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Grading of smears examined in ordinary microscopy at 1000X magnification after ZN staining. ----- smear contains no AFB in 100 fields. NEG. How will you grade a smear with 9 AFB in 100 fields ?. positive(Actual) 09 AFB. ------ Smear with more than 10 AFB per field. 3 +. - PowerPoint PPT Presentation
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Grading of smears examined in ordinary microscopy at 1000X
magnificationafter ZN staining
----- smear contains no AFB in 100 fields.
NEG
How will you grade a smear with 9 AFB in 100
fields ?
------ Smear with more than 10 AFB per field.
3 +
A smear with 1 to 9 bacilli in majority of the fields will be graded as:
2 +
Smear grade: 2 +(1-9 AFB /field in at
least 50 fields)
Smear Grade: 1 +
10 - 99 AFB in 100 fields
Smear Grade:actual
( < 10 AFB in 100 fields)
Grade the
following smears:
Positive (6AFB)
Positive 1+
1 + (86 AFB/96 HPF)
Positive 2 +
Positive (8 AFB)
Positive 1 +
CreditRevised National Tuberculosis
Control Program-Tuberculosis Research Center
Chennai, India
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