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Bell Work1.What does a pedigree show?
2.Look at these two nucleotide sequences: ATTGCGCCGTA and ATTGCGCAGTA. What type of mutation is shown in the second sequence?
3.What is the mutation called when a piece of a chromosome breaks off and reattaches backwards?
Tuesday, January 15, 2013
Bell Work1.What does a pedigree show?
2.Look at these two nucleotide sequences: ATTGCGCCGTA and ATTGCGCAGTA. What type of mutation is shown in the second sequence?
3.What is the mutation called when a piece of a chromosome breaks off and reattaches backwards?
inheritance of a trait
Tuesday, January 15, 2013
Bell Work1.What does a pedigree show?
2.Look at these two nucleotide sequences: ATTGCGCCGTA and ATTGCGCAGTA. What type of mutation is shown in the second sequence?
3.What is the mutation called when a piece of a chromosome breaks off and reattaches backwards?
substitution
inheritance of a trait
Tuesday, January 15, 2013
Bell Work1.What does a pedigree show?
2.Look at these two nucleotide sequences: ATTGCGCCGTA and ATTGCGCAGTA. What type of mutation is shown in the second sequence?
3.What is the mutation called when a piece of a chromosome breaks off and reattaches backwards?
substitution
inheritance of a trait
inversion
Tuesday, January 15, 2013
Karyotype Quiz• List 2 things karyotypes identify
• Define autosome
• Sex chromosomes for male? female?
• Total number of chromosomes for humans? pairs?
• Match the following:
• 1. Down’s syndrome
• 2. Edward’s syndrome
• 3. Crit du chat
• 4. Kleinfelter’s syndrome
• 5. Fragile X syndrome
a. #5 Chromosome upper arm is brokenb. Extra X chromosome (XXY); only in malesc. Extra #21 chromosomed. X chromosome appears to be brokene. Extra #18 chromosome
Tuesday, January 15, 2013
Identify the sex and explain the disorder
Tuesday, January 15, 2013
DNA Finger Printing
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction:
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction: : DNA is separatedfrom everything else
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction: : DNA is separated
2.Because DNA is too long, it is ___ into ______________ using restriction enzymes.
from everything else
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction: : DNA is separated
2.Because DNA is too long, it is ___ into ______________ using restriction enzymes.cut
from everything else
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction: : DNA is separated
2.Because DNA is too long, it is ___ into ______________ using restriction enzymes.cut smaller pieces
from everything else
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction: : DNA is separated
2.Because DNA is too long, it is ___ into ______________ using restriction enzymes.cut smaller pieces
from everything else
3.Fragments are _________ by gel electrophoresis.
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction: : DNA is separated
2.Because DNA is too long, it is ___ into ______________ using restriction enzymes.cut smaller pieces
from everything else
3.Fragments are _________ by gel electrophoresis.
separated
Tuesday, January 15, 2013
DNA Finger Printing1.DNA Extraction: : DNA is separated
2.Because DNA is too long, it is ___ into ______________ using restriction enzymes.cut smaller pieces
from everything else
3.Fragments are _________ by gel electrophoresis.
separated
4.The patterns on the gel can be compared.
Tuesday, January 15, 2013
Gel Electrophoresis
Tuesday, January 15, 2013
Gel ElectrophoresisDNA fragments are placed on a special gel, and
separated using an electric current.
Tuesday, January 15, 2013
Gel ElectrophoresisDNA fragments are placed on a special gel, and
separated using an electric current.
• Smaller pieces move towards the bottom quicker.
Tuesday, January 15, 2013
Gel ElectrophoresisDNA fragments are placed on a special gel, and
separated using an electric current.
• Smaller pieces move towards the bottom quicker.• Bands can be compared to determine a match.Tuesday, January 15, 2013
Gel ElectrophoresisDNA fragments are placed on a special gel, and
separated using an electric current.
Tuesday, January 15, 2013
Gel ElectrophoresisDNA fragments are placed on a special gel, and
separated using an electric current.
Tuesday, January 15, 2013
Gel ElectrophoresisThere are two types of questions you might see.
Tuesday, January 15, 2013
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
Tuesday, January 15, 2013
___ ___ ___ ___ ___ ___ ___ ___ ___
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
Evid
ence
Susp
ect
1
Susp
ect
2
Tuesday, January 15, 2013
___ ___ ___ ___ ___ ___ ___ ___ ___
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
Evid
ence
Susp
ect
1
Susp
ect
2
Tuesday, January 15, 2013
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
Perfect match
Tuesday, January 15, 2013
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
PaternityWho’s the daddy?
Perfect match
Tuesday, January 15, 2013
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
PaternityWho’s the daddy?
___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
Perfect match
Tuesday, January 15, 2013
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
PaternityWho’s the daddy?
___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
Perfect match
Tuesday, January 15, 2013
Gel ElectrophoresisThere are two types of questions you might see.
SuspectWho’s the killer?
PaternityWho’s the daddy?
Perfect matchClosest match, because
only half the DNA comes from the father
Tuesday, January 15, 2013
Who’s the killer?
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
Tuesday, January 15, 2013
Who’s the killer?
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
Tuesday, January 15, 2013
Who’s the killer?
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
Tuesday, January 15, 2013
Who’s the killer?
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
Tuesday, January 15, 2013
Who’s the killer?
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Evid
ence
Susp
ect
1
Susp
ect
2
Tuesday, January 15, 2013
Who’s the daddy?
___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
Tuesday, January 15, 2013
Who’s the daddy?
___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
Tuesday, January 15, 2013
Who’s the daddy?
___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
Tuesday, January 15, 2013
Who’s the daddy?
___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
Tuesday, January 15, 2013
Who’s the daddy?
___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Chi
ld
Dad
dy 1
Dad
dy 2
Tuesday, January 15, 2013
Practice1. A condition is autosomal recessive. The pedigree shows the inheritance of this condition in the family. Which of the following is the likely genotype of individual 1?a. eeb. Eec. XeYd. XEXE
Tuesday, January 15, 2013
Practice1. A condition is autosomal recessive. The pedigree shows the inheritance of this condition in the family. Which of the following is the likely genotype of individual 1?a. eeb. Eec. XeYd. XEXE
Autosomal: not a sex chromosome
Tuesday, January 15, 2013
Practice2. The pedigree shows the inheritance of a genetic condition over two generations. Which set of numbered individuals has the greatest chance of producing a child that is affected by this condition?a. 1b. 2c. 3d. 4
Tuesday, January 15, 2013
Practice3. Look at these two nucleotide sequences: ATTGCGCCGTA & ATTGCGCGTA. What type of mutation is shown in the second sequence?a. substitutionb. insertionc. deletiond. radiation
Tuesday, January 15, 2013
Practice4. Which of the following would be least likely to cause a gene mutation?a. exposure to x-ray radiationb. exposure to ultraviolet radiationc. exposure to tobacco smoked. exposure to loud noises
Tuesday, January 15, 2013
Practice5. During meiosis, part of chromosome 5 breaks off and attaches to chromosome 6. What is this mutation called?a. deletionb. substitutionc. duplicationd. translocation
Tuesday, January 15, 2013
Practice6. What can a karyotype be used for?a. predict genetic disorders in future generationsb. show an abnormal number of chromosomesc. prove paternityd. link a suspect with a crime scene
Tuesday, January 15, 2013
Practice7. What does a gene mutation often result in?a. incorrect coding of a proteinb. a more serious chromosome mutationc. a recessive trait passed on to offspringd. a dominant trait passed on to offspring
Tuesday, January 15, 2013
Practice8. The DNA fingerprint results from four dogs and a puppy are shown. If the puppy is to be registered, the father must be known. Which of the following dogs would be the puppy’s father?a. Dog #1b. Dog #2c. Dog #3d. Dog #4
Tuesday, January 15, 2013
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