Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile...

Aspergillus

Classic Diagnostic

Dr. Patricio Godoy Martínez

Universidad Austral de Chile

pgodoymartinez@gmail.com

AspergillusAspergillus

Ubiquitous, commonly ocurring in soil, water, and decaying vegatation.

Reservoirs in hospitals; unfiltered air, ventilation system, carpeting, food, water distribution system, etc.

Cause invasive and allergic disease in humans and other animals.

Cause plant and food spoilage and produce mycotoxins.

Classification of Aspergillus. Classification of Aspergillus. ~260 ~260 especies, especies, 38 have caused disease38 have caused disease

Balajee & Marr,2006. Future Microbiology, 1:435-445.

Geiser 2009. Medical Mycology, 47:S21-S26

Sexual stages associated with the Sexual stages associated with the genus genus AspergillusAspergillus

AspergillusAspergillus

Direct Indirect

Mycology

Diagnostics

AspergillusAspergillus

Microscopic examination

Microscopic examination

ColonyColonyDetection of Ag and A.N

Detection of Ag and A.N

Direct MethodsDirect Methods

ColorationHistopathology

ColorationHistopathology

Direct microscopic examinationDirect microscopic examination

hyphaes hyalines septate.hyphaes hyalines septate.

Vitale R

Histopathology: H&EHistopathology: H&E

Vitale R

Histopathology: GrocottHistopathology: Grocott

Vitale R

IdentificationIdentification

Macro-microscopic observation of Macro-microscopic observation of colonies colonies

Morphological characterMorphological character

COLUMNAR

RADIATE

Morphological characterMorphological character

Josep Guarro

Aspergillus fumigatusAspergillus fumigatus

Colonies black blue-green, consisting of a dense felt of conidiophores. www. pgodoy. com

Aspergillus fumigatusAspergillus fumigatus

Conidial heads columnar. Conidiogenous cells uniseriate. Conidiophore stipes smooth-walled often green in the upper part. Conidia verrucose. www.pgodoy.com

www.aspergillus.man.ac.uk

Neosartorya

Anamorphs closely related to A. fumigatus.

Teleomorphs only differentiated by subtle differences in ascospores ornamentation.

Thermophilic.

Neosartorya

N. fischeri

N. pseudofischeri

N. hiratsukae

Neosartorya

Osteomyelitis

Keratitis

Endocarditis

Peritonitis

Cerebral infection

Pulmonary infection

Disseminated infection

Neosartorya pseudofischeri

Neosartorya hiratsukaeNeosartorya hiratsukae

Josep Guarro

N. pseudofischeriN. pseudofischeri

N. hiratsukaeN. hiratsukae

Josep Guarro

Josep Guarro

Neosartorya

Underdiagnosed (regarded as contaminants in the lab)

Difficult to identify at species level.

Antifungal susceptibility similar to A. fumigatus ?

Little experience in the treatment. Amphotericin B and itraconazole

Josep Guarro

Aspergillus nigerAspergillus niger

Colonies black, consisting of a dense felt of conidiophores. www. pgodoy. com

Aspergillus nigerAspergillus niger

Conidial heads radiate. Vesicles subspherical, conidiogenous cells biseriate. Conidia brown, ornamented. www.aspergillus.man.ac.uk

Aspergillus flavusAspergillus flavus

Colonies yellowish-green, consisting of a dense felt of conidiophores.

www.aspergillus.man.ac.uk

www.pgodoy.com

Aspergillus flavusAspergillus flavus

Conidial heads radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. Sclerotia may be present.

www.aspergillus.man.ac.uk

Aspergillus parasiticusAspergillus parasiticus

R. Salim & R. Runco

Colonies yellowish-brown, consisting of a dense felt of conidiophores.

conidiophores of varying lengths, wall hyaline, thick, with ornamentation equinulada, spherical vesicles, uni or biseriate (24-25 m), completely covering the fiálides gallbladder. Conidia globose or subglobosos warted (2.5-3 m) with thin walls (20, 21).

Aspergillus tamariiAspergillus tamarii

Conidial heads compact and spherical or loosely radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate.

www.aspergillus.man.ac.uk

Aspergillus orizaeAspergillus orizae

Conidial heads radiate to loosely columnar. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia smooth-walled to roughened.

www.aspergillus.man.ac.uk

Aspergillus terreusAspergillus terreus

Colonies yellowish-brown to cinnamon-brown, consisting of a dense felt of conidiophores.

www.aspergillus.man.ac.uk

Aspergillus terreusAspergillus terreus

Conidial heads densely columnar. Conidiogenous cells biseriate. Conidiophore stipes smooth-walled hyaline. Conidia smooth walled, striate hyaline.

www.aspergillus.man.ac.uk

Aspergillus nidulansAspergillus nidulans

Colonies growing rapidly, green, cream-buff or honey-yellow, reverse dark purple.

Vitale R

Aspergillus nidulansAspergillus nidulans

Conidial heads short, columnar. Conidiogenous cells biseriate. Conidiophore stipes brownish. Conidia spherical, rugulose, subhyaline, green.

Vitale R

Aspergillus clavatusAspergillus clavatus

Colonies growing rapidly, bluish-green, consisting of a dense felt of conidiophores.

Conidial heads radiate, later splitting into several columns. Conidiogenous cells uniseriate. Conidiophore stipes smooth. Vesicle clavate. Conidia smooth-walled hyaline.

Aspergillus clavatusAspergillus clavatus

www.pgodoy.com

INFOCUS 2010 - CHILEINFOCUS 2010 - CHILE