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2013L-3000 Series High Performance Liquid Chromatographic
RIGOL TECHNOLOGIES,INC.
Chemical Analysis Catolog
Company Profile
1
Founded in 1998, RIGOL Technologies, Inc. is an ISO9001:2000
Quality Management System and ISO14001:2004 Environmental
Management System Certified company, an emerging T&M
leader and a technology innovator in Chemical Analysis and
Electronic Measurement. RIGOL's premium line of products
includes HPLC and UV-Vis Spectrophotometers, and General
Purpose, RF Communication Electronic Instruments, which help
the engineers, researchers, educators to address their
measurement challenges in confidence with better affordability
than ever before.
RIGOL is headquartered in Beijing, China, with the branch offices
in Cleveland, OH, United States and Munich, Germany. The
company's 400 employees and more than 150 distributors or
representatives offer products and services in over 60
countries/regions on six continents. More information about
RIGOL is available at www.rigol.com.
2
L-3000 Series HPLC utilizes technologies that take liquid chromatographic analysis beyond the
conventional limitation and achieve the level of performance that today’s applications demand.
L-3000 Series High Performance Liquid Chromatography
BCEIA Gold Prize
Up to 8000PSI operational pressure. Higer working pressure extends the dynamic range in the application of chromatographic analysis, and improves the durability and reliability of the entire system.
Up to 2.5 AU linear range of the detector, allows accurate analysis for widely varying sample concentrations, either with high concentrated substance(s) or trace component(s).
Up to 100Hz data sampling rate, allows sharper peak(s) detection and enables ultra fast analysis.
Proprietary automatic solvent-compressibility compensation and pulsation dampening technologies enable excellent analysis reproducibility.
As low as 5x10-9 g/mL detection limit, creating higher sensitivity with lower noise floor.
Proprietary pump design and precision fabrication process result in excellent system durability.
User-friendly HPLC workstation, complies with the FDA 21 CFR Part 11, GLP, GMP regulations.
LOWERDetection Limit
HIGHERReproducibility
BETTER
Durability
MORE
user-friendly
3
L-3200 Delivery Pump
◎ Maximum operational pressure up to 8000PSI◎ Higher gradient accuracy (±1%) and reproducibility (≤0.15% RSD)◎ Proprietary automatic solvent-compressibility compensation and pulsation dampening technologies◎ Proprietary multi-flow mixer, dramatically reduces delay-volume, improving response to gradient changes
A variety of pump types, isocratic/binary/quaternary, ideal for widely varying applications
Isocratic pump for regular QA/QC usage Binary pump for high-throughput & fast analysis
Quarternary pump for pharmaceutical, food and environmental test.
isocratic
inlet check valve
outlet check valve
to sampler and chromatographic column
to waste liquid
mixer & damper
purge valve
binary gradient
inlet check valve
inlet check valve
outlet check valve
outlet check valve
to sampler and chromatographic column
to waste liquid
mixer & damper
purge valve
outlet check valve
to sampler and chromatographic column
to waste liquid
quarternary
vacuum degassing chamber
quarternary gradient valve
inlet check valve
mixer & damper
purge valve
4
10PSI
0.12% 0.14%0.16%
0.15%
0.17%0.18%
0.17%0.13%
0.14%
0.15%
L-3200 Delivery Pump
A higher pressure limit allows a wider flexible range of chromatographic separation conditions and higher efficiency.
Proprietary pulsation dampening technology keeps the pressure pulsation less than 10PSI, creating improved qualitative repeatability.
Precision automatic solvent-compressibility compensation and pulsation dampening, bring excellent gradient accuracy and repeatability.
Lower delay volume enables a cleaner & faster response to the gradient changes.
Other HPLC system
Faster analysisHigher resolutionBetter efficiency
Wider flow range
8000PSI
6000
4000
5 mL/min 10 mL/min
0
120
0
150
mAU
100
50
Time [min]
200 40 60 80 100
·Column: back pressure tube (L 1m, I.D. 0.127mm)·Mobile phase A: water·Mobile phase B: 0.1% isopropanol aqueous solution·Flow rate: 1.0 mL/min·Back pressure: 1000PSI·Detection wavelength: 265nm·Temperature: 25℃·Mobile phase B: 0-100% with 10% step-increment·Continuous runs: 3 times
1
0
150
mAU
100
50
Time [min]
65432
·Column: back pressure tube (L 1m, I.D. 0.127mm)·Mobile phase A: water·Mobile phase B: 0.1% isopropanol aqueous solution·Flow rate: 1.0 mL/min·Back pressure: 1000PSI·Detection wavelength: 265nm·Temperature: 25℃
Binary pump Without damper 400μL
Binary pump With standard damper 1100μL
Quarternary pump With standard damper 1500μL
PW=2.598s
100Hz
25Hz
5Hz
1Hz
0.5Hz
PW=2.268s
PW=3.004s
PW=3.886s
PW=4.002s
5
100Hz data sampling rate means that detection of peaks sharper than 5s wide is possible with excellent separation resolution.
L-3500 UV-VIS Detector
The detector keeps excellent linearity up to 2.5 AU absorbance
Proprietary optical design for improved signal to noise ratio.
peak width
resolution peak width
sampling rate
◎ Data rate: 100Hz◎ Linear range: 2.5AU ◎ Detection limit:5×10-9g/mL, with lower dynamic noise and drift◎ Dual-wavelength testing function, higher efficiency for multi-substance analysis
1
2
3 5
6
4
6
15
12
3
4
5
6
7
8
9
10
11
12
13 14
L-3530 Evaporative Light Scattering Detector
With low-temp evaporation technology, the L-3530 can dramatically reduce baseline noise to improve sensitivity.
Determination of 15 underivatized amino-acids via HPLC-ELSD
Chromatographic column: RIGOL AA(250 mm x4.6mm x 5um)Mobile phase: 0.2% Heptafluorobutyric Acid /MeOH; gradient elutionTemperature of drift-tube: 40℃Increment: 7
Determination of gentamycin sulfate injection via HPLC-ELSD
Chromatographic column: RIGOL AA(250 mm x4.6mm x 5um)Mobile phase: 0.2mol/L trifluoroacetic acid solution/MeOH (92/8)Temperature of drift-tube: 50℃Increment: 6
RIGOL L-3530low-temperature technology
other flow-separation methods
to waste liquid
to drift tube to drift tube
nebulization
evaporation
test
◎ Low temperature evaporation, suitable for tests of semi-volatile and thermal-sensitive compounds.◎ Advanced optical head design allows high sensitivity with very low background noise.◎ Proprietary GSF technology offers auto-cleaning functionality, lowers the cost of ownership.◎ Automatic adjustment of gas flow rate
7
L-3320 Autosampler
◎ High precision injector provides excellent accuracy and linearity of sample injection◎ Proprietary needle design significantly reduces sample residue contamination◎ μL pick-up mode achieves zero sample loss
High-precision injector provides excellent accuracy and linearity of sample injection
Peak-area repeatability of 3 compounds with different sampling volume (RSD%)(n=6)
sampling volume from 2.5 to 50uL, correlation coefficients of the 3 compounds are all 0.9999
Proprietary needle design significantly reduces sample residue contamination
Chromatographic column: Rigol C18(250 mm x4.6mm x 5um)Mobile phase: MeOH/Water(90/10)Sampling volume:10μLCross-contamination: 0.005%(based on naphthalene-methanol solution
0.00%0.10%0.20%0.30%0.40%0.50%0.60%0.70%0.80%0.90%1.00%
1 2 5 10 20 50
Peak area %RSD
Peak 1
Peak 2
Peak 3
Sampling volume (μL)
Peak area %RSD
Sampling volume (μL)
Peak 1
Peak 2
Peak 3
Cross-contamination: 0.005%
8
RIGOL Chromatography Workstation
◎ Compliance with FDA 21 CFR Part 11/GMP/GLP
◎ Intelligent chromatographic workstation with integrated powerful instrument controller
◎ Powerful data processing, various integral functions and calculation procedures
◎ Monitoring, processing and calibration are all accessed from intuitive graphical user interface
RIGOLCWS
user
command/parameter
report
report
instrument monitor
signaldigital
communication, A/D converter,
LAN diagnosis/online report/version
updating
expandable performances
PDA,GPC,SST regulatory
compliance 21CFR Part
11
qualification tools
report printing,PDF,
emails
data acquisition
technical support
method setup
RIGOL’s workstation furthers the capability of L-3000 series HPLC system with Analysis solution research and development, instrument control, data acquisition and processing, report generation, and traceable record management with versatile extensibilities.
9
RIGOL chromatographic column
◎ General types of high-inert reversed phase column◎ Competence for acid, alkaline and neutral compounds◎ Good selectivity◎ Excellent performance repeatability
Retention time Symmetry factor Capacity factor
High repeatability of retention time, symmetry & capacity factors between 1st and 1000th injection
after first injection
after 1000 injections
component
1
2 34
after first injectionafter 1000 injections
10
1
2
5
3
4
1
2
4
5
8
10
6
7 913
15 1614
11
123
Application and Solutions
Application case studies
Application
HPLC determination of panax notoginseng saponins
Chromatographic column: RIGOL Compass C18, 5μm, 250 mm x4.6mmMobile phase: water/acetonitrile, gradient elutionColumn temperture: 30℃Wavelength for test: 203 nm
HPLC determination of PAHs in air
Chromatographic column: Rigol 300Å 5μm x4.6 x id 300AMobile phase: A: MeOH, B: water (gradient elution)Column temperture: 30℃Wavelength for test: 254 nm
Pharmaceutical Analysis Food Safety Environmental Protection Chemical Manufacturing
Bio-products Agriculture Education & Research Cosmetics Test
The professional product definition, proprietary design, solid performance and intuitive user interface make the RIGOL L-3000 series HPLC ideal for widely varying applications from pharmaceutical analysis and food safety to environmental monitoring and much more.
RIGOL provides you with customized solutions that help you meet the challenges of today and tomorrow.
11
1
2
4
3 12 3
4
HPLC determination of Phthalate in beverage
Chromatographic column: RIGOL Compass C18, 5μm, 4.6mm x 250mmMobile phase: MeOH- water (gradient elution)Column temperture: room temperatureWavelength for test: 275 nm
HPLC determination of hormone in cosmetics
Chromatographic column: Rigol C-18, 5μm, 4.6mm x 250mmMobile phase: water/acetonitrile (gradient elution)Column temperture: 35℃Wavelength for test: 204 nm
Application Solutions
12
L-3000 Modules and Configuration
Flexible solutions, Solid performance: L-3000 HPLC system features a variety of compatible, extensible and high-performance modules for customized applications.
Solvent O
rganizerD
elivery Pum
pS
ampler
Colum
n Oven
Detector
Isocratic system, configurable with UV-VIS and ELS detector
L-3210
Isocratic Pump
Binary Pump
·8000PSI
L-3400
·precision: ±0.1℃
7725iManual Syringe
L-3320Autosampler
L-3530ELSD
L-3220
·8000PSI·2 pumps integration
L-3240
·8000PSI·quarternary pump;
L-3120
·2-channel degassing
L-3500
UV-VIS detector
·Linear range: 2.5AU
L-3140
·4-channel degassing
Binary system with manual sampler, configurable with UV-VIS and ELS detector
Binary system with autosampler, configurable with UV-VIS and ELS detector
Quarternay system with autosampler, configurable with UV-VIS and ELS detector
·onlinewash ·onlinewash ·onlinewash
L-3100Solvent Organizer
Solvent Organizer
Solvent Organizer
Quarternary Pump
low-temp
evaporation
HIGHsensitivity
low-temp
evaporation
HIGHsensitivity
low-temp
evaporation
HIGHsensitivity
low-temp
evaporation
HIGHsensitivity
Column Oven
13
Solvent Organizer L-3100 Solvent Organizer
L-3120 Solvent Organizer
L-3140 Solvent Organizer
Online WashOnline
Degassing None
None
Standard Configuration
2-channel (480μL/channel) 4-channel (480μL/channel)
Delivery Pump L-3210 Isocratic pump
L-3220 Binary pump
L-3240 Quarternary pump
Flow Rate RangeFlow Rate Accuracy
Flow Rate Precision
Max. Pressure
Pressure PulsationGradient AccuracyGradient Rreproducibility
±0.5% (@1mL/min, water)≤0.1% (@1mL/min, water)(JJG)≤0.07% RSD (@1mL/min, water)(ASTM)
8,000 psi (@0-2mL/min)6,000 psi (@2-5mL/min)3,000 psi (@5-10mL/min)
0.001mL/min – 10.000mL/min (increment 0.001mL/min)
≤1% (@1mL/min, water, backpressure >10Mpa)±0.5%
≤0.15% RSD ≤0.15% RSD
Autosampler L-3320 autosampler
Sampling ModeVolume of Sample Loop
Max. Sampling Volume
Sample Volume
Cross ContaminationLinearity Pressure Limit
Full loop,partial loopfill and μl pick-upstandard:50 μL (optional for 10, 20, 100μL)
Full loop = loop volumePartial loop fill = 1/2 loop volumeμl pick-up =(loop volume- 3 × needle volume)/2
2*48 1.5mL vials(standard)(Optional: 96-hole panel, 384-hole panel, 10mL bottle)
< 0.02%0.99996000 psi (15,000psi sampling valve is optional)
Column Oven L-3400 Column Oven
Temperature Range Room Temp +5℃ - 70℃Temperature Accuracy ± 1.0 ℃Temperature Stability ± 0.1 ℃Column Load 250mm column ×1, 50mm guard column ×1
Performance Parameters of RIGOL L-3000 Series
14
UV-VIS Detector L-3500 UV-VIS Detector
Wavelength 190-800 nmLight Source Deuterium lamp, Tungsten lampSpectral Width 8nmWavelength Accuracy ±1 nmWavelength Precision 0.2 nm
Noise ±0.5×10-5 AU (JJG)
±0.35×10-5 AU (ASTM)
Drift 1×10-4 AU/Hr
Linear Range >5×104 (JJG) >2.5AU (ASTM)Max. Sampling Rate 100 HzFlowcell Pressure 1200 PSIFlowcell Light Path 10 mmFlowcell Volume 12μLDetection Limit 5×10-9 g/mL
ELSD L-3530 Evaporative Light Scattering Detector
Light Source LED 470nmDetective high-sensitive photomultiplier tubeEvaporation Temperature RT - 100 ℃ (increment 1℃)Flow Rate 0.1mL/min – 2.5mL/min (standard)Air Consumption <3.0L/minAir Required >5L/min nitrogen gas or air,2 -4.5Bar
Service & Support Email Address: service.chem@rigol.com
Technical Support and Customer Service
Powerful Lab Test Procedures-DevelopmentRIGOL’s experienced Application Engineer team is dedicated to lab solutions and test procedures development, public hot case study, application optimization, etc. in food safety, pharmacy, biology and environmental monitoring fields. The team also provides the customers with test procedures verification service.
Completed SolutionsRIGOL’s technical support team understands customers needs, provides the customers’with application optimized system configurations, regulations complied test solutions, as well as responsive and quality customer support.
Quality Training ServicesRIGOL offers on-site training service, RIGOL technical support engineers will help the customers better understand the instruments operations and maintenance, provide the customers with the prompt consulting and support on-site and/or remotely.
Comprehensive Customer ServiceRIGOL’s customer-service center is committed to answering customers’ service requirement effectively and professionally.
RIGOL® is the registered trademark of RIGOL technologies, Inc. Product information in this catalogue subject to be changed without notification. For the
latest information about RIGOL’s products, applications and services, please contact RIGOL local office or visit www.rigol.com
All rights reserved, RIGOL Technologies, Inc. 1998-2012. Version: Jan 2013
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