View
213
Download
0
Category
Tags:
Preview:
Citation preview
1
complexity
egg sperm
Development
developmental capacitydevelopmental stage
adult differentiated
Progressive restriction
Lecture 10 – Stem cells and regenerative
medicine
Zygote (fertilized egg)
Embryo
Blastocyst
Developmental potential – mammals
2
Differentiated
Developmental potential – plants
Culture leads to dedifferentiation
callus formation
Embryo formation
Differentiated plant cells are totipotent!genomic equivalence
Plant is genetically identical - cloningPlant growth
Lecture 10 – Stem cells and regenerative
medicine
3
Cloning with somatic, differentiated cells
Lecture 10 – Stem cells and regenerative
medicine
4
Stem Cell: undifferentiated, capable of continuous proliferation
Pluripotent: 3 germ layers (endoderm, mesoderm, ectoderm)
Totipotent: all cell types (embryonic & extraembryonic)
Developmental potential – mammals
Asymmetric division
Stem Cell Niche
Lecture 10 – Stem cells and regenerative
medicine
ES cells from ICM
5
Developmental potential – mesenschymal stem cells (MSC) in mammals
Specification of Developmental fates
Distinct gene expression
programs!
Lecture 10 – Stem cells and regenerative
medicine
6
Pluripotent
developmental capacity
Differentiated
Genomic equivalence
multipotent
bonemuscle
nerve
embryonicstem cells
Tissue Rejection
Adult stem cells: skin, intestine, blood… Bone:
a. hematopoieticb. multipotent stromal cells
bone/muscle
Lecture 10 – Stem cells and regenerative
medicine
Therapeutic cloning
Cell/tissue immune compatibility
7
Gene Therapy – direct vs. cell based
Lecture 10 – Stem cells and regenerative
medicine
8
Pluripotent stem cells
Potential usesRepair mutation, reimplant patient cells
OCT4, SOX2, KLF4, MYC
- induce pluripotency • Oct4, SOX2, KLF4, cMyc• genetically corrected iPS cells
- reintroduce cells
- induce cell type desired
- use fibroblast, repair defect
Lecture 10 – Stem cells and regenerative
medicine
9
Pluripotent stem cells 2008 - Eggan labadult fibroblasts - patient matched!OCT4, SOX2, KLF4, MYC
Lecture 10 – Stem cells and regenerative
medicine
Recommended