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DBT-NER-PROJECT
ADVANCED ANIMAL DISEASE DIAGNOSIS AND MANAGEMENT CONSORTIUM
Roadmap, 2014-16
After a threadbare discussion and interaction among the members, a consensus
was reached to prepare a road map with common minimum programme so that a visible
progress can be achieved. Identified areas are as follows:
1. Prioritization of livestock species to be covered in the first phase: Based on the
priority of the North Eastern Region, diagnostic facilities will be created in core labs with
the help of national laboratories for diseases of pigs, goats and ducks. Intensive
investigation on diseases affecting these species will be carried out in NE states during
the first two years. However, diagnostic activities on other animals will also continue at
the same time.
2. Infectious agents to be studied : Infectious agents affecting pigs, goats and ducks are
grouped as endemic and emerging/re-emerging.
Animal
species
Infectious
agent
Endemic Emerging/Re-
emerging
Pigs
Bacteria Salmonella, Pasteurella, Erysipelas,
Carbapenem resistant E Coli. Anthrax;
Clostridium, Brucella
Oedema disease,
Carbapenem
resistant E Coli.
Virus CSFV, PPV, RV, JEV, Rota, PRRS Circo virus, PEDV,
Swine Influenza,
Nipah virus, TGEV,
Swine Pox, TTV
Parasites Nematodes ( Ascaris, oesophagostomum,
stephanurus, gnathostoma, capillaria etc),
trematodes ( Fasciola, opisthorchis,
artyfechinostomum, gastrodiscoides), Larval
cestode ( Cysticercus cellulosae, hydatids) ,
Haemoprotozoa, coccidia
Goats
Bacteria Pasteurella, Clostridia, Listeria,
Mycobacterium paratuberculosis,
Mycoplasma, Anthrax, Brucella
Virus PPR, Orf, BTV,Goat pox, Para influenza, Nipah,Encephalities
causing virus
Parasites Trematodes (Fasciola, Amphistome,
Schistosomes), GI Nematodes (Haemonchus,
mecistocirrus, oesophagostomum,
bunostomum, cooperia), Cestodes (Moniezia,
stilesia, avitellina, Taenia & Echinococcus),
coccidian and haemoparasites (Babesia,
theileria, anaplasma).
Ducks
Bacteria Pasteurella, Salmonella, Botulism,
Mycotoxin
Virus DPV DV Hepatities, AI
Parasites Trematodes (Echinostoma, tracheophilus,
prosthogonimus), Nematodes ( Ascaridia,
heterakis, porocaecum, tetramers), Cestode
(Raillietina, Fibbriaria, hymenolepis),
coccidi and haemoparasite.
Each national partner and NE core lab will take responsibility to develop
diagnostics and confirm diseases prevalent in eight states. Action to be taken by the
respective centre as assigned below:
Action to be taken by NIVEDI :
Animal species Endemic & emerging/re-emerging infectious agents
Pigs
Bacteria Brucella, AMR
Virus CSFV, PRRS,TTV
Goats Bacteria Brucella, AMR, Mycoplasma
Action to be taken by NIHSAD:
Animal species Endemic & emerging/re-emerging infectious agents
Pigs Virus Nipah, Ebola, Influenza A virus, PRRS
Goats *Virus CCHF, NSD, RVF
Ducks Virus Influenza A virus
Action to be taken by NRCE/VTCC :
Animal species Endemic & emerging/re-emerging infectious agents
Pigs
Bacteria Salmonella, Pasteurella, Erysipelas
Virus RV, JEV, Swine Influenza,
Parasites Trypanosoma (rest of the component to be
taken up at Khanapara and NRCE will assist
for other parasites)
Goats
Virus Para influenza, Encephalities causing virus
Parasites Trypanosome (rest of the component to be
taken up at Khanapara and NRCE will assist
for other parasites)
Action to be taken by NRC on pigs:
Animal species Endemic & emerging/re-emerging infectious agents
Bacteria Salmonella, Pasteurella, Brucella
Pigs Virus CSFV, RV, Circo, Parvo virus
Action to be taken by Core Lab I :
Animal
species
Endemic & emerging/re-emerging infectious agents
Pigs
Bacteria Salmonella, Pasteurella, Brucella, Erysipelas, AMR, Anthrax;
clostridium ,Oedema disease
Virus CSFV, PPV, RV,PRRS, Swine Pox.
Parasites
, vector
Nematodes ( Ascaris, oesophagostomum, stephanurus,
gnathostoma,capillaria etc), trematodes ( Fasciola, opisthorchis,
artyfechinostomum, gastrodiscoides), Larval cestode ( Cysticercus
cellulosae, hydatids) , Haemoprotozoa, coccidian, vector
Goats Bacteria Brucella, AMR, Pasteurella, Clostridia, Listeria,
Mycobacterium paratuberculosis, Anthrax
Virus Orf
Parasites
, vector
Trematodes (Fasciola, Amphistome, Schistosomes ), GI Nematodes
(Haemonchus, mecistocirrus, oesophagostomum, bunostomum,
cooperia), Cestodes (Moniezia, stilesia, avitellina, Taenia &
Echinococcus), coccidian and haemoparasites (Babesia, theileria,
anaplasma). vector
Ducks
Bacteria Pasteurella, Salmonella, Botulism, Mycotoxin.
Virus DPV
Parasites Trematodes (Echinostoma, tracheophilus, prosthogonimus),
Nematodes ( Ascaridia, heterakis, porocaecum, tetramers), Cestode
(Raillietina, Fibbriaria, hymenolepis), coccidi and haemoparasite.
Action to be taken by Core Lab II :
Animal
species
Endemic & emerging/re-emerging infectious agents
Pigs
Bacteria Salmonella, Erysipelas, Brucella, AMR,
Virus CSFV, JEV,
Circo virus, PRRS
Parasites,
vector
Nematodes ( Ascaris, oesophagostomum, stephanurus,
gnathostoma,capillaria etc), trematodes ( Fasciola, opisthorchis,
artyfechinostomum, gastrodiscoides), Larval cestode ( Cysticercus
cellulosae, hydatids) , Haemoprotozoa, coccidian, vector
Goats Bacteria Brucella, AMR, Listeria
Virus Nipah
Parasites,
vector
Trematodes (Fasciola, Amphistome, Schistosomes ), GI Nematodes
(Haemonchus, mecistocirrus, oesophagostomum, bunostomum,
cooperia), Cestodes (Moniezia, stilesia, avitellina, Taenia &
Echinococcus), coccidian and haemoparasites (Babesia, theileria,
anaplasma), vector
Bacteria Pasteurella, Salmonella, Afla toxin
Ducks
Virus DPV
Parasites Trematodes (Echinostoma, tracheophilus, prosthogonimus),
Nematodes ( Ascaridia, heterakis, porocaecum, tetramers), Cestode
(Raillietina, Fibbriaria, hymenolepis), coccidia and haemoparasite.
Action to be taken by Core Lab III :
Animal species Endemic & emerging/re-emerging infectious agents
Pigs
Bacteria Salmonella, Pasteurella, Brucella, AMR, E Coli.,
Oedema disease
Virus PRRS
Parasites,
vector
Nematodes ( Ascaris, oesophagostomum, stephanurus,
gnathostoma,capillaria etc), trematodes ( Fasciola,
opisthorchis, artyfechinostomum, gastrodiscoides), Larval
cestode ( Cysticercus cellulosae, hydatids) ,
Haemoprotozoa, coccidian, vector
Goats Bacteria Brucella, AMR, Pasteurella, Listeria, Mycobacterium
paratuberculosis
Parasites,
Vector
Trematodes (Fasciola, Amphistome, Schistosomes ), GI
Nematodes (Haemonchus, mecistocirrus, oesophagostomum,
bunostomum, cooperia), Cestodes (Moniezia, stilesia,
avitellina, Taenia & Echinococcus), coccidian and
haemoparasites (Babesia, theileria, anaplasma), vector
Ducks
Bacteria Pasteurella, Salmonella, Afla toxin
Parasites Trematodes (Echinostoma, tracheophilus, prosthogonimus),
Nematodes ( Ascaridia, heterakis, porocaecum, tetramers),
Cestode (Raillietina, Fibbriaria, hymenolepis), coccidi and
haemoparasite.
*Goat diseases on PPR, BTV, Goat pox will be covered from 3rd
year onwards as there
are DBT funded ongoing projects on these agents.
3. Strengthening of Human Resource : The prime objective of the project is to develop
disease diagnostic competency in NER Labs through training human resources.
Accordingly certain topics are identified and decided to carry out training programmes at
national as well as at core lab levels within second year of the project as per tentative
time schedule indicated below:
Training
Module
Training Details Tentative
date
Action to be
taken by
Trainees
Passive
survey
Survey strategy, data entry, record
keeping
22-24
April, 2015
NE core Lab-
I/NIVEDI
Core lab
personnels,
Training carried out at National labs, necessary TA/DA for SAHD officers should be
arranged by the host institutes and at core labs fund should be arranged by core lab.
However, matter should be taken up at DBT level to obtain permission for payment of
TA & DA from Training & also allotment of more fund under head Training.
4. Development of Laboratory capability at Core Labs :
a. Protocols standardized/developed as per OIE guidelines and diagnostic kits/reagents/
positive control/negative control available at national labs as well as in other core labs
should be shared with NE core labs. Feed back of test results can be used as validation of
the tests. Such protocols can be finalised as common SOPs for use in the diagnosis of
Active
survey
Sampling strategy, collection of
samples, preservation &
dispatching
state Animal
Husbandry
personnels
TADs
samplings
Lab, sample handing, advanced
diagnosis of TADs
April, 2015
NIHSAD Core lab
personnels,
SAHD
personnels
Field
diagnosis
Basic Microbial, pathological,
parasitic methods – Gross
diagnosis, Microscopical,
Serodiagosis,
June, 2015
NE core Labs
I/II/III
state Animal
Husbandry
personnels
Advanced
diagnosis
Molecular diagnostic protocols
(OIE/Inhouse), Serodiagnosis,
isolation techniques
July, 2015
NIVEDI/
NIHSAD/NRC
E/VTCC/NRC
pigs
NE core
Labs/NIVEDI/
HSADL/NRC
E/VTCC/NRC
pigs
Disease
mapping
GIS application, analysis and
interpretation
May, 2015
NIVEDI/
NE core Labs/
NIVEDI
Core lab
personals,
state Animal
Husbandry
personals
Software
management
& Data
analysis
Data analysis, data interpretation
& presentation
Reposition Methods on reposition & storage,
Sample coding, record keeping,
preservation, dispatch.
August,
2015
NRCE/ VTCC
diseases in NE states. Necessary MTA should be completed by respective National
partners so as to dispatch kits/reagents to respective centres within 2 months.
Agents Type of SOPs/kits
available
Action to be taken by SOPs/kits provided to
PRRSV
PCR protocol NIHSAD, Core lab II,
NIVEDI
Core lab I & III
Indirect ELISA kits NISAD Core labs I,II,III
JEV PCR protocol NRCE, Core lab II Core lab I & III
HI protocol/kits NRCE Core labs I,II,III
AIV Ag –Ab kits NIHSAD Core labs I,II,III, Field
labs
NDV PCR protocol Core lab I Core labs II,III,
Field labs HI
protocol/reagents
DPV PCR protocol Core lab I Core lab II, III
i-ELISA protocol
RV PAGE, PCR
protocol
Core lab I, NRCE Core labs II,III
Isolation protocol Core lab I
Swine
pox
PCR protocol Core lab I Core labs II,III
Fowl pox PCR protocol Core lab I Core labs II,III
Swine
influenza
PCR protocol NRCE Core labs I,II,III
PPR,
BTV,
Goat Pox
PCR protocol NIVEDI Core labs I,II,III
AMR PCR protocol NIVEDI, Core lab III Core labs I,II
Brucella PCR protocol NIVEDI Core labs I,II,III,
Field labs Ab kits
Clostridia PCR protocol Core lab I Core labsII,III,
National lab Isolation protocol
Listeria PCR protocol Core lab II Core labs I, III
Isolation protocol
Trypenos
oma
PCR protocol NRCE Core labs I,II,III, Field
labs
ELISA protocol
Babesia,
Theileria
PCR protocol Core lab I Core labs II,III, National
lab
b. Common protocols used by Core lab I, II and NIVEDI for detection of CSFV by PCR
and Ag-Ab detection tests and isolation of the virus will be validated by ring tests.
Consensus protocols in the light of EU SOPs will be finalized and developed as common
SOP for use in diagnosis of CSFV in NE states. Action will be taken by Core lab I and
NIVEDI.
c. For development of SOPs to detect PPV, Circo virus, PEDV, TGEV, Nipah virus, TTV,
Duck hepatitis virus, Erysipelas, Oedema disease, cysticarcus, hydatid, coenurus, qPCR
for Theileria, Babesia, , Aflatoxin, Anthrax should be initiated by identified centres and
necessary clinical/tissue samples should be provided from field by core labs for
standardization of tests.
5. Development of Laboratory Capability at Field units :
a. A diagnostic laboratory manual will be prepared covering necessary formats,
protocols for preparation of various reagents, diagnosis of microbial, parasitic,
toxicological diseases and clinic-pathological, pathological investigations etc. Action
to be taken by scientists from NIVEDI, VTCC, Core labs I, II and III (2 months).
b. All 3 core labs should arrange training for field staffs for collection of disease data,
samples and despatch of samples to the core labs. Necessary reagents, plastic and
glass wares, preservation/transport media should be provided. To begin with
diagnostic activities quick and handy diagnostic kits/reagents should be arranged.
6. Formats to be prepared :
Type of formats Action to be taken (One month)
Passive survey format, outbreak survey, format
for Sero- survey, routine parasitic survey, check
list for collection of sample.
VTCC ; NIVEDI; core labs
Uniform sample code for tissue, sera, isolates
will be developed.
VTCC ; NIVEDI; core labs
Guidelines & formats for reporting of
investigation results for
bacteria/Virus/serologcal/
parasitic/toxic/pathological investigations.
NRCE
Sample entry & sample registry formats NIVEDI;VTCC ; core labs
Disease data base/ Accessibility to NADRES. NIVEDI
Development of Website Core labs I, II and NIVEDI
Monthly report format from field unit NIVEDI
7. Sampling Strategy :
a. Initially for 6 months survey will be confined in and around field diagnostic units of
the respective state as there are no funds available in the 1st year for SAHDs.
Information on number of villages, type of species available, No of animal
herd/unit/bird unit, Av animals per herd/unit, Av. birds per unit will be collected and
intimated to NIVEDI through core Labs. Action to be taken by PIs of SAHD and
Core lab I (31st March 2015).
b. NIVEDI will identify the village and framed the sample number, frequency for sera
samples, clinical samples, faecal samples in routine survey as well as during
outbreak. Action to be taken by NIVEDI (April 2015).
c. Samples will be collected as stated below jointly by project staffs and Field lab
incharges. All consumables/ medicines/vaccines to be used in field dung collection of
samples should be arranged by core labs.
Animal/bird Type of sample No. of sample/state
Pig
Sera sample (3month-1 yr age group) 200 (50 /F. Lab)
Blood smears (Haemoprotozoa) 100 (25 / F. Lab)
Vector survey
Nasal swabs 100 (25 / F. Lab)
Faecal samples (herd/unit basis of young,
grower, adult)
400 (100 / F. Lab)
Organs in dead carcass As per the mortality/
outbreak
Goat
Sera sample (3month-1 yr age group) 400 (100 /F. Lab)
Blood smears (Haemoprotozoa) 100 (25 / F. Lab)
Vector survey
Nasal swabs 200 (50 / F. Lab)
Faecal samples (herd/unit basis of young,
grower, adult)
400 (100 / F. Lab)
Organs in dead carcass As per the mortality/
outbreak
Duck
Sera sample (3month-1 yr age group) 100 (25 /F. Lab)
Blood smears (Haemoprotozoa) 40 (10 / F. Lab)
Tracheal swabs 40 (10 / F. Lab)
Faecal droopings (unit basis of young,
grower, adult)
200 (50 / F. Lab)
Organs in dead carcass Dead/ ailing bird
d. The participation of NERDDL is crucial and agreement regarding sample sharing,
diagnostic protocol implementation and disease informatics need to be worked upon.
e. Samples collected in field will be dispatched to core labs through project staffs,
messenger from field, through courier service /overnight service. Expenditure will be
borne by respective core lab. Between core labs samples will be transported by
project staffs/courier service at every month. Samples from core labs to national labs
will be dispatched at every 3/6months by courier/messenger. Emergency samples will
be sent by messenger. All cost involve in dispatching samples to national labs should
be arranged by respective national lab. Official permission for interstate transport of
samples / dispatch to national lab will be arranged by PIs of SAHD and National labs.
f. A nodal liaison representative at the National Institute level needs to be finalised and
communicated to all implementing partners.
g. There should be communication of fortnightly reports regarding work progress under
this project to Core lab I in order to implement a real time monitoring and evaluate
progress achieved by the implementing partners.
1
ROAD MAP OF THE PROJECT FOR THE YEAR 2016-19 AND
ACTION TO BE TAKEN :
1. Prioritization of diseases:Based on the infrastructure available for disease diagnosis
in the North Eastern Regionthe diseases need to prioritized using retrospective
disease data of last 10 year for species –wise prevalent in the region.
2. Development of diagnostics:Based on met analysis, diagnostics need be
developedfor the diseases prevalent in the region for respective species with the
help of national partners.
3. Surveillance of Emerging/Re-emerging diseases:-Be undertaken in a holistic way
using proper sampling & diseases of regional importance.
4. Research/Actions to be undertaken by each institute:
Each national partner and NE core lab will take responsibility to develop diagnostics
and confirm diseases prevalent in eight states. Action to be taken by the respective
centre as assigned below:
a. NIVEDI, Bangaluru :
Molecular epidemiology & phylogenic analysis of various diseases including
risk & met analysis.
Development of fore-warning models with impact analysis of identified
diseases of national importance.
b. NIHSAD, Bhopal:
Human resource development & capacity building of officials of NER to
handle emerging /exotic diseases in the region..
Molecular genomics& diagnosis of trans-boundary/exotic diseases including
phylogenic analysis.
c. NRCE/VTCC, Hisar :
Molecular genomics& diagnosis of equine diseases except influenza virus
including phylogenic analysis & risk assessment.
Proper maintenance of isolates received from other units with cataloguing.
d. NRC on pigs, Guwahati
Development of infrastructure for proper conventional & molecular diagnosis
of pig bacterial diseases.
2
e. Core Lab I, II & III :
Development of infrastructure for proper conventional & molecular diagnosis
including molecular epidemiological studies on different livestock & poultry
diseases in the region with their prioritization
Development of competency & core competence in handling different
diseases of regional importance including emerging & exotic diseases,
Fore-warning disease modelling, risk assessment & impact analysis in
cooperation with NIVEDI,
5. Diagnostics or Vaccine development:-
It should be limited to one or two labs & in case any institution is developing
any diagnostic/vaccine need be validated in other labs & that institution
would be responsible for its commercialization.
SOP’s already developed need be standardized as per OIE/WHO norms &
guidelines.
Any new SOP developed by any institution need be validated & standardized
& Core Labs be involved in such procedures.
6. Strengthening of Human Resource:
Developdisease diagnostic competency in NER Labs through training human
resources in following training programmes.
Name of Training Name of Institution
imparting training
Name of Labs receingtrainig
Lab, sample handing, advanced diagnosis of TADs
NIHSAD Core lab &, SAHD personnel
Molecular diagnostic protocols (OIE/Inhouse), Serodiagnosis, isolation techniques
NIVEDI/ NIHSAD/NRCE/VTCC/NRC pigs
NE core Labs/NIVEDI/HSADL/NRCE/VTCC/NRC pigs
GIS application, analysis and interpretation
NIVEDI/ NE core Labs/ NIVEDI
Core lab personals, state Animal Husbandry personals
Data analysis, data interpretation & presentation
Methods on reposition & storage, Sample coding, record keeping, preservation, dispatch.
NRCE/ VTCC
3
6. Development of Laboratory Capability at Field units :
a. Point of care diagnostic developed by National Labs, Core Labs should made
available at field labs. Besides other conventional diagnostics tools and reagents
should be procured by respective core labs and distributed to field labs.
b. A diagnostic laboratory manual/protocols be made available on website.
c. Training for field staffs will continue.
d. Scientific staffs from respective core labs/ national labs visit field labs in
convenient intervals and perform diagnosis in the field labs along with field staff.
7. Formats to be prepared :
Type of formats Action to be taken
Passive survey format, outbreak survey, format for Sero-
survey, routine parasitic survey, check list for collection of
sample.
VTCC ; NIVEDI; core labs
Uniform sample code for tissue, sera, isolates will be
developed.
VTCC ; NIVEDI; core labs
Guidelines&formats forreporting of investigation results for
bacteria/Virus/serologcal/parasitic/toxic/pathological
investigations.
NRCE
Sample entry &sample registry formats NIVEDI;VTCC ; core labs
Disease data base/ Accessibility to NADRES. NIVEDI
Development of Website Core labs I, II and
NIVEDI
Monthly report format from field unit NIVEDI
These formats should be evaluated through an expert committee &cross
validations need be done to maintain uniformity and updating the same.
8. Sampling Strategy & Disease Risk Analysis:
a. Risk analysis and KAP formats should be prepared by NIVEDI in association with
Core Labs
b. Field study should be carried out as routine disease survey and outbreaks
investigation.
c. Villages surrounding to the Field labs should be identified . NIVEDI will identify
the village and framed the sample number, frequency for sera samples, clinical
samples, faecal samples in routine survey as well as during outbreak. Along with
routine survey data on Risk analysis and KAP will be collected.
4
d. Awareness & animal health camps should be organized in identified strategic
locations to understand farmers problems and collect samples. Awareness
through different media & distribution of leaflets in vernacular/Hindi language.
e. Samples will be collected as stated below jointly by project staffs and Field lab
incharges. All consumables/ medicines/vaccines to be used in field dung
collection of samples should be arranged by core labs.
9. Sharing of Samples & Data:Needs to be continued.
a. To compile disease map linkage should be established with ongoing disease
related projects under DBT, ICAR, DSTin NER to share samples/data.
b. The participation of NERDDL is crucial and agreement regarding sample sharing,
diagnostic protocol implementation and disease informatics need to be worked
upon.
c. Samples collected in field will be dispatched to core labs through project staffs,
messenger from field, through courier service /overnight service. Expenditure
will be borne by respective core lab. Between core labs samples will be
transported by project staffs/courier service at every month. Samples from core
labs to national labs will be dispatched at every 3/6months by
courier/messenger. Emergency samples will be sent by messenger. All cost
involve in dispatching samples to national labs should be arranged by respective
national lab. Official permission for interstate transport of samples / dispatch to
national lab will be arranged by PIs of SAHD and National labs.
10. Strengthening of National Repository.It should be continued & national repository
at VTCC need be strengthened by sharing the samples & it would not be proper to
develop another facility of same kinds due to involvement of huge manpower &
financial implications.
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