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From: Paul LeeperTo: Maloney, KellySubject: Fwd: Union River bug dataDate: Thursday, April 16, 2020 2:47:03 PMAttachments: pre-EDD Stream_MI_Field Union R 2019.xls
Pre-EDD_MI_Taxonomist Union R 2019.xlsx
CAUTION: This email originated from outside of the organization. Do not click on links or open attachments unlessyou recognize content is safe. Please report suspicious emails here ATTENTION: Ce courriel provient d'une source externe, ne cliquez pas sur les liens et n'ouvrez pas les pièces jointes,à moins que vous en reconnaissiez la source. Veuillez nous aviser ici de tout courriel suspect.
-------- Forwarded Message --------Subject:Union River bug data
Date:Thu, 19 Dec 2019 13:49:10 -0500From:Paul Leeper
To:Meidel, Susanne K CC:Dunlap,Frank
Susanne,
Here are the files for one site downstream of the Graham Lake Dam.If you have any questions or I screwed something up please contact me.
Thanks as always,
Paul
-- Paul C. LeeperMoody Mountain Env.137 Diamond Str.Searsmont, ME 04973
EXHIBIT 13
mailto:moodymtn@tidewater.netmailto:Kelly.Maloney@brookfieldrenewable.commailto:phishing@brookfieldrenewable.commailto:hameconnage@energiebrookfield.commailto:moodymtn@tidewater.netmailto:Susanne.K.Meidel@maine.govmailto:Frank.Dunlap@brookfieldrenewable.comRead Me
Guidance for Completing Biomonitoring Stream Macroinvertebrate Field Data Entry FormLocationPotential Stressor
OptionsNotesAgricultural Runoff
Updated 2/18/2016Above Road CrossingAltered Habitat
General Notes:Below Road CrossingAltered Hydrology
When beginning to enter field data from a new field season, request a new version of this file from the data manager. Prior to entering all field data, load one completed test file to make sure everything works correctly.Above TownAltered Hydrology (Beaver Activity)
A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.Below TownBelow Ski Area
B) Please complete peach-colored fields and do not alter white fields.Above Fish HatcheryBelow Wetland
C) Please do not enter text into empty fields; this will interfere with data processing.Below Fish HatcheryBOD (Low DO)
D) We encourage you to fax us (207/287-7826) original field sheets so we can carry out additional QC.Above WetlandChlorine
E) Please do not change the name of the worksheet 'Stream_MI' as this will interfere with data processing. If you are submitting data for multiple sites, please do so using separate files.Below WetlandGravel Pit
Above POTWImpounded
Note that for all items below only the points that need explanation are annotated.Below POTWInorganic Solids
Above LandfillLake Outlet
1) Site/Sample event info block (first block)Below LandfillLogging
a) Waterbody Name and Town: add the relevant information; exact form or accuracy are not required - these fields are not used for loading data.Below AirportLow DO
b) Station Number: add the 1-3 digit biomonitoring station number without the 'S-' (e.g., 5 or 826); if number is unknown, leave field blank.Below In-Place ContaminationLow Gradient
c) Sampled By and Sample Method: select from drop-down list.Above In-Place ContaminationLow pH
d) Note that Deployment and Retrieval Time come from the Habitat Characteristics section on the paper field sheet.Above Point SourceMetals
e) Deployed Depth Rep 1/2/3: enter the stream depths at the locations where samplers 1-3 were deployed. If an average depth was noted on the field sheet, enter the same value three times. Note that this information also goes in field B48.Below Point SourceNPS Pollution
f) The following info does not get captured in this form:- River Basin, Stream Order - DEP staff will determine these- Directions, Lat/Long coordinates - for new site, enter info in the New Site Information worksheet - Any field sheet comment about 'number of jars' - omitf) The following info does not get captured in this form:- River Basin, Stream Order - DEP staff will determine these- Directions, Lat/Long coordinates - for new site, enter info in the New Site Information worksheet - Any field sheet comment about 'number of jars' - omitAbove Urban NPSNutrients
Below Urban NPSOrganic Solids
2) Physical Characteristics blockAbove Agriculture NPSPesticides
a) Land Use and Location: select as many options from the drop-down list as desired. Do not enter the same option multiple times.Below Agriculture NPSRegulated Flows
b) Location: see columns C and D on this worksheet for a list of options and explanations for some of them.Above Forestry NPSSedimentation
c) Use the Comments fields in Row 62 to make additional notes on Land Use or Location if necessary.Below Forestry NPSSuperfund Site
Above DamOnly used for man-made dams; note info on beaver dam in Comments field, if desired.Thermal
3) Potential Stressor(s) blockBelow DamTidal/Estuary
a) Enter as many options from the drop-down list as desired. Do not enter the same option multiple times.ImpoundmentOnly used for man-made impoundments.Toxic Organics
a) See column F on this worksheet for a list of options.Lake OutletUrban Runoff
c) Use the Comments fields in Row 62 to make additional notes on Potential Stressor(s) if necessary.Main StemOnly used for larger systems.Wetland Headwaters
Above Confluence
4) Physical Characteristics of Bottom blockBelow Confluence
a) Enter percentage values only for the substrate types found; do not enter '0' for types not found.Below Falls
b) Make sure the summed percentage is 100%.Pristine Landscape
Designated Ecoreserve
5) Habitat Characteristics blockNational Park
a) Wetted and Bankfull Width, and Depth: enter a single value, not a range. If a range (or multiple values) was noted on the field sheet, enter the average here and if desired note what the range was in the Comments field in row 62.Minimally Disturbed
b) Make sure the values entered for DO, DO Percent Saturation, Temperature, and SPC match the units supplied. If necessary, convert values e.g. from degrees Fahrenheit to degrees Celsius.
c) Meter # information: the existing set-up assumes that DO and temperature are measured with one meter and SPC/pH with another. If your set-up differs, please make sure to note the meter ID in fields B/F 54 and 56.
d) HETL Folder #: this information is not stored in the database but only entered here as an electronic record.
e) If velocity on the field sheet says "visible" leave velocity value blank. Add a comment in the comment section(s) below: "Visible flow at deployment." or "Visible flow at retrieval." If both deployment and retrieval had only visible flow, put a comment in each comment field.
6) Comments at Deployment and Comments at Retrieval
a) Enter here any relevant comments (i.e. those that could help with data interpretation) that are noted on the field form, including in the Observations block. End comment with a period.
i) If a comment is of a general nature (i.e. is not date-related, e.g. additional information on Location), it can be put in either field A62 or E62.
ii) If a comment relates only to one date, enter it in the relevant Comment field. If the wording of the comment does not include a reference to either deployment or retrieval date (e.g. 'High water on deployment.'), add the date in the comment (e.g. 7/8/11: high water).
&CPage &P of &N
Stream_MI
EDD for Rivers/Streams Macroinvertebrate Field DataLUP values for pull-downs
Waterbody NameUnion RSampled By
TownEllsworthBiomonitoring Unit
Station NumberNumber only (omit ‘S-‘)Acadia National Park
Log NumberHoulton Band of Maliseet Indians
Sampled ByMoody Mountain EnvironmentalListLotic, Inc.
Sample MethodRBG-Rock BagListMoody Mountain Environmental
Deployment Date8/9/19Retrieval Date9/6/19Passamaquoddy Tribe
Deployment Time10:00 AMRetrieval Time12:30 PMPenobscot Indian Nation
Number Deployed3Number Retrieved3Portland Water District
Deployed Depth Rep 179cm-required
Deployed Depth Rep 279cm-required
Deployed Depth Rep 379cm-requiredSample method
RBG-Rock Bag
Physical CharacteristicsRB-Rock Basket
Land Use 1Upland hardwoodListC-Cones
Land Use 2Swamp hardwoodListKQ-Kick/Qualitative
Land Use 3ListMQM-Maine Qualitative Method
Land Use 4ListEXP-Experimental
TerrainRollingListEK-Eckman
Canopy CoverOpenListSB-Surber
Location 1Below DamListHD-Hester-Dendy
Location 2ListMH-Multihabitat
Location 3ListUNK-Not Designated
Potential Stressor(s)Land Use types
Stressor 1Regulated FlowsListUrban
Stressor 2ListCultivated
Stressor 3ListPasture
Stressor 4ListUpland hardwood
Upland conifer
Physical Characteristics of BottomSwamp hardwood
Bedrock%Swamp conifer
Boulders (>10”)20%Marsh
Rubble/Cobble (2.5” – 10”)69%Rural residential
Gravel (1/8” – 2.5”)10%
Sand (
Maine Department of Environmental Protection
Biological Monitoring Program
Aquatic Life Classification Attainment Report
Station Information
Station Number: S-1051 River Basin: Maine Coastal
Waterbody: Union River - Station 1051 HUC8 Name: Maine Coastal
Town: Ellsworth Latitude: 44 35 22.72 N
Directions: 100 METERS BELOW THE DAM. Longitude: 68 26 28.28 W
Stream Order: 4
Sample Information
Log Number: 2785
Subsample Factor: X1
Type of Sample: ROCK BAG
Replicates: 3
Date Deployed: 8/9/2019
Date Retrieved: 9/6/2019
Classification Attainment
Statutory Class:
Model Result with P>0.6: NA
Date Last Calculated: 1/21/2020
Final Determination: NA
Reason for Determination: Model
Comments:
Date: 1/21/2020
Model Probabilities
First Stage Model C or Better ModelClass A 0.00 Class C 0.66 Class A, B, or C 0.20Class B 0.00 NA 0.33 Non-Attainment 0.80
B or Better Model A ModelClass A or B 0.00 Class A 0.00
Class C or Non-Attainment 1.00 Class B or C or Non-Attainment 1.00
Model Variables
01 Total Mean Abundance 1182.00 18 Relative Abundance Ephemeroptera 0.0102 Generic Richness 21.00 19 EPT Generic Richness 10.0003 Plecoptera Mean Abundance 0.00 21 Sum of Abundances: Dicrotendipes, 0.0004 Ephemeroptera Mean Abundance 12.33 Micropsectra, Parachironomus, Helobdella
05 Shannon-Wiener Generic Diversity 2.42 23 Relative Generic Richness- Plecoptera 0.00
06 Hilsenhoff Biotic Index 5.85 25 Sum of Abundances: Cheumatopsyche, 532.16
07 Relative Abundance - Chironomidae 0.05 Cricotopus, Tanytarsus, Ablabesmyia
08 Relative Generic Richness Diptera 0.29 26 Sum of Abundances: Acroneuria, 4.00
09 Hydropsyche Abundance 0.00 Maccaffertium, Stenonema
11 Cheumatopsyche Abundance 500.83 28 EP Generic Richness/14 0.21
12 EPT Generic Richness/ Diptera 1.67 30 Presence of Class A Indicator Taxa/7 0.00
Generic Richness Five Most Dominant Taxa13 Relative Abundance - Oligochaeta 0.00 Rank Taxon Name Percent15 Perlidae Mean Abundance (Family 0.00 1 Cheumatopsyche 42.37Functional Group) 2 Neureclipsis 28.93
16 Tanypodinae Mean Abundance 4.00 3 Polycentropus 9.95(Family Functional Group) 4 Planariidae 5.75
17 Chironomini Abundance (Family 3.00 5 Oecetis 2.88Functional Group)
Report Printed: 1/21/2020 Contact: biome@maine.gov or (207)287-7688 Page 1
EXHIBIT 13
Maine Department of Environmental Protection
Biological Monitoring Program
Aquatic Life Classification Attainment Report
Station Number: S-1051 Town: Ellsworth
Log Number: 2785 Waterbody: Union River - Station 1051
Date Deployed: 8/9/2019
Date Retrieved: 9/6/2019
Sample Collection and Processing Information
Sampling Organization: PAUL LEEPER (MOODY Taxonomist: PAUL LEEPER (MOODY MOUNTAINMOUNTAIN ENVIRONMENTAL) ENVIRONMENTAL)
Waterbody Information - Deployment
Temperature:
Dissolved Oxygen:
Dissolved Oxygen Saturation:
Specific Conductance:
Velocity:
pH:
Wetted Width:
Bankfull Width:
Depth:
23.6 deg C
8.2 mg/1
12 cm/s
Waterbody Information - Retrieval
Water Chemistry
Summary of Habitat Characteristics
Landuse Name Canopy Cover Terrain
Swamp Hardwood Open Rolling
Upland Hardwood
Potential Stressor Location Substrate
Regulated Flows Below Dam Boulder 20 %
Clay 10 %
Gravel 10 %
Rubble/Cobble 69 %
Landcover Summary - 2004 Data
Sample Comments
ATTACHED ALGAE
Report Printed: 1/21/2020 Contact: biome@maine.gov or (207)287-7688 Page 2
EXHIBIT 13
•et4rE oFt00
tiff EpV1 Y~ Maine Department of Environmental Protection
Biological Monitoring Program
Aquatic Life Taxonomic Inventory Report
Station Number: S-1051
Log Number: 2785
Waterbody: Union River - Station 1051
Subsample Factor: X1 Replicates: 3
Town: Ellsworth
Calculated: 1/21/2020
Taxon
MaineTaxonomicCode
Count(Mean of Samplers)
HilsenhoffBioticIndex
FunctionalFeeding
RelativeAbundance %
Actual Adjusted Group Actual Adjusted
Planariidae 03010101 68.00 68.00 5.8 5.8
Hyalella 09010203006 6.67 6.67 8 CG 0.6 0.6
Odonata 090203 0.33 0.33 0.0 0.0
Heptageniidae 09020402 7.67 7.67 0.6 0.6
Stenonema 09020402016 4.00 4.00 4 SC 0.3 0.3
Tricmythodes 09020411038 0.67 0.67 4 CG 0.1 0.1
Chimarra 09020601003 18.33 18.33 2 CF 1.6 1.6
Neureclipsis 09020603008 342.00 342.00 7 CF 28.9 28.9
Polycentropus 09020603010 117.67 117.67 6 PR 10.0 10.0
Hydropsychidae 09020604 79.33 6.7
Cheumatopsyche 09020604015 422.67 500.83 5 CF 35.8 42.4
Macrostemum 09020604018 6.33 7.50 3 CF 0.5 0.6
Agraylea 09020607024 0.33 0.33 8 P 0.0 0.0
Oecetis 09020618078 34.00 34.00 8 PR 2.9 2.9
Thienemannimyia 09021011020 4.00 4.00 3 PR 0.3 0.3
Cricotopus 09021011037 31.33 31.33 7 SH 2.7 2.7
Eukiefferiella 09021011041 6.33 6.33 8 CG 0.5 0.5
Nanocladius 09021011049 3.67 3.67 3 CG 0.3 0.3
Rheotanytarsus 09021011072 16.00 16.00 6 CF 1.4 1.4
Polypedilum 09021011102 3.00 3.00 6 SH 0.3 0.3
Hydrobiidae 10010104 9.33 9.33 0.8 0.8
Gyraulus 10010203029 0.33 0.33 SC 0.0 0.0
Report Printed: 1/21/2020 Contact: biome@maine.gov or (207)287-7688 Page 3
EXHIBIT 13
EXHIBIT 13
From: Paul LeeperTo: Maloney, KellySubject: Fwd: Graham Lake bug dataDate: Thursday, April 16, 2020 2:39:57 PMAttachments: pre-EDD Stream_MI_Field Graham L CG-1 8-15-2019.xls
pre-EDD Stream_MI_Field Graham L CG-2 8-15-2019.xlspre-EDD Stream_MI_Field Graham L S-1 8-15-2019.xlspre-EDD Stream_MI_Field Graham L S-2 8-15-2019.xlspre-EDD Stream_MI_Field Graham L W-1 8-15-2019.xlspre-EDD Stream_MI_Field Graham L W-2 8-15-2019.xlsPre-EDD_MI_Taxonomist Graham L CG-1 8-15-2019.xlsxPre-EDD_MI_Taxonomist Graham L CG-1 9-19-2019.xlsxPre-EDD_MI_Taxonomist Graham L CG-2 8-15-2019.xlsxPre-EDD_MI_Taxonomist Graham L CG-2 9-19-2019.xlsxPre-EDD_MI_Taxonomist Graham L S-1 8-15-2019.xlsxPre-EDD_MI_Taxonomist Graham L S-1 9-19-2019.xlsxPre-EDD_MI_Taxonomist Graham L S-2 8-15-2019.xlsxPre-EDD_MI_Taxonomist Graham L S-2 9-19-2019.xlsxPre-EDD_MI_Taxonomist Graham L W-1 8-15-2019.xlsxPre-EDD_MI_Taxonomist Graham L W-1 9-19-2019.xlsxPre-EDD_MI_Taxonomist Graham L W-2 8-15-2019.xlsxPre-EDD_MI_Taxonomist Graham L W-2 9-19-2019.xlsxGraham Lake Macroinvertebrate Sites 2019.pdf
CAUTION: This email originated from outside of the organization. Do not click on links or open attachments unlessyou recognize content is safe. Please report suspicious emails here ATTENTION: Ce courriel provient d'une source externe, ne cliquez pas sur les liens et n'ouvrez pas les pièces jointes,à moins que vous en reconnaissiez la source. Veuillez nous aviser ici de tout courriel suspect.
-------- Forwarded Message --------Subject:Graham Lake bug data
Date:Tue, 10 Mar 2020 07:40:13 -0400From:Paul Leeper
To:DiFranco, Jeanne L CC:Dunlap, Frank
Morning Jeanne,
Frank Dunlap asked me to send over the bug data files for Graham Lake (2019) and AtteanPond (Normandeau 2009). Here are the files for Graham Lake as well as a map showing sitelocations. Attean data will be over later today.
If you have any questions please contact me.
Paul
-- Paul C. LeeperMoody Mountain Env.137 Diamond Str.Searsmont, ME 04973
EXHIBIT 13
mailto:moodymtn@tidewater.netmailto:Kelly.Maloney@brookfieldrenewable.commailto:phishing@brookfieldrenewable.commailto:hameconnage@energiebrookfield.commailto:moodymtn@tidewater.netmailto:Jeanne.L.DiFranco@maine.govmailto:frank.dunlap@brookfieldrenewable.comRead Me
Guidance for Completing Biomonitoring Stream Macroinvertebrate Field Data Entry FormLocationPotential Stressor
OptionsNotesAgricultural Runoff
Updated 2/18/2016Above Road CrossingAltered Habitat
General Notes:Below Road CrossingAltered Hydrology
When beginning to enter field data from a new field season, request a new version of this file from the data manager. Prior to entering all field data, load one completed test file to make sure everything works correctly.Above TownAltered Hydrology (Beaver Activity)
A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.Below TownBelow Ski Area
B) Please complete peach-colored fields and do not alter white fields.Above Fish HatcheryBelow Wetland
C) Please do not enter text into empty fields; this will interfere with data processing.Below Fish HatcheryBOD (Low DO)
D) We encourage you to fax us (207/287-7826) original field sheets so we can carry out additional QC.Above WetlandChlorine
E) Please do not change the name of the worksheet 'Stream_MI' as this will interfere with data processing. If you are submitting data for multiple sites, please do so using separate files.Below WetlandGravel Pit
Above POTWImpounded
Note that for all items below only the points that need explanation are annotated.Below POTWInorganic Solids
Above LandfillLake Outlet
1) Site/Sample event info block (first block)Below LandfillLogging
a) Waterbody Name and Town: add the relevant information; exact form or accuracy are not required - these fields are not used for loading data.Below AirportLow DO
b) Station Number: add the 1-3 digit biomonitoring station number without the 'S-' (e.g., 5 or 826); if number is unknown, leave field blank.Below In-Place ContaminationLow Gradient
c) Sampled By and Sample Method: select from drop-down list.Above In-Place ContaminationLow pH
d) Note that Deployment and Retrieval Time come from the Habitat Characteristics section on the paper field sheet.Above Point SourceMetals
e) Deployed Depth Rep 1/2/3: enter the stream depths at the locations where samplers 1-3 were deployed. If an average depth was noted on the field sheet, enter the same value three times. Note that this information also goes in field B48.Below Point SourceNPS Pollution
f) The following info does not get captured in this form:- River Basin, Stream Order - DEP staff will determine these- Directions, Lat/Long coordinates - for new site, enter info in the New Site Information worksheet - Any field sheet comment about 'number of jars' - omitf) The following info does not get captured in this form:- River Basin, Stream Order - DEP staff will determine these- Directions, Lat/Long coordinates - for new site, enter info in the New Site Information worksheet - Any field sheet comment about 'number of jars' - omitAbove Urban NPSNutrients
Below Urban NPSOrganic Solids
2) Physical Characteristics blockAbove Agriculture NPSPesticides
a) Land Use and Location: select as many options from the drop-down list as desired. Do not enter the same option multiple times.Below Agriculture NPSRegulated Flows
b) Location: see columns C and D on this worksheet for a list of options and explanations for some of them.Above Forestry NPSSedimentation
c) Use the Comments fields in Row 62 to make additional notes on Land Use or Location if necessary.Below Forestry NPSSuperfund Site
Above DamOnly used for man-made dams; note info on beaver dam in Comments field, if desired.Thermal
3) Potential Stressor(s) blockBelow DamTidal/Estuary
a) Enter as many options from the drop-down list as desired. Do not enter the same option multiple times.ImpoundmentOnly used for man-made impoundments.Toxic Organics
a) See column F on this worksheet for a list of options.Lake OutletUrban Runoff
c) Use the Comments fields in Row 62 to make additional notes on Potential Stressor(s) if necessary.Main StemOnly used for larger systems.Wetland Headwaters
Above Confluence
4) Physical Characteristics of Bottom blockBelow Confluence
a) Enter percentage values only for the substrate types found; do not enter '0' for types not found.Below Falls
b) Make sure the summed percentage is 100%.Pristine Landscape
Designated Ecoreserve
5) Habitat Characteristics blockNational Park
a) Wetted and Bankfull Width, and Depth: enter a single value, not a range. If a range (or multiple values) was noted on the field sheet, enter the average here and if desired note what the range was in the Comments field in row 62.Minimally Disturbed
b) Make sure the values entered for DO, DO Percent Saturation, Temperature, and SPC match the units supplied. If necessary, convert values e.g. from degrees Fahrenheit to degrees Celsius.
c) Meter # information: the existing set-up assumes that DO and temperature are measured with one meter and SPC/pH with another. If your set-up differs, please make sure to note the meter ID in fields B/F 54 and 56.
d) HETL Folder #: this information is not stored in the database but only entered here as an electronic record.
e) If velocity on the field sheet says "visible" leave velocity value blank. Add a comment in the comment section(s) below: "Visible flow at deployment." or "Visible flow at retrieval." If both deployment and retrieval had only visible flow, put a comment in each comment field.
6) Comments at Deployment and Comments at Retrieval
a) Enter here any relevant comments (i.e. those that could help with data interpretation) that are noted on the field form, including in the Observations block. End comment with a period.
i) If a comment is of a general nature (i.e. is not date-related, e.g. additional information on Location), it can be put in either field A62 or E62.
ii) If a comment relates only to one date, enter it in the relevant Comment field. If the wording of the comment does not include a reference to either deployment or retrieval date (e.g. 'High water on deployment.'), add the date in the comment (e.g. 7/8/11: high water).
&CPage &P of &N
Stream_MI
EDD for Rivers/Streams Macroinvertebrate Field DataLUP values for pull-downs
Waterbody NameGraham lakeSampled By
TownWalthamBiomonitoring Unit
Station NumberCG-1Number only (omit ‘S-‘)Acadia National Park
Log NumberHoulton Band of Maliseet Indians
Sampled ByMoody Mountain EnvironmentalListLotic, Inc.
Sample MethodSB-SurberListMoody Mountain Environmental
Deployment Date8/15/19Retrieval Date8/15/19Passamaquoddy Tribe
Deployment Time11:28 AMRetrieval Time11:28 AMPenobscot Indian Nation
Number Deployed3Number Retrieved3Portland Water District
Deployed Depth Rep 146cm-required
Deployed Depth Rep 246cm-required
Deployed Depth Rep 346cm-requiredSample method
RBG-Rock Bag
Physical CharacteristicsRB-Rock Basket
Land Use 1Upland hardwoodListC-Cones
Land Use 2Upland coniferListKQ-Kick/Qualitative
Land Use 3ListMQM-Maine Qualitative Method
Land Use 4ListEXP-Experimental
TerrainFlatListEK-Eckman
Canopy CoverOpenListSB-Surber
Location 1ImpoundmentListHD-Hester-Dendy
Location 2ListMH-Multihabitat
Location 3ListUNK-Not Designated
Potential Stressor(s)Land Use types
Stressor 1ImpoundedListUrban
Stressor 2ListCultivated
Stressor 3ListPasture
Stressor 4ListUpland hardwood
Upland conifer
Physical Characteristics of BottomSwamp hardwood
Bedrock%Swamp conifer
Boulders (>10”)5%Marsh
Rubble/Cobble (2.5” – 10”)60%Rural residential
Gravel (1/8” – 2.5”)25%
Sand (10”)%Marsh
Rubble/Cobble (2.5” – 10”)%Rural residential
Gravel (1/8” – 2.5”)%
Sand (
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EXHIBIT 13
From: Paul LeeperTo: Maloney, KellySubject: Fwd: Attean Pond bug dataDate: Thursday, April 16, 2020 2:41:47 PMAttachments: pre-EDD Stream_MI_Field Attean Pond ATT-1 8-24-08.xls
pre-EDD Stream_MI_Field Attean Pond ATT-2 8-24-08.xlspre-EDD Stream_MI_Field Attean Pond ATT-3 8-24-08.xlspre-EDD Stream_MI_Field Attean Pond ATT-4 8-24-08.xlspre-EDD Stream_MI_Field Attean Pond ATT-5 8-24-08.xlspre-EDD Stream_MI_Field Attean Pond ATT-6 8-24-08.xlsPre-EDD_MI_Taxonomist Attean 1-2008.xlsxPre-EDD_MI_Taxonomist Attean 2-2008.xlsxPre-EDD_MI_Taxonomist Attean 3-2008.xlsxPre-EDD_MI_Taxonomist Attean 4-2008.xlsxPre-EDD_MI_Taxonomist Attean 5-2008.xlsxPre-EDD_MI_Taxonomist Attean 6-2008.xlsxAtteanMacroinvertebrateSites2008Normandeau.pdf
CAUTION: This email originated from outside of the organization. Do not click on links or open attachments unlessyou recognize content is safe. Please report suspicious emails here ATTENTION: Ce courriel provient d'une source externe, ne cliquez pas sur les liens et n'ouvrez pas les pièces jointes,à moins que vous en reconnaissiez la source. Veuillez nous aviser ici de tout courriel suspect.
-------- Forwarded Message --------Subject:Attean Pond bug data
Date:Tue, 10 Mar 2020 09:27:04 -0400From:Paul Leeper
To:DiFranco, Jeanne L CC:Dunlap, Frank
Morning again Jeanne,
Please find enclosed bug data files for 2008 sampling done on Attean Pond by NormandeauAssociates. The data was gleaned from the following report:
Brassua Hydroelectric Project (FERC No. 2615)Macroinvertebrate SurveyInitial Study ReportPrepared forOwners of Brassua DamPrepared byNormandeau Associates, Inc.February 2008
A few notes regarding the data files.
Biomonitoring Stream Macroinvertebrate Field Data Entry Forms: I didn't have a lot of datato input but did have substrate info. I couldn't find GPS coordinates but have included a mapwith locations noted.
EXHIBIT 13
mailto:moodymtn@tidewater.netmailto:Kelly.Maloney@brookfieldrenewable.commailto:phishing@brookfieldrenewable.commailto:hameconnage@energiebrookfield.commailto:moodymtn@tidewater.netmailto:Jeanne.L.DiFranco@maine.govmailto:frank.dunlap@brookfieldrenewable.comRead Me
Guidance for Completing Biomonitoring Stream Macroinvertebrate Field Data Entry FormLocationPotential Stressor
OptionsNotesAgricultural Runoff
Updated 2/18/2016Above Road CrossingAltered Habitat
General Notes:Below Road CrossingAltered Hydrology
When beginning to enter field data from a new field season, request a new version of this file from the data manager. Prior to entering all field data, load one completed test file to make sure everything works correctly.Above TownAltered Hydrology (Beaver Activity)
A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.A) Let Susanne Meidel or the data manager or know if you need additional options in items with a pull-down list (indicated by an adjacent yellow field 'List') or more/different fields under an item with multiple options.Below TownBelow Ski Area
B) Please complete peach-colored fields and do not alter white fields.Above Fish HatcheryBelow Wetland
C) Please do not enter text into empty fields; this will interfere with data processing.Below Fish HatcheryBOD (Low DO)
D) We encourage you to fax us (207/287-7826) original field sheets so we can carry out additional QC.Above WetlandChlorine
E) Please do not change the name of the worksheet 'Stream_MI' as this will interfere with data processing. If you are submitting data for multiple sites, please do so using separate files.Below WetlandGravel Pit
Above POTWImpounded
Note that for all items below only the points that need explanation are annotated.Below POTWInorganic Solids
Above LandfillLake Outlet
1) Site/Sample event info block (first block)Below LandfillLogging
a) Waterbody Name and Town: add the relevant information; exact form or accuracy are not required - these fields are not used for loading data.Below AirportLow DO
b) Station Number: add the 1-3 digit biomonitoring station number without the 'S-' (e.g., 5 or 826); if number is unknown, leave field blank.Below In-Place ContaminationLow Gradient
c) Sampled By and Sample Method: select from drop-down list.Above In-Place ContaminationLow pH
d) Note that Deployment and Retrieval Time come from the Habitat Characteristics section on the paper field sheet.Above Point SourceMetals
e) Deployed Depth Rep 1/2/3: enter the stream depths at the locations where samplers 1-3 were deployed. If an average depth was noted on the field sheet, enter the same value three times. Note that this information also goes in field B48.Below Point SourceNPS Pollution
f) The following info does not get captured in this form:- River Basin, Stream Order - DEP staff will determine these- Directions, Lat/Long coordinates - for new site, enter info in the New Site Information worksheet - Any field sheet comment about 'number of jars' - omitf) The following info does not get captured in this form:- River Basin, Stream Order - DEP staff will determine these- Directions, Lat/Long coordinates - for new site, enter info in the New Site Information worksheet - Any field sheet comment about 'number of jars' - omitAbove Urban NPSNutrients
Below Urban NPSOrganic Solids
2) Physical Characteristics blockAbove Agriculture NPSPesticides
a) Land Use and Location: select as many options from the drop-down list as desired. Do not enter the same option multiple times.Below Agriculture NPSRegulated Flows
b) Location: see columns C and D on this worksheet for a list of options and explanations for some of them.Above Forestry NPSSedimentation
c) Use the Comments fields in Row 62 to make additional notes on Land Use or Location if necessary.Below Forestry NPSSuperfund Site
Above DamOnly used for man-made dams; note info on beaver dam in Comments field, if desired.Thermal
3) Potential Stressor(s) blockBelow DamTidal/Estuary
a) Enter as many options from the drop-down list as desired. Do not enter the same option multiple times.ImpoundmentOnly used for man-made impoundments.Toxic Organics
a) See column F on this worksheet for a list of options.Lake OutletUrban Runoff
c) Use the Comments fields in Row 62 to make additional notes on Potential Stressor(s) if necessary.Main StemOnly used for larger systems.Wetland Headwaters
Above Confluence
4) Physical Characteristics of Bottom blockBelow Confluence
a) Enter percentage values only for the substrate types found; do not enter '0' for types not found.Below Falls
b) Make sure the summed percentage is 100%.Pristine Landscape
Designated Ecoreserve
5) Habitat Characteristics blockNational Park
a) Wetted and Bankfull Width, and Depth: enter a single value, not a range. If a range (or multiple values) was noted on the field sheet, enter the average here and if desired note what the range was in the Comments field in row 62.Minimally Disturbed
b) Make sure the values entered for DO, DO Percent Saturation, Temperature, and SPC match the units supplied. If necessary, convert values e.g. from degrees Fahrenheit to degrees Celsius.
c) Meter # information: the existing set-up assumes that DO and temperature are measured with one meter and SPC/pH with another. If your set-up differs, please make sure to note the meter ID in fields B/F 54 and 56.
d) HETL Folder #: this information is not stored in the database but only entered here as an electronic record.
e) If velocity on the field sheet says "visible" leave velocity value blank. Add a comment in the comment section(s) below: "Visible flow at deployment." or "Visible flow at retrieval." If both deployment and retrieval had only visible flow, put a comment in each comment field.
6) Comments at Deployment and Comments at Retrieval
a) Enter here any relevant comments (i.e. those that could help with data interpretation) that are noted on the field form, including in the Observations block. End comment with a period.
i) If a comment is of a general nature (i.e. is not date-related, e.g. additional information on Location), it can be put in either field A62 or E62.
ii) If a comment relates only to one date, enter it in the relevant Comment field. If the wording of the comment does not include a reference to either deployment or retrieval date (e.g. 'High water on deployment.'), add the date in the comment (e.g. 7/8/11: high water).
&CPage &P of &N
Stream_MI
EDD for Rivers/Streams Macroinvertebrate Field DataLUP values for pull-downs
Waterbody NameAttean PondSampled By
TownAtteanBiomonitoring Unit
Station NumberAtt-1Number only (omit ‘S-‘)Acadia National Park
Log NumberHoulton Band of Maliseet Indians
Sampled ByListLotic, Inc.
Sample MethodKQ-Kick/QualitativeListMoody Mountain Environmental
Deployment Date8/24/08Retrieval Date8/24/08Passamaquoddy Tribe
Deployment Time1:20 PMRetrieval Time1:20 PMPenobscot Indian Nation
Number Deployed3Number Retrieved3Portland Water District
Deployed Depth Rep 1cm-required
Deployed Depth Rep 2cm-required
Deployed Depth Rep 3cm-requiredSample method
RBG-Rock Bag
Physical CharacteristicsRB-Rock Basket
Land Use 1ListC-Cones
Land Use 2ListKQ-Kick/Qualitative
Land Use 3ListMQM-Maine Qualitative Method
Land Use 4ListEXP-Experimental
TerrainListEK-Eckman
Canopy CoverListSB-Surber
Location 1ListHD-Hester-Dendy
Location 2ListMH-Multihabitat
Location 3ListUNK-Not Designated
Potential Stressor(s)Land Use types
Stressor 1ListUrban
Stressor 2ListCultivated
Stressor 3ListPasture
Stressor 4ListUpland hardwood
Upland conifer
Physical Characteristics of BottomSwamp hardwood
Bedrock%Swamp conifer
Boulders (>10”)%Marsh
Rubble/Cobble (2.5” – 10”)%Rural residential
Gravel (1/8” – 2.5”)%
Sand (10”)90%Marsh
Rubble/Cobble (2.5” – 10”)10%Rural residential
Gravel (1/8” – 2.5”)%
Sand (10”)%Marsh
Rubble/Cobble (2.5” – 10”)90%Rural residential
Gravel (1/8” – 2.5”)%
Sand (
INITIAL STUDY REPORT MACROINVERTEBRATE SURVEY
Brassua Project Owners of Brassua Dam FERC No. 2615 19 February 2009
Figure 4.1-2. Attean Pond macroinvertebrate and mussel sampling stations in 2008.
Taxa ID worksheets: Data Input methodology
1. I copied the raw data to a new sheet
2. I took out extraneous rows and columns to allow data to line up with template and thencopied the data into the template
3. I, conservatively, adjusted the taxa names to conform with the DEP taxa list, mainlyremoving "sp." from the end of genus names and "grp" from chiros.
4. You will notice some taxa do not have codes, I left them as is.
If you have any questions please contact me.
Paul
Paul C. LeeperMoody Mountain Env.137 Diamond Str.Searsmont, ME 04973
On 3/10/2020 7:40 AM, Paul Leeper wrote:
Morning Jeanne,
Frank Dunlap asked me to send over the bug data files for Graham Lake (2019)and Attean Pond (Normandeau 2009). Here are the files for Graham Lake as wellas a map showing site locations. Attean data will be over later today.
If you have any questions please contact me.
Paul
EXHIBIT 13
EXHIBIT 13
EXHIBIT 13
EXHIBIT 13
CERTIFICATE OF SERVICE I hereby certify that I have provided a copy via First Class Mail or electronic mail of the
Initial Study Report for the Brassua Hydroelectric Project to each entity designated on the
attached Distribution List.
Dated at Richmond, VA this 5th day of February, 2009. _________________________ Wendy Bley Long View Associates 2416 Mountainbrook Dr. Richmond, Virginia 23233
EXHIBIT 13
Brassua Project (FERC No. 2615) Distribution List Office of Project Review Advisory Council on Historic Pres. The Old Post Office 1100 Pennsylvania Ave NW Ste 809 Washington, DC 20004-2501 Jay Clement US Army Corps of Engineers 675 Western Avenue #3 Manchester, ME 04351 Kevin Mendik US Department of the Interior National Park Service 15 State Street 10th Floor Boston, MA 02109-3572 Michael Bartlett US Fish and Wildlife Service 70 Commercial Street Suite 300 Concord NH 03301-5087 Fred Seavey US Fish and Wildlife Service 1168 Main Street Old Town, ME 04468 Ralph Abele US Environmental Protection Agency 1 Congress St Ste 1100 (CWQ) Boston, MA 02114 Greg Stewart US Geological Survey 196 Whitten Road Augusta, ME 04333
Dana Murch Maine Department of Environmental Protection Bureau of Land and Water Quality 17 State House Station Augusta, ME 04333-0017 Christina Stacey Maine Land Use Regulation Commission Greenville Regional Office – Somerset County 43 Lakeview Street PO Box 1107 Greenville, Maine 04441-1107 Amy Hudnor Maine Department of Conservation Bureau of Parks and Lands 22 State House Station Augusta, ME 04333-0022 Tim Obrey Maine Department of Inland Fisheries & Wildlife Greenville Regional Headquarters PO Box 551 Greenville, ME 04441 Douglas Kane Maine Department of Inland Fisheries & Wildlife Greenville Regional Headquarters PO Box 551 Greenville, ME 04441 Jeff Bagley Maine Department of Inland Fisheries & Wildlife Greenville Regional Headquarters P.O. Box 551 Grenville, ME 04401
EXHIBIT 13
Dr. Arthur Spiess Maine Historic Preservation Commission 55 Captiol Street 65 State House Station Augusta, ME 04330-0065 Ian Buines Maine State Planning Office 189 State Street 38 State House Station Augusta, ME 04330-0038 Steve Timpano Maine Department of Inland Fisheries & Wildlife 41 State House Station 284 State Street Augusta, ME 04333-0041 Earle Shettleworth Maine Historic Preservation Commission 55 Capitol Street 65 State House Station Augusta, ME 04333 Marcia Spencer-Famous Maine Department of Conservation Land Use Regulation Commission 22 State House Station Augusta, ME 04333-0022 William Phillips, Chief Aroostook Band of Micmacs 7 Northern Road Presque Isle, ME 04769 William Nicholas, Governor Passamaquoddy Tribe - Indian Township Box 301 Princeton, ME 04668
David Lombard Houlton Band of Maliseet Indians Natural Resources Department 88 Bell Road Littleton, ME 04730 Bonnie Newsom, THPO Cultural and Historic Preservation Program Natural Resources Department Penobscot Indian Nation 12 Wabanaki Way Indian Island, ME 04468 John Simko Greenville Town Manager P.O. Box 1109 Greenville, ME 04441 Robert Dunphy Somerset County Commissioner District #1 41 Court Street Skowhegan, ME 04976 Sean McCormack Maine Council of Trout Unlimited P.O. Box 3862 Portland, ME 04104 Kevin Colburn American Whitewater National Stewardship Director 1035 Van Buren St. Missoula, MT 59802 Ken Kimball Appalachian Mountain Club PO Box 296 Route 16 Gorham, NH 03581
EXHIBIT 13
John Burrows Atlantic Salmon Federation Fort Andross, suite 308 14 Main Street Brunswick, ME 04011 Chad Clark Merimil Limited Partnership 160 Capital Street Augusta, ME 04330 Frank Dunlap FPL Energy Maine Hydro, LLC 160 Capital Street, Suite 8 Augusta, ME 04330 David Lovley Madison Paper Industries PO Box 129, 3 Main Street Madison, Maine 04950-0129 Laura Wildman Director, Northeast Field Office American Rivers Northeast Field Office 20 Bayberry Road Glastonbury, CT 06033 Natural Resource Consulting Services 167 South St Concord, NH 03391 FERC New York Regional Office 19 W 34th St, Rm 400 New York, NY 10001 Somerset, County of Countyhouse Skowhegan, ME 04976
Maine Office of the Attorney Office State House Station 6 Augusta, ME 04330 Maine Office of the Governor State House, Station 112 Augusta, ME 04330 Leon F. Szeptycki Environmental Counsel Trout Unlimited 1300 17th St N Ste 500 Arlington, VA 22209 Christopher Allen General Manager FPLE Maine Generation 677 Cousins St Yarmouth, ME 04096 F Allen Willey Vice President FPL Energy Maine Hydro LLC 160 Capital St, Suite 8 Augusta, ME 04330 Sarah E. Tomalty Senior Attorney FPL Energy, Inc 801 Pennsylvania Ave., NW Suite 220 Washington, DC 20004
EXHIBIT 13
Brassua Hydroelectric Project (FERC No. 2615)
Initial Study Report
Volume I of V
Prepared for Owners of Brassua Dam
Prepared by
Long View Associates, Inc.
February 2009
EXHIBIT 13
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EXHIBIT 13
INITIAL STUDY REPORT
TABLE OF CONTENTS Volume I Section I - Introduction 1 Overview ............................................................................................................................... I-1
1.1. Project Description............................................................................................................I-2
1.2. Project Location and Area ................................................................................................I-2
2 Current Project Operations ................................................................................................ I-5
3 Process and Schedule Overview ......................................................................................... I-8
3.1. FERC Determination and Study Plan Modification .........................................................I-8
3.2. Study Reporting Timeline through Updated Study Report Meeting ................................I-8
4 Summary List of Studies ..................................................................................................... I-9
5 Continuation of Studies ..................................................................................................... I-10
6 References........................................................................................................................... I-10
List of Tables Table 4-1. List of relicensing studies conducted by the Licensees. ..........................................I-9 List of Figures Figure 1.2-1. Project Location. ......................................................................................................I-3 Figure 1.2-2. Brassua Reservoir. ...................................................................................................I-4 Figure 2-1. Brassua Project Reservoir levels..............................................................................I-7 Section II - Initial Study Reports Brassua Reservoir Impoundment Fluctuation Study Littoral Habitat Survey Brassua Reservoir Bathymetric Study Water Quality Monitoring Study Fisheries Survey Brassua Reservoir - Moose River Fish Community Survey Moose River Brook Trout and Landlocked Salmon Radio-Telemetry Study Moose River Smelt Drift Study Moose River Wild Salmon Production Evaluation Moose River Salmonid Redd Survey Upper Moose River Brook Trout Fishery Assessment
Brassua Project Owners of Brassua Dam FERC No. 2615 i February 2009
EXHIBIT 13
INITIAL STUDY REPORT
Fish Entrainment and Impingement Assessment Moose River Instream Flow and Habitat Assessment Moose River Evaluation of Tailwater Fishery Enhancements Brassua Reservoir Tributary Habitat and Access Study Volume II Section II - Initial Study Reports Macroinvertebrate Survey Brassua Reservoir Aquatic Resources Survey Volume III Section II - Initial Study Reports Terrestrial Resources Study
Terrestrial Resources Survey Aquatic Furbearer Study Volume IV Section II - Initial Study Reports Mercury Sampling for Fish and Wildlife Shoreline Ownership and Development Inventory Project Redevelopment Study Project Operations Overview Desktop Review of Moose River Upstream Fish Passage Recreational Resources Inventory Section III - ISR Appendices Appendix A: FERC-Approved Study Plan Appendix B: Study Consultation Record Volume V - Privileged Cultural Resources Survey
Brassua Project Owners of Brassua Dam FERC No. 2615 ii February 2009
EXHIBIT 13
EXHIBIT 13
From: Maloney, KellyTo: Howatt, Kathy; "Livesay, Nick"; Dorman, RandySubject: Ellsworth Final BMI ReportsDate: Friday, January 31, 2020 11:14:00 AMAttachments: Graham Lake Final Macro Report-1-30-20.pdf
Union River_Macroinverts_Final 1-30-20.pdfimage002.png
Hi, Nick and Kathy, Ahead of our meeting next Thursday, please find attached the final BMI reports for Graham Lake and the UnionRiver. We are pleasantly surprised to report that Paul Leeper is of the opinion the Graham Lake meets aquatic lifedesignated use standards. We are disappointed but not at all surprised that his analysis of the river resulted inaffirmation of the previous analysis showing non-attainment. That said, there are many aspects of our currentrelicensing proposal that we think are worth revisiting in terms of potential remediation and we look forward todiscussing those with you. Thank you, Kelly MaloneyManager, Compliance – Northeast Brookfield Renewable150 Main Street, Lewiston, Maine 04240T 207-755-5606 C 207-233-1995 F 207-755-5655Kelly.Maloney@BrookfieldRenewable.comwww.brookfieldrenewable.com
EXHIBIT 13
mailto:Kelly.Maloney@brookfieldrenewable.commailto:Kathy.Howatt@maine.govmailto:Nick.Livesay@maine.govmailto:Randy.Dorman@brookfieldrenewable.commailto:Kelly.Maloney@BrookfieldRenewable.comhttp://www.brookfieldrenewable.com/2019
Graham Lake
Macroinvertebrate Community Study
Ellsworth Maine
FERC No.2727
Submitted by:
Paul C. Leeper
Moody Mountain Environmental
137 Diamond Str
Searsmont Maine 04973
Submitted to:
Frank Dunlap
Brookfield Renewable
150 Main Street
Lewiston, Maine 04240
Date: January 30, 2020
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
1.0 INTRODUCTION
This is a report on a 2019 Benthic Macroinvertebrate Survey in Graham Lake. This
survey was conducted in Graham Lake in support of the relicensing of the Ellsworth
Hydroelectric Project, Federal Energy Regulatory Commission (FERC) No. 2727. The Graham
Lake Dam impounds the approximately 10,000-acre Graham Lake at a normal maximum surface
elevation of 104.2 feet msl. Graham Lake is operated as a water storage facility where water is
stored for later use in supplementing downstream generation at the Ellsworth Dam. There are no
generating facilities at the Graham Lake Dam. The current project license requires the water
level in Graham Lake to be maintained between 93.4 and 104.2 feet msl. The Graham Lake
Development generally follows an informal target operating curve where the impoundment is
drawn down during the summer and winter and refilled in the fall and spring. According to the
operating curve, Graham Lake is drawn down from a target elevation of approximately 102 feet
msl on January 1 to a target elevation of 93.4 feet msl on March 31. Graham Lake is then filled
to a target elevation of 104.2 feet msl between April 1 and mid-May, and is gradually drawn
down over the summer to a target elevation of 97.8 feet msl by early October. Graham Lake is
then partially refilled from mid-October to the end of December to a target elevation of
approximately 102 feet msl.
Graham Lake is classified GPA. Under 38 MRSA §464(9), as a hydropower project
impoundment, the aquatic life must, at a minimum, attain Class C aquatic life standards. Class C
waters may cause some changes to aquatic life, provided the receiving waters shall be of
sufficient quality to support all species of fish indigenous to the receiving waters and maintain
the structure and function of the resident biological community. This study concentrated on the
resident biological community, namely aquatic invertebrates.
2.0 GOALS
The goals of this study were to:
1. Survey the aquatic macroinvertebrates in the shallow littoral (nearshore) habitats
in Graham Lake and describe the communities and habitat;
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
2. Make a determination on whether the aquatic life in Graham Lake, as shown by
the nearshore macroinvertebrate communities, is meeting its Class GPA standard.
3.0 STUDY AREA
The study area consisted of the approximately 10,000 acres of Graham Lake in the towns
of Ellsworth, Fletchers Landing, Waltham, and Mariaville (Figure 1). The tributaries, including
the East and West Branches of the Union River were not included in the study.
4.0 METHODS
This study followed previous Maine lake and impoundment study methodologies (Leeper
1998 and Normandeau 2009). Benthic macroinvertebrates (aquatic insects and non-insects) were
sampled at six locations in Graham Lake (Figure 1). Sampling targeted three (3) general
substrates, coarse substrates (cobble-gravel), fine substrates (sand), and wetlands (aquatic bed,
soft muds and aquatic vegetation). A Surber sampler was used to collect samples from cobble-
gravel and sand substrates. These substrates had little or no aquatic vascular plants and occurred
in areas generally exposed to wind, wave and ice action. Aquatic sweep samples were collected
from soft silt-mud substrates in protected areas which contained aquatic vascular plant growth.
Sampling took place on August 15 and September 19, 2019 under typical reservoir level
conditions.
Macroinvertebrate sampling in cobble-gravel substrates was conducted at two (2)
locations, Cobble Gravel-1 and Cobble Gravel-2 (Figure 1). The two (2) sand substrate locations
are denoted Sand-1 and Sand-2 in Figure 1. Three replicate quadrat samples were collected at
each location using a square foot Surber sampler that was placed on the substrate in water depths
between 0.5 and 2 feet.
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Figure 1. Graham Lake and the location of nearshore aquatic macroinvertebrate community sample sites in
2019.
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
A grain scoop was used to excavate the substrate within the sampler to a depth of about 5
inches. The contents of the scoops were placed in the Surber collection bag. The remaining
substrate was agitated and then the Surber was retrieved. The contents of each replicate Surber
sample was washed in a 595 µm sieve bucket to remove fine grained material, placed in a
labeled sample jar, and preserved with denatured alcohol for laboratory processing.
Two (2) wetland locations, Aq. Bed-1 and Aq. Bed-2 are shown in Figure 1. Three
replicate samples were collected at each wetland location using an aquatic D-frame sweep net.
Each replicate was collected by dragging the sweep net in a straight line for three feet with the
handle held perpendicular to the bottom. The bottom of the net penetrated the substrate by
approximately one-half inch. The contents of each replicate sweep net sample were washed in a
595 µm sieve bucket to remove fine grained material, placed in a labeled sample jar, and
preserved with denatured alcohol for laboratory processing.
All samples were transported to the Moody Mountain Environmental laboratory where
they were sorted, identified, and enumerated. At each station, mean values of three replicate
samples were used to calculate the following metrics:
Total Abundance – Total Abundance is the mean of the total number of specimens
collected from a sampling station and gear type on a given date.
Community Density – Community Density (no./m²) was only calculated from
quantitative samples, therefore density estimates were only calculated from Surber samples in
cobble-gravel and sand substrates.
Taxa Richness – Taxa richness is the number of distinct taxa (types of organisms) in a
sample and provides an estimate of the size of a macroinvertebrate community. A relatively
large number of taxa would indicate less environmental stress.
Shannon-Weiner Diversity Index – This metric compares the distribution of individuals
among all taxa present in a sample. The Shannon-Wiener diversity index (H) is a measure of
diversity that combines species richness (the number of species in a given area) and their relative
abundances. Maximum diversity is obtained when the numbers of individuals are equally
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
distributed among the taxa. A value near 0 would indicate a large number of individuals
representing one or a few species. High values indicate that the numbers of individuals are
evenly distributed among several species.
EPT Richness – Three groups of benthic insects are considered particularly sensitive to
pollution. EPT Richness is the number of distinct taxa (types of organisms) in a sample that
represents the groups (taxonomic Orders) of Ephemeroptera (mayflies), Plecoptera (stoneflies),
and Trichoptera (caddisflies), which are collectively referred to as the EPT taxa. Low values for
this metric indicate potentially stressful environmental conditions; values for this metric
generally increase with increasing water quality.
Midge Abundance – Midge (Diptera: Chironomidae) taxa are generally more tolerant of
stressful environmental conditions. Values for this metric generally increase with decreasing
water quality.
Percent Dominant Taxa – This metric uses the abundance of the most commonly
collected taxon as a measure of community balance. A community dominated by one or
relatively few taxa suggests environmental stress. If a dominant taxon accounts for a large
percentage of the individuals present, it is an indication of stress because the community is
dominated by one taxon, whereas unstressed communities typically exhibit a more evenly
balanced abundance among several taxa.
In addition to characterizing the Graham Lake macroinvertebrate community, this study
compared that community to the community in Attean Pond. Attean Pond is a 2,745 acre lake
located in the Moose River drainage. It is a natural, unregulated lake with no shoreline
development and excellent water quality. Normandeau (2009) previously studied the shallow,
near shore macroinvertebrate community using similar methods. The data set from August 2008
sampling in cobble-gravel substrates and soft silt-mud substrates in protected areas which
contained aquatic vascular plant growth were compared to the August Graham Lake community.
It should be noted that the Aquatic Life standard for Graham Lake allows for some changes in
aquatic life as long as the structure and function of resident biological community is maintained.
Therefore, the Graham Lake community does not have to be exactly like that of a natural lake,
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
but the basic structure and function must be maintained. The metrics described above were
compared between the lake communities as well an additional metric:
Community Loss Index – The community loss index measures the loss of benthic taxa
in samples from an experimental station (Graham Lake) compared to those found at the reference
station (Courtemanch and Davies 1987). It also factors in the replacement of missing taxa with
different taxa. The value of this index can range from 0 to infinity, and increases as the test
station becomes increasingly dissimilar to the reference station; therefore, lower values indicate
less stressful conditions.
5.0 RESULTS
Physical habitat characteristics of each sampling station are provided in Table 1.
Photographs of the sites are included in Appendix 1. Aquatic sweep samples were collected
from sites Aq. Bed-1 and -2 (Figure 1). Overall, these sites had a substrate that was
predominately composed of soft muds, clay, and detritus (organic matter). Submerged aquatic
vegetation (SAV) and floating aquatic vegetation was abundant at both sites. Surber samples
were collected from sites Cobble Gravel-1 and -2. As named, the substrates at these sites were
predominantly cobble and gravel, although it was noted at both sites that the cobble was
embedded in clay or mud. Neither site had extensive aquatic vegetation but some attached algae
was present. Surber samples were also used at sites Sand-1 and -2. These sites were
predominantly sands with some gravel and muds. No aquatic vegetation was present at these
sites. Both the Cobble Gravel and Sand sites were located in areas that received more wave
action than the Aquatic Bed sites, which precluded the presence of silt, mud, and extensive
vegetation.
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Table 1. Substrate Composition, temperature and dissolved oxygen at littoral macroinvertebrate sampling
sites on Graham Lake, August, September 2019.
Station DateSample
TypeBoulder Cobble Gravel Sand Silt/Mud Clay Muck Detritus Temp (
°C)
Diss Oxy
(mg/l)
G-CG-1 15-Aug-19 Surber 5 60 25 10 24.9 7.8
G-CG-2 15-Aug-19 Surber 10 40 30 10 10 24 7.5
G-Snd-1 15-Aug-19 Surber 30 60 10 27 8.1
G-Snd-2 15-Aug-19 Surber 25 70 5 23.2 8
G-Wtl-1 15-Aug-19 Aq. Sweep 50 20 30 24.7 7.6
G-Wtl-2 15-Aug-19 Aq. Sweep 40 40 20 22.7 7.8
Substrate % Composition Water Quality
A complete list of site data including taxa and the number of individuals per taxon
collected from each sample is provided in Appendix 3. Summarized benthic macroinvertebrate
data are provided in Table 2. Overall a total of 7881 organisms representing 71 taxa were
collected in the study. S-W Diversity values were generally low, ranging from 1.37 to 2.50.
Sensitive mayflies and caddisflies (EPT) taxa made up 30% of the total taxa collected. The
wetland habitats were the most numerous, by an order of magnitude, and rich communities.
Sand and cobble-gravel habitats were much less numerous and had 15% and 35% less taxa
respectively. As mentioned above, overall diversity values for these communities were generally
low. The sand communities had the highest values overall. Sensitive EPT taxa were well
represented in all communities and made up over a third of the taxa at sand and cobble-gravel
sites. Mean total abundance of EPT rose in all habitats from August to September. Midge
larvae, generally more adaptable to stressful environmental conditions, were a minor component
of the communities reaching a high of 15% of the abundance in sand habitat in August.
However, the midges Tanytarsus and Tribelos were dominant taxa in sand habitat in August and
September respectively. The aquatic snail, Amnicola sp. and the amphipod Hyalella azteca were
dominant taxa (>5%) in all habitats in both months. Combined these two (2) taxa represented
between 21% and 72% of the communities across habitats and months. Sensitive mayflies
Stenacron and Eurylophella were major components of the cobble gravel communities and the
wetland communities in September.
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Table 2. Summarized metrics from the nearshore aquatic macroinvertebrate communities in Graham Lake,
2019.
Tot. Ab. % Ab Tot. Ab. % Ab
Cob/Gr 33.83 364.18 22 1.39 1.94 11 7.33 21% 1.67 5%
Sand 69.00 742.71 27 1.68 2.04 7 5.33 8% 10.17 15%
Wetl 684.17 44 1.76 2.24 5 34.17 5% 46.00 7%
Cob/Gr 43.67 470.02 21 1.91 2.15 7 9.67 38% 1.67 7%
Sand 41.53 447.02 26 1.97 2.50 8 15.33 37% 4.83 12%
Wetl 529.60 24 1.37 1.58 5 78.00 15% 1.20 0%
Cob/Gr 38.75 417.10 32 1.39 2.15 13
Sand 55.25 594.71 42 1.68 2.50 13
Wetl 603.88 49 1.37 2.15 5
EPT MidgeSample
Site
August Sampling
September Sampling
Totals by Substrate
Mean Tot.
Abund.
Density
Ind./sq.
meter
Taxa
Richness
S-W Div.
Minimum
S-W Div.
Maximum
Mayfly,
Stonefly,
Caddisfly
(EPT)
Richness
Table 2b. Dominant taxa in nearshore aquatic macroinvertebrate communities in Graham Lake, 2019.
Amnicola 39% Hyalella azteca 23% Amnicola 34% Eurylophella 16% Amnicola 38% Hyalella azteca 52%
Oligochaeta 19% Eurylophella 21% Oligochaeta 32% Oligochaeta 14% Hyalella azteca 34% Amnicola 15%
Hyalella azteca 13% Oligochaeta 19% Tanytarsus 7% Hyalella azteca 11% Cladocera 6% Eurylophella 13%
Stenacron 12% Amnicola 12% Hyalella azteca 6% Tribelos 10% Cladocera 7%
Stenacron 6% Amnicola 10% Enallagma 5%
Polycentropus 6% Planariidae 6%
August
Cobble/ Gravel Sand Wetland
August SeptSeptAugustSept
A comparison of communities in Graham Lake to communities in Attean Pond, an
unregulated, natural Great Pond is shown in Table 3. Data from cobble-gravel and wetland
communities were compared from the month of August. This comparison presents a mixed
picture. Overall the Graham Lake community is as abundant, or more so, than the Attean
community and as diverse. However, the Graham Lake community has fewer taxa and the most
dominant taxa represent a greater proportion of the community.
Comparisons of the wetland communities shows total abundance is greater at Graham
Lake, taxa richness is similar, as is diversity, indicating little difference between the
communities. However, the most dominant taxon represents over a third of the community and
sensitive EPT taxa are less represented. The Community Loss Index value (0.81) indicates some
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
change in the Graham Lake community and some missing taxa are replaced with different taxa.
This is within the range of Community Loss Index values that Courtemanch and Davies (1987)
found indicated water segments essentially unaffected by human use.
Comparisons of the cobble-gravel communities shows total abundance and diversity to be
similar and sensitive EPT taxa are represented similarly. But taxa richness is depressed at
Graham and the most dominant taxon represents over a third of the community. The Community
Loss Index value (1.23) indicates a loss of taxa at Graham Lake and there has been little
replacement of those taxa with different ones. This indicates a greater change to the Graham
Lake community.
Table 3. Comparisons of community metrics between 2019 Graham Lake nearshore aquatic
macroinvertebrates and those of Attean Pond in 2008.
Metric Attean Pond Graham Lake Comments
Total Abundance 290.6 684.2 Graham moderately different
Taxa Richness 54 43 minor depression of richness indicated
Percent Dominant Taxon 27% 38% minor adverse effects indicated
EPT Richness 10 5 some adverse effects indicated
Shannon Diversity 2.00 1.88 no adverse effects indicated
Community Loss Index
some change in Graham community some
missing taxa are replaced
Metric Attean Pond Graham Lake Comments
Total Abundance 26.8 34.17
no adverse effects indicated, moderate
increase at Graham
Density (No./m²) 291.1 367.77
no adverse effects indicated, moderate
increase at Graham
Taxa Richness 35 22 adverse effects indicated at Graham
Percent Dominant Taxon 23% 39% some adverse effects indicated at Graham
EPT Richness 10 11 no adverse effects indicated
Shannon Diversity 2.20 1.97 no adverse effects indicated
Community Loss Index community loss at Graham, less taxa replaced
August Aquatic Sweep Samples- wetland (SAV mud) substrate
August Surber Samples- cobble-gravel substrates
0.81
1.23
6.0 DISCUSSION
The goal of this study is to make a determination on whether the aquatic life in Graham
Lake, as shown by the nearshore macroinvertebrate communities, is meeting its Class GPA
standard. Graham Lake Dam is classified GPA and under 38 MRSA §464(9), as a hydropower
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
project impoundment, the aquatic life must, at a minimum, attain Class C aquatic life standards.
Class C waters may cause some changes to aquatic life, provided the waters maintain the
structure and function of the resident biological community, in this case, the near shore aquatic
invertebrate community. The data show that the communities in the typical habitats in Graham
Lake are rich in taxa, and organisms sensitive to environmental stress are present, and in some
cases, abundant in the communities. Overall the communities were dominated by common and
ubiquitous lake organisms, namely Amnicola snails and the amphipod Hyalella azteca. Hyalella
azteca is "the most abundant amphipod of lakes [in North America]" (Mason 2002).
Comparisons with communities from a natural unregulated lake, show some changes.
Wetland communities in Graham Lake are similar to communities in Attean Pond but are
different enough that Community Loss values were indicative of stressed conditions. Cobble-
gravel communities in Graham Lake show greater change when compared to Attean
communities. The most notable difference is that taxa richness at Graham is over 30% less than
at Attean. The Community Loss values reflect this and indicate greater change or stress to the
community.
Based on this data, it is my professional opinion that the structure and function of the
resident biological community is maintained in Graham Lake. The community is dominated by
common lake organisms and sensitive organisms are well represented in all typical habitats.
Comparisons of community metrics from Graham Lake to the unregulated Attean Pond show the
communities are different, especially the cobble gravel communities. However, the structure and
function of the community in Graham Lake aquatic communities is still maintained.
7.0 SUMMARY
1. The objectives of this study were to survey the aquatic macroinvertebrates in the
shallow littoral (nearshore) habitats in Graham Lake and describe the communities
and habitat and to;
2. Make a determination on whether the aquatic life in Graham Lake, as shown by the
nearshore macroinvertebrate communities, is meeting its Class GPA standard.
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
3. Previous Maine lake and impoundment study methodologies were followed in this
study. Sampling targeted three (3) general substrates, coarse substrates (cobble-
gravel), fine substrates (sand), and wetlands (aquatic bed, soft muds and aquatic
vegetation). Surber samplers were used on cobble-gravel and sand substrates, aquatic
sweep samples were collected from wetland (soft silt-mud substrates). Sampling took
place on August 15 and September 19, 2019 under typical reservoir level conditions.
4. The results show that the communities in the typical habitats in Graham Lake are rich
in taxa, and organisms sensitive to environmental stress are present, and in some
cases, abundant in the communities. Overall the communities were dominated by
common and ubiquitous lake organisms, namely Amnicola snails and the amphipod
Hyalella azteca.
5. Comparisons to the communities in Attean pond, a natural, unregulated lake show
differences in the communities. Wetland communities were more similar to Attean
communities and metrics indicated some stressed conditions at Graham Lake.
Cobble-gravel communities were less similar to Attean and metrics indicated more
stressed conditions at Graham Lake.
6. Based on these results it is my professional opinion that the structure and function of
the resident biological community is maintained in Graham Lake. The community is
dominated by common lake organisms and sensitive organisms are well represented
in all typical habitats. Comparisons of community metrics from Graham Lake to the
unregulated Attean Pond show changes to the Graham Lake communities. However,
the structure and function of the community in Graham Lake is still maintained.
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Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Literature Cited
Courtemanch, D.L. and S.P. Davies. 1987. A coefficient of community loss to assess
detrimental change in aquatic communities. Wat. Res. 21(2): 217-222.
Leeper, P.C. 1988. Quantitative comparison study of the nearshore aquatic macroinvertebrate
communities at Flagstaff Lake, Attean Pond, and Second Musquacook Lake. Eco-
Analysts Inc. Report to FPL Energy Maine Hydro LLC.
Mason C. F. 2002. "Acidification". Biology of Freshwater Pollution (4th ed.). Pearson
Education. pp. 175–204.
Normandeau Associates, Inc. 2009. Brassua hydroelectric project (FERC No. 2615)
macroinvertebrate survey initial study report. Prepared for Owners of Brassua Dam.
Appendix 1- Macroinvertebrate Site Photos
Appendix 1--Page-1-
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Photo 1 & 2. Cobble Gravel Site 1
Appendix 1--Page-2-
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Photo 3 & 4. Cobble Gravel Site 2
Appendix 1--Page-3-
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Photo 5 & 6. Sand Site 1
Appendix 1--Page-4-
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Photo 7 & 8. Sand Site 2.
\
Appendix 1--Page-5-
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Photo 9 & 10. Aquatic Bed Site 1
Appendix 1--Page-6-
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Photo 11 & 12. Aquatic Bed Site 2
Appendix 2- Sample Site data and benthic macroinvertebrates collected in Graham Lake, August
and September, 2019.
Appendix 2--Page-1 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Waterbody Name Graham lake
Town Waltham
Station Number CG-1 Number only (omit ‘S-‘)
Log Number
Sampled By Moody Mountain Environmental List
Sample Method SB-Surber List
Deployment Date 8/15/2019 Retrieval Date 8/15/2019
Deployment Time 11:28 AM Retrieval Time 11:28 AM
Number Deployed 3 Number Retrieved 3
Deployed Depth Rep 1 46 cm-required
Deployed Depth Rep 2 46 cm-required
Deployed Depth Rep 3 46 cm-required
Physical Characteristics
Land Use 1
Upland hardwood List
Land Use 2 Upland conifer List
Land Use 3 List
Land Use 4 List
Terrain Flat List
Canopy Cover Open List
Location 1 Impoundment List
Location 2 List
Location 3 List
Potential Stressor(s)
Stressor 1 Impounded List
Stressor 2 List
Stressor 3 List
Stressor 4
List
Physical Characteristics of Bottom
Bedrock
%
Boulders (>10”) 5 %
Rubble/Cobble (2.5” – 10”) 60 %
Gravel (1/8” – 2.5”) 25 %
Sand (
Appendix 2--Page-2 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Maine Department of Environmental Protection
Logsheet for Benthic Macroinvertebrates Identified
Please see the Read Me worksheet
Taxonomist: Paul Leeper (Moody Mountain Environmental)
Sample Log No.: Chironomidae Subsample (SS) Effort
Station No.: CG-1 Level of SS Effort none none none
Waterbody Name: Graham Lake No. Chir SSed
Town: Waltham No. Chir in SS
Date of Collection: 8/15/2019 Misc. Chir not SSed
Time of Collection: 11:28 AM TChir 0 0 0
Sampled By: PCL
Subsample Factor:
Sampler Type: SB-Surber
Retrieval Depth Unit Depth 1 Depth 2 Depth 3
Taxon No. identified from sample
Maine Code Taxon Name Stage Comment Rep 1 Rep 2 Rep 3
09010203006011 Hyalella azteca 6 9 2
10010104013 Amnicola 20 48 11
0802 Oligochaeta 4 5 3
09020407 Ephemeridae 2 0 1
09020402 Heptageniidae 2 0 0
09020618 Leptoceridae 2 0 0
09021011020 Thienemannimyia 2 0 0
09021011076 Tanytarsus 0 0 1
09020402014 Stenacron 0 1 4
09020603010 Polycentropus 0 1 0
08030201 Hirudinidae 0 0 1
09020412040 Caenis 0 0 1
09020401 Baetidae 0 0 1
Total Benthos 38 64 25
Appendix 2--Page-3 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Maine Department of Environmental Protection
Logsheet for Benthic Macroinvertebrates Identified
Please see the Read Me worksheet
Taxonomist: Paul Leeper (Moody Mountain Environmental)
Sample Log No.: Chironomidae Subsample (SS) Effort
Station No.: CG-1 Level of SS Effort none none none
Waterbody Name: Graham Lake No. Chir SSed
Town: Waltham No. Chir in SS
Date of Collection: 9/19/2019 Misc. Chir not SSed
Time of Collection: 11:28 AM TChir 0 0 0
Sampled By: PCL
Subsample Factor:
Sampler Type: SB-Surber
Retrieval Depth Unit Depth 1 Depth 2 Depth 3
Taxon No. identified from sample
Maine Code Taxon Name Stage Comment Rep 1 Rep 2 Rep 3
10010104013 Amnicola 5 2 6
09010203006011 Hyalella azteca 10 12 6
0802 Oligochaeta 5 5 11
09020618077 Triaenodes 1 0 0
09020603010 Polycentropus 1 0 2
090504 Cladocera 1 0 0
09020410036 Eurylophella 6 12 22
10020201002 Pisidium 0 1 2
09020402014 Stenacron 1 1 0
03010101 Planariidae 1 1 0
09020702004 Sialis 0 1 1
09020607026 Hydroptila 0 2 0
09021011037 Cricotopus 0 0 3
09021011107 Tribelos 1 0 0
Total Benthos 32 37 53
Appendix 2--Page-4 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Waterbody Name Graham lake
Town Mariaville
Station Number CG-2 Number only (omit ‘S-‘)
Log Number
Sampled By Moody Mountain Environmental List
Sample Method SB-Surber List
Deployment Date 8/15/2019 Retrieval Date 8/15/2019
Deployment Time 2:00 PM Retrieval Time 2:00 PM
Number Deployed 3 Number Retrieved 3
Deployed Depth Rep 1 46 cm-required
Deployed Depth Rep 2 46 cm-required
Deployed Depth Rep 3 46 cm-required
Physical Characteristics
Land Use 1
Upland hardwood List
Land Use 2 Upland conifer List
Land Use 3 List
Land Use 4 List
Terrain Flat List
Canopy Cover Open List
Location 1 Impoundment List
Location 2 List
Location 3 List
Potential Stressor(s)
Stressor 1 Impounded List
Stressor 2 List
Stressor 3 List
Stressor 4
List
Physical Characteristics of Bottom
Bedrock
%
Boulders (>10”) 10 %
Rubble/Cobble (2.5” – 10”) 40 %
Gravel (1/8” – 2.5”) 30 %
Sand (
Appendix 2--Page-5 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Maine Department of Environmental Protection
Logsheet for Benthic Macroinvertebrates Identified
Please see the Read Me worksheet
Taxonomist: Paul Leeper (Moody Mountain Environmental)
Sample Log No.: Chironomidae Subsample (SS) Effort
Station No.: CG-2 Level of SS Effort none none none
Waterbody Name: Graham Lake No. Chir SSed
Town: Waltham No. Chir in SS
Date of Collection: 8/15/2019 Misc. Chir not SSed
Time of Collection: 11:28 AM TChir 0 0 0
Sampled By: PCL
Subsample Factor:
Sampler Type: SB-Surber
Retrieval Depth Unit Depth 1 Depth 2 Depth 3
Taxon No. identified from sample
Maine Code Taxon Name Stage Comment Rep 1 Rep 2 Rep 3
09010203006011 Hyalella azteca 1 5 3
10010104013 Amnicola 1 0 0
0802 Oligochaeta 3 15 9
09020604015 Cheumatopsyche 1 1 0
10020201002 Pisidium 0 1 0
09020412039 Brachycercus 0 1 0
09020401 Baetidae 0 1 0
090504 Cladocera 0 0 4
09020402014 Stenacron 5 5 10
09020603010 Polycentropus 1 0 2
09020607 Hydroptilidae 0 0 1
09020607026 Hydroptila 0 0 1
09021011107 Tribelos 0 1 1
09021011037 Cricotopus 0 1 1
09021011082 Cryptochironomus 1 0 0
Total Benthos 13 31 32
Appendix 2--Page-6 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Maine Department of Environmental Protection
Logsheet for Benthic Macroinvertebrates Identified
Please see the Read Me worksheet
Taxonomist: Paul Leeper (Moody Mountain Environmental)
Sample Log No.: Chironomidae Subsample (SS) Effort
Station No.: CG-2 Level of SS Effort none none none
Waterbody Name: Graham Lake No. Chir SSed
Town: Waltham No. Chir in SS
Date of Collection: 9/19/2019 Misc. Chir not SSed
Time of Collection: 11:28 AM TChir 0 0 0
Sampled By: PCL
Subsample Factor:
Sampler Type: SB-Surber
Retrieval Depth Unit Depth 1 Depth 2 Depth 3
Taxon No. identified from sample
Maine Code Taxon Name Stage Comment Rep 1 Rep 2 Rep 3
10010104013 Amnicola 11 6 2
09010203006011 Hyalella azteca 28 5 0
0802 Oligochaeta 15 11 3
09020603010 Polycentropus 2 9 1
09030103 Hydrachnidae 1 0 0
09020402014 Stenacron 4 3 6
09020406 Leptophlebiidae 1 4 2
09020410036 Eurylophella 7 3 4
10020201002 Pisidium 0 0 1
09021012 Simuliidae 0 0 1
09021011037 Cricotopus 0 3 2
09021011102 Polypedilum 1 0 0
09021011 Chironomidae 0 1 0
09021011020 Thienemannimyia 1 0 0
09021011097 Parachironomus 1 0 0
09021011107 Tribelos 1 0 0
Total Benthos 73 45 22
Appendix 2--Page-7 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Waterbody Name Graham lake
Town Fletcher's Landing
Station Number S-1 Number only (omit ‘S-‘)
Log Number
Sampled By Moody Mountain Environmental List
Sample Method SB-Surber List
Deployment Date 8/15/2019 Retrieval Date 8/15/2019
Deployment Time 1:00 PM Retrieval Time 1:00 PM
Number Deployed 3 Number Retrieved 3
Deployed Depth Rep 1 46 cm-required
Deployed Depth Rep 2 46 cm-required
Deployed Depth Rep 3 46 cm-required
Physical Characteristics
Land Use 1
Upland hardwood List
Land Use 2 Upland conifer List
Land Use 3 List
Land Use 4 List
Terrain Flat List
Canopy Cover Open List
Location 1 Impoundment List
Location 2 List
Location 3 List
Potential Stressor(s)
Stressor 1 Impounded List
Stressor 2 List
Stressor 3 List
Stressor 4
List
Physical Characteristics of Bottom
Bedrock
%
Boulders (>10”) %
Rubble/Cobble (2.5” – 10”) %
Gravel (1/8” – 2.5”) 30 %
Sand (
Appendix 2--Page-8 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Maine Department of Environmental Protection
Logsheet for Benthic Macroinvertebrates Identified
Please see the Read Me worksheet
Taxonomist: Paul Leeper (Moody Mountain Environmental)
Sample Log No.: Chironomidae Subsample (SS) Effort
Station No.: S-1 Level of SS Effort none none none
Waterbody Name: Graham Lake No. Chir SSed
Town: Waltham No. Chir in SS
Date of Collection: 8/15/2019 Misc. Chir not SSed
Time of Collection: 1:00 PM TChir 0 0 0
Sampled By: PCL
Subsample Factor:
Sampler Type: SB-Surber
Retrieval Depth Unit Depth 1 Depth 2 Depth 3
Taxon No. identified from sample
Maine Code Taxon Name Stage Comment Rep 1 Rep 2 Rep 3
10010104013 Amnicola 20 22 28
0802 Oligochaeta 9 9 4
09010203006011 Hyalella azteca 3 1 6
03010101 Planariidae 1 1 0
09020412040 Caenis 4 0 2
09020607028 Oxyethira 1 0 1
09020603010 Polycentropus 1 0 0
09020618075 Mystacides 5 0 2
09020615069 Molanna 2 0 3
08030201 Hirudinidae 0 1 0
10010102 Viviparidae 0 2 1
09030103 Hydrachnidae 0 0 1
09021011107 Tribelos 3 1 1
09021011020 Thienemannimyia 3 1 1
09021011082 Cryptochironomus 0 1 1
09021011076 Tanytarsus 18 7 3
09021011001 Ablabesmyia 0 0 2
09021011097 Parachirononmus 1 0 0
Total Benthos 71 46 56
Appendix 2--Page-9 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Maine Department of Environmental Protection
Logsheet for Benthic Macroinvertebrates Identified
Please see the Read Me worksheet
Taxonomist: Paul Leeper (Moody Mountain Environmental)
Sample Log No.: Chironomidae Subsample (SS) Effort
Station No.: S-1 Level of SS Effort none none none
Waterbody Name: Graham Lake No. Chir SSed
Town: Waltham No. Chir in SS
Date of Collection: 9/19/2019 Misc. Chir not SSed
Time of Collection: 7:50 AM TChir 0 0 0
Sampled By: PCL
Subsample Factor:
Sampler Type: SB-Surber
Retrieval Depth Unit Depth 1 Depth 2 Depth 3
Taxon No. identified from sample
Maine Code Taxon Name Stage Comment Rep 1 Rep 2 Rep 3
10010104013 Amnicola 8 0 11
09010203006011 Hyalella azteca 7 16 4
0802 Oligochaeta 9 16 7
09021010043 Bezzia/palpomyia 1 1 1
10010104 Hydrobiidae 1 0 2
09020603010 Polycentropus 1 4 2
09020402014 Stenacron 4 2 4
03010101 Planariidae 4 4 8
09020410036 Eurylophella 12 7 17
09020607026 Hydroptila 1 0 1
09020406 Leptophlebiidae 4 5 2
09020618078 Oecetis 8 6 0
10020201002 Pisidium 0 1 0
08030201 Hirudinidae 0 1 0
09020309 Coenagrionidae 0 1 0
09021014052 Chrysops 0 1 0
09021007 Chaoboridae 0 0 1
090504 Cladocera 0 0 1
09020406025 Leptophlebia 0 0 1
09021011107 Tribelos 13 6 7
09021011076 Tanytarsus 0 1 0
09021011057 Rheocricotopus 0 1 009021011037 Cricotopus 1 0 0
Total Benthos 74 73 69
Appendix 2--Page-10 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Waterbody Name Graham lake
Town Fletchers Landing
Station Number S-2 Number only (omit ‘S-‘)
Log Number
Sampled By Moody Mountain Environmental List
Sample Method SB-Surber List
Deployment Date 8/15/2019 Retrieval Date 8/15/2019
Deployment Time 7:50 AM Retrieval Time 7:50 AM
Number Deployed 3 Number Retrieved 3
Deployed Depth Rep 1 40 cm-required
Deployed Depth Rep 2 40 cm-required
Deployed Depth Rep 3 40 cm-required
Physical Characteristics
Land Use 1
Upland hardwood List
Land Use 2 Upland conifer List
Land Use 3 List
Land Use 4 List
Terrain Flat List
Canopy Cover Open List
Location 1 Impoundment List
Location 2 List
Location 3 List
Potential Stressor(s)
Stressor 1 Impounded List
Stressor 2 List
Stressor 3 List
Stressor 4
List
Physical Characteristics of Bottom
Bedrock
%
Boulders (>10”) %
Rubble/Cobble (2.5” – 10”) %
Gravel (1/8” – 2.5”) 25 %
Sand (
Appendix 2--Page-11 -
Moody Mountain Environmental 137 Diamond Str Searsmont ME 04973 ph.207-592-8540 moodymtn@tidewater.net
Maine Department of Environmental Protection
Logsheet for Benthic Macroinvertebrates Iden
Recommended