Hormones in the Environment - uni-stuttgart.de · A B C 1 2 denitrification tank aeration tank...

A

B

C

1

2

denitrification tank aeration tank

rotating biologicaldiskcontactors

secondaryclarifiers(1– 3)

3

oxidationditch

secondaryclarifier(oxidationditch)

microsieves

effluent

grids andsieves

aerated sludge process (A)rotating biological disk contactors (B)oxidation ditch (C)

influent

aeratedgritchamber primaryclarifier

Biological Wastewater Treatment

A

B

C

1

2

denitrification tank aeration tank

rotating biologicaldiskcontactors

secondaryclarifiers(1– 3)

3

oxidationditch

secondaryclarifier(oxidationditch)

microsieves

effluent

grids andsieves

Arotating biological disk contactors

oxidation ditch

influent

aeratedgritchamber primaryclarifier

BC

aerated sludge process

Mechanical Wastewater Treatment

Results (mechanical wastewater treatment): EEQvaluesoftheinfluentandtheeffluentoftheprimaryclarifiertaking intoaccounttheaveragehydraulicretentiontime(Nov8,2006)

Hormones in the Environment - Quantitation of the Total Estrogenic Activity

of Wastewater Samples with a Bioassay

www.iswa.uni-stuttgart.de

Contact

: Prof.Dr.rer.nat.JörgW.Metzger,ISWA,Bandtäle2,70569Stuttgart

joerg.metzger@iswa.uni-stuttgart.de,www.iswa.uni-stuttgart.de/ch

CorneliaKlaus,BiljanaMarić,AndreaKern,BertramKuchandJörgW.Metzger InstituteforSanitaryEngineering,WasteWaterQualityandSolidWasteManagement,Dept.ofHydrochemistry

Universität Stuttgart

Aim of this Study: • Input/output balance of the total estrogenic activity in the sewage treatment plant (STP) of Stuttgart-Büsnau in Germany • Determination of the efficiencies of purification of the three different biological treatment steps

Material and Methods:Sampling: grabsamplesofeachsewage treatmentsteptakingintoaccount thehydraulicretentiontimeifpossibleinaddition:24hourscomposite samplesofinfluentandeffluent duringoneweekSampleTreatment: centrifugation filtration solidphaseextraction(RP-18)DeterminationofEstrogenicity: biologicalsummaryparameter expressedasanestradiolequivalent concentration(EEQ)inng/LE-Screenassay:invitrocell proliferationassaybasedonthe humanestrogenreceptor-positive breastcancercell-lineMCF-7 generalestimationoftheuncertainty ofmeasurementaspartofthe validationprocessforthesuitabilityof thisbioassay

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Results (24 h composite samples): Diminutionofestrogenicactivityduringwastewatertreatment -EEQvaluesof24hcompositesamples(Nov21-28,2006)

Conclusions:

• TheE-Screenassayisverysuitablefor balancingestrogenicactivityofwastewater samples(datavariationmax.oneorderof magnitude)• EEQ values of the influent rangedbetween 12.6ng/Land72.7ng/L(median23.8ng/L); those of the effluentwerefoundtobe permanentlybelow2.5ng/L(median1.6ng/L)

• Theoveralleliminationefficiencyofthewholeplantisconstantand stable:mechanicalwastewatertreatmentdidnotleadtoany significantelimination,whereasbiologicaltreatmentreducedestrogenicity toagreatextentwithslightdifferencesbetweenthethreetechnologies• Thereisasmallbutconstantinputofestrogenactivecompounds intothereceivingwater:informationonthedilutionfactorandthekindof differentestrogeniccompoundsisrequiredtoevaluateanecotoxicological riskpotential

Results (biological wastewater treatment): Eliminationofestrogenicactivityinwastewater Eliminationofestrogenicactivityinwastewater -activated sludge process -rotating biological disk contactor

Eliminationofestrogenicactivityinwastewater -oxidation ditch

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EEQ(ng/L)

influent primaryclarif ier

denitrif icationtank

aerationtank secondaryclarif ier1

secondaryclarif ier2

secondaryclarif ier3

eff luent

Oct11,2006 Oct18,2006 Jan24,2007

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influent primaryclarif ier

roll1 roll2 roll3 secondaryclarif ier1

effluent

EEQ(ng/l)

Oct18,2006 Jan10,2007 Jun4,2007

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influent oxidationditch secondaryclarif ier(OD)

effluent

Oct12/13,2006 Oct17/18,2006 Jan16/17,2007

Reference: Klaus,Cornelia(2007):ErmittlungdesVorkommensundderElimination vonestrogenaktivenSubstanzenimLehr-undForschungsklärwerkStuttgart-Büsnau -BewertungdesE-ScreenAssaysalsinvitro-Testverfahren,diplomathesis,UniversitätStuttgart

→ nosignificant decreasein estrogenicity

→ average efficiencyof removalduring thatweek: 92.2%

Eliminationefficiencies(%)ofbiologicalwastewatertreatment (effluentofthebiologicaltank/rawinfluent)

1.sampling 2.sampling 3.sampling average

activatedsludgeprocess 92.3 97.8 94.4 94.8

rotatingbiologicaldiskcontactors 66.7 82.3 76.3 75.5

oxidationditch 91.0 90.5 93.7 91.7

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EEQ(ng/L)

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influent effluent

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EEQ(ng/L)

7.30a.m./8.55a.m. 7.45a.m./9.10a.m. 8.00a.m./9.25a.m. 8.15a.m./9.40a.m.

inf luent eff luentprimaryclarif ier

samplingsites(grabsamples)