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Materials and MethodsWays to Collect Coral Larvae1. Collect Gametes during Spawning Sites -Kapoho -Collected samples observed in lab -HIMB (Coconut Island, Oahu)2. Settlement Tiles -Newly settled larvae Sites -Kapoho -Leleiwi, Hilo Bay3. Plankton tows Site -Leleiwi, Hilo BayFixing processes: Recently spawned coral larvae -preserved in paraformaldehyde solution Plankton tows -Half of sample preserved in glutaraldehyde solution for plankton counts -Remaining half preserved in paraformaldehyde solution for FISH analysis
Discussion and conclusionsCoral Spawning -Times vary depending on location-Corals may not have finished spawning for the year
Collection-A combination of different methods are needed to successfully collect coral larvae
Plankton Tows-No sampling at night so data is skewed-The net was not big enough and the kayak was not moving fast enough to collect all possible plankton types.-Spawning in area was not confirmed and density of larvae may have been low.
In the future-Test the molecular tag on collected samples.-Study coral larvae ecology.-Study genetic migration among coral colonies.
AcknowledgementsNational Science Foundation (NSF) - Research Experience for
Undergraduates (REU)Pacific Internships Programs for Exploring Science (PIPES)
University of Hawaii at Hilo (UHH)Dr. Misaki Takabayashi
Lisa Adams Lauren Pagarigan
Hawaii Institute for Marine Biology (HIMB)Dr. Evelyn Cox
Alu Lik’e Internship Program Kui’ule
University of Queensland Dr. Selina Ward
IntroductionCorals are the building blocks of tropical and subtropical shallow-water reefs, a very important
habitat in the ocean ecosystem. The dispersal of larvae over distances facilitates genetic mixing between populations of coral reef organisms, hence enhancing genetic diversity within
populations. To understand the genetic flow among coral colonies more needs to be known about coral larvae and larval ecology. The larvae are part of the planktonic community and their
dispersal is controlled by the ocean (Oliver et. al. 1991).
To understand Hawaiian coral ecology work was and is currently being conducted on obtaining spawning data for the Big Island, obtaining preliminary data on larval ecology, and developing a
molecular tagging method
Reproductive Ecology of Hawaiian CoralsKathy Seiber
Tyler TakeashitaJune-August 2005
National Science Foundation-Research Experience for UndergraduatesUniversity of Hawaii at Hilo
Dr. Misaki TakabayashiSharon Ziegler-Chong
Newly settled polyp
Planula larvae
Adult colony spawning
Montipora patula
Montipora capitata
Hilo Bay
Kapoho
Results1.Coral Larvae
-Spawning times that were previously known on other islands were accurate for Montipora flabellata during the June full moon and accurate for M. capitata and M. patula during the July full moon on the Island of Hawaii. There was not any reported spawning of Pavona varians, Porites compressa, and P. lobata during the predicted times on Hawaii.
• Settlement Tiles -Wait for 3 months for results
3. Plankton Counts -Three Way ANOVA Results Statistical test showed that the time of day and before and after spawning did not affect the plankton abundance but the abundance of plankton varied based on the type with phytoplankton having the highest abundance.
Leleiwi, Hilo Bay Kapoho Hawaii Institute of Marine BiologyCoconut Island, Oahu
Pavona varians
Porites compressa
Porites lobata
Settlement Tile Trees
Fungia Scutaria
Cnidarian Larvae
CopepodsMollusks
Crustacean Larvae
Phytoplankton
© Gulko, Coral Reef Ecology
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