cubense Tropical Race 4 Varietal screening trial to identify banana varieties highly tolerant or...

www.nt.gov.au

Varietal screening trial to identify

banana varieties highly tolerant or

resistant to Fusarium oxysporum f.sp

cubense Tropical Race 4

Sharl Mintoff

DEPARTMENT OF PRIMARY INDUSTRY AND RESOURCES

www.nt.gov.au

Varietal trail screening for TR4 resistance

• Banana Plant Protection Program

– BA10020

• Assessing 27 Banana varieties

for resistance to TR4

• Trial taking place at Coastal

Plains Research Farm (NT)

• Planting occurred in June 2016

www.nt.gov.au

Varietal trail screening for TR4 resistance

• Currently 15 months into trial and

26 of 27 varieties now in first ratoon

• Trial includes 4 reference varieties

– Williams (Susceptible)

– GCTCV 218 (Tolerant)

– FHIA 01 (Resistant)

– FHIA 25 (Highly resistant)

• This talk mainly focuses on the

health of the mother plants

Variety GCTCV 105/6

Cav

end

ish

Williams GCTCV 215

DPM25 GCTCV 217

Dwarf Nathan GCTCV 218 (Formosana)

Dwarf Parfitt Offtype CJ19

SH-3362 Auto-tetraploid SH-3436

Pare

nts

/ H

ybri

ds

SH-3142 SH-3641

SH-3217 SH-3656

SH-3362 SH-3748

FHIA-01 (Goldfinger) FHIA-18

FHIAFHIA-02 FHIA-25

FHIA-03 FHIA-26

Senorita Pisang Gajih Merah

Oth

er

Dwarf Ducasse M. acuminata ssp. banksii

www.nt.gov.au

Varietal trail screening for TR4 resistance

• Currently 15 months into trial and

26 of 27 varieties now in first ratoon

• Trial includes 4 reference varieties

– Williams (Susceptible)

– GCTCV 218 (Tolerant)

– FHIA 01 (Resistant)

– FHIA 25 (Highly resistant)

• This talk mainly focuses on the

health of the mother plants

Variety GCTCV 105/6

Cav

end

ish

Williams GCTCV 215

DPM25 GCTCV 217

Dwarf Nathan GCTCV 218 (Formosana)

Dwarf Parfitt Offtype CJ19

SH-3362 Auto-tetraploid SH-3436

Pare

nts

/ H

ybri

ds

SH-3142 SH-3641

SH-3217 SH-3656

SH-3362 SH-3748

FHIA-01 (Goldfinger) FHIA-18

FHIAFHIA-02 FHIA-25

FHIA-03 FHIA-26

Senorita Pisang Gajih Merah

Oth

er

Dwarf Ducasse M. acuminata ssp. banksii

www.nt.gov.au

Symptoms of Foc

• External symptoms

– Splitting of the pseudostem

– Yellowing and eventual wilting

of older leaves

– Death of leaves

– Death of plant

• Internal symptoms

– Discoloration of xylem

tissue

www.nt.gov.au

Criteria assessed

Disease assessments

• Assessments done monthly,

once infections became

promininantly fortnightly

• Presence of external

symptoms

• Death dates of plant

• Presence of internal

symptoms upon death

Agronomic assessments

• Date of bunch emergence

• Plant height at bunch

emergence

• Date of harvest

• Bunch weights

• Presence of internal

symptoms

www.nt.gov.au

Susceptible varieties

• Extensive disease symptoms

• Williams

- Death before harvest

• DPM25

- Death before

bunching/harvest

• Dwarf Parfitt offtype

– Death before bunchingDPM 25 D. Parfitt

OfftypeWilliams

www.nt.gov.au

Susceptible varieties (continued)

FHIA 26 SH-3656Senorita

• Senorita

- Death before bunching

• FHIA 26

- Death before bunching

• SH-3656

– Death before harvest

• Few made it harvest

www.nt.gov.au

• Majority of plants make it to

harvest

• No internal or external symptoms

observed

Resistant varieties

– FHIA 01

– FHIA 02

– FHIA 03

– FHIA 18

– FHIA 25

– Pisang Gajih

Merah

– SH-3362

– SH-3217

– SH-3142

– SH-3748

– GCTCV 215

– CJ19

FHIA 01 FHIA 25PG Merah

CJ19 GCTCV 215SH-3217

www.nt.gov.au

• SH-3362 Auto-tetraploid

– No signs of bunching, cut down 12

months after planting

– No symptom observed

• M. acuminata ssp. banksii

– North QLD Native

– Approx. 40% died in the first 6

months (unlikely due to TR4)

– Remaining plants severely

stressed

Problematic varieties

www.nt.gov.au

Susceptibility to Foc TR4

0

20

40

60

80

100

% inte

rnal sym

pto

ms

Variety

0

20

40

60

80

100

Will

iam

s

DP

M25

Dw

arf

Nath

an

Dw

arf

Parf

itt…

GC

TC

V 1

05

/6

GC

TC

V 2

15

GC

TC

V 2

17

GC

TC

V 2

18

…

CJ19

FH

IA-0

1…

FH

IA-0

2

FH

IA-0

3

FH

IA-1

8

FH

IA-2

5

FH

IA-2

6

SH

-343

6

SH

-364

1

SH

-365

6

SH

-374

8

SH

-336

2 A

uto

-…

SH

-314

2

SH

-321

7

SH

-336

2

Sen

orita

Dw

arf

Du

casse

Pis

ang

Ga

jih…

M.

acum

ina

ta…

Pe

rce

nt d

ea

th

Variety

Death (TR4) Death (other)

www.nt.gov.au

0

10

20

30

40

50

60

70

80

90

100

Pe

rce

nt

pla

nt

su

rviv

al

to h

arv

es

t

Variety

Harvested Remaining

Survival of plants

www.nt.gov.au

0

10

20

30

40

50

60

70

80

90

100

Pe

rce

nt

of

sym

pto

m p

res

en

ce

/ a

bs

en

ce

Variety

Present Absent

Survival of plants (internal symptoms at harvest)

www.nt.gov.au

Weeks to bunch emergence

0

10

20

30

40

50

60W

eeks p

ost

pla

nti

ng

Variety

www.nt.gov.au

Plant height at bunch emergence

0

50

100

150

200

250

300

350

400H

eig

ht (c

m)

Variety

www.nt.gov.au

Weeks to harvest

0

10

20

30

40

50

60W

eeks p

ost pla

nting

Variety

www.nt.gov.au

Bunch weight

0

5

10

15

20

25

30

35

40

Weig

ht (K

g)

Variety

www.nt.gov.au

Conclusions

Assessments of 1st ratoon plants is ongoing

– Determine if resistance seen in Mother plants also apparent in ratoon

plants

Several varieties have shown promising resistance to TR4

– Agronomic characteristics may not be ideal in their current state

– Resistance vs. acceptable market quality (need both)

– Promising candidates have been selected for Mutagenesis in attempt

agronomic qualities of resistant plants in BA14014

www.nt.gov.au

Acknowledgements

Vu Tuan Nguyen (DPIR)

Chris Kelly (DPIR)

Lucy Tran-Nguyen (DPIR)

Sam Cullen (DPIR)

Cassie McMaster

Gavin Hunter

Sharon Hamill (DAF)

Stewart Lindsay (DAF)

Jeff Daniells (DAF)

Tony Pattison(DAF)

www.nt.gov.au

Thank you