LUSH PHEROMONE

LUSH PHEROMONE

Manhasset Science ResearchJillian Wong

NEED

http://www.cdc.gov/Features/dsMalariaSurveillance/

NEED

http://www.cdc.gov/Features/dsMalariaSurveillance/dsMalariaSurveillance_450px.gif

Knowledge Base

• Pheromones- a chemical substance secreted by many animal species that alter behavior

5R6S-6-acetoxy-5-hexadecanolide

http://www.pherobase.com/pherobase/gif/5R6S-6-acetoxy-5-hexadecanolide.GIF

Knowledge Base

OBP-odorant binding protein

University of Colorado

http://juliet.med.jhmi.edu/~bianchet/obp1.gif

Literature reviewRockefeller center

Literature Review

• Barbosa, R. MR, Souto, A., Eiras, A.E, Regis, L. 2007. Laboratory and field evaluation of an oviposition trap for Culex quinquefasciatus (Diptera: Culicidae)

Literature Review

• .S. Ha, D.P Smith. 2006. A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate-Induced Responses in

Drosophila

Literature review

Literature Review

Purpose

• The purpose of this experiment is to measure the reaction of the Mutant lush drosophila and the regular drosophila when it comes in contact with 6-Acetoxy-5-hexadecanolide

Hypothesis

• Alternate Hypothesis- whenever the Mutant lush drosophila are in the presence of the pheromone they will not have a response, or they will not have a strong response to the pheromone. However, when the regular drosophila are in the presence of the pheromone, they will have a stronger reaction to it.

• Null Hypothesis-when the mutant Drosophila are within the presence of the drosophila, there will be no significant decrease in their reaction, and when the regular drosophila are within the presence of the pheromone there will be no significant increase in reaction.

Oviposition trap BR-OVTProtocol

http://www.scielo.br/img/revistas/mioc/v102n4/5763fig1.gif

The Effects of 6-acetoxy-5-hexadecanolide on drosophila melanogaster

Lush mutant drosophila n=20

Regular drosophila n=20

Group 2

Group 3

Group 4

Group 5

Group 6

Group 1

Group 8

Group 7

Test in oviposition trap BR-OVT with the measurements of 1, 1.5, 2, and 2.5 µl

Run 80 trials, each for 10 minutes, record number of flies that are attracted to the food during each trial

Compare means, then analyze using SPSS, with an ANOVA and a Scheffe-post-hoc test,

Do-ability

• Most of what is needed is already in the lab. The only problem is finding the pheromone.

BudgetVendor Order # quantity Description Price TotalCarolina 215052 1 Micropipette tips (1-200 ul) yellow 22.50 22.50

Wards 15 V 1925 1 VWR Signature variable volume Pipettors 245.00 245.00

Bibliography• Department of Pharmacology and Center for Basic Neuroscience, University of Texas

Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9111, USA• Ha, Tal Soo, and Dean P. Smith. "A Pheromone Receptor Mediates 11-cis-Vaccenyl Acetate

Induced Responces in Drosophila." The Journal Of Neuroscience (2006): 8727-733.• Jin, Xin, Tal Soo Ha, and Dean P. Smith. "PNAS." SNMP is a signaling component required for

Pheromone Sensitivity in Drosophila 31st ser. 105 (Ausgust 5, 2008): 10996-1001.• "Pheromone." McGraw-Hill Encyclopedia of Science & Technology. 8th ed. 13 vols.

Mechanicsburg: McGraw-Hill, 1997. 397-98.• Smith, Dean P. "Odor and Pheromone detection in Drosophila melanogaster." Pflugers Arch-Eur

J Physiol (2007): 749-58.• University Of Texas Southwestern Medical Center At Dallas. "Researchers Find How Protein

Allows Insects To Detect And Respond To Pheromones." ScienceDaily 27 January 2005. 4 May 2009 <http://www.sciencedaily.com /releases/2005/01/050124005139.htm>.

• UT Southwestern Medical Center. "Unique Pheromone Detection System Uncovered." ScienceDaily 27 June 2008. 29 April 2009 <http://www.sciencedaily.com /releases/2008/06/080626125825.htm>.

• Xu P-X, Atkinson R, Jones DNM, Smith DP (2005) Drosophila OBP LUSH is required for activity of pheromone sensitive neurons. Neuron 45:193-200

• "Wake Up and Smell The Pheromones." The Rockefeller University - Laboratory of Neurogenetics and Behavior. 20 Jan. 2005.