IgE Test Serology

Immunoglobulin E Disease

Group 23H-Medical Technology

#8 Carla, Glenn Christian#9 Cervantes, Andrea Lourdes

#10 Cheung, Terence#11 Cruz, Carl Dustin

#12 Dantes, Ralph Laurence#13 De Asis, Ana Mikaela

Clinical Manifestation

caused by the release of preformed and newly

synthesized mediators from mast cells and basophils.

Symptoms depends on route of administration.

Anaphylaxis

Most severe type of allergic reaction

Acute reaction with simultaneous involvement of multiple organs

Triggered by glycoproteins or large polypeptide

Agents: Induce Anaphylaxis

• Bees• Wasp • Hornets

Venom

• Peanuts • Dairy products• Shellfish

Foods

• PenicillinDrugs

Signs

Laryngeal edema

Bronchospasm

Skin manifestation (hives)

Diarrhea

Intractable shock

Rhinitis

Most common form of allergy

Symptoms include • runny nose • nasal congestion• itching of the eyes and nose

Airborne• Pollen• mold spores• animal dander• dust

Complication

Sinusitis

Eustachian tube dysfunction

Otitis media

Food allergies

limited to gastrointestinal tract

Diarrhea

Vomiting

Cramping• Cause hives on the skin • Rhinitis • Eczema

Procedure

Open the foil pouch by tearing at the notch and remove the test device and dropper.

Holding the dropper vertically, add four drops of serum or directly to the sample well "S" of the test device.

Read the result at five minutes.

Procedure

Wait for 5 minutes.

Principle of IgE testing

-utilizes the "sandwich"

immunodetection

principle dye-

conjugated polyclonal antibody against

human IgE

-Labeled antibody-dye

conjugate binds to the human IgE molecules present in

the specimen forming an

antigen-antibody-dye

complex

-purple band in control zone "C"

indicates the test is

functioning properly

-purple band in the test zone "T"

indicates IgE present

along with positive

control band

Results and Interpretation

Negative Result

Results and Interpretation

Positive Result

Results and Interpretation

Invalid Result

Thank You!