Length effects of cylindrical polymer brushes on their in vitro and … · Length effects of...

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Support Information for

Length effects of cylindrical polymer brushes on their in vitro and in vivo

properties

Zhengkui Zhanga, Ling’e Zhanga, Jiaojiao Zhaob, Cheng Lia, Wei Wu*a, Xiqun Jianga

aDepartment of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, People’s Republic of

China

bJiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu People’s

Republic of China

* To whom correspondence should be addressed

Email: wuwei@nju.edu.cn

Electronic Supplementary Material (ESI) for Biomaterials Science.This journal is © The Royal Society of Chemistry 2019

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Table of Contents

1. Supporting Figures S1-S10

2. Captions of Supporting Movie 1, Movie 2

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1. Supporting Figures S1-S10

Retention time (s)

250 300 350 400 450 500 550

Fig. S1. GPC curves of the PGMA for the syntheses of CPBs 1 (black), 2 (red) and 3 (blue).

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Fig. S2. 1H NMR spectra of PGMA in CDCl3 (top) and PGA in (CD3)2CO (bottom) for the

synthesis of CPBs 1.

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3500 3000 2500 2000 1500 1000

20

40

60

80

100

Tra

nsm

itta

nce (

%)

Wavenumber (cm-1)

2100 cm-1

Fig. S3. FT-IR spectrum of PGA for the synthesis of CPBs 1.

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a

b

Fig. S4. 1H NMR spectra of CPBs-Boc 1 (a) and CPBs 1 (b) in D2O.

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Fig. S5. 3D AFM images of CPBs 1 (a), 2 (b) and 3 (c), and cross-sections (red line) including

AFM height images of CPBs 1 (d), 2 (e) and 3 (f) adsorbed on mica from dilute water

solutions. Scale bars = 100 nm.

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012

.5 25 50 100

200

0

20

40

60

80

100

CPBs 1 CPBs 2 CPBs 3

a

Concentration(μg/ml)

Rela

tive c

ell v

iab

ilit

y(%

)

012

.5 25 50 100

200

0

20

40

60

80

100

CPBs 1 CPBs 2 CPBs 3

b

Concentration (μg/ml)

Rela

tive c

ell v

iab

ilit

y (

%)

0

12.5 25 50 10

020

0

0

20

40

60

80

100

CPBs 1 CPBs 2 CPBs 3

b

Concentration (μg/ml)

Rela

tive c

ell v

iab

ilit

y (

%)

Fig. S6. In vitro cytotoxicities of CPBs 1, 2 and 3 against SH-SY5Y (a) and HUVEC (b) cells

determined by MTT assay after 24 h incubation.

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Fig. S7. Permeabilities of CPBs 1 and CPBs 2 in MCs. Typical Z-stack images of SH-SY5Y MCs

coincubated with FITC-labeled CPBs 1 and RBITC-labeled CPBs 2 for 4 h acquired from the

center to the top of the spheroid in 20 μm intervals. Scale bars = 50 μm.

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Fig. S8. Permeabilities of CPBs 1 and CPBs 3 in MCs. Typical Z-stack images of SH-SY5Y MCs

coincubated with FITC-labeled CPBs 1 and RBITC-labeled CPBs 3 for 4 h acquired from the

center to the top of the spheroid in 20 μm intervals. Scale bars = 50 μm.

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0 2 4 6 8 10 120

10

20

30

40

50CPBs 1

CPBs 3

Time (h)

% ID

/mL

pla

sm

a

Fig. S9. Evolution with time of the concentrations of the FITC-labeled CPBs 1 and 3 in the

plasma of H22 tumor-bearing mice after tail-vein injection of the FITC-labeled CPBs 1 and

3, respectively.

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Fig. S10. (a) CLSM images of RAW264.7 cells after 4 h incubation with the FITC-labeled CPBs

1 (left) and 3 (right) at 37 oC, respectively. (b) MFI in RAW264.7 cells measured by flow

cytometry after 4 h incubation with the FITC-labeled CPBs 1 and 3 at 37 oC, respectively.

Data as mean values ± S.D. (n = 3).

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2. Captions of Supporting Movie 1, Movie 2

Supporting Movie 1. 360° rotational views of the 3D whole-body microPET images of a

subcutaneous H22 tumor-bearing mouse at different time points after tail-vein injection of

the 18F-labeled CPBs 1.

Supporting Movie 2. 360 rotational views of the 3D whole-body microPET images of a

subcutaneous H22 tumor-bearing mouse at different time points after tail-vein injection of

the 18F-labeled CPBs 3.