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Flow cytometry From basic theory to practical matters
Flow cytometry From basic theory to practical matters
Martial JAUME(breizh@hku.hk)
HKU-Pasteur Research Centre
Flow cytometry workshopHKU, 21-22 October 2010
A meaningful nameA meaningful name
http://www.bdbiosciences.com/immunocytometry_systems/support/training/online/ITF/index.html
DefinitionsDefinitions
Flow cytometry is a powerful technology for characterizing and analyzing cells. It simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, as they move in a fluid stream through a beam of light
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Flow cytometerFlow cytometer
Background informationabout the instrument
Flow cytometer: 3 subsystemsFlow cytometer: 3 subsystems
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System part 1: FluidicsSystem part 1: Fluidics
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FluidicsFluidics
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Influence of the sheath flow rateInfluence of the sheath flow rate
System part 2: Optics systemSystem part 2: Optics system
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Overview of the optics systemOverview of the optics system
Overview of the optics systemOverview of the optics system
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Light collection through filtersLight collection through filters
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System part 3: ElectronicsSystem part 3: Electronics
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ElectronicsElectronics
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ElectronicsElectronics
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ElectronicsElectronics
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ElectronicsElectronics
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ElectronicsElectronics
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Starting experiment and analyzing dataStarting experiment and analyzing data
Practical considerations and examples of data acquired by flow cytometry
Optical measurement: Light scatteringOptical measurement: Light scattering
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Optical measurement: Light scatteringOptical measurement: Light scattering
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Optical measurement: Light scatteringOptical measurement: Light scattering
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Optical measurement: FluorescenceOptical measurement: Fluorescence
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Optical measurement: FluorescenceOptical measurement: Fluorescence
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Optical measurement: FluorescenceOptical measurement: Fluorescence
Excitation and Emission SpectraExcitation and Emission Spectra
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For further details visit http://www.bdbiosciences.com/spectra/
Multiple colors and spilloverMultiple colors and spillover
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Spillover and signal compensationSpillover and signal compensation
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Spillover and signal compensationSpillover and signal compensation
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Spillover and signal compensationSpillover and signal compensation
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Spillover and signal compensationSpillover and signal compensation
Data collection and displayData collection and display
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Population gatingPopulation gating
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Population gatingPopulation gating
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Population gatingPopulation gating
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Color gatingColor gating
Chart flow of data collection/analysisChart flow of data collection/analysis
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Analyzing histogram plotAnalyzing histogram plot
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Analyzing histogram plotAnalyzing histogram plot
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Analyzing dot plotAnalyzing dot plot
Applications of flow cytometryApplications of flow cytometry
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ImmunityImmunity
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CancerCancer
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Molecular biologyMolecular biology
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Want more…??Want more…??
Interpreting flow cytometry data: a guide for the perplexed.Herzenberg, L. A. et al.Nat Immunol 7, 681-685. (2006)..
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