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How to Use iVitri®- Straw
system?
Manufacture Instruction
Information of iVitri®-Straw
• iVitri® is individual Vitrification Device,
called iVitri.
• iVitri® -Straw is US patent and patent
pending, and registered product (USPTO),
specifically designed for vitrification by
cooperation between physics and math
scientists, and top embryologist according
to liquid-device dynamics (Liquid droplet
radius is changed base on liquid volume,
however, external osmolality is not
changed with vary liquid volume), which
can be effectively used for freezing oocyte,
embryos and reproductive tissues (ovary
and TESE) in human and mammalian IVF.
Production of Package
Product is individual wrapped
and sterilized device (straw)
Product is individual wrapped and color
sterilized device (straw)
Advantage of iVitri® -Straw
• Special design material
• Special art and clear device
• Easy to use
• Easy to label by writing and label on the
handle
• Color code
• Higher survival rate and pregnancy rate
• Water approval after closing cover
• Multiple purpose for oocyte, embryo,
sperm, tissues.
Intended Use
• iVitri® is consisting primarily of two parts:
device body and outside cover. Each one
labeled with iVitri® Logo in handle as
upside. Patient’s name and ID or DOB can
be labeled by typing and writing in this
side.
BBBB SSSS
DOB: 03-12-65
VITRIFICATION PROCEDURE
• Follow up universal vitrification protocol,
and takes the iVitri® by the labeled side
with patient’ name and ID or necessary
information facing up.
Vitrification Procedure-cont. • Place the iVitri® under a microscope
(iVitri Logo should be up) and adjust the
focus on the black mark of the iVitri® (face
up)
• and put the oocytes or embryos (1~3)
near to the black mark with the minimal
volume of vitrification solution (1~2 μl).
The black mark makes easier to put
outside cover.
Vitrification Procedure-cont.
• and put the oocytes or embryos (1~3) near
to the black mark with the minimal volume of
vitrification solution (1.0~1.5 μl). The black
mark makes easier the cover up.
Vitrification Procedure-cont.
Vitrification Procedure-cont.
•Rapidly plunge the iVitri® into fresh
LN2 holding the IVitri®.
Vitrification Procedure-cont.
•Grab the cover with long tweezers (not surgical
forceps) and plunge it into the liquid nitrogen until stops
burbling, be aware don’t take the iVitri® out of the LN2
while covering up, tight and lock gently.
Vitrification Procedure-cont.
•Place the iVitri® into the cane (goblet) with the cover
facing down and store in LN2 tank.
WARMING PROCEDURE
Take out the patient’s canister from the Dewar and
place it into a LN2 container with LN2 covering
completely the iVitri®, and check patient’s information
on the label.
WARMING PROCEDURE-cont.
With long tweezers grabs the iVitri® body and takes it
off from the cane (goblet).
WARMING PROCEDURE-cont.
With long tweezers grab the cover, and pull cover out
without taking iVitri® body out the LN2, leave it always
inside LN2.
WARMING PROCEDURE-cont.
Quickly take off the iVitri® body (Logo face down) from LN2
and face down into pre-warmed thawing solution (drops or
dish) at 37ºC on the microscope stage or laminar hold surface,
and then follow the warming protocol.
WARMING PROCEDURE-cont.
completing recovery: Oocytes for 2 hours and Embryos for 3
hours.
Clinical Trial Data Analysis
Alteration of osmolality may seriously impact on the
survive rates of oocyte and embryos after warming,
consequently decrease pregnancy rate
Liquid volume and droplet radius can directly impact on
the alteration of osmolality, consequently decreased the
survive rate and quality of oocyte and embryos after
warming
Comparison of survive rates using three different vitri-
devices with loading vary volume of vitrification liquid from
0.5 to1.5µl containing bovine oocytes（900 oocytes)（%）
84
86
88
90
92
94
96
98
100
cryoleaf Cryotop iVitri
0.5µl
1.0µl
1.5µl
Comparison of survive rates using three different vitri-
devices with loading vary volume of vitrification liquid
from 0.5 to1.5µl containing oocytes and blastocyst（350
MII oocytes，360 blastocysts，2-3 oocytes or blastocysts
/device)（%）
90
91
92
93
94
95
96
97
98
99
cryoleaf Cryotop iVitri
Oocyte
Blastocyst
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