Dioxin Exposure Impairs BMP-2-Mediated Spinal Fusion in a Rat Arthrodesis Model by Erin L. Hsu,...

Dioxin Exposure Impairs BMP-2-Mediated Spinal Fusion in a Rat Arthrodesis Model

by Erin L. Hsu, Kevin Sonn, Abhishek Kannan, Sharath Bellary, Chawon Yun, Sohaib Hashmi, John Nelson, Marco Mendoza, Michael Nickoli, Jason Ghodasra, Christian Park, Sean Mitchell, Amruta Ashtekar, Anjan Ghosh, Akshay Jain, Stuart R. Stock,

and Wellington K. Hsu

J Bone Joint Surg AmVolume 97(12):1003-1010

June 17, 2015

©2015 by The Journal of Bone and Joint Surgery, Inc.

Relative abundance of Cyp1a1 (Fig. 1-A), Cyp1a2 (Fig. 1-B), and Cyp1b1 (Fig. 1-C) mRNAs in rat livers as determined by quantitative polymerase chain reaction.

Erin L. Hsu et al. J Bone Joint Surg Am 2015;97:1003-1010

©2015 by The Journal of Bone and Joint Surgery, Inc.

Representative radiographs depicting bridging bone formation or lack thereof between the L4 and L5 transverse processes of vehicle-treated controls and dioxin-treated and dioxin-recovery

animals.

Erin L. Hsu et al. J Bone Joint Surg Am 2015;97:1003-1010

©2015 by The Journal of Bone and Joint Surgery, Inc.

Mean fusion scores (Fig. 3-A) and rates (Fig. 3-B) as determined by manual palpation by three independent, blinded observers using a previously established scoring system, with 0 indicating

no bridging bone; 1, unilateral bridging; and 2, bilateral bridging...

Erin L. Hsu et al. J Bone Joint Surg Am 2015;97:1003-1010

©2015 by The Journal of Bone and Joint Surgery, Inc.

MicroCT-based quantification of new bone formation in fused spines from vehicle-treated controls and dioxin-treated and dioxin-recovery animals.

Erin L. Hsu et al. J Bone Joint Surg Am 2015;97:1003-1010

©2015 by The Journal of Bone and Joint Surgery, Inc.

Three-dimensional renderings of representative fused and unfused spines from each group.

Erin L. Hsu et al. J Bone Joint Surg Am 2015;97:1003-1010

©2015 by The Journal of Bone and Joint Surgery, Inc.

Figs. 5-A through 5-E Histologic sections of representative specimens from control, dioxin, and dioxin-recovery groups (magnification, ×2).

Erin L. Hsu et al. J Bone Joint Surg Am 2015;97:1003-1010

©2015 by The Journal of Bone and Joint Surgery, Inc.