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Rochester Institute of Technology Rochester Institute of Technology
RIT Scholar Works RIT Scholar Works
Theses
8-11-2021
Effects of wetland design and invasive species removal on carbon Effects of wetland design and invasive species removal on carbon
and microbial communities in restored wetlands and microbial communities in restored wetlands
Benjamin Hamilton bth9544@rit.edu
Follow this and additional works at: https://scholarworks.rit.edu/theses
Recommended Citation Recommended Citation Hamilton, Benjamin, "Effects of wetland design and invasive species removal on carbon and microbial communities in restored wetlands" (2021). Thesis. Rochester Institute of Technology. Accessed from
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RIT
Effects of wetland design and invasive species removal on carbon and microbial communities in restored wetlands
By:
Benjamin Hamilton
A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of
Science in Environmental Science
Thomas H. Gosnell School of Life Sciences
College of Science
Environmental Science Program
Rochester Institute of Technology
Rochester, NY
August 11, 2021
Committee Approval:
Carmody McCalley, PhD Date Committee Member, Thesis Advisor
Anna Christina Tyler, PhD Date Committee Member
Nathan Eddingsaas, PhD Date Committee Member
Andre Hudson, PhD Date Committee Member
i
Table of Contents Acknowledgments...........................................................................................................................ii List of Tables and Figures..............................................................................................................iii Abstract...........................................................................................................................................iv Introduction......................................................................................................................................1 Methods............................................................................................................................................4 Results............................................................................................................................................11 Discussion......................................................................................................................................23 Broader Impacts/Conclusions........................................................................................................29 References......................................................................................................................................31
ii
Acknowledgements
I would like to thank my advisor Dr. Carrie McCalley. Her continued patience and support over the past several years has helped me grow immeasurably not only professionally but also personally. I would not be where I am today without her. I would also like to thank the rest of my committee, Dr. Christy Tyler, Dr. Nathan Eddingsaas, and Dr. Andre Hudson for their support and help throughout this project. I would like to thank the College of Science and Waste management as well as all the wonderful HANA volunteers for their invaluable support.
Thank you to my parents Randy and Janine Hamilton for all of their love and support throughout the years. Thank you to my wonderful fiancée Patsy Hayes for all of her support over the years. Finally, I would like to thank my fellow schoolmates that helped me so much over the course of this project, the HANA team of Delanie Spangler, Brie Burt-Stringer, Evan Squier, Sydney VanWinkle and Michael McGowan, and my fellow aquatic ecology lab members, Kristina Chomiak, Brianna Pollard, and Shrada Shrestra. Working with all of you has made the past few years incredibly fun and we have made memories that will last a lifetime. I truly miss getting to go out to the field, working, laughing, and catching animals with all of you every day. There are so many other people in my life that have supported me throughout this project that I cannot list but I would like you to know that I am eternally grateful.
iii
List of Tables and Figures
Figure 1: Map of study site..............................................................................................................5
Figure 2: Sample NMR spectrum....................................................................................................7
Figure 3: NMDS plot of microbial communities by area..............................................................14
Figure 4: Abundance of microbial phyla by area...........................................................................15
Figure 5: DOM structural components..........................................................................................17
Figure 6: CH4 and CO2 potential production.................................................................................18
Figure 7: NMDS plot of microbial communities before and after herbicide application..............21
Figure 8: Abundance of microbial phyla before and after herbicide application..........................22
Table 1: Soil chemistry values by area..........................................................................................12
Table 2: Results of ANOVA and Kruskal-Wallis on soil chemistry by area……........................13
Table 3: Results of ENVFIT tests..................................................................................................14
Table 4: Results of PERMANOVA and Kruskal-Wallis on microbial communities by area.......15
Table 5: Results from Kruskal-Wallis on DOM flourescnece.......................................................16
Table 6: DOM fluorescence index values......................................................................................16
Table 7: Soil chemistry values before and after herbicide application..........................................19
Table 8: Results of ANOVA and Kruskal-Wallis on soil chemistry before and after herbicide application……………………………………………………………………………...………...20
Table 9: Results of PERMANOVA and Kruskal-Wallis microbial communities before and after herbicide application……………………………………………………………………………..22
iv
Abstract
Multiple wetland ecosystem services such as carbon sequestration and nutrient removal are
influenced by microbial communities and dissolved organic matter (DOM). I examined DOM
composition, carbon metabolism, and microbial communities in three created wetlands, which
had distinct hydrology, vegetation and antecedent land-use. To study differences between
wetlands, porewater and soil were collected from each wetland in spring, summer, and fall of
2018. DOM was analyzed using NMR spectroscopy and fluorescence spectrometry, soil
microbial community composition was examined using 16S ribosomal sequencing, and CO2 and
CH4 production rates were measured in anaerobic soil incubations. Structural DOM composition
varied significantly between the three wetlands but did not vary seasonally. There were distinct
differences in the microbial community composition of each wetland, which correlated with soil
chemistry factors but did not correlate with CH4 or CO2 production rates. Average CH4/CO2
production ratios were approximately 1:1 for all sites in the spring and summer and approached
3:1 in the fall, with no differences between sites. This suggests that while DOM characteristics
and microbial communities in created wetlands are impacted by site characteristics, these
differences have less effect on carbon metabolism. I also examined the effects of invasive
species removal via herbicide application on microbial communities in one of the wetlands. Soil
was collected in the spring and summer before and after the application of glyphosate herbicide
and microbial communities were examined using 16S ribosomal sequencing. There were
significant and persistent changes in the microbial community following invasive species
removal through application of herbicide. Further study is needed to determine direct causal
relationships between invasive species control measures and microbial community changes, to
determine whether shifts in microbial communities persist past a single growing season and to
identify impacts of invasive removal and herbicide application on key wetland functions.
1
Introduction
Wetlands are considered to be one of the most socially valuable ecosystems in the world
in part due to their immense ability to provide ecosystem services such as carbon cycling and
carbon sequestration (Costanza et al., 2014). Despite their value, as much as 87% of natural
wetlands have been lost globally since 1700 (Davidson et al., 2014). The continental United
States has had a net gain of 3521 km2 of herbaceous wetland land cover from 2001 to 2011
(Homer et al., 2015). Given the wetland creation and replacement laws in the United States
(Federal Water Pollution Control Act, 2002) it is reasonable to assume that a significant portion
of this increase consists of man-made created wetlands. The increasing prevalence of these
ecosystems underscores the importance of understanding their unique biogeochemistry and how
wetland construction decisions impact these processes.
Inland water systems such as wetlands have been recognized for their role in carbon (C)
cycling and important contributions to the global carbon budget (Battin et al., 2009). Wetlands
contain a significant amount of dissolved organic matter (DOM) and an estimated 220 Pg of
carbon is stored in North American wetland soils (Bridgham et al., 2006). However, C cycling in
created wetlands often differs from natural wetlands, with lower rates of biomass production and
decomposition than their natural counterparts (Fennessy et al., 2008). While biomass production
removes carbon from the atmosphere, decomposition of organic matter by microbial
communities generates greenhouse gasses such as CO2 and CH4 (Battin et al., 2009; Bridgham et
al., 2006). Wetlands are generally carbon sinks but created wetlands that are designed or
managed poorly can become sources of CO2 and CH4, and created wetlands tend to have higher
net fluxes of greenhouse gases than natural wetlands (Kayranli, et al., 2010). It is therefore
important that carbon storage and greenhouse gas production are considered during wetland
2
construction and management. However, there is a wide variation in the design of created
wetlands and the effects of design decisions on microbial communities, C cycling and
greenhouse gas production are not well known.
Wetland ecosystems encompass a wide range of vegetation communities and hydrologic
regimes, resulting in considerable variation in soil and water chemistry and microbial community
structure, which can impact the rate of organic matter decomposition, nutrient removal and
greenhouse gas production. Many of these key wetland factors are partially or wholly determined
during the wetland design and construction process. However, the interactions between these
factors in determining key functions such as C storage, nutrient cycling and greenhouse gas
production are not well understood, making it difficult to identify optimal wetland design and
management. Furthermore, studies have also shown that microbial community structure and
composition are significantly different between natural and created wetlands (Ansola et al., 2014;
Arroyo et al., 2015; Cao et al., 2017) suggesting the need for studies that focus specifically on
created wetland systems.
Taken alone, the effect of individual wetland characteristics on microbial community
structure and greenhouse gas production are better understood. The design of hydrologic regimes
in created wetlands can have profound effects on biogeochemical processes in wetlands.
Permanently flooded conditions limit oxygen diffusion and develop anaerobic sediments that
promote anaerobic processes such as methanogenesis and denitrification. Conversely, in sites
that are seasonally flooded, oxygen penetration is more variable and aerobic processes can occur
during periods of low water table (Baldwin & Mitchell, 2000). Changes in hydrological regime
have also been shown to alter the structure of soil microbial communities in aquatic ecosystems,
likely due to the changes in oxygen availability (Ahn & Peralta, 2009; Foulquier et al., 2013;
3
Moche, Gutknecht, Schulz, Langer, & Rinklebe, 2015). Hydrologic regimes also have a
determinative impact on the plant communities that develop in created wetlands (Ahn & Dee,
2011). Plant communities in turn can have downstream impacts on DOM (Barber et al., 2001),
microbial community structure (Kourtev et al., 2003), and greenhouse gas production (Inglett et
al., 2012). In many ecosystems there is also evidence that there are predictable relationships
between plant and microbial community structure (Angeloni, et al., 2006; Arroyo et al., 2015;
Kourtev, et al., 2003).
Soil chemistry in created wetlands is also heavily impacted by construction decisions,
such as the land-use history of the parcel of land used. Soil nutrients and organic matter
composition is also known to be an important driver of microbial community composition (Ahn
& Peralta, 2009). For example, the prevalence of methanogenic organisms, a key microbial
functional group, is negatively correlated with the concentration of alternative electron acceptors
(S. He et al., 2015). However, many of these dynamics may have overlapping effects on
microbial community structure and their interactions require more study (Lee et al., 2019).
Understanding how differences in wetland environmental factors and management
strategies influence microbial community structure and function is important for developing
better wetland design and management practices. In pursuit of that goal this study had four
objectives. First, to evaluate if there are differences in microbial community structure between
created wetlands with different land use histories and hydrologic design. I hypothesized that each
created wetland would have a unique microbial community and that those differences would
correlate with soil chemistry and hydrology. Second, to evaluate if created wetlands with
different land use histories and hydrology have differences in the structure of their DOM. I
hypothesized that given the differences in plant community composition between wetlands there
4
would be differences in the composition and seasonality of DOM. Third, to evaluate if
differences in microbial communities and DOM resulted in differences in carbon metabolism
between the wetlands. I hypothesized that each wetland would have differences in rates of
carbon metabolism and these differences would be correlated with differences in microbial
community structure. Fourth, to evaluate whether invasive species removal through the use of
herbicide application would alter microbial community composition. I hypothesized that invasive
species control measures would have no long-term effect on microbial communities.
Methods
Study sites
This study took place in 2017 and 2018 at the High Acres Nature Area (HANA), owned
and operated by Waste Management of New York LLC, in Perinton, NY, USA (43° 5’ N, 77°
23’ W). Three created wetlands were chosen within HANA for this study, Area One North
(A1N), Area Two South (A2S), and Area Three C (A3C, Figure 1).
A1N is a shallow emergent marsh environment covering approximately 1.87 ha. A1N
was constructed on the site of a former gravel mine repository, the site was abandoned and left
fallow in the 1960s before being converted to a wetland in 2009 (Stantec Consulting, 2009).
Water levels are controlled by a culvert located in the southern end of the wetland and
consistently has standing water. The plant community in A1N is dominated predominantly by
narrow and broadleaf-arrowhead (Saggittaria, spp.), pickerelweed (Pontederia cordata), and
white pond lily (Nymphaea odorata). A2S is a seasonally flooded wetland covering
approximately 0.37 ha. A2S has seasonal standing water that recedes in the summer in periods of
low precipitation but generally retains soil moisture. A2S was excavated and constructed on the
5
site of a former agricultural field in 2009 (Stantec Consulting, 2009). At the start of the study,
spring 2017, A2S was dominated by a near monoculture of reed canary grass (Phalaris
arundinacea). Rodeo (glyphosate, C3H8NO5P) herbicide was applied in A2S on September 21st
and 22nd of 2017 as an invasive species control measure at an application rate of 1.8 pints/acre
by method of foliar spraying, killing nearly all of the vegetation. A2S remained relatively bare
for the duration of the 2018 growing season. A3C is a seasonally flooded wetland of
approximately 0.61 ha, that was constructed on a former cow pasture in 2012. A3C is seasonally
flooded and generally has standing water in the spring and fall but is dry in the summer. Water
plantain (Alisma subcordatum), rice cut grass (Leersia oryzoides), and narrow and broadleaf
cattail (Typha spp) are the dominant plant species.
Figure 1. Map of the three created wetlands used in this study. Area One North (A1N) is outlined in Blue, Area Two South (A2S) is outlined in red and Area Three C (A3C) is outlined in black.
6
Soil Collection and Porewater Collection
Eight 1x1m plots were established in each of the wetlands studied. Soil cores were taken
to a depth of ten centimeters in each of the plots in A2S in the spring, and summer of 2017, and
in each plot in all of the three wetlands in the spring, summer, and fall of 2018. Each soil core
was homogenized in the field and stored on ice for transport. A subset of each core was frozen
for nutrient and microbial analysis on the day of collection. In 2018 a subset of each core was
sieved and used for anaerobic soil incubations. Porewater was collected using lysimeters at a
depth of 10cm in each wetland in the spring, summer, and fall of 2018. Porewater was stored on
ice for transport, filtered within 12 hours and immediately frozen for later analysis on the day of
collection. Porewater was collected based on groundwater availability and therefore not collected
in every plot in every season.
DOM analysis
The fluorescent portion of the porewater DOM was analyzed using a Carey Eclipse
Fluorescence Spectrometer. Excitation of each sample was performed every five nm from 295 to
600 nm and emission was measured every five nm from 295-600 nm. The freshness index of
each sample was calculated as the emission intensity at 380 nm divided by the maximum
intensity of emission between 420 and 435 nm at the excitation of 310 nm (Parlanti, Wörz,
Geoffroy, & Lamotte, 2000; Wilson & Xenopoulos, 2009). This index provides information
about relative freshness of the organic matter, with larger values indicative of more recently
derived DOM. The Fluorescence index of each sample was calculated as the emission intensity
of wavelengths 470nm/520nm at excitation of 370 nm. This index provides information about
the source of the DOM, from terrestrially derived sources (plant and soil organic matter) having
7
a value of ~1.2 and microbially derived (bacteria and algal by-products) having a value of ~1.8
(Cory & McKnight, 2005).
A portion of each porewater sample was freeze-dried to isolate the DOM. Freeze dried
samples were analyzed using 500Mhz NMR. Four structural groups were identified in the DOM,
Aromatic, Carbohydrate, CRAM (Carboxylic rich alicyclic molecules), and Alykl groups (Figure
2). The relative proportion of each structural group, measured against a Sodium
trimethylsilylpropanesulfonate (DSS) standard, was quantified for each sample.
Figure 2. The regions of integration for each of the structural components that were examined using NMR spectroscopy, overlaid on a sample spectrum.
Soil Chemistry Analysis
Soil percent moisture was calculated by immediately drying soil from the field at 60oC
and recording the weight lost. Soil organic matter content (%OM) was calculated by the loss on
8
ignition method (Heiri et al., 2001). Inorganic phosphorus was extracted from dried soil through
dissolution in 1M HCl, total phosphorus was extracted from dried soil by adding 0.5 ml
MgNO2(3) to ~0.1g of dry soil and then ashing the soil at 550oC for 1.5 hours followed by
dissolution in 1M HCl. The dissolved phosphate levels were then measured using the ammonium
molybdate method (Murphy & Riley, 1962). Soil extractable nitrate+nitrite and ammonium were
extracted by shaking soil in 2M KCl and filtering the supernatant through a 45 um filter.
Nitrate+nitrite concentrations were analyzed using the method described by Doane and Horwath
(2003). Soil ammonium concentrations were measured using the Phenol-hypochlorite method
(Solórzano, 1969). Extractable ammonium and nitrate+nitrite were combined to obtain total
extractable N. Soil percent carbon (%C), percent nitrogen (%N) and C:N was measured using a
Perkin Elmer CNHS/O 8000 analyzer.
Soil incubations
Soil greenhouse gas production potential was evaluated by measuring the production of
CO2, and CH4 from the soil samples collected in each wetland in the spring, summer and fall of
2018. The soil from each plot was homogenized, sieved at 2.38 mm to remove rocks and roots,
placed in airtight jars with equal weight nanopure water, and flushed with N2 to establish
anaerobic conditions. An equal amount of soil from each plot was dried at 60oC to find the dry
weight of the soil added. Each jar was incubated at 22oC for an anaerobic period of 14 days to
allow the microbial community to acclimate and was then flushed with N2 to re-establish a CO2
and CH4 free headspace. Jars were then incubated at 22oC and gas samples were collected 6-8
times over the next 12-14 days. The concentration of CO2 and CH4 in gas samples was measured
using a Shimadzu-2014 gas chromatograph with a methanizer and FID detector and the change
in concentration over time per gram of dry soil was used to determine potential production rates.
9
Soil DNA Extraction and analysis
Soil microbial DNA was extracted from ~0.5g of soil from each plot sampled using
MoBio Powersoil® DNA extraction kits. DNA quality was confirmed using a nanodrop
spectrometer and frozen at -20oC until analysis. The variable V3-V4 region of the 16S bacterial
gene was amplified using the primer pair MiSeq341F (5’-
TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and
MiSeq805R (5’-
GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-
3’). Sequencing was performed on a Illumina MiSeq System, samples were loaded at a
concentration of 8pM and sequenced using the MiSeq V3 600 cycle kit for 2 x 250 bp cycles.
Microbial DNA was processed using Qiime 2 software (Bolyen et al., 2019). Microbial
Operational Taxonomic Units (OTUs) were defined for analysis as having 99% sequence
similarity. An even sampling depth of 9137 OTUs was selected to account for differences in
read numbers between extractions and exclude as few samples as possible. Taxonomy of each
sample was assigned using a naive Bayes classifier, trained with the Greengenes reference
database at 99% sequence similarity.
Statistical analysis
Statistical tests were performed using R-statistical software (R Core Team, 2017).
Normality of soil greenhouse gas production potential, DOM fluorescence indices as well as
each of the soil chemistry characteristics measured was assessed using shapiro-wilks test,
homogeneity of variance was assessed using levene's test. Data that was non normally distributed
was log transformed. Two-way ANOVA was used to analyze the effects of wetland site, season,
10
invasive species removal via herbicide application, and their interactions on the data that was
normally distributed and met the assumptions for homogeneity of variance. Tukey HSD post
hoc tests were performed where significant effects were observed using the “agricolae” package
(Mendiburo, 2019). For data that was non normally distributed but had homogeneity of variance,
Kruskal-Wallis tests were used with Dunn post hoc test and Benjamini-Hochberg P-value
adjustment.
DOM structural characteristics were split into four structural chemical groups,
“Aromatic,” “Carbohydrates,” “Carboxylic Rich Alicyclic Materials” (CRAM), and “alkyl,”
groups. These groups were then bound for each sample and differences were tested between site
and season with a two-way MANOVA test.
OTU diversity for each site and season was measured using Shannon’s diversity index
and significant differences between sites and seasons as well as treatment and season were
measured using Kruskal-Wallis test with Dunn post hoc test and Benjamini-Hochberg p-value
adjustment. Differences in microbial community structure were evaluated using the
PermANOVA test found in the adonis function of the “vegan” package in R statistical software
(Oksanen, 2010). The envfit function of the “vegan” package was used to fit soil chemistry and
CO2 and CH4 production vectors to a nonmetric multidimensional scaling ordination plot
(NMDS plot) constructed from Bray-Curtis dissimilarity distances. The significance of each
factor was evaluated using a goodness of fit statistic tested against 999 permutations of the
environmental variables (Oksanen, 2010).
11
Results
Wetland Type
Soil chemistry
Soil %OM, total phosphorus, soil moisture, soil %C, soil %N, and soil C:N differed
significantly between each site (Table 1). Soil %OM was highest in A2S and lowest in A1N. Soil
total phosphorus was similar in A2S and A3C (p=0.3), but significantly lower in A1N than A2S
(p=0.04) or A3C (p<0.001), with a significant seasonal interaction (Table 1 and 2). Soil moisture
content was higher in Spring and Fall than Summer (p=0.002, p=0.008), soil moisture in A1N
was significantly higher than A3C (p=0.013). Soil %C (p=0.001) and %N (p<0.001) differed
between wetlands, for %N, A2S=A3C>A1N, while for %C, A2S>A3C=A1N, resulting in soil
C:N ratio differences between wetlands, with A1N>A2S>A3C (Table 1 and 2). The soil
extractable N in each wetland was similar, but varied by season (p=0.041, Table 1 and 2).
12
Tabl
e 1.
Soi
l phy
sica
l che
mis
try fa
ctor
s in
each
wet
land
in e
ach
seas
on e
xpre
ssed
as m
eans
± st
anda
rd e
rror
.
A3C
Fall
12.8
4 ±
1.58
921.
2 ±
31.5
24.1
0 ±
4.26
50.1
5 ±
0.1
6
6.07
± 0
.65
0.46
± 0
.04
15.2
5 ±
0.69
Sum
mer
13.3
0 ±
1.57
1103
.2 ±
43.
1
32.3
1 ±
3.31
32.2
0 ±
2.70
4.47
± 0
.34
0.33
± 0
.02
15.6
8 ±
0.84
Sprin
g
13.6
4 ±
1.60
886.
3 ±
55.3
37.8
8 ±
8.42
45.5
0 ±
3.18
4.96
± 0
.46
0.38
± 0
.02
15.9
6 ±
0.93
A2S
Fall
16.8
6 ±
1.58
870.
9 ±
40.4
42.5
6 ±
3.34
47.9
2 ±
1.61
8.17
± 0
.69
0.55
± 0
.06
18.0
4 ±
0.81
Sum
mer
17.9
3 ±
1.08
1033
.4 ±
34.
9
33.8
6 ±
5.64
44.7
1 ±
1.90
6.15
± 0
.33
0.40
± 0
.02
17.8
8 ±
0.20
Sprin
g
15.6
6 ±
0.68
871.
6 ±
37.7
31.9
0 ±
4.67
52.4
6 ±
1.81
5.70
± 0
.46
0.40
± 0
.03
17.5
9 ±
0.19
A1N
Fall
10.4
2 ±
0.78
854.
3 ±
22.1
25.4
3 ±
3.35
50.5
6 ±
3.36
5.26
± 0
.43
0.31
± 0
.03
20.4
2 ±
1.19
Sum
mer
9.79
± 0
.53
808.
8 ±
35.3
11.6
9 ±
6.21
50.1
4 ±
2.08
5.15
± 0
.39
0.30
± 0
.03
20.6
3 ±
2.12
Sprin
g
10.2
0 ±
0.81
874.
9 ±
45.1
40.1
9 ±
3.85
50.4
0 ±
2.15
4.56
± 0
.23
0.28
± 0
.02
18.9
8 ±
0.29
Soil
Char
acte
ristic
s
Org
anic
mat
ter (
%)
Tota
l Pho
spho
rous
(mg/
kg)
Soil
extra
ctab
le N
(mg/
kg)
Soil
Moi
sture
(%)
Car
bon
(%)
Nitr
ogen
(%)
C:N
Rat
io
13
Table 2. Results of two-way ANOVA and Kruskal-Wallis tests comparing the effects of each area and season on soil chemistry characteristics. Significant p-values (<0.05) are bolded, p<0.001 are starred. Soil Characteristic Season Site Season x Site F, χ2 p F, χ2 p F p Organic matter (%) χ2
2,71=0.14 0.9 χ22,71=23.2 <0.001* - -
Total Phosphorous (mg/kg) F2,71=6.8 0.002 F2,71=7.8 <0.001* F4,71=4.3 0.004 Soil Extractable N (mg/kg) F2,71=3.4 0.04 F2,71=3.1 0.05 F4,71=3.8 0.007 Soil Moisture (%) χ2
2,71=13.1 0.001 χ22,71=8.3 0.02 - -
Carbon (%) χ22,71=6.5 0.04 χ2
2,71=13.7 0.001 - - Nitrogen (%) χ2
2,71=5.8 0.05 χ22,71=20.2 <0.001* - -
C:N Ratio χ22,71=1.2 0.5 χ2
2,71=39.8 <0.001* - -
Microbial distribution
The microbial communities clustered distinctly within each wetland and were
significantly different from each other (PERMANOVA, p = 0.001). Although less clearly
clustered, season also had a significant effect on microbial community structure across all
wetlands (PERMANOVA, p = 0.023). Soil %OM, soil moisture, soil %C, soil %N, soil C:N and
total soil phosphorus were all significantly correlated with the ordination of the microbial
communities (Figure 2, Table 5). There was no significant correlation of greenhouse gas
production with microbial community ordination. The Shannon diversity index of microbial
diversity in each wetland was significantly higher in the spring (A1N: 10.05 ± 0.25, A2S: 10.52
± 0.1, A3C: 9.14 ± 0.06, mean ± se), than the summer (A1N: 9.09 ±0.08, A2S: 8.95 ± 0.09, A3C:
8.93 ± 0.05) or the fall (A1N: 9.03 ± 0.09, A2S: 9.23 ± 0.23, A3C: 9.05 ± 0.25 ), there were no
significant differences in the Shannon diversity index between sites..
The microbial communities in all wetlands were dominated predominantly by the phyla
Proteobacteria, Bacteroidetes, Verrucomicrobia, and Chlorobi (Figure 4). A1N had a lower
portion of Proteobacteria, Planctomycetes, and Nitrospirae than A2S or A3C, and had a higher
abundance of Bacteroidetes, Firmicutes, Gemmatimonadetes, and Euryarchaeota. A2S had a
14
lower abundance of Verrucomicrobia and a higher abundance of Chlorobi than A1N or A3C.
A3C had a much lower proportion of rare phyla than A1N or A2S.
Figure 3. The NMDS ordination comparing the bacterial community structure between each of the wetlands in 2018. Stress values of the NMDS ordination are below 0.2 indicating an acceptable fit. The length of each vector on the graph is proportional to the strength of the correlation between each of the soil chemistry factors and the microbial community structure. Significant correlations (p<0.05) are starred.
Table 3. Results of ENVFIT permutation test comparing soil characteristics and greenhouse gas production potential with microbial community ordination. Soil Characteristic R2 p Organic matter (%) 0.46 <0.001* Total Phosphorus (mg/kg) 0.26 <0.001* Soil Extractable N (mg/kg) 0.05 0.193 Soil Moisture (%) 0.46 <0.001* Carbon (%) 0.22 0.002* Nitrogen (%) 0.30 <0.001* C:N Ratio 0.32 <0.001* CH4 Production <0.01 0.995 CO2 Production <0.01 0.896
15
Table 4. Results of PERMANOVA and Kruskal-Wallis comparing the effects of area and season on microbial distribution and microbial diversity. Significant p-values are bolded, p<0.001 are starred. Characteristic Season Site Season x Site F p F p F p Community structure F2,61=0.13 0.023 F2,61=6.8 <0.001* F4,61=3.4 0.071 Shannon diversity χ2
2,61=20.8 <0.001* χ22,61=5.6 0.058 - -
Figure 4. The relative abundance of each phylum taken from each wetland. All phylum that did not make up more than one percent of the abundance in any season were combined into a category denoted as rare.
DOM Results
There were no significant differences in the fluorescence index between sites or seasons
(Table 5 and 6). The freshness index was significantly higher in A1N than A2S (Table 5 and 6).
NMR analysis revealed differences in DOM structure by site (MANOVA, p=0.01), but no
significant differences in DOM structure by season (MANOVA, p=0.3, Figure 5).
16
Table 5. Results of Kruskal-Wallis comparing the effects of season and wetland on DOM fluorescence indices. Significant p-values <0.05 are bolded, p<0.001 are starred. Soil Characteristic Season Site F p F p Fluorescence index χ2
2=1.71 0.425 χ22=2.21 0.329
Freshness index χ22=2.64 0.267 χ2
2=11.26 0.004
Table 6. Fluorescence index and freshness index values expressed as averages ± standard error Fluorescence index Freshness index A1N Spring 1.49 ± 0.06 0.61 ± 0.02 Summer 1.63 ± 0.03 0.6 ± 0.01 Fall 1.54 ± 0.09 0.71 ± 0.04 A2S Spring 1.63 ± 0.06 0.57 ± 0.01 Summer 1.57 ± 0.03 0.59 ± 0.01 Fall 1.51 ± 0.03 0.57 ± 0.01 A3C Spring 1.55 ± 0.07 0.60 ± 0.03 Summer - - Fall 1.56 ± 0.06 0.62 ± 0.03
17
Figure 5. The relative proportions of structural components of DOM taken from A1N and A2S in fall of 2017, and DOM taken from each of the three created wetlands in spring of 2018, summer of 2018 and fall of 2018.
Potential Carbon Metabolism Rates
There were no significant differences in soil CO2 or CH4 production potential between
the wetlands (Figure 6). However, CO2 production potential was significantly higher in the
summer than the spring (p=0.007) and the fall (p=0.004) and CH4 production rates were
significantly higher in the fall than the spring (p<0.001 Figure 6). The CH4:CO2 production rate
ratio ranged from 0.76 mg C g soil-1 d-1 in A3C in the summer to 3.31 mg C g soil-1 d-1 in A1N in
the fall and was significantly higher in fall than the spring (p<0.001) or summer (p<0.001).
18
Figure 6. Soil CH4 and CO2 production potential values in the spring, summer, and fall. One star indicates a significant difference in production potential between seasons.
Impacts of invasive species control
Soil chemistry
There was a significant increase in soil organic matter content, and total soil phosphorus
and a decrease in soil %C in the year following invasive species control through herbicide
application (Table 7 and 8). There were also significant differences in the soil moisture content
of the soil before and after herbicide application (Table 7 and 8). Soil extractable N, and soil C:N
ratio were unchanged by invasive species control measures (Tables 7 and 8). Each soil chemistry
parameter showed significant seasonal variability except for soil C:N, soil moisture content, and
soil extractable N (Table 8).
19
Ta
ble
7. S
oil p
hysic
al c
hem
istry
fact
ors
befo
re a
nd a
fter h
erbi
cide
app
licat
ion
in e
ach
seas
on
expr
esse
d as
mea
ns ±
stan
dard
err
or.
Post
-Her
bici
de
Sum
mer
201
8
17.9
3 ±
1.08
1033
.43
±34.
8
33.8
6 ±
5.64
44.7
1 ±
1.90
6.15
± 0
.33
0.40
± 0
.02
17.8
8 ±
0.20
Sprin
g 20
18
15.2
2 ±
0.68
883.
06 ±
37.
67
32.4
0 ±
4.67
52.7
0 ±
1.81
5.85
± 0
.46
0.38
± 0
.03
17.7
4 ±
0.19
Pre-
Herb
icid
e
Sum
mer
201
7
17.2
2 ±
0.63
791.
38 ±
26.
67
17.4
2 ±
2.01
42.6
6 ±
2.24
7.40
± 0
.32
0.49
± 0
.02
17.8
7 ±
0.19
Sprin
g 20
17
12.0
1 ±
0.92
715.
67 ±
22.
47
14.2
5 ±
2.26
36.6
5 ±
1.80
6.24
± 0
.47
0.39
± 0
.03
18.8
8 ±
0.54
Soil
Char
acte
ristic
s
Org
anic
mat
ter (
%)
Tota
l Pho
spho
rous
(mg/
kg)
Soil
Extr
acta
ble
N (m
g/kg
)
Soil
Moi
stur
e (%
)
Carb
on (%
)
Nitr
ogen
(%)
C:N
Rat
io
20
Table 8. Results of two-way ANOVA and Kruskal-Wallis comparing the effects of invasive species removal via herbicide application and season on soil chemistry characteristics in Area two south. Significant p-values of <0.05 are bolded, values of p<0.001 are bolded and starred. Soil Characteristic Season Treatment Season x Treatment F, χ2 p F, χ2 p F p Organic matter (%) F1,30=18.
4 <0.001 F1,30=6.4 0.018 F1,30=3.00 0.094
Total Phosphorous (mg/kg) F1,30=13.3
0.001 F1,30=42.4
<0.001* F1,30=1.1 0.315
Soil Extractable N (mg/kg) F1,30=0.51
0.48 F1,30=17.8
<0.001* F1,30=0.18 0.675
Soil Moisture (%) χ21,30=1.7 0.192 χ2
1,30=11 <0.001* - - Carbon (%) F1,30=4.3 0.046 F1,30=6.6 0.030 F1,30=0.79 0.3793 Nitrogen (%) F1,30=4.5 0.045 F1,30=3.2 0.086 F1,30=1.46 0.238 C:N Ratio χ2
1,30=0.6 0.429 χ21,30=1.1 0.286 - -
Microbial community structure
The microbial community structure as described by the BrayCurtis dissimilarity index in
A2S underwent a significant shift after herbicides were applied to control invasive species. There
were also significant differences in the microbial community based on season and significant
interactions between season and treatment (Table 9). The structure of the microbial community
in the spring after invasive species control measures were used appears to be similar to the
community structure in the summer before herbicide was applied, and the community structure
appears to diverge further from initial conditions in the summer after herbicide application
(Figure 7). There were significant changes in the Shannon diversity score related to both season
and treatment (Table 9). The Shannon diversity score was significantly higher the year before
herbicide was applied to control invasive species (before application 10.5 ± 0.08, after
application 9.7 ± 0.23) and significantly higher in the spring than in the summer (spring 10.5 ±
0.07, summer 9.7 ± 0.21). The microbial community was dominated predominately by the phyla
Proteobacteria, Bacteroidetes, Verrucomicrobia, and Chlorobi across years and seasons.
21
Qualitative analysis of the phyla present in the two years showed that after herbicide was applied
to manage invasive species the relative abundance of organisms in the phyla of Chlorobi and
OD1 were higher and the relative abundance of organisms in the phyla of Nitrospirae,
Actinobacteria, Gemmatimonadetes, Firmicutes, and Spirochetes were lower (Figure 8).
Figure 7. shows the NMDS ordination plot of microbial communities before and after herbicides were used to control invasive species cover. Stress values of less than 0.2 indicate an acceptable fit of the NMDS plot to the original Bray-Curtis values.
22
Table 9. Results of PERMANOVA and Kruskal-Wallis comparing the effects of treatment and season on microbial community structure and diversity. Significant p-values are bolded, p<0.001 are starred. Characteristic Season Treatment Season x Treatment F, χ2 p F, χ2 p F p Community structure F1,27=1.9 0.005 F1,27=2.6 <0.001* F1,27=2.2 0.001 Shannon diversity χ2
1,27=6.1 0.01 χ21,27=5.6 0.02 - -
Figure 8. The relative abundance of each phylum taken from each wetland before and after herbicide was applied to control invasive species. All phylum that did not make up more than one percent of the abundance in any season were combined into a category denoted as rare.
23
Discussion
This study examined three created wetlands that had distinct soil chemistry, land use
history, plant communities and hydrology and found that each of these wetlands had distinct
microbial community composition, which correlated with soil chemistry but not carbon gas
production potential. There were weaker, but significant, seasonal variations in microbial
community composition in each wetland. Wetlands also had significant differences in DOM
structure, which remained consistent throughout the seasons measured. The studied wetlands had
similar carbon production potentials, with moderate production rates in the growing season
followed by a spike in production potential in the fall. The similarity in carbon production
potential between the wetlands despite differences in DOM structure suggests that the structure
of DOM is not a key driver of rates of anaerobic carbon metabolism. The similarity in soil
carbon production potential across sites despite the differences in microbial community structure
suggest that there is a functional redundancy in the microbial communities. This study also
suggests that control of invasive species using glyphosate herbicide can alter the composition of
microbial communities, however, this component of the experiment was incidental and a more
controlled experimental design is needed to confirm these patterns.
Wetland type
Soil chemistry
Soil chemistry regimes varied across the studied wetlands and these differences are likely
related to differences in land-use history and hydrologic conditions of each wetland. The
elevated organic matter and phosphorus content found in the sites with agricultural land use
histories are consistent with global patterns of soil phosphorus legacies (MacDonald et al., 2012).
24
Agricultural enrichment of soil phosphorus tends to diminish over time (MacDonald et al.,
2012), but the longevity of the elevated phosphorus levels found in this study shows that land use
history can impact created wetlands for years. Past studies of these wetlands have found
significant differences in soil extractable nitrogen (Lodge, 2017), however those differences
were not observed in this study, suggesting that the impact of past agricultural land use on soil
nitrogen levels is short lived relative to the impact on soil phosphorus levels. These results
demonstrate the long term impact of land use history on soil nutrient levels in created wetlands,
with agricultural land-use history resulting in higher nutrient levels. However, as time goes on
these effects may become less pronounced. The effects of hydrologic conditions, particularly the
ability to control water levels, permanently impacts soil moisture content.
Microbial community
Each of the studied wetlands had a distinct microbial community, however, the
permanently flooded wetland (A1N) was substantially different from the two seasonally flooded
wetlands (A2S and A3C). There were significant correlations between microbial community
structure and soil chemistry, including moisture content, organic matter content, total
phosphorus, % C, % N and C:N ratio. This reinforces the findings of other studies that showed
that soil chemistry is a key driver of microbial community structure (Ahn & Peralta, 2009;
Ansola et al., 2014; Arroyo et al., 2015; Foulquier et al., 2013). The divergence of A1N from the
other wetlands in the study does not appear to be fully explained by the differences in soil
chemistry and is likely due to it being permanently flooded whereas the other studied wetlands
were only seasonally inundated. Divergence due to soil dryness would be consistent with other
studies that have shown that even temporary soil dryness can lead to long term shifts in wetland
25
microbial communities (Fierer, et al. 2003; Foulquier et al., 2013). These shifts in the microbial
community may be related to changes in the plant communities induced by the seasonal drying,
or may be due to the introduction of atmospheric oxygen into the soil.
DOM and Carbon Metabolism
The DOM in each of the studied wetlands, as measured by the fluorescence index (Cory
& McKnight, 2005), was derived approximately evenly from terrestrial and microbial sources
and the source did not vary seasonally. This suggests that the differences in plant communities
and potential differences in runoff patterns into each of the wetlands did not strongly influence
DOM composition. These wetlands likely have similar inputs of DOM from runoff due to their
geographic proximity; potential regional differences in DOM composition in runoff merits more
study. There were differences in the freshness index between wetlands, indicating different
degrees of degradation of the DOM (Wilson & Xenopoulos, 2009). These changes in DOM
freshness did not correlate with differences in carbon production potential suggesting that the
degree of degradation of DOM is not driving this portion of microbial community function in
created wetlands. Differences in DOM structure between wetlands were likely related to the
differences in vegetation and past land use between each of the wetlands as shown by previous
studies (Barber et al., 2001; Graeber et al., 2012). However, there were no significant seasonal
changes in DOM structure. This contradicts previous studies of stream systems that found
changes in the chemical composition of DOM seasonally (Neff et al., 2006), but supports the
finding of other studies that found that DOM structure does not shift seasonally (Graeber et al.,
2012). Despite the lack of seasonal changes in DOM structure, there were seasonal changes in
the microbial community structure and carbon production potential, suggesting that factors other
26
than DOM structure are driving community structure and carbon production in created wetlands.
It is possible that non-structural components of DOM such as molecular weight are more
important to microbial utilization and breakdown rates (Amon & Benner, 1996).
There were no differences in greenhouse gas production potential between wetlands,
however, there was a significant increase in CO2 production potential in the summer and CH4
production potential in the fall. This increase contradicts the findings of previous studies in
ponds that showed higher CH4 production potential in the spring than the summer or fall
(Stadmark & Leonardson, 2007). However it is consistent with field studies that show an
increase in methane emissions in the fall in wetland systems (Liikanen et al., 2006; Xu et al.,
2014). The increase in methane production in the fall is likely due to an influx in labile organic
matter due to plant senescence. It is unclear why the CO2 production potential was higher in the
summer and this phenomenon merits more study. The similarity in greenhouse gas production
potential between the wetlands was surprising and contradicts the results of other studies.
Previous studies have shown significant differences in CO2 and CH4 production potential due to
differences in plant communities (Inglett, et al., 2012), but this was not observed in the studied
wetlands, despite stark differences in plant community composition. Previous studies have also
found positive correlations between CO2 production potential and soil organic matter content (Y.
He et al., 2016), but the observed differences in organic content between our wetlands did not
yield corresponding changes in carbon gas production rates.
Hydrology is also an important driver of greenhouse gas production in created wetlands
(Mander et al., 2011), however, the incubation approach used in this study did not allow for the
direct testing of the effects of water levels or soil moisture on greenhouse gas production. I was
able to examine the potential of the soil microbial communities to produce greenhouse gasses
27
under ideal conditions. My findings that the differences in microbial community structures do
not correlate with the soil greenhouse gas production potential run counter to some studies that
have found a significant correlation between the structure and diversity of microbial
communities with microbial activities (Foulquier et al., 2013; S. He et al., 2015; Louis et al.,
2016). However the link between the structure of microbial communities and organic matter
decomposition is not conclusive (Schimel & Schaeffer, 2012), and these results suggest that
there is a degree of functional redundancy in the microbial community regarding carbon
production in created wetlands.
Invasive species control
The application of herbicide to control invasive species in the early fall of 2017 caused a
shift in the plant community from a near monoculture of Phalaris arundinacea (Reed Canary
Grass) in the spring and summer of 2017 to a near vegetation free wetland in the spring and
summer of 2018. Our examination of the microbial community in the wetland over two years
showed a significant shift in the microbial community both seasonally and between years,
potentially due to the effects of invasive species control measures used at the site. This supports
findings in previous studies that show significant differences in wetland microbial communities
in areas with invasive species (Angeloni et. al., 2006). Seasonal measurements showed that as
time went on the shifts in the microbial community appeared to become more pronounced. The
microbial community in the spring after the herbicide application resembled the microbial
community of the summer before the herbicide application. However, in measurements of the
microbial community in the summer after the herbicide application the microbial community
strongly diverged from any pre-herbicide measurements. This suggests that the changes in the
28
microbial community in response to vegetation die-off associated with herbicide application may
require time to fully develop. Alternatively, this shift could be due to other environmental
changes not quantified in this study and the lack of a herbicide control, due to the fact that
management decisions were outside of the control of this study, limits the ability to draw direct
conclusions about the impacts of invasive species control.
Our study also found that there was a significant decrease in microbial diversity in the
year following the use of herbicides to control invasive species, contrasting with studies of
agricultural soils that found no significant effect of herbicide application on microbial diversity
(Lupwayi et al., 2004). It is possible that the changes we observed are due to other environmental
changes not quantified in this study; however, changes in plant community composition have
been shown to alter microbial community structure in past studies (Angeloni et. al., 2006). Our
results are similar to previous studies that found long term shifts in microbial communities on
agricultural fields that have had herbicide applied over several years (Seghers et al., 2003) and
also align with findings of studies that found a shift in microbial communities over short time
periods of time, twenty and thirty days, due to a single herbicide application in agricultural
(Sebiomo et al., 2011) and forested ecosystems (Ratcliff et al., 2006).
Alternatively, the long-term shift in soil microbial communities observed in this study
may not be due to direct effects of the herbicide but to the downstream changes in the plant
community and soil nutrients following invasive species eradication efforts. Vegetated and
unvegetated areas of wetlands have been shown to have different microbial community
compositions (Arroyo et al., 2015), suggesting that our observed shift in microbial community
structure may be due in part to the removal of the plant community rather than direct effects of
the glyphosate herbicide. Replacing invasive species with native species also results in changes
29
to the soil microbial community (Kourtev et al., 2003), suggesting that the microbial community
could shift further as native plants begin to grow in the wetland. The application of herbicide was
also followed by significant increases in soil organic matter and soil phosphorus, likely due to
the addition of biomass to the system following plant die-off from herbicides as well as due to
the herbicide itself. Numerous studies have noted correlations between soil chemistry and
microbial community structure (Ahn & Peralta, 2009; Arroyo et al., 2015; Lee et al., 2019),
suggesting that the changes in soil chemistry as a result of invasive species control may be in
part responsible for some of the long term changes in the microbial community. The addition of
fresh plant litter in particular has been shown to drive shifts in microbial community composition
(Yan et al., 2018).
Herbicide is commonly used as a management tool to control invasive plant species in
wetlands, but the impacts of this and other invasive species control measures on the underlying
microbial communities is not well understood. The development of more advanced microbial
ecology and bioinformatics tools and techniques has opened the door to further study of the
impacts of herbicide on soil microbial communities (Jacobsen & Hjelmsø, 2014). Further study
is merited to disentangle the direct impact of herbicides, from vegetation change and plant litter
inputs associated with invasive species control, on microbial communities and ecosystem
functions in created wetlands.
Broader Impacts/Conclusions
Differences in land use history, management practices, and hydrologic conditions in
created wetlands can have impacts on the underlying soil chemistry and microbial communities
in wetlands. Differences in land use history across the three created wetlands appeared to
30
contribute to distinct soil chemistry regimes. Agricultural land use history was correlated with
increased soil nutrient levels in these wetlands. Elevated nutrient levels should be expected when
wetlands are created on agricultural lands and are consistent with global patterns (MacDonald et
al., 2012). Differences in soil nutrient levels were significantly correlated with differences in the
microbial communities, however hydrology and soil water content appeared to be the strongest
driver of microbial community composition. Despite the observed differences in microbial
community structure there were no differences in anaerobic CO2 or CH4 production potential in
these wetlands, indicating that there is redundancy in wetland microbial communities relating to
carbon metabolism. This redundancy suggests that changes in microbial communities leading to
a change in greenhouse gas (GHG) production potential may not be an important consideration
in the design and management of created wetlands. There were also no apparent differences of
GHG production potential in relation to differences in DOM structure between the wetlands
suggesting that this should not be a significant consideration when trying to mitigate GHG
emissions during wetland construction.
This study also found that invasive species control using herbicides to eradicate
vegetation may lead to changes in soil chemistry and microbial community composition of a
created wetland. In the year following invasive species control, there was a substantial shift in
the plant community as well as an increase in soil organic matter and soil phosphorus. These
changes likely contributed to the observed changes in the microbial community. These results
show that the long-term impacts on microbial communities and function should be considered
when making decisions about how to control invasive species in wetlands.
31
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