CastAway: Precast Sequencing System Features and Benefits

CastAway: Precast Sequencing System

Features and Benefits

CastAway:: It’s Easy Easy1. Remove gel from bag.

2. Place gel in sequencing device

3. Slide gel into dryer

4. Analyze results

CastAway: It’s Fast

CastAway: It’s Safe

Unpolymerized acrylamide is a neurotoxin and therefore hazardous to work with, since CastAway gels are provided pre-polymerized, exposure to toxic acrylamide is minimized.

CastAway: It’s Consistent Approximately 15-25% of manually cast

polyacrylamide sequencing gels are discarded due to:

-leaks-bubbles -poor polymerization

CastAway gels are insured by Stratagene’s quality control for perfect gels, every time.

……And Even Gel Drying is And Even Gel Drying is EasierEasier

It is no longer necessary to transfer your gel to filter paper– The gel is fixed to one of the glass plates and

dried directly on the plate

No noisy vacuum pumps that are difficult to maintain– The CastAway dryer uses forced air to dry your

gels in only 30 minutes

CastAwayCastAway System System

ComponentsComponents

Complete CastAway SystemComplete CastAway System100/120v #401098100/120v #401098 230v #401099 230v #401099

Sequencing Device Gel Dryer Fixing Trays (2) Radiation Storage Container Shark-Tooth Comb Set (3 each 20-, 32-, 48-

well combs) High-Voltage Leads Gasket Lubricant

CastAway Sequencing Device

Catalog #401070 Sequencing Device

Dimensions: 48.5 cm H x 30 cm W x 17 cm D Weight 3 kg

Shark-Tooth Comb Set (3 each 20-, 32-and 48-well combs)

High-Voltage Leads Gasket Lubricant

CastAway Cassettes and Kodak BioMax MR Film

CastAway Autoradiography Cassettes #401116 -Compact and lightweight -18 x 43 cm, perfectly fit CastAway gels.

Kodak BioMax MR Film #401117 -The preferred film for high resolution sequencing, sized to fit CastAway gels and cassettes.

CastAway Gel-Loading Syringes

Single-Channel Syringes #401086 -0.2mm diameter needle fits easily into the wells of a CastAway gel

8-Channel Syringes #401087 -For use

with Shark-Tooth comb for 8-channel pipettors

CastAway Silver Staining Kit Novel Enhancing Reagent

provides higher contrast silver staining with lower background than conventional methods

Single use packaging for staining CastAway gels

Easy DNA recovery: bands can be removed from the gel and used directly for reamplification without delays for purifying the gel fragments or film exposure.

Comparison of PAGE data from conventional silver staining method (A) and the CastAway silver staining kit (B)

75mer

50mer

35mer

20mer

1 2 3 4 1 2 3 4

(A) (B)

CastAway Rehydration Kit and Components

Rehydration Kit #401076-10X Rehydration Buffer-Gel Blades (5)-Scraping Trays (2)

10X Rehydration Buffer #401077 Gel Blades (5) #401078 Scraping Trays (2) #401079

CastAway 6% Precast Gels

6% Polyacrylamide Gels #401090-6% polyacrylamide, 7M urea, 1x TBE

6% Nondenaturing Gels #401096 -6% polyacrylamide, 1x TBE

6% Gels w/Preformed Wells #401094 -6% polyacrylamide, 7M urea, 1x TBE -32 preformed wells, compatible with

8-channel pipettors

CastAway 4.5% Precast Gels

4.5% Polyacrylamide Gels #401091-4.5% polyacrylamide, 7M urea, 1x TBE

4.5% Gels w/ Preformed Wells #401092-4.5% polyacrylamide, 7M urea, 1x TBE-32 preformed wells, compatible with 8-channel pipettors

CastAway Long RangerTM Gels

5.5% Long Ranger Gels #401097-5.5% Long Ranger gel, 8.3M urea,

1x TBE-Up to 30% longer reads compared

to standard gel matrix

CastAway 12% Precast Gels

12% Polyacrylamide Gels #401093 -12% polyacrylamide, 7M urea, 1x TBE

-32 preformed wells, compatible with 8-channel pipettors

CastAway 8% Precast Gels

8% Polyacrylamide Gels #401088 -8% polyacrylamide, 7M urea, 1x TBE -32 preformed wells, compatible with 8-channel pipettors

CastAway Gels for the CastAway Gels for the LI-COR SystemLI-COR System

The first precast gels for automated sequencing and genetic analysis

25cm gels for the LI-COR IR2 system Run up to 32 sequencing samples or 128

genotyping lanes Gels were developed specifically for use on the

LI-COR system and will not fit the CastAway sequencing device

Automated sequencing and background Automated sequencing and background fluorescencefluorescence

It is unlikely that these precast gels could be used with visible fluorescence automated DNA sequencers such as the ABI PRISMR 377 since the system sensitivity would be lower due to significant emission of background fluorescence by the soda-lime plates.

With the LI-COR system, by using infrared radiation, the background fluorescence due to the soda lime glass of the precast gel is greatly reduced.

Applications:

It’s Not Just For Sequencing

Select the Right Gel for Your ApplicationSelect the Right Gel for Your Application

Application

6% gel, 7Murea w/ orw/o pre-formed wells

6% non-denaturinggel

4.5% gel, 7Murea w/ orw/o pre-formed wells

8% gel, 7Murea w/pre-formed wells

12% gel, 7Murea w/ pre-formed wells

5.5% LongRanger gel,8.3M urea

Sequencing Silver staining DifferentialDisplay/RAP PCR

DNAFingerprinting AFLP SSCP RNAseProtection Assay DNAFootprinting PrimerExtensions OligonucleotideAnalysis MicrosatelliteDNA Analysis

3535S SequenceS Sequence Silver Stained SequenceSilver Stained Sequence

Sequencing

Differential Display/RAP PCR

Figure 1 Figure 2

Figure 3

Figure 1: Differentially displayed products on 6% CastAway gel.

Figure 2: Products to be cut out & reamplified are outlined on the autorad

Figure 3: Reamplified products

Multiplexed PCR ReactionMultiplexed PCR Reaction

Multiplexed PCR reactions were run on a CastAway 8% gel and analyzed using Hitachi’s FMBioII Scanner. The pattern of banding, corresponding to family specific CDR3 length polymorphisms allows comparison of T cell receptor utilization by peripheral blood lymphocytes with that of breast tumor-infiltrating lymphocytes.

RAP PCR Kit control reactions performed

with HepG2 mRNA and C2/C3 primer sets

4.5% CastAway gel w/ 32 pre-formed wells

NEWNEW SmartPower™ 4000 SmartPower™ 4000 Power SupplyPower Supply Catalog # 400690 100/120 Volt Catalog # 400691 230 Volt CE Marked Catalog # 400692 230 Volt non-CE Marked

SmartPower™ 4000 FeaturesSmartPower™ 4000 Features

All-purpose: broad voltage and amperage control ranges make this a multifunctional power supply for both horizontal and sequencing gels- unique feature!

Temperature Control: built-in StrataTherm® temperature controller

Multiple power outputs: Four sets of output jacks enable multiple gels to be run simultaneously

SmartPower™ 4000 FeaturesSmartPower™ 4000 Features

Independent Power Output Monitoring: Current and power can be monitored on each output jack independently- unique feature!

Programmable: the user can create and save commonly used power parameters as individual programs

Timer Control: built-in timer for automatic program start or stop

SmartPower 4000

BioRad PowerPac 3000

E-C Apparatus EC4000P

Pharmacia EPS 3500 XL

Gibco BRL Model 40001P

Voltage 25-4000V 25-3000V 4000V 3500V 4000VCurrent 1-450mA 1-400mA 300mA 150mA 300mAPower 1-450W 1-400W 300W 100W 300WTemp control Yes Yes No No NoTimer Yes Yes Yes Yes YesPower outputs 4 4 4 2 4Programmable Yes Yes Yes Yes YesOutput monitoring Independent

non-Independent

non-Independent

non-Independent

non-Independent

Price 2,495.00$ 1,795.00$ 2,250.00$ 2,295.00$ 2,495.00$

Joule BoxJoule BoxTMTM

Mini Gel Electrophoresis UnitMini Gel Electrophoresis Unit Horizontal electrophoresis device with built-in, mini

power supply Versatile - for both agarose and polyacrylamide gels Gel casting set for pouring up to

6 gels simultaneously CE Marked/UL approved 4 comb sizes for various

loading volumes

New Joule Box AccessoriesNew Joule Box Accessories 10cm Casting Set

Run one 10cm gel or vary comb placement for several shorter runs

Shaded comb insertion areas and gel migration distance lines for easier sample loading and quick determination of run distance

Preparative Comb Set Several comb formats, including large

wells for loading preparative samples

Joule BoxJoule BoxTMTM Vs. The Competition Vs. The Competition

The Joule Box apparatus contains an anti-fogging reagent rather than ventilation slits to prevent condensation inside lid. This technology produces a system which is much safer to run & allows it to be CE Marked.

Joule Box casting trays feature shaded comb insertion areas and migration distance index lines to easily determine gel running distance at a glance.

EliminatorEliminatorTMTM

Dye Removal SystemDye Removal System Up to 99% removal of ethidium

bromide dye from buffers Buffers can then be disposed of without

environmental risk Each Eliminator sleeve can purify 20

liters of liquid No vacuum manifold or pre-

equilibration needed, simply pour buffer into sleeve and allow to drain by gravity flow

Researchers need to be educated– Although there are no environmental standards

for EtBr waste disposal, Ethidium Bromide is a MUTAGEN, and should be disposed of properly - with the ELIMINATOR Dye Removal System!!

Focus on selling to Environmental Health and Safety Departments at all major institutions

Make the Eliminator the standard for Ethidium Bromide waste disposal

LC50 Index = 0.00 (cut off level = 500) - based on testing by Del Mar Analytical, Feb. 1999

EliminatorEliminatorTMTM vs. The vs. The CompetitionCompetition