Exocytosis and Endocytosis - CellBiology · Single molecule uncoating assay clathrin hsc70 overlay...

Exocytosis and Endocytosis

till.boecking@unsw.edu.au

5 um

http://dx.doi.org/10.1016/S0092-8674(03)01079-1

Exocytosis

Transcription

Endocytosis

Phagocytosis: Neutrophil

Caveolae-lipid recycling -signalling -endocytosis

Peroxisomes- fatty acid metabolism - oxidative reactions (H2O2), e.g. fatty acids, cholesterol, bile salts

Clathrin mediated endocytosis

Massol et al PNAS 2006

Live cell imaging

Heuser 1989

Xing; Böcking; Wolf; Grigorieff; Kirchhausen; Harrison, EMBO J 2010Rapoport, Boll, Yu, Böcking, Kirchhausen, Mol Biol Cell 2008

Single particle uncoating assay

Time

Intensity

2. coupled release

Time

Intensity

1. uncoupled release

Single molecule uncoating assay

clathrin hsc70 overlay

Nature Structural and Molecular Biology, 2011, 18(3), 295-301

Janet Iwasa and Tom Kirchhausen,Cell Biology, Harvard Medical School

Cellular Mechanisms of Health and Disease

SOMS3232

Research internships and Honours projects

Molecular Machines Group