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Chapter 14
Companion site for Light and Video MicroscopyAuthor: Wayne
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
A video camera converts intensities into voltages..
FIGURE 14-1
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Linear amplification of an electrical signal that represents intensities.
FIGURE 14-2
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Nonlinear amplification of the electrical signal that represents intensities
FIGURE 14-3
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Examples of complex processing of the electrical signal that represents intensities.
FIGURE 14-4
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Taking the first derivative of the electrical signal produces a pseudo-relief image.
FIGURE 14-5
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Taking the second derivative of the electrical signal and subtracting it from the original signal sharpens the image.
FIGURE 14-6
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier.
FIGURE 14-7
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier configured to function as an inverting amplifier.
FIGURE 14-8
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier configured to function as a noninverting amplifier.
FIGURE 14-9
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An equivalent circuit.
FIGURE 14-10
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier configured as an amplifier with unity gain.
FIGURE 14-11
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier configured as a differentiator.
FIGURE 14-12
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier configured as an integrator.
FIGURE 14-13
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Operational amplifiers configured as a logarithmic converter.
FIGURE 14-14
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Operational amplifiers used to return the absolute value of the input.
FIGURE 14-15
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Operational amplifier configured as a low pass filter.
FIGURE 14-16
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Operational amplifier configured as a high pass filter.
FIGURE 14-17
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Operational amplifier configured as a band pass filter.
FIGURE 14-18
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier configured as an adder connection.
FIGURE 14-19
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
An operational amplifier configured as a subtractor connection.
FIGURE 14-20
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Beating the Rayleigh criterion using the offset and gain controls of an analog image processor.
FIGURE 14-21
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
The formation of a digital image.
FIGURE 14-22
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Use Fourier transforms to correct for the degradation of the image produced by the optical system itself.
FIGURE 14-23
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
The convolution filtering process.
FIGURE 14-24
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
Using multipaly-stained beads to put in register images from multiply stained specimens.
FIGURE 14-25
Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved.
FIGURE 14-26
The distortion of reality and the reality of distortion.
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