View
2
Download
0
Category
Preview:
Citation preview
Supplementary Material
Drug-loaded titanium dioxide nanoparticle coated with tumor targeting polymer as a
sonodynamic chemotherapeutic agent for anti-cancer therapy
Seonil Kima, Sooseok Imb, Eun-Yeong Parkc, Junseok Leea, Chulhong Kimd, Tae-il Kime,*, and
Won Jong Kima,*
a Department of Chemistry, Pohang University of Science and Technology (POSTECH),
Jigok-ro 64, Nam-gu, Pohang 37666, Republic of Korea
*Corresponding author
Email: wjkim@postech.ac.kr
b School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science
and Technology (POSTECH), Jigok-ro 64, Nam-gu, Pohang 37666, Republic of Korea
c Department of Electrical Engineering, Pohang University of Science and Technology
(POSTECH), Pohang, Republic of Korea
d Departments of Creative IT Engineering and Electrical Engineering, Pohang University of
Science and Technology (POSTECH), Pohang, Republic of Korea
e Department of Biosystems & Biomaterials Science and Engineering, College of Agriculture
and Life Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826,
Republic of Korea
1
O
OO O513
+BOH
OH
O
x
O
OHOOCHN
yCOOHO OH
H2N
BOH
HOPoly(methyl vinyl ether-alt-
maleic anhydride)(pMVMA)
3-aminophenylboronic acid
(PBA)
1. DMSO, 12 h
2. DIalysis
Poly(pheny boronic acid)(pPBA)
Figure S1. Synthetic procedure of poly(phenylboronic acid) (pPBA).
Figure S2. 1H-NMR spectra of pPBA The molar ratio of conjugated PBA was calculated by
1H NMR (yield: 93%). 1H NMR (Methanol-d4, 500 MHz): 1.6–2.3 (m, –CH2–, 2H); 2.8–3.2
(m, anhydride, 2H); 3.3-3.5 (m, –OCH3, 3H); 3.6–4.0 (m, –CH–, 1H); 7.0–7.8 (m, Ph, 4xH).
2
Figure S3. FT-IR spectra of pPBA, pMVMA, and PBA-NH2.
Figure S4. (A) Schematic illustration of pMA@TNP-DOX and pPBA@TNP formation. (B)
DLS size measurement of pMA@TNP-DOX and pPBA@TNP.
3
Figure S5. (A) Fluorescence spectra DOX and DOX-TNP with 1 mM DOX equivalence at
ex = 495 nm. (B) Fluorescence spectra of pPBA and pPBA@TNP-DOX with 1 mM PBA at
ex = 300 nm.
Figure S6. Zeta potential measurement of pPBA, TNP, TNP-DOX, and pPBA@TNP-DOX.
4
Figure S7. Cytotoxicity of ultrasound irradiation in MCF-7 cell line and MDA-MB-231 cell
line (3 W·cm-2, 1.5 MHz, duty cycle: 20%) analysed by MTT assay.
Figure S8. Flow cytometry of MDA-MB-231 cell line treated with pPBA@TNP-DOX.
pPBA, pMA@TNP-DOX, and pPBA@TNP-DOX. For competition assay, pPBA@TNP-
DOX was co-incubated with PBA-NH2.
5
Figure S9. Serum stability of bared TNP and pPBA@TNP-DOX.
DOXpP
BATNP
pPBA@
TNP-DOX
0
20
40
60
Hem
olys
is (%
)
Figure S10. Quantitative hemolysis assay using 1 mM DOX, pPBA, TNP, and pPBA@TNP-
DOX in vitro.
6
Figure S11. Ex vivo DOX accumulation. Quantitative analysis of DOX content (total
fluorescence intensity of DOX from each organ) in each organ after treatment of free DOX,
pMA@TNP-DOX and pPBA@TNP-DOX (n=3, *P < 0.05).
Figure S12. Body weight changes of mice for 21 days.
7
Figure S13. Representative tumor images at day 21 after i.v. injection
8
Recommended