Meiosis KM 1 Lesson 1: Sexual reproduction Fusion of two gametes (sex cells) to produce a single...

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Lesson 1: Sexual reproduction

• Fusion of two gametes (sex cells) to produce a single zygote.

• Introduces greater genetic variation, allows genetic recombination.

• With exception of self-fertilizing organisms (e.g. some plants), zygote has gametes from two different parents.

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MEIOSIS

• The process resulting in the creation of sex cells (GAMETES)

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Haploid = 1 set of chromosomes

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Chromosome numbers: Ploidy = number of copies of each chromosome.

All sexually reproducing organisms usually have

even numbers of chromosomes

: diploid (2n) sets of homologous chromosomes!

Haploid = 1 set of chromosome

Diploid = 2 sets of each chromosome

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Homologues

• Chromosomes exist in homologous pairs in diploid cells.

Exception: Sex chromosomes (X, Y).

Other chromosomes are known as autosomes, they have homologues.

Sockosome Example

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Homologues Chromosomes in Humans

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In humans cont’d…

• 23 chromosomes donated by each parent (total = 46 or 23 pairs).

• Gametes (sperm & ova or egg):– Contain 22 autosomes and 1 sex chromosome.– Are haploid (haploid number “n” = 23 in humans).

• Fertilization results in zygote with 2 haploid sets of chromosomes - now diploid.

– Diploid cell; 2n = 46. (n=23 in humans)

• Most cells in the body produced by mitosis.• Only gametes are produced by meiosis.

Are There Advantages of Diploidy in Sexually Reproducing Organisms?

• Back up instructions if a problem arises with the first set

• Greater diversity of genes that may impact the development of the organism (greater diversity = greater chance for survival in changing environmental conditions – EVOLUTION)

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Roadblock activity…

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Sockosome Example – Mitosis Vs. Meiosis

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Line Up and Expect to Be Divided!!!

Mitosis and Meiosis Comparison…

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Sockosome Example – Mitosis Vs. Meiosis

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Animation

MITOSIS VS. MEIOSIS

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Mitosis vs. meiosis

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Meiosis – key differences from mitosis• Meiosis reduces the number of chromosomes by half.• Daughter cells differ from parent, and each other.• Meiosis involves two divisions, Mitosis only one.• Meiosis I involves:

– Synapsis – homologous chromosomes pair up. Chiasmata form (crossing over of non-sister chromatids).

– In Metaphase I, homologous pairs line up at metaphase plate.

– In Anaphase I, sister chromatids do NOT separate.– Overall, separation of homologous pairs of

chromosomes, rather than sister chromatids of individual chromosome.

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MEIOSIS AND VARIATION!

• During normal cell growth, mitosis produces daughter cells identical to parent cell (2n to 2n)

• Meiosis results in genetic variation by shuffling of maternal and paternal chromosomes and crossing over.

No daughter cells formed during meiosis are genetically identical to either mother or father

During sexual reproduction, fusion of the unique haploid gametes produces truly unique offspring.

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Independent assortment

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Independent assortment

Number of combinations: 2n

e.g. 2 chromosomes in haploid2n = 4; n = 22n = 22 = 4 possible combinations

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In humans

e.g. 23 chromosomes in haploid2n = 46; n = 232n = 223 = ~ 8 million possible combinations!

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Random fertilization

At least 8 million combinations from Mom, and another 8 million from Dad …

>64 trillion combinations for a diploid zygote!!!

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Crossing over

Chiasmata – sites of crossing over, occur in synapsis. Exchange of genetic material between non-sister chromatids.

Crossing over produces recombinant chromosomes.

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Harlequin chromosomes

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Add the variability of crossing over to the variable of all of the different

combinations and sexual reproduction equals…

>>>64 trillion combinations for a diploid zygote!!!

SEXUAL REPRODCTION = DIVERSITY

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Meiosis & sexual life cycles

• Life cycle = sequence of stages in organisms reproductive history; conception to reproduction.

• Somatic cells = any cell other than gametes, most of the cells in the body.

• Gametes produced by meiosis.

Generalized animal life cycle

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Sex is costly!

• Large amounts of energy required to find a mate and do the mating: specialized structures and behavior required

• Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.)

• Genetic costs: in sex, we pass on only half of genes to offspring.

• Males are an expensive luxury - in most species they contribute little to rearing offspring.

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But …

• More genetic diversity: more potential for survival of species when environmental conditions change. – Shuffling of genes in meiosis – Crossing-over in meiosis – Fertilization: combines genes from 2 separate

individuals

• DNA back-up and repair. – Asexual organisms don't have back-up copies of genes,

sexual organisms have 2 sets of chromosomes and one can act as a back-up if the other is damaged.

– Sexual mechanisms, especially recombination, are used to repair damaged DNA - the undamaged chromosome acts as a template and eventually both chromosomes end up with the correct gene.

Problems! Oh No!

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Study Questions

• 1. What happens as homologous chromosomes pair up during prophase I of meiosis?

• 2. How does metaphase of mitosis differ from metaphase I of meiosis?

• 3. What is the sole purpose of meiosis?

• 4. What specific activities, involving DNA, occur during interphase prior to both mitosis and meiosis?

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5. Compare mitosis and meiosis on the following points:

a. number of daughter cells produced.b. the amount of DNA in the daughter cells in contrast to the original cell.c. mechanism for introducing genetic variation.

6.  What is a zygote and how is it formed?7. Draw a nucleotide and then draw a 10 nucleotide linear sequence of DNA.

References

• http://www.google.com/search?q=meisosis+powerpoint&ie=utf-8&oe=utf-8&aq=t&client=firefox-a&rlz=1R1GGGL_en___US350

• http://umhs.eduhsd.k12.ca.us/departments/science/freimann/AP%20Bio%20ppt/Meiosis%20AP.pdf

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