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iGEM Meeting. 3-19-2010. Brochure and Sponsorship Package. Lab Update Regional Conference Contacts for further publicity. Dave Leake from Surrounded by Science . Credit Hours Safety information? No meeting Next week – Happy Spring Break! Movie Night April 1 st. Francis’ Lab Work. - PowerPoint PPT Presentation
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3-19-2010
Brochure and Sponsorship Package.Lab UpdateRegional ConferenceContacts for further publicity. Dave Leake
from Surrounded by Science.Credit HoursSafety information?No meeting Next week – Happy Spring
Break!Movie Night April 1st
sRNA resubmissionMicC Mutagenesis
StandardizationAll target sequences contain an NheI siteAll target sequences contain one of the
following (EcoRI, SpeI, PstI, XbaI)Site directed mutagenesis
Each isocaudamer site must be removed before submission
E. Coli micC - ompCS. Typhimirium micC – ompC, ompD
6 MicC mutants5’ END3’ TerminatorRBS+67 siteRandom
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