LIGHTrun Brochure GATC

7/16/2019 LIGHTrun Brochure GATC

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 L i g  h t - s p e e d 

 s e q  u e n c i n g  

+ 

 l i g  h t  p r  i c e

WWW.GATC-BIOTECH.COM

w w w . g a t c - b i o t e c h . c o m / l i g h t r u n

 N E W 

s e q u e n c i n g s e r v i c e :

   barcode order on l y

  no  sequenc ing order

   free  samp le  s h ipmen t

 Da ta de l i vered 

 from  8 am – 1 pm 

ne x  t da y!

 Any qu

customers

Germany

Great Britai

France

Sweden

w w w

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Sample requirements

Puried plasmid DNA or puried PCR fragments with primer premixed in tubes

Primer characteristics

• Themeltingtemperature(T M) of the primer should be between 52° and 58°C and

the length should be between 17 – 19bp. Ideally, the GC content of an 17mer should

be 10 G+C; for an 18mer 8 – 9 G+C and for a 19mer 7 – 9 G+C.

• GorCshouldbeatthe3’end,butnotmorethan3GsorCs.

• Theprimersequenceshouldbeagoodmixofall4nucleotideswithnomorethan

4identicalbasesinarow(AAAAorGGGG).

 To ensure the best possible quality of sequence data, the total volume of the sample cannot be less than 10 µl.We recommend measuring the DNA concentration on an agarose gel. Sequencing reactions cannot be repeated andall reactions have to be paid. Your DNA material cannot be s tored. Thank you for your understanding.

 Template DNA of 5 µl with either of following concentrations:•PlasmidDNA(puried):80–100ng/µl•PCRproduct(puried):20–80ng/µl

 Add 5 µl of primer with following concentration:•5µM(5pmol/µl)

Pleasesendtotalsampleamountof10 µl in 1,5 ml tubes.

2.1.

Sampl

1. GATC Co

 Therearem

sample ship

2. FREE shi

Ifthereisn

forshipmen

3. By post 

Ifthereisn

tosend,ple

toensuresa

GATC Biote

European C

Gottfried-Ha

51105 Köln

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Order LIGHT run sequencingbarcodes

Go to www.gatc-biotech.

com/lightrun and click on

“Order LIGHT run barcodes”.

Choose service type and order

your LIGHT run sequencing

barcodes. They will be sent to

you without delay.

LIGHT run in 5 easy steps:

Premix templateand primer 

 Add to your tube:

5 µl template + 5 µl primer

(accordingto

sample requirements).

Label tubes with barcodes  Attach one part of the barcode

to the tube and keep the other

part for your lab record.

 Afx the barco

in the front of the tu

Pleasedon‘tusepara

to seal your tube

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LIGHT run barcode types

LIGHT run tubesdelivery time: 1 day 

upon sample receipt  

LIGHT run NXP tubesdelivery time: 8 am – 1pm

nextday,aftersampledrop-off 

LightRunfor Tubes

Seq. ID12AA34    L

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LightRunfor Tubes

Seq. ID12AA34    L

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LIGHT run 96 well platesdelivery time: 1 day 

upon sample receipt  

LIGHT

Single read

For tubes: eFor 96 well

Nextdaydatad

LIGH

up

delive