Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells COUP-TFI COUP-TFII PPAR VDR...

Expression of Nuclear Receptors during Adipogenesis of 3T3-L1 Cells

• COUP-TFI• COUP-TFII• PPAR• VDR• GCNF• ROR• HNF4

• LXR• LXR• RXR• Nur77• REV-ERB• REV-ERB

VDR and COUP-TFII Expression during Adipogenesis of 3T3-L1 Cells

0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D

VDR

COUP-TFII

0 6 12 18 24h 2d 3 4 5 6 7 8d

Induction Medium Differentiation Medium

mRNA

Fu et al., Mol. Endo. 19: 2437 (2005)

COUP-TFIIVDR

LXR α

0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D

0 6 12 18 24h 2d 3 4 5 6 7 8d

Induction Medium Differentiation Medium

NR Expression during Adipogenesis of 3T3-L1 Cells (2)

Fu et al., Mol. Endo. 19: 2437 (2005)

Nur77

PPAR γ

NUR77LXR PPAR

0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D

Actin

COUP-TFI

0 6 12 18 24h 2d 3 4 5 6 7 8d

Induction Medium Differentiation Medium

NR Expression during Adipogenesis of 3T3-L1 Cells

GCNF

Fu et al., Mol. Endo. 19: 2437 (2005)

RXR α

Other Nuclear Receptors

0h 4h 8h 18h 1D 2D 3D 4D 5D 6D 7D 8D

0 2 4 8 18h 1 2 3 4 5 6 7 83T3-L1 cells

Inducing Medium Djffer.Medium

REV-ERB α

REV-ERB β

ROR α

HNF4α

LXR β