CHEM 213 Experiment: Column Chromatographychemistry.bd.psu.edu/justik/CHEM 213/CHEM 213...

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CHEM213Experiment:ColumnChromatography

Procedure:Youwillbeseparatingthemixturethatresultsfromthereactionbelow:

Inthisparticularexperiment,theoxidationisincompleteandyouhaveamixtureoffluoreneandfluorenonetoseparate.Objectives:

• Separatethetwocomponentsbycolumnchromatography.• Identifyeachcomponent

• Determinethemassofeachcomponent

Step1:Weighout~50mgofthemixture.Useabalanceoutsideofhoodtoeliminatetheindicatedmassfrombeingaffectedbytheairflow.FollowtheprocedurefromWilliamsonasfollows:

CHEM 213 Experiment: Column Chromatographychemistry.bd.psu.edu/justik/CHEM 213/CHEM 213...

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