
60 . Purification of 5,10-Methenyltetrahydrofolate LARS G . LJUNGDAHL ANDCyclohydrolase from Clostridium formico- JOAN E . CLARK
385aceticum
61 . Purification and Properties of 5,10-Methylene- JOAN E . CLARK AN Dtetrahydrofolate Reductase from Clostridium LARS G . LJUNGDAHL
392formicoaceticum
62 . Assay and Detection of the Molybdenum Cofactor R . V . HAGEMAN AN DK . V . RAJAGOPALAN
399
63. Methanopterin and Tetrahydromethanopterin De- JAN T . KELTJENS ,rivatives : Isolation, Synthesis, and Identifica- GERDA C . CAERTELING, ANDLion by High-Performance Liquid Chromatog- GODFRIED D . VOGELS
41 2raphy
AUTHOR INDEX 42 7
SUBJECT INDEX 441
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