High Sensitivity RNA ScreenTape® EUKARYOTIC RNA … · F6 7.5 2.1 99800 28 RNA concentration...

Filename: emilM - HSRNA - 2017-06-07 - 01.HSRNA

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High Sensitivity RNA ScreenTape® EUKARYOTIC RNA ANALYSIS Page 1 of 64

TapeStation Analysis Software A.02.01 © Agilent Technologies, Inc. 2015 Generated: 07-Jun-2017

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Sample Info

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsEL1 - - 3750 Electronic Ladder Ladder

A1 2.1 - 190 HS-1001RNA concentrationoutside recommendedrange for RINe

B1 2.1 - 223 HS-1002RNA concentrationoutside recommendedrange for RINe

C1 2.2 - 180 HS-1003RNA concentrationoutside recommendedrange for RINe

D1 2.0 - 118 HS-1004RNA concentrationoutside recommendedrange for RINe

E1 2.1 - 172 HS-1005RNA concentrationoutside recommendedrange for RINe

F1 2.1 - 193 HS-1006RNA concentrationoutside recommendedrange for RINe

G1 2.2 - 209 HS-1007RNA concentrationoutside recommendedrange for RINe

H1 2.2 - 164 HS-1008RNA concentrationoutside recommendedrange for RINe

A2 2.2 - 226 107RNA concentrationoutside recommendedrange for RINe

B2 3.0 - 414 117RNA concentrationoutside recommendedrange for RINe

C2 1.2 - 273 119RNA concentrationoutside recommendedrange for RINe

D2 2.1 - 245 121RNA concentrationoutside recommendedrange for RINe

E2 2.3 - 227 123RNA concentrationoutside recommendedrange for RINe

F2 2.0 - 263 124RNA concentrationoutside recommendedrange for RINe

G3 1.9 - 254 91RNA concentrationoutside recommendedrange for RINe

H3 2.8 - 283 106RNA concentrationoutside recommendedrange for RINe

G4 8.1 2.1 8290 67H4 8.8 4.3 1580 68A5 8.6 3.0 1510 38B5 4.4 0.6 1770 39C5 7.9 2.0 8790 42D5 2.3 - 2820 45E5 7.4 2.2 3160 46F5 4.4 0.8 7260 47G5 4.9 1.0 3160 48H5 6.3 2.1 5300 50A6 7.9 1.9 7350 10B6 8.3 2.3 4510 11C6 7.9 2.2 14500 15D6 8.1 2.6 13600 22E6 8.5 2.5 23300 26

F6 7.5 2.1 99800 28RNA concentrationoutside recommendedrange for RINe

G6 6.8 1.6 168000 32RNA concentrationoutside recommendedrange for RINe

H6 4.6 0.5 119000 36RNA concentrationoutside recommendedrange for RINe

A7 8.7 3.1 3870 1B7 8.9 2.6 9200 2

C7 6.9 1.6 78100 5RNA concentrationoutside recommendedrange for RINe

D7 8.7 3.1 1470 7E7 7.7 1.7 24600 73F7 8.0 1.9 24600 77

A8 8.0 2.0 21100 140

B8 7.3 1.8 81300 143RNA concentrationoutside recommendedrange for RINe

EL1: Electronic Ladder

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsEL1 - - 3750 Electronic Ladder Ladder

Peak Table

Size [nt] Calibrated Conc.[pg/µl]

Assigned Conc.[pg/µl]

Peak Molarity[pmol/l] % Integrated Area Peak Comment Observations

25 700 700 82400 - Lower Marker200 189 - 2770 5.98500 638 - 3760 20.251000 557 - 1640 17.662000 580 - 854 18.424000 634 - 466 20.116000 554 - 272 17.58

A1: HS-1001

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert Observations

A1 2.1 - 190 HS-1001RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker

B1: HS-1002

Sample Table

B1 2.1 - 223 HS-1002RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker

C1: HS-1003

Sample Table

C1 2.2 - 180 HS-1003RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker

D1: HS-1004

Sample Table

D1 2.0 - 118 HS-1004RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker

E1: HS-1005

Sample Table

E1 2.1 - 172 HS-1005RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker

F1: HS-1006

Sample Table

F1 2.1 - 193 HS-1006RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker

G1: HS-1007

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

H1: HS-1008

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

A2: 107

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

B2: 117

Sample Table

Peak Table

25 700 700 82400 - Lower Marker882 41.9 - 140 100.00 18S

C2: 119

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

D2: 121

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

E2: 123

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

F2: 124

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

G2: HS-1009

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1230 7.15 - 17.1 100.00 18S

H2: HS-1010

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

A3: 69

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

B3: 70

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

C3: 71

Sample Table

Peak Table

25 700 700 82400 - Lower Marker661 16.6 - 73.8 100.00 18S

D3: 74

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

E3: 86

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

F3: 88

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1160 7.36 - 18.7 100.00 18S

G3: 91

Sample Table

G3 1.9 - 254 91RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker

H3: 106

Sample Table

H3 2.8 - 283 106RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker804 22.3 - 81.6 100.00 18S

A4: 52

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

B4: 53

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1252 8.59 - 20.2 100.00 18S

C4: 59

Sample Table

Peak Table

25 700 700 82400 - Lower Marker863 7.02 - 23.9 100.00 18S

D4: 60

Sample Table

Peak Table

25 700 700 82400 - Lower Marker887 24.4 - 81.0 100.00 18S

E4: 62

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1384 4.80 - 10.2 100.00 18S

F4: 63

Sample Table

Peak Table

25 700 700 82400 - Lower Marker

G4: 67

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsG4 8.1 2.1 8290 67

Peak Table

25 700 700 82400 - Lower Marker1247 1630 - 3840 32.63 18S3248 3360 - 3040 67.37 28S

H4: 68

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsH4 8.8 4.3 1580 68

Peak Table

25 700 700 82400 - Lower Marker1252 234 - 549 18.86 18S3769 1010 - 785 81.14 28S

A5: 38

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsA5 8.6 3.0 1510 38

Peak Table

25 700 700 82400 - Lower Marker1237 222 - 528 25.24 18S3878 657 - 498 74.76 28S

B5: 39

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsB5 4.4 0.6 1770 39

Peak Table

25 700 700 82400 - Lower Marker1153 257 - 655 62.86 18S3543 152 - 126 37.14 28S

C5: 42

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsC5 7.9 2.0 8790 42

Peak Table

25 700 700 82400 - Lower Marker1131 1680 - 4380 33.05 18S2957 3410 - 3390 66.95 28S

D5: 45

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsD5 2.3 - 2820 45

Peak Table

25 700 700 82400 - Lower Marker791 181 - 673 100.00 18S

E5: 46

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsE5 7.4 2.2 3160 46

Peak Table

25 700 700 82400 - Lower Marker1206 497 - 1210 30.85 18S3384 1120 - 969 69.15 28S

F5: 47

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsF5 4.4 0.8 7260 47

Peak Table

25 700 700 82400 - Lower Marker881 1560 - 5220 55.96 18S2295 1230 - 1580 44.04 28S

G5: 48

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsG5 4.9 1.0 3160 48

Peak Table

25 700 700 82400 - Lower Marker1204 463 - 1130 50.05 18S3416 462 - 398 49.95 28S

H5: 50

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsH5 6.3 2.1 5300 50

Peak Table

25 700 700 82400 - Lower Marker1214 644 - 1560 32.04 18S3262 1370 - 1230 67.96 28S

A6: 10

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1186 1520 - 3770 34.77 18S3047 2850 - 2750 65.23 28S

B6: 11

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1191 802 - 1980 30.48 18S3206 1830 - 1680 69.52 28S

C6: 15

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsC6 7.9 2.2 14500 15

Peak Table

25 700 700 82400 - Lower Marker1174 2720 - 6830 31.56 18S2904 5910 - 5980 68.44 28S

D6: 22

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsD6 8.1 2.6 13600 22

Peak Table

25 700 700 82400 - Lower Marker1144 2290 - 5880 27.84 18S2861 5920 - 6090 72.16 28S

E6: 26

Sample Table

Peak Table

25 700 700 82400 - Lower Marker880 4170 - 14000 28.50 18S2137 10500 - 14400 71.50 28S

F6: 28

Sample Table

F6 7.5 2.1 99800 28RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker790 20300 - 75400 32.14 18S1807 42800 - 69600 67.86 28S

G6: 32

Sample Table

Peak Table

25 700 700 82400 - Lower Marker857 38500 - 132000 37.74 18S1982 63500 - 94200 62.26 28S

H6: 36

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1657 25100 - 44600 64.86 18S1886 13600 - 21200 35.14 28S

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1183 554 - 1380 24.63 18S3219 1700 - 1550 75.37 28S

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1170 1670 - 4190 27.54 18S2976 4380 - 4330 72.46 28S

Sample Table

Peak Table

25 700 700 82400 - Lower Marker822 18400 - 66000 38.02 18S1936 30000 - 45700 61.98 28S

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsD7 8.7 3.1 1470 7

Peak Table

25 700 700 82400 - Lower Marker1150 213 - 544 24.36 18S3563 660 - 545 75.64 28S

E7: 73

Sample Table

Peak Table

25 700 700 82400 - Lower Marker771 5710 - 21800 36.80 18S1851 9800 - 15600 63.20 28S

F7: 77

Sample Table

Well RINe 28S/18S (Area) Conc. [pg/µl] Sample Description Alert ObservationsF7 8.0 1.9 24600 77

Peak Table

25 700 700 82400 - Lower Marker769 5590 - 21400 34.59 18S1855 10600 - 16800 65.41 28S

G7: 113

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1822 40600 - 65600 62.28 18S2057 24600 - 35200 37.72 28S

H7: 138

Sample Table

Peak Table

25 700 700 82400 - Lower Marker814 14400 - 51900 32.09 18S1888 30400 - 47400 67.91 28S

A8: 140

Sample Table

Peak Table

25 700 700 82400 - Lower Marker1142 4440 - 11400 33.09 18S2812 8980 - 9390 66.91 28S

B8: 143

Sample Table

B8 7.3 1.8 81300 143RNA concentrationoutside recommendedrange for RINe

Peak Table

25 700 700 82400 - Lower Marker760 18200 - 70300 36.08 18S1772 32200 - 53400 63.92 28S

C8: 147

Sample Table

Peak Table

25 700 700 82400 - Lower Marker807 16200 - 59000 35.55 18S1852 29300 - 46600 64.45 28S

High Sensitivity RNA ScreenTape® EUKARYOTIC RNA … · F6 7.5 2.1 99800 28 RNA concentration...

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