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nanomaterials
Review
Applications of Nanocellulose/NanocarbonComposites: Focus on Biotechnology and Medicine
Lucie Bacakova 1,*, Julia Pajorova 1, Maria Tomkova 2, Roman Matejka 1 , Antonin Broz 1,Jana Stepanovska 1, Simon Prazak 1, Anne Skogberg 3 , Sanna Siljander 4 and Pasi Kallio 3
1 Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy ofSciences, Videnska 1083, 14220 Prague, Czech Republic; Julia.Pajorova@fgu.cas.cz (J.P.);Roman.Matejka@fgu.cas.cz (R.M.); Antonin.Broz@fgu.cas.cz (A.B.); Jana.Stepanovska@fgu.cas.cz (J.S.);Simon.Prazak@fgu.cas.cz (S.P.)
2 Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2,94976 Nitra, Slovakia; xtomkovam2@uniag.sk
3 BioMediTech Institute and Faculty of Medicine and Health Technology, Tampere University,Korkeakoulunkatu 3, 33014 Tampere, Finland; anne.skogberg@tuni.fi (A.S.); pasi.kallio@tuni.fi (P.K.)
4 Automation Technology and Mechanical Engineering, Faculty of Engineering and Natural Sciences, TampereUniversity, Korkeakoulunkatu 6, 33720 Tampere, Finland; sanna.siljander@tuni.fi
* Correspondence: Lucie.Bacakova@fgu.cas.cz; Tel.: +420-2-9644-3743
Received: 27 December 2019; Accepted: 21 January 2020; Published: 23 January 2020�����������������
Abstract: Nanocellulose/nanocarbon composites are newly emerging smart hybrid materialscontaining cellulose nanoparticles, such as nanofibrils and nanocrystals, and carbon nanoparticles,such as “classical” carbon allotropes (fullerenes, graphene, nanotubes and nanodiamonds), or othercarbon nanostructures (carbon nanofibers, carbon quantum dots, activated carbon and carbon black).The nanocellulose component acts as a dispersing agent and homogeneously distributes the carbonnanoparticles in an aqueous environment. Nanocellulose/nanocarbon composites can be prepared withmany advantageous properties, such as high mechanical strength, flexibility, stretchability, tunablethermal and electrical conductivity, tunable optical transparency, photodynamic and photothermalactivity, nanoporous character and high adsorption capacity. They are therefore promising for awide range of industrial applications, such as energy generation, storage and conversion, waterpurification, food packaging, construction of fire retardants and shape memory devices. They alsohold great promise for biomedical applications, such as radical scavenging, photodynamic andphotothermal therapy of tumors and microbial infections, drug delivery, biosensorics, isolation ofvarious biomolecules, electrical stimulation of damaged tissues (e.g., cardiac, neural), neural andbone tissue engineering, engineering of blood vessels and advanced wound dressing, e.g., withantimicrobial and antitumor activity. However, the potential cytotoxicity and immunogenicity of thecomposites and their components must also be taken into account.
Keywords: nanofibrillated cellulose; cellulose nanocrystals; fullerenes; graphene; carbon nanotubes;diamond nanoparticles; sensors; drug delivery; tissue engineering; wound dressing
1. Introduction
Nanocellulose/nanocarbon composites are hybrid materials containing cellulose and carbonnanoparticles. Integration of nanocarbon materials with nanocellulose provides functionality ofnanocarbons, using an eco-friendly, low-cost, strong, dimension-stable, nonmelting, nontoxic andnonmetal matrix or carrier, which alone has versatile applications in industry, biotechnology andbiomedicine (for a review, see [1,2]). In addition to its advantageous combination with nanocarbonmaterials, nanocellulose is an appealing material for biomedical applications due to its tunable chemical
Nanomaterials 2020, 10, 196; doi:10.3390/nano10020196 www.mdpi.com/journal/nanomaterials
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properties, nonanimal origin, and resemblance to biological molecules in dimension, chemistry andviscoelastic properties, etc. [3–6].
Cellulose nanomaterials include cellulose nanofibrils (CNFs) and cellulose nanocrystals (CNCs) [3].CNFs are manufactured using either a bottom-up or a top-down approach. The bottom-up approachinvolves bacterial (Gluconacetobacter) biosynthesis to obtain bacterial cellulose (BC), while, in thetop-down method, cellulosic biomass from plant fibers is disintegrated into smaller CNFs [7] thatcontain amorphous and crystalline regions [3]. The fibrillation of cellulose is achieved using mechanicalforces, chemical treatments, enzymes or combinations of these. After fibrillation, the width of CNFsis typically between 3 and 100 nm, and the length can be several micrometers [8]. Separation of thecrystalline parts from the amorphous regions of the fibers or fibrils to obtain CNCs typically requiresacid hydrolysis, which destroys the amorphous regions [9]. Entangled CNFs are longer, while CNCspossess shorter needle- or rod-like morphology with a similar diameter and a more rigid molecule dueto their higher crystallinity [3,9]. In general, the properties of nanocelluloses are variable and dependon their origin, type, processing, pretreatments and functionalization. Integration with other materials,as well as fabrication of the final product, further affects the properties of the resulting composite orhybrid structure.
Carbon nanoparticles include fullerenes (usually C60), graphene-based particles (graphene,graphene oxide, reduced graphene oxide, graphene quantum dots), nanotubes (single-walled,double-walled, few-walled or multi-walled) and nanodiamonds (for a review, see [10–20]). The mostfrequently used nanocellulose/nanocarbon composites contain graphene or carbon nanotubes, whilecomposites of nanocellulose with nanodiamond, and particularly with fullerenes, are less frequentlyused. Other carbon nanostructures, which are less frequently used in nanocellulose/nanocarboncomposites, at least for biomedical applications, include carbon nanofibers [21–25], carbon quantumdots [26–28] activated carbon [29,30] and carbon black [31–33].
Nanocellulose/nanocarbon composites can be prepared in one-dimensional (1D), two-dimensional(2D) or three-dimensional (3D) forms. 1D composites are represented, for example, by C60 fullerenesgrafted onto cellulose nanocrystals that have undergone amination or oxidation [34,35]. 2D compositesare represented by films, which can be self-standing or supported, i.e., in the form of free-standingmembranes [29,36–41] or in the form of coatings deposited on bulk materials [33,42]. The films can beformed by depositing carbon nanoparticles on a nanocellulose layer [43,44]. More frequently, however,they are fabricated from aqueous dispersions of nanocellulose and carbon nanoparticles [39,42].It should be pointed out that cellulose nanoparticles are excellent dispersive agents for carbonnanoparticles, as they prevent the aggregation of these nanoparticles and maintain them in long-termstable homogeneous suspensions without the need to subject them to chemical functionalization [45,46].Suspensions of cellulose and carbon nanoparticles are also starting materials for the creation of3D nanocellulose/nanocarbon composites in the form of aerogels, foams or sponges [45,47–50]. Inaddition, composite 3D scaffolds, especially for tissue engineering and for regenerative medicine,can be fabricated by 3D printing using bioinks based on cellulose and carbon nanoparticles [51,52].Both 2D composites and 3D composites can also be created by adding carbon nanoparticles tocultures of cellulose-producing bacteria, such as Gluconacetobacter xylinus. These nanoparticles are thenincorporated into bacterial nanocellulose in situ during its growth [53–57]. Another approach is viathe electrospinning or wet spinning of solutions containing cellulose and carbon nanoparticles [58–60].
Nanocellulose/nanocarbon composites exhibit several more advantageous properties thanmaterials containing only cellulose nanoparticles or only carbon nanoparticles. Adding carbonnanoparticles to nanocellulose materials can further increase their mechanical strength [59,61].At the same time, the presence of nanocellulose promotes the flexibility and stretchability of thematerials [52,62,63]; for a review, see [64]. Adding graphene, carbon nanotubes or boron-dopeddiamond nanoparticles endows nanocellulose materials with electrical conductivity [39,50,57,65,66].Other advantageous properties of nanocellulose/nanocarbon composites include their thermalstability [67–69], tunable thermal conductivity and optical transparency [48,57,70], intrinsic
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fluorescence and luminescence [26,71,72] photothermal activity [56], hydrolytic stability [61],nanoporous character and high adsorption capacity [49,61]. Nanocellulose/nanocarbon compositescan therefore be used in a wide range of industrial and technological applications, such as waterpurification [22,29,43,49,54,56,61,73–76], the isolation and separation of various molecules [22,74,77–79],energy generation, storage and conversion [21,23,44,47,64,80–85], biocatalysis [86], foodpackaging [67–69,87], construction of fire retardants [48], heat spreaders [70] and shape memorydevices [38,88–90]. These composites are also used as fillers for various materials, usually polymers, inorder to improve their mechanical, electrical and other physical and chemical properties [67–69,87,91].
In addition, nanocellulose/nanocarbon composites are promising for biomedical applications,though these applications are less frequent than industrial applications. Biomedical applicationsinclude radical scavenging [34,92], photothermal ablation of pathogenic bacteria [93], photodynamicand combined chemophotothermal therapy against cancer [35,94], drug delivery [16,28,65,72,95–97],biosensorics [31–33,63,66,71,91,98–104], and particularly tissue engineering and wound dressings.Hybrid materials containing nanocellulose and nanocarbons stimulated the growth and osteogenicdifferentiation of human bone marrow mesenchymal stem cells [37,59]. They provided good substratesfor the attachment, growth and differentiation of SH-SHY5Y human neuroblastoma cells [51] and PC12neural cells, particularly under electrical stimulation [105]. They enhanced the outgrowth of neuritesfrom rat dorsal root ganglions in vitro and stimulated nerve regeneration in rats in vivo [106]. They alsopromoted the growth of vascular endothelial cells, enhanced angiogenesis and arteriogenesis in a chickchorioallantoic membrane model [107], and improved cardiac conduction when applied to surgicallydisrupted myocardium in dogs [52]. In addition, these materials supported the growth of humandermal fibroblasts [108] and mouse subcutaneous L929 fibroblasts [58,62], promoted wound healingin vivo in mice [109] and showed an antibacterial effect [30]. These materials are therefore promisingfor bone, neural and vascular tissue engineering, for creating cardiac patches and for advanced wounddressings. The biomedical applications of nanocellulose/nanocarbon composites are summarized inTable 1.
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Table 1. Biomedical applications of nanocellulose/nanocarbon composites.
Application Nanocellulose/Nanocarbon Composites Containing:
Fullerenes Graphene CNTs Nanodiamonds Others
Radical scavenging NH2-CNC/C60 [34];CNC/C60(OH)30 [92]
Photodynamic cancer therapy TEMPO-oxidizedCNC/C60-NH2 [35]
Photothermal,chemo-photothermal therapy
Bacteria: [93]
Cancer: [94]
Drug delivery Anticancer drugs (doxorubicin)[72,95,96] Anticancer and other drugs [16] Anticancer drugs
(doxorubicin) [97]Carbon quantum dots: Anticancer
drugs (temozolomide) [28]
(Bio)sensors
Electrochemical: cholesterol [98];glucose and bacteria [110]; avian
leucosis virus [111]; organic liquids[112]
Electrochemical:ATP metabolites [102]; oxygen [84]
Electrochemical: Biotin[66]
Carbon black: Electrochemicalaptasensor for S. aureus [32];
electrochemical sensor for H2O2[33]
Piezoelectric: strain, human motion[63,99,113]
Piezoresistance and thermoelectric-based:pressure and temperature [103];
pressure [17];strain, human motion [90,91,114];
humidity, human breath [104]
Carbon black: Strain, humanmotion [31,33]
Optical:oxygen and temperature
[115]; oxygen [116]
Optical: SERS: bilirubin [100];Fluorescence: laccase [71]
Carbon quantum dots: opticalsensor for biothiols [26]
Acoustic: ammonia [101]
Isolation of biomolecules Histidine-rich proteins, hemoglobin[77]; bovine serum albumin [79]
Electrical stimulation oftissues
Cardiac tissue [52]; neural tissue[106]
Tissue engineering (TE)General cell biocompatibility [68,69,87];
bone TE [37,59]; neural TE [105];vascular TE [107]
Neural tissue engineering [51]; TEin general [117]
Wound dressing/healing Polysaccharides/fullereneC60 derivatives [118]
Human dermal fibroblasts in vitro[108]; mouse model in vivo [109]
L929 fibroblasts in vitro[58,62]; HeLa cells in vitro,
wound dressingsdelivering doxorubicin
[97]
Activated carbon: antibacterialwound dressing [30]
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This review summarizes recent knowledge on the types, properties and applications ofnanocellulose/nanocarbon-based hybrid materials, particularly in biotechnology, biomedicine andtissue engineering, and reports on the experience acquired by our group.
2. Nanocellulose/Fullerene Composites
2.1. Characterization of Fullerenes
Fullerenes are spheroidal cage-like molecules composed entirely of carbon atoms (Figure 1a).Fullerenes with 60 and 70 carbon atoms (C60 and C70) are the most stable molecules, and they aretherefore most frequently used in industrial and biomedical applications. Fullerenes were discoveredin 1985 by Sir Harold Walter Kroto (1939–2016) and his co-workers Richard Smalley, Robert Curl, JamesHeath and Sean O’Brien. Kroto, Smalley and Curl were awarded the Nobel Prize in 1996. Fullereneswere named after Richard Buckminster “Bucky” Fuller (1895–1983), an American architect, designer,futurist, inventor, poet and visionary, who designed his geodesic dome on similar structural principles(for a review, see [12,13,18,119,120]). Fullerenes are carbon nanoparticles with diverse biologicalactivities. This is due to the fact that they can act as either acceptors or donors of electrons (for areview, see [121]). The acceptor activity can lead to oxidative damage to cell components, such asDNA, cell membrane, mitochondria and various enzymes, to the activation of inflammatory reactions,and to cell apoptosis. These harmful effects of fullerenes can, however, be utilized for photodynamictherapy against tumors and pathogenic microorganisms (for a review, see [122]). The electron donoractivity is associated with quenching oxygen radicals, which can be used in protecting skin against UVirradiation, in anti-inflammatory therapy against osteoarthritis, in cardioprotection during ischemia,in neuroprotection during amyloid-related diseases, damage by alcohol or heavy metals, in obesitytreatment and in the treatment of diabetes-related disorders. Due to their structural analogy withclathrin-coated vesicles, fullerenes are also promising candidates for drug and gene delivery (for areview, see [12,13,18,119]).
Figure 1. Scheme of fullerene C60 (a) and of the preparation and structure of nanocellulose/fullerenecomposites (b).
However, fullerenes have low solubility in many solvents, especially in water. This is a majordrawback for their wider application in biomedical applications. The water solubility of fullerenescan be achieved by functionalizing them with hydrophilic groups, but this approach does not solveproblems arising from the aggregation and clustering of fullerenes. In addition, the formation ofsinglet oxygen, which is needed for photodynamic therapy, decreases after functionalization due tothe perturbation of the fullerene π system. These problems can be mitigated by the complexation offullerenes with water-soluble agents, including nanocellulose [35].
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2.2. Preparation and (Bio)Application of Nanocellulose/Fullerene Composites
Cellulose nanocrystals (CNCs) were used to create nanocellulose/fullerene composites. Thesenanocrystals are typically produced by acid hydrolysis of cellulose fibers, employing either sulfuric acidor hydrochloric acid in order to destroy the amorphous regions of the cellulose, while the crystallinesegments remain intact. CNCs can have a needle-like or rod-like morphology, and are also referred toas nanowhiskers or nanorods. This morphology is characterized by a high aspect ratio (i.e., high lengthto diameter ratio), and thus by a relatively large surface area. In addition, CNCs have a wide range ofother advantageous properties, such as high mechanical resistance, broad chemical-modifying capacity,renewability, biodegradability and low cytotoxicity [34,35]; for a review, see [2]. From these pointsof view, CNCs were considered ideal for immobilization of fullerene nanoparticles [92]. A schemeof preparation of nanocellulose/fullerene composites is depicted in Figure 1b. Composites of CNCswith fullerenes C60 were prepared by amine functionalization of CNCs and by subsequently graftingC60 onto the surface of amine-terminated CNCs [34]. Conversely, functionalized fullerenes, e.g.,polyhydroxylated fullerenes C60(OH)30, were conjugated with the surface of CNCs [92]. Both ofthese composites showed a higher radical scavenging capacity in vitro than fullerenes alone, andtherefore are promising for biomedical application in antioxidant therapies, e.g., as components ofskin care products. In the third type of composites, both cellulose nanocrystals and fullerenes werefunctionalized, i.e., amino-fullerene C60 derivatives were covalently grafted onto the surface of 2,2, 6, 6-tetramethylpiperidine-1-oxylradical (TEMPO)-oxidized nanocrystalline cellulose [35]. Thesecomposites hold promise for photodynamic cancer therapy (Table 1). When these composites wereadded to the culture medium of human breast cancer MCF-7 cells in the dark, they were taken upby these cells without changes in the cell viability, as revealed by a resazurin assay. However, whenirradiated with light, these composites showed dose-dependent toxicity for MCF-7 cells [35].
However, fullerenes are less widely used in nanocellulose/nanocarbon composites than othercarbon allotropes, particularly graphene and carbon nanotubes. More frequently, fullerenes areincorporated into a non-nanostructured cellulose matrix. For example, fullerene C70, characterizedby a strong thermally activated delayed fluorescence at elevated temperatures, which is extremelyoxygen sensitive, was incorporated into ethyl cellulose, i.e., a highly oxygen-permeable polymer. Thiscomposite was used for construction of an optical dual sensor for oxygen and temperature [115].An oxygen sensor was constructed using isotopically enriched carbon-13 fullerene C70, dissolved inan ethyl cellulose matrix [116]. Mixed-matrix membranes, consisting of ethyl cellulose as a continuousmatrix and fullerenes C60 as a dispersed phase, were prepared for propylene/propane separation [78].Electrospun cellulose acetate nanofibers reinforced with fullerenes were used in the construction ofdry-type actuators [123]. Cellulose impregnated with fullerenes C60 dissolved in o-xylene showedgreater extraction efficiency for Cu2+, Ni2+ and Cd2+ ions from an aqueous environment than thepure polymer [124]. Biocompatible composites containing polysaccharides (cellulose, chitosan andgamma-cyclodextrin) and fullerene derivatives (amino-C60 and hydroxy-C60) were developed forvarious applications ranging from dressing and treating chronically infected wounds to nonlinearoptics, biosensors, and therapeutic agents [118].
3. Nanocellulose/Graphene Composites
3.1. Characterization of Graphene
Graphene is a single layer of sp2-hybridized carbon atoms arranged into a two-dimensionalhoneycomb-like lattice (Figure 2a). In other words, graphene is a one-atom-thick layer of graphite.It is a basic building block for other carbon allotropes, such as fullerenes, carbon nanotubes andgraphite. Graphene is a very thin, nearly transparent sheet, but it is remarkably strong (about 100 timesstronger than steel), and highly electrically and thermally conductive (for a review, see [19,20,125,126]).Graphene can be prepared by various methods, which can be divided into two main categories, namelythe top-down approach and the bottom-up approach. The top-down approaches include treatment of
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graphite by mechanical or electrochemical exfoliation, intercalation or sonication, and also nanotubeslicing. The bottom-up approaches include growth of graphene from carbon-metal melts, epitaxialgrowth of graphene on silicon carbide, the dry ice method, and deposition methods such as chemicalvapor deposition or dip coating a substrate with graphene oxide (GO), followed by GO reduction (fora review, see [19,20,125,126]). Graphene can be prepared in the form of monolayer or bilayer sheets,nanoplatelets, nanoflakes, nanoribbons and nanoscrolls. Chemically, graphene-based materials includepure graphene sheets, GO or reduced graphene oxide (rGO). Pure graphene sheets can be producedby mechanical exfoliation of graphite or by chemical vapor deposition. GO, a highly oxidative andwater-soluble form of graphene, can be obtained by the exfoliation of graphite oxide. Reduced GO canbe prepared by chemical, thermal or pressure reduction, and even by bacteria-mediated reductionof GO, which improves its electrical properties (for a review, see [13,18,19,37,125,126]). Grapheneand graphene-based materials hold a great promise not only for a wide range of industrial andtechnology applications, but also for biomedical applications, such as drug, gene and protein delivery,photothermal therapy, construction of biosensors, bioimaging, antimicrobial treatment, and also asscaffolds for tissue engineering (for a review, see [20]).
Figure 2. Scheme of graphene (a) and of the preparation and structure of nanocellulose/graphenecomposites (b).
3.2. Preparation and Industrial Application of Nanocellulose/Graphene Composites
Similarly as in fullerenes, cellulose nanoparticles in the form of nanofibrils and nanocrystalsincrease the dispersion of graphene nanoparticles in water-based environments and prevent theiraggregation without the need to subject them to chemical functionalization [46]. A water-baseddispersion is the starting material for fabricating nanocellulose/graphene composites (Figure 2b). Thesecomposites can be created by filtration [127], filtration combined with hot pressing for fabricatingfilms [128], or by freeze-drying [75] and freeze-casting [48] for fabricating 3D materials, such as aerogelsand foams. Other methods are deposition of graphene on a nanocellulose layer [43] and incorporationof graphene into nanocellulose during its biological synthesis by bacteria [54–56].
All forms of nanocellulose and graphene have been used for constructing nanocellulose/graphenecomposites, i.e., CNFs, CNCs, unmodified graphene, GO and rGO. In order to modulate the propertiesof nanocellulose/graphene composites for specific applications, these materials can be further enrichedby various substances, such as metallic or ceramic nanoparticles, oxides, carbides, sulfides, vitaminC, synthetic and natural polymers, enzymes and antibodies. For example, nanocellulose/graphenecomposites have high adsorption, filtration and photocatalytic ability, and they are therefore widelyused for water purification, e.g., for removing antibiotics [75], dyes [43], heavy metals, such as Cu2+,Hg2+, Ni2+ and Ag+ [61,76], or for their bactericidal effect [56]. The water-cleaning capacity of thesecomposites can be further enhanced by introducing additional photocatalytic agents, i.e., palladium
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nanoparticles [54] or zinc oxide (ZnO) nanoparticles [73]. An optimized ultrafiltration membranefor water purification was constructed from polyvinylidene fluoride (PVDF), modified by cellulosenanocrystals functionalized with common bactericides, such as dodecyl dimethyl benzyl ammoniumchloride, ZnO and GO nanosheets [129]. Another important additive is vitamin C, which reducesthe GO in nanocellulose/GO composites, increases the surface area of the material and increases poreformation, and thus enhances the capacity of the composites for water purification [49]. A combinationof rGO-coated cellulose nanofibers with hydrophobic and oleophilic trimethyl chlorosilane enhancedthe adsorption capacity of this composite, which is necessary for effective removal of oil-basedpollutants from water [74].
Another important industrial application of nanocellulose/graphene composites is in energystorage, generation and conversion. Devices for these purposes include supercapacitors [64,80,130],hydrogen storage devices [131], electrodes for hydrogen evolution reaction [132], lithium ionbatteries [133], actuators [81], solar steam generators [82] and electric heating membranes [83].These devices can be based on pure nanocellulose/nanocarbon composites without additives [80–83].However, they often contain additives such as manganese oxide (MnO), which contributes tofaradaic pseudocapacitance in supercapacitors [130] or polypyrrole, which acts as an insulator,but its oxidized derivatives are good electrical conductors [134]. Other additives are palladium orplatinum nanoparticles for enhanced hydrogen storage [131], nitrogen-doped molybdenum carbidenanobelts in electrocatalysts for hydrogen evolution reaction [132], and silicon oxide nanoparticles inlithium ion batteries [133].
Other important industrial applications of nanocellulose/graphene composites are in theconstruction of fire retardants, shape memory devices, biocatalysts and materials for food packaging.Super-insulating, fire-retardant, mechanically strong anisotropic foams were produced by freeze-castingsuspensions of cellulose nanofibers, GO and sepiolite nanorods, and they performed better thantraditional polymer-based insulating materials [48]. Shape memory devices are based on GO/CNC thinfilms and nanomembranes [38,88] or on GO introduced into a nanocellulose paper made of nanofibersextracted from sisal fibers [89]. An example of a biocatalyst is a nanocellulose/polypyrrole/GOnanocomposite for immobilization of lipase, a versatile hydrolytic enzyme. This biocatalyst wasemployed for synthesizing ethyl acetoacetate, a fruit flavor compound [86]. Food packaging materialswere constructed by filling CNCs and rGO, either separately or in the form of CNC/rGO nanohybrids,into poly (lactic acid) (PLA) matrix or in a poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)matrix. These composite materials exhibited better mechanical properties than the pristine polymers,and possessed antibacterial activity. In addition, the composites with CNC/rGO nanohybrids performedbetter than those with a single component nanofiller, i.e., either CNCs or rGO. Due to their antibacterialactivity, antioxidant properties and good in vitro cytocompatibility, these composites are also promisingfor biomedical applications, e.g., as scaffolds for tissue engineering [67–69,87].
3.3. Biomedical Application of Nanocellulose/Graphene Composites
The biomedical applications of nanocellulose/nanographene composites include photothermalablation of pathogenic bacteria, combined chemo-photothermal therapy against cancer, drug delivery,biosensorics, isolation and separation of various biomolecules, wound dressing and particularly tissueengineering (Table 1).
For photothermal ablation of pathogenic bacteria, a composite paper containing nanocellulosewith Au linked to GO using quaternized carboxymethyl chitosan was developed. When excitedby near-infrared laser irradiation, the paper generated a rise in temperature of more than 80 ◦C,sufficient for photothermal ablation, both on Gram-positive bacteria (Bacillus subtilis and Staphylococcusaureus) and on Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Additionally, thecomposite paper showed a remarkable enhancement in tensile strength, bursting index and tear indexin comparison with the properties of pure nanocellulose paper [93].
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For chemophotothermal synergistic therapy of colon cancer cells, dual stimuli-responsivepolyelectrolyte nanoparticles were developed by layer-by-layer (LbL) assembly of aminatednanodextran and carboxylated nanocellulose on the surface of chemically modified GO. Tests on theHCT116 human colon cancer cell line revealed that these nanoparticles allowed for the intracellulardelivery of curcumin, which was released in response either to acidic environments or to near-infraredexcitation [94]. In this context, nanocellulose/graphene composites are good candidates as carriers forcontrolled drug delivery, particularly of anticancer drugs. Systems releasing doxorubicin, a model drugwith broad-spectrum anticancer properties, were developed. These systems included nanocompositecarboxymethyl cellulose/GO hydrogel beads [95], nanocomposite films made of graphene quantumdots incorporated into a carboxymethyl cellulose hydrogel [72] or macroporous polyacrylamidehydrogels. These hydrogels were prepared using an oil-in-water Pickering emulsion, containing GOand hydroxyethyl cellulose with a quaternary ammonium group [96].
Sensing and biosensing is another important application of nanocellulose/graphene composites.These sensors can be divided into electrochemical, piezoelectric, optical and acoustic wave-basedsensors. Electrochemical sensors were constructed for detecting cholesterol [98], glucose and pathogenicbacteria [110], avian leucosis virus [111] and organic liquids [112]. The biosensor for detecting cholesterolwas based on chemically-modified nanocellulose, grafted with silylated GO and enriched with ZnOnanoparticles in order to enhance its electrical conductivity [98]. The biosensor for detecting glucoseand pathogenic bacteria was based on nanocellulose paper coated with GO, reduced by vitamin C andfunctionalized with platinum nanoparticles with a cauliflower-like morphology in order to enhancethe electrical conductivity of the composite. The platinum surface was functionalized either withglucose oxidase (via chitosan encapsulation) or with an RNA aptamer [110]. The biosensor for theavian leucosis virus was an immunosensor, based on graphene-perylene-3, 4, 9, 10-tetracarboxylic acidnanocomposites as carriers for primary antibodies, on composites of nanocellulose and Au nanoparticlesas carriers for secondary antibodies, and on the alkaline phosphatase catalytic reaction [111]. The sensorfor organic liquids, mainly organic solvents, was based on cellulose nanocrystal-graphene nanohybrids,selectively located in the interstitial space between the natural rubber latex microspheres [112].
Piezoelectric nanocellulose/graphene-based sensors have usually been designed for strain sensing,i.e., as wearable electronics for monitoring the motion of various parts of the human body, e.g.,fingers [63,99,113]. For these purposes, the flexibility and stretchability of nanocellulose was furtherenhanced by adding other polymers, such as elastomers, represented e.g., by polydimethylsiloxane(PDMS) [113], or hydrogels, represented e.g., by poly(vinyl alcohol) (PVA), crosslinked (together withcellulose nanofibers and graphene) with borax [63].
Optical sensors can be based on surface-enhanced Raman spectroscopy (SERS) orfluorescence. Cellulose SERS strips decorated with plasmonic nanoparticles, termedgraphene-isolated-Au-nanocrystals (GIANs), were developed for constructing portable sensors fordetecting complex biological samples, e.g., for detecting free bilirubin in the blood of newborns [100].A fluorescence sensor, based on sulfur and nitrogen-co-doped graphene quantum dots, immersedinto nanocellulosic hydrogels, was developed for detecting laccase. This enzyme is widely used inindustrial and technological applications, such as bleaching of fabrics, tooth whitening, decoloration ofhair, water purification and in oxidizing dyes in beer, must and wines [71].
An example of an acoustic wave-based sensor is an ammonia sensor, based on a quartz crystalmicrobalance (QCM) with a sensing coating. This coating is composed of negatively-chargedelectrospun cellulose acetate nanofibers, positively-charged polyethylenimine and negatively-chargedGO, and it was created by the electrostatic LbL self-assembly technique [101].
For protein isolation, a metal affinity carboxymethyl cellulose-functionalized magneticgraphene was prepared by successive modifications of GO nanosheets with magnetic nanoparticles,carboxymethyl cellulose and iminodiacetic acid, and then chelated with copper ions. This compositeexhibited high adsorption selectivity toward histidine-rich proteins, which was utilized for isolatinghemoglobin from human whole blood, and also for isolating a polyhistidine-tagged recombinant
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protein from Escherichia coli lysate, namely Staphylococcus aureus enterotoxin B [77]. For macromolecularseparation, cellulose acetate nanocomposite ultrafiltration membranes were fabricated using 2D layerednanosheets, e.g., GO and exfoliated molybdenum disulfide (MoS2), and were successfully tested usingmacromolecular bovine serum albumin [79].
Nanocellulose/graphene composites are also important components of tissue engineering scaffolds,improving their mechanical properties and their bioactivity. In the studies by Pal et al. (2017), mentionedabove, a PLA/CNC/rGO nanocomposite film showed antibacterial activity against Gram-positiveStaphylococcus aureus and against Gram-negative Escherichia coli. At the same time, this film exhibitednegligible cytotoxicity against a mouse NIH-3T3 fibroblast cell line, as revealed by an MTT assayof the activity of mitochondrial oxidoreductase enzymes [87]. Nanocomposites of CNCs and rGO,incorporated into PLA matrix through the melt-mixing method, were noncytotoxic and cytocompatiblewith epithelial human embryonic kidney 293 (HEK293) cells [68]. PLA incorporated with rGO andTEMPO-oxidized CNCs, grafted with poly(ethylene glycol) (PEG), displayed radical scavenging activityand negligible toxicity and cytocompatibility to mouse embryonic C3H10T1/2 cells [69]. A compositefilm consisting of hydrophilic bacterial cellulose nanofibers and hydrophobic rGO, prepared from GOusing a bacterial reduction method, supported the adhesion, viability and proliferation of human bonemarrow mesenchymal stem cells in a similar way to standard cell culture polystyrene, and better thanpure rGO films [37]. Incorporating GO into electrospun cellulose acetate nanofibrous scaffolds enhancedthe adhesion and growth of human umbilical cord mesenchymal stem cells. It also enhanced osteogenicdifferentiation of these cells, manifested by the activity of alkaline phosphatase, and biomineralizationof the scaffolds in a simulated body fluid [59]. Nanofibrous composites of bacterial nanocellulose,a conductive poly(3,4-ethylene dioxythiophene) (PEDOT) polymer and GO, mimicking the nativeextracellular matrix and allowing electrical stimulation of neural PC12 cells, induced specific orientationand differentiation of these cells [105]. Polyvinyl alcohol/carboxymethyl cellulose (PVA/CMC) scaffoldsloaded with rGO nanoparticles, prepared by lyophilization, enhanced the proliferation of EA.hy926endothelial cells in vitro and angiogenesis in vivo using a chick chorioallantoic membrane model [107].Polyacrylamide-sodium carboxymethylcellulose hybrid hydrogels reinforced with GO and/or CNCsalso have potential for tissue engineering applications due to their tunable mechanical properties [135].Genetically modified hydrophobin, a fungal cysteine-rich protein, was used to connect nanofibrillatedcellulose of wood origin and graphene flakes in order to construct biomimetic mechanically-resistantmaterials similar to nacre and combining high toughness, strength and stiffness [136].
Nanocellulose/graphene composites also have great potential for the fabrication of antibacterialtextiles and for advanced wound dressing. Antibacterial textiles were prepared by electrospinning amixture containing cellulose acetate, TiO2 and GO sheets. These textiles showed high antibacterialactivity with an inhibition rate higher than 95% against Bacillus subtilis and Bacillus cereus [137]. Bacterialcellulose is considered as one of the most suitable materials for advanced wound dressing, due to itsappropriate mechanical properties, such as strength, Young’s modulus, elasticity and conformability,and also due to its great capacity to retain moisture in the wound (for a review, see [2]). These favorableproperties can be further enhanced by adding graphene-based materials and by crosslinking withsynthetic polymers, such as poly (ethylene glycol), poly (vinyl alcohol), poly (acrylic acid) and poly(acrylamide). In a study by Chen et al. (2019), a bacterial nanocellulose-grafted poly (acrylic acid)/GOcomposite hydrogel was prepared as a potential wound dressing. The inclusion of GO improvedthe attachment and proliferation of human dermal fibroblasts in cultures on the composites [108].Similarly, hydroxypropyl cellulose matrix incorporated with GO and silver-coated ZnO nanoparticlesshowed improved tensile strength, and also anti-ultraviolet, antibacterial and immunostimulatoryeffects, which promoted wound healing in an in vivo mouse model [109].
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4. Nanocellulose/Carbon Nanotube Composites
4.1. Characterization of Carbon Nanotubes
Carbon nanotubes (CNTs; Figure 3a) are tubular structures formed by a single cylindrical graphenesheet (single-walled carbon nanotubes, referred to as SWCNTs or SWNTs) or several graphene sheetsarranged concentrically (multiwalled carbon nanotubes, referred to as MWCNTs or MWNTs, whichalso include double-walled CNTs (DWCNTs [16]), and few-walled CNTs (FWCNTs [17]). Carbonnanotubes were discovered as a by-product of fullerene synthesis, and were first described by Iijima etal. (1991) [138]. CNTs have a high aspect ratio (i.e., length to diameter ratio) and thus a relatively largesurface area. Their diameter is on the nanometer scale (e.g., from 0.4 nm to 2–3 nm in single-wallednanotubes), but their length can reach several micrometers or even centimeters. Due to these properties,CNTs are suitable candidates for hydrogen storage, for the removal of contaminants from water andair, and also for drug delivery. CNTs have excellent mechanical properties, mainly due to the sp2
bonds. The tensile strength of SWCNTs has been reported to be almost 100 times higher than that ofsteel, while their specific weight is about six times lower. CNTs can therefore be used for reinforcingvarious synthetic and natural polymers for industrial and biomedical applications, e.g., for hard tissueengineering. When added to a polymer matrix, CNTs can resemble inorganic mineral nanoparticlesin the bone tissue, and they can form nanoscale irregularities on the surface of 2D materials andin the pores of 3D materials, which improve the cell adhesion and growth. CNTs are electricallyconductive and enable electrical stimulation of cells, which further improves the adhesion, growth anddifferentiation of cells (for a review, see [10–14,18,139]). However, free CNTs can be cytotoxic, which isattributed to their ability to cause oxidative damage, and also to their contamination with transitionmetals (e.g., Fe, Ni, Y), which serve as catalysts during CNT preparation. Methods for producingmetal-free CNTs have therefore been developed, e.g., arc-discharge evaporation of graphite rods [139].
Figure 3. Scheme of multi-walled and single-walled carbon nanotubes (a) and of the preparation andstructure of nanocellulose/carbon nanotube composites (b).
CNTs also resemble CNFs from the point of view of their morphology and their mechanicalproperties. For example, highly crystalline, thick CNFs derived from tunicates exhibited mean strengthof 3–6 GPa, which was comparable with commercially available MWCNTs. However, the mean strengthof other types of CNFs is lower; for example, in wood-derived CNFs the mean strength ranged from1.6 to 3 GPa [140]. CNTs therefore improve the mechanical strength of nanocellulose/CNT composites,and endow them with electrical conductivity, similarly as graphene. As a result, nanocellulose/CNTcomposites are used in similar industrial and biomedical applications as nanocellulose/graphenecomposites, e.g., water purification, energy generation, storage and conversion, filling polymericmaterials, constructing sensors and biosensors, drug delivery, cancer treatment, electrical stimulationof tissues, and tissue engineering.
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4.2. Preparation and Industrial Application of Nanocellulose/CNT Composites
The nanocellulose component in the nanocellulose/CNT composites is used in the form ofnanofibrils and nanocrystals, and carbon nanotubes usually in the form of SWCNTs or MWCNTs.Similarly as in composites containing graphene and other carbon allotropes, cellulose nanoparticlesfacilitate the homogeneous dispersion of CNTs in aqueous environments (Figure 3b), where the twotypes of nanoparticles are linked by noncovalent interactions, e.g., hydrophobic and electrostaticinteractions [42,46]. The dispersion of CNTs can be further facilitated by TEMPO-mediated oxidationof cellulose nanoparticles, which endows them with abundant anionic carboxyl groups [141].Other ways include the functionalization of CNTs with self-assembling amphiphilic glycosylatedproteins [142] or the use of oil-in-water Pickering emulsions of cellulose nanoparticles [143]. Fromthe aqueous dispersions, 2D and 3D nanocellulose/CNT composites can be formed, e.g., by vacuumfiltration, centrifugal cast molding, foam forming, casting and printing [39,45,50,141]. Pickeringemulsions of cellulose nanocrystals and SWCNTs or MWCNTs were used for fabricating aerogelsand foams by freeze-drying [143]. Similarly as in nanocellulose/graphene composites, CNTs canbe added to cultures of bacteria producing nanocellulose, and incorporated into the bacterialnanocellulose during its growth [53,57]. Composite nanocellulose/SWCNT films can be transparent orsemitransparent [39,45,141], and can transmit radiant energy [144].
Like nanocellulose/graphene composites, nanocellulose/CNT composites can be combined withvarious atoms, molecules and nanoparticles in order to enhance their properties for specific applications.For example, Ag nanoparticles attached to the surface of MWCNTs influenced the electrochemicalproperties of CNT-based films developed on a bacterial nanocellulose membrane [36]. Nanocelluloseand CNTs can be also used as additives to various hybrid materials. For example, a hybrid material,created by combination of poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS),silver nanoparticles (AgNPs), CNTs and a nanocellulose layer, was used for constructing a tactilesensor [103]. Incorporation of polypyrrole-coated CNTs into chemically cross-linked CNC aerogelscreated promising materials for flexible 3D supercapacitors [145]. A combination of cellulose acetate,chitosan and SWCNTs with Fe3O4 and TiO2 in electrospun nanofibers enables combined removal ofCr6+, As5+, methylene blue and Congo red from aqueous solutions via the adsorption and photocatalyticreduction processes [146].
Energy-related applications of nanocellulose/CNT composites include biofuel cells, varactors,supercapacitors, and electrodes for lithium batteries, thermoelectric generators for heat-to-electricityconversion or for constructing heating elements [50]. A biofuel cell comprising electrodes based onsupercapacitive materials, i.e., on CNTs and a nanocellulose/polypyrrole composite, was utilizedto power an oxygen biosensor. Laccase, immobilized on naphthylated MWCNTs, and fructosedehydrogenase, adsorbed on a porous polypyrrole matrix, were used as cathode and anodebioelectrocatalysts, respectively [84]. Another biofuel cell was based on a conductive MWCNTnetwork, developed on a bacterial nanocellulose film, and functionalized with redox enzymes,including pyroquinoline quinone glucose dehydrogenase (anodic catalyst) and bilirubin oxidase(cathodic catalyst). This system generated electrical power via the oxidation of glucose and thereduction of molecular oxygen [44]. Microelectromechanical system varactors, i.e., voltage-controlledcapacitors, consisted of a freestanding SWCNT film, which was employed as a movable component,and a flexible nanocellulose aerogel filling [85]. Supercapacitors with high physical flexibility,desirable electrochemical properties and excellent mechanical integrity were realized by rationallyexploiting the unique properties of bacterial nanocellulose, CNTs, and ionic liquid-based polymergel electrolytes [147]. Other flexible 3D supercapacitor devices were fabricated by incorporatingpolypyrrole nanofibers, polypyrrole-coated CNTs, and manganese dioxide (MnO2) nanoparticlesin chemically cross-linked cellulose nanocrystal aerogels [145]. Electrospun core-shell nanofibrousmembranes, containing CNTs stabilized with cellulose nanocrystals, were developed for use ashigh-performance flexible supercapacitor electrodes with enhanced water resistance, thermal stabilityand mechanical toughness [40]. Electrodes for lithium batteries were based on freestanding
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LiCoO2/MWCNT/cellulose nanofibril composites, fabricated by a vacuum filtration technique [148],or on freestanding CNT-nanocrystalline cellulose composite films [41]. Thermoelectric generatorsfor heat-to-electricity conversion were based on large-area bacterial nanocellulose films with anembedded/dispersed CNT percolation network, incorporated into the films during nanocelluloseproduction by bacteria in culture [57]. Electrical energy can also be converted into thermal energy,as demonstrated by the composite structure of wood-derived nanocellulose, MWCNTs and pulp,designed for a heating element application [50].
4.3. Biomedical Application of Nanocellulose/CNT Composites
Biomedical applications of composites of cellulose and CNTs are summarized in Table 1. Thesecomposites are important systems for drug delivery. The CNTs in the composites can be conjugated withmany therapeutics, usually anticancer drugs, but also other types of drugs. For example, an osmoticpump tablet system coated with cellulose acetate membrane containing MWCNTs was developed fordelivery of indomethacin (for a review, see [16,65]). Cancer cells can be killed by nanocellulose-CNTdispersions even without the presence of anticancer drugs. For example, nonmercerized type-IIcellulose nanocrystals in dispersions with SWCNTs displayed cytotoxicity for human epithelialcolorectal adenocarcinoma Caco-2 cells, but they enhanced the mitochondrial metabolism of normalcells [149].
Construction of sensors and biosensors is another important application of nanocellulose/CNTcomposites useful for (bio) technology and medicine. A tactile piezoresistance and thermoelectric-basedsensor, mentioned above, which is capable of simultaneously sensing temperature and pressure, isfabricated from TEMPO-oxidized cellulose, PEDOT:PSS, AgNPs and CNTs [103]. Another pressuresensor was developed using aerogels consisting of plant cellulose nanofibers and functionalizedfew-walled CNTs [17]. Highly conductive and flexible membranes with a semi-interpenetratingnetwork structure, fabricated from MWCNTs and cellulose nanofibers, showed the electrical featuresof capacitive pressure sensors and were promising for various electronics applications, e.g., touchscreens [150]. Advanced flexible strain sensors for controlling the human body motion were fabricatedby pumping hybrid fillers consisting of CNTs/CNCs into porous electrospun thermoplastic polyurethanemembranes [91]. Other strain sensors were fabricated by a facile latex assembly approach, in whichCNCs played a key role in tailoring the percolating network of conductive natural rubber/CNTcomposites [114]. A water-responsive shape memory hybrid polymer, based on a thermoplasticpolyurethane matrix crosslinked with hydroxyethyl cotton cellulose nanofibers and MWCNTs, was alsodeveloped for constructing a strain sensor [90]. A flexible and highly sensitive humidity sensor, capableof monitoring human breath, was based on TEMPO-oxidized nanofibrillated cellulose and CNTs [104].An electrochemical biosensor for three adenosine triphosphate (ATP) metabolites, namely uric acid,xanthine and hypoxanthine, was based on a composite of NH2-MWCNT/black phosphorene/AgNPs,dispersed in carboxymethyl cellulose [102]. Another electrochemical molecularly-imprinted sensor wasbased on a nanofibrous membrane prepared by the electrospinning technique from cellulose acetate,MWCNTs and polyvinylpyrrolidone, and was used for determining ascorbic acid [151]. An oxygenbiosensor powered by a biofuel cell containing MWNCTs, a nanocellulose/polypyrrole composite,laccase and fructose dehydrogenase, was mentioned above [84]. Versatile wearable textile sensors, e.g.,for gas sensing, were produced from cellulose nanofibers extracted from tunicates, homogeneouslycomposited with SWCNTs, by wet spinning in an aligned direction [60].
Other important biomedical applications of nanocellulose/CNT composites are in electricalstimulation of cells and tissues in order to improve their regeneration and function, and in tissueengineering. For example, stretchable, flexible and electrically conductive biopatches for restoratingconduction in damaged cardiac regions and for preventing arrhythmias were prepared. Thesepatches were based on nanofibrillated cellulose/SWCNT ink three-dimensionally printed onto bacterialnanocellulose. They restored cardiac conduction after its disruption by a surgical incision made inthe ventricular part of the heart in experimental dogs [52]. For neural tissue stimulation, multiblock
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conductive nerve scaffolds with self-powered electrical stimulation were prepared. These scaffoldswere based on polypyrrole/bacterial nanocellulose composites with platinum nanoparticles on theanode side for glucose oxidation, and nitrogen-doped CNTs on the cathode side for oxygen reduction.These scaffolds enhanced the elongation of neurites outgrowing from rat dorsal root ganglions in vitroand stimulated nerve regeneration in a rat sciatic nerve gap model in vivo in comparison withcomposites containing only polypyrrole and bacterial nanocellulose. These scaffolds could replacethe metal needles that are currently used for external electrical stimulation of neural tissue, whichmay cause pain and a risk of infection [106]. 3D printing was also used for creating scaffolds basedon a conductive ink composed of wood-derived CNFs and SWCNTs. These scaffolds were intendedfor neural tissue engineering for experimental brain studies, and supported the attachment, growthand viability of human neuroblastoma SH-SHY5Y cells [51]. Other scaffolds for tissue engineeringconsisted of electrospun cellulose acetate nanofibers, assembled with positively-charged chitosanand negatively-charged MWCNTs via an LbL technique. These scaffolds promoted the adsorptionof cell adhesion-mediating molecules from the serum supplement of the culture medium and theadhesion and growth of mouse subcutaneous L929 fibroblasts [117]. Our own results related to thepotential application of nanocellulose/CNT composites as scaffolds for tissue engineering are reportedin Appendix A.
5. Nanocellulose/Nanodiamond Composites
5.1. Characterization of Nanodiamond
Diamond is an allotrope of carbon, consisting of carbon atoms arranged in a cubic crystal structurecovalently bonded in sp3 hybridization (Figure 4a). Like all nanostructured materials, nanodiamondsor diamond nanoparticles are defined as features not exceeding 100 nm in at least one dimension,although some larger diamond particles, i.e., 125–210 nm, are still referred to as nanodiamonds (fora review, see [152]). At the same time, the size of ultrananocrystalline diamond particles is 3–5nm [153,154]. Diamond nanoparticles can be prepared by various methods. The most widely usedtechniques are detonation of carbon-containing explosives in an oxygen-deficit environment andmicrowave-enhanced plasma chemical vapor deposition (MECVD). Other techniques include theradiofrequency plasma-assisted chemical vapor deposition (PACVD) method, milling of diamondmicrocrystals, hydrothermal synthesis, ion bombardment, laser bombardment, ultrasound synthesisand electrochemical synthesis (for a review, see [10,152–156]). Nanodiamonds are considered to bethe most advanced carbon materials in the world. This is due to their excellent mechanical, optical,electrical, thermal and chemical properties. The mechanical properties of nanodiamonds includethe highest hardness of all materials on earth, a high Young’s modulus, high fracture toughness,high pressure resistance and a low friction coefficient. Their optical properties include transparency,high optical dispersion, and their ability to display various colors and to emit intrinsic luminescence(fluorescence), which is due to defects in the diamond lattice or contamination of the lattice withforeign atoms, such as N, B, H, Ni, Co, Cr or Si. Regarding their electrical properties, nanodiamondscan act as good insulators in their pristine state and as semiconductors after doping, usually withboron. Their thermal properties include superior thermal conductivity and low thermal expansion.The chemical properties of nanodiamonds include low chemical reactivity and resistance to liquid- andgas-phase oxidations. However, nanodiamonds can be doped with various atoms, and their surfacecan be functionalized by various atoms, chemical groups and (bio)molecules ([152,153,157,158]; for areview, see [10–15,155]).
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Figure 4. Scheme of a nanodiamond (a) and of the preparation and structure ofnanocellulose/nanodiamond composites (b).
5.2. Preparation and (Bio)Application of Nanocellulose/Nanodiamond Composites
There is greater use of diamond nanoparticles than of fullerenes in nanocellulose/nanocarboncomposites, but diamond nanoparticles are used less than graphene and carbon nanotubes. This maybe because a nanodiamond is more expensive and is electrically nonconductive in its pristine state. Incomposites with nanofibrillated cellulose (Figure 4b), a nanodiamond was used for constructing highlythermally conductive, mechanically resistant and optically transparent films with potential applicationas lateral heat spreaders for portable electronic equipment [70]. A highly mechanically resistant andoptically transparent nanopaper was made of cationic CNFs and anionic nanodiamond particles byfiltration from a hydrocolloid and subsequent drying [159]. Moreover, a diamond can be renderedelectrically semiconductive by doping it with boron, and then can be used for constructing biosensors.For example, a sensor for biotin was developed by the adsorption of captavidin, a nitrated avidin withmoderate affinity to biotin, on a carboxymethylcellulose layer stabilized on a boron-doped diamondelectrode by a Nafion film. This biosensor was used for analyzing biotin in blood plasma [66] (Table 1).
The reinforcing effect of diamond nanoparticles, coupled with their optical transparency,has also been used advantageously for other biomedical applications, particularly for wounddressing. Nanocellulose/nanodiamond composites are more mechanically resistant than purelynanocellulose-based materials, but they retain their flexibility and stretchability. In addition, theiroptical transparency enables direct inspection of wounds without the need to remove the dressing.For example, incorporating diamond nanoparticles in a concentration of 2 wt % into chitosan/bacterialnanocellulose composite films resulted in a 3.5-fold increase in the elastic modulus of these films. Thesecomposite films were transparent, but their transparency can be modulated by the concentration ofdiamond nanoparticles, turning them gray and semitransparent at higher nanodiamond concentrations(3 and 4 wt %). The viability of mouse subcutaneous L929 fibroblasts in cultures on these films,evaluated by an MTT test of the activity of cell mitochondrial enzymes, was more than 90% at 24 h afterseeding. However, at 48 h, it had dropped to about 75%, which indicated that diamond nanoparticlesare slightly cytotoxic [62]. A similar result was obtained on L929 fibroblasts grown on electrospuncomposite nanofibrous mats containing chitosan, bacterial cellulose and 1–3 wt % medical-gradenanodiamonds [58]. The viability of these cells, estimated by the MTT assay, dropped from approx.90% on day 1 to approx. 75% on day 3. Nevertheless, the addition of nanodiamonds facilitated theelectrospinning process, reduced the diameter of the nanofibers in the mats, regulated the water vaporpermeability of the mats, enhanced their hydrophilicity and improved their mechanical properties to asimilar level as in native skin [58].
Adding diamond nanoparticles per se did not significantly increase the antibacterial activityof chitosan/bacterial nanocellulose composites [62]. This activity can be further enhanced, e.g., by
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adding silver nanoparticles [160]. Nanocellulose/nanodiamond composites can also act as a suitableplatform for drug delivery. This was demonstrated on transparent doxorubicin-loaded carboxylatednanodiamonds/cellulose nanocomposite membranes, which are promising candidates for wounddressings. These membranes are porous, transparent, with appropriate mechanical properties, andwithout doxorubicin they are noncytotoxic for HeLa cells [97].
6. Composites of Nanocellulose with Other Carbon Nanoparticles
In addition to fullerenes, graphene, nanotubes and nanodiamonds, other important carbonnanoparticles used in industrial, biotechnological and biomedical applications include carbonnanofibers, carbon quantum dots and nanostructures formed by activated carbon and carbon black.All these nanomaterials can be used in nanocellulose/nanocarbon composites. The biomedicalapplications of these composites are summarized in Table 1.
6.1. Composites of Nanocellulose and Carbon Nanofibers
Carbon nanofibers can be created by carbonization of cellulose nanofibers originating from bacterialnanocellulose [21,23,161], urea [161], filter paper [162] or plant-derived cellulose [22,24]. Anothermethod of preparing carbon nanofibers is chemical vapor deposition (CVD; [25]). These carbonnanofibers can be further combined with other carbon nanoparticles, mainly graphene. For example,a composite paper consisting of nitrogen-doped carbon nanofibers, reduced graphene oxide (rGO)and bacterial cellulose was designed as a high-performance, mechanically tough, and bendableelectrode for a supercapacitor. The bacterial nanocellulose in this paper is exploited both as a biomassprecursor for the creation of carbon nanofibers by pyrolysis and as a supporting substrate for thenewly-created material [21]. In another study, highly conductive freestanding cross-linked carbonnanofibers, derived from bacterial cellulose in a rapid plasma pyrolysis process, were used as substratesfor the growth of vertically-oriented graphene sheets for constructing alternating current filteringsupercapacitors [23]. A small amount of rGO can also act as an effective initiator of carbonizationof cellulose nanofibers through microwave treatment [24]. Carbonization of aerogels, preparedfrom a mixture of PVA, cellulose nanofibers and GO by freeze-drying, enhanced the hydrophobicproperties, the specific surface area and the adsorption capacity of these aerogels. These materials thenbecame suitable candidates for oil-water separation and environmental protection [22]. In additionto graphene, cellulose-derived carbon nanofibers can be combined with various other nanoparticlesand nanostructures, such as Pt nanoparticles for methanol oxidation reaction [161], TiO2 films andFe3O4 nanoparticles for lithium ion batteries [162], tin oxide (SnO) nanoparticles for lithium-sulfurbatteries [163] or NiCo2S4 nanoparticles for hydrogen evolution reaction [164]. Carbon nanofibersare also promising for biomedical applications, particularly bone tissue engineering. Their nanoscalediameter produced a nanoscale surface roughness of their compacts or of their composite withpoly-lactic-co-glycolic acid (PLGA). This nanoroughness promoted preferential adhesion of osteoblastsfrom other cell types, particularly fibroblasts, which could prevent fibrous encapsulation of boneimplants [25].
6.2. Composites of Nanocellulose and Carbon Quantum Dots
Carbon quantum dots (CQDs) are quasispherical carbon nanoparticles (less than 10 nm indiameter) with a chemical structure and physical properties similar to those of graphene oxide. Thesenanoparticles emit a strong wavelength-dependent fluorescence. By changing the CQD size, the colorof the emitted light can be tuned from deep ultraviolet to visible and near-infrared light. In addition, thefluorescence of CQDs, and also their water solubility, can be further modulated by functionalizing theirsurface with various atoms, chemical functional groups and molecules, such as metals, carboxyl groups,organic dyes and polymers. CQDs present good photostability, low photobleaching and relativelylow cytotoxicity, and they are therefore considered to be suitable for biomedical applications such asbioimaging, biosensing, photodynamic and photothermal therapy of cancer, and drug delivery [165].
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In hybrid materials with nanocellulose, CQDs were applied for constructing biosensors anddrug delivery systems, and also for water purification. An optical sensor for visual discriminationof biothiols was based on a bacterial cellulose nanopaper substrate with ratiometric fluorescentsensing elements. These elements included N-acetyl l-cysteine capped green cadmium telluride (CdTe)quantum dots-rhodamine B and red CdTe quantum dots-carbon dots [26]. Hybrid materials containingcarbon quantum dots and cellulose are also promising carriers for drug delivery. Composite core/shellchitosan-poly (ethylene oxide)-carbon quantum dots/carboxymethyl cellulose-poly(vinyl alcohol)nanofibers were prepared through coaxial electrospinning as a biodegradable implant for local deliveryof temozolomide (TMZ), an anticancer drug. When tested in vitro, the antitumor activity of TMZconjugated with carbon quantum dots against the tumor U251 cell lines was higher than the activity ofthe free drug [28]. Last but not least, carbon quantum dots, homogeneously dispersed together withmagnetic Fe3O4 nanoparticles in electrospun cellulose nanofibers, were promising for the removal ofHg(II) ions from water [27].
6.3. Composites of Nanocellulose and Activated Carbon
Activated carbon is a form of carbon processed to have small, low-volume pores that increase thesurface area, which is then available for the adsorption and removal of various toxic contaminantsand microorganisms. Composite membranes consisting of a bilayer of porous activated carbonand TEMPO-oxidized plant-derived CNFs showed high capability for removing Escherichia colifrom water [29]. Activated carbon was also a component of a wound dressing material consistingof a polyvinyl alcohol and cellulose acetate phthalate polymeric composite film, reinforced withCu/Zn bimetal-dispersed activated carbon micro/nanofibers. This material suppressed the growth ofPseudomonas aeruginosa, the most prevalent bacteria in infected wounds caused by burns, surgery andtraumatic injuries [30].
6.4. Composites of Nanocellulose and Carbon Black
Carbon black is a form of paracrystalline carbon, produced industrially by partial combustion orthermal decomposition of gaseous or liquid hydrocarbons under controlled conditions. Carbon blackhas a high surface-area-to-volume ratio, though not so high as that of activated carbon. Although itis considered to have low toxicity, the International Agency for Research on Cancer has classified itas possibly carcinogenic to humans. In addition, as a component of environmental pollution, carbonblack can cause oxidative damage and an inflammatory reaction, which further mediate genotoxicity,reproductive toxicity, neurotoxicity and diseases of the respiratory and cardiovascular systems [166,167].Nevertheless, carbon black is currently used as a filler in tires and in other rubber products, and as apigment in inks, paints and plastics.
Composites of nanocellulose and carbon black have been used mainly for constructing biosensors,particularly wearable sensors for strain and human body motion, e.g., motion of the fingers, the elbowjoint and the throat. A strain-sensing device with excellent waterproof, self-cleaning and anticorrosionproperties was based on a superhydrophobic electrically conductive paper. This paper fabricatedby dip-coating a printing paper into a carbon black/carbon nanotube/methyl cellulose suspensionand into a hydrophobic fumed silica suspension [33]. Another strain-sensing device was fabricatedby printing carbon black conductive nanostructures on cellulose acetate paper. At the same time,this material had electrochemical properties promising for the detection of hydrogen peroxide [31].An electrochemical aptasensor for detecting Staphylococcus aureus, e.g., in human blood serum, wasdesigned as a nanocomposite of Au nanoparticles, carbon black nanoparticles and cellulose nanofibers,and was endowed with a thiolated specific S. aureus aptamer as a sensing element [32].
7. Potential Cytotoxicity and Immunogenicity of Nanocellulose/Nanocarbon Composites
The vast majority of studies dealing with potential biomedical applications ofnanocellulose/nanocarbon composites have reported no cytotoxicity or negligible cytotoxicity
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of these composites, namely of nanocellulose/fullerene composites [35], nanocellulose/graphenecomposites [68,69,72,87,107], nanocellulose/CNT composites [51,149] and nanocellulose/nanodiamondcomposites [58,62,97]. Composites containing other carbon nanoparticles, such as activated carbonnanoparticles [30] or carbon quantum dots [28] have also shown no significant cytotoxic effects. In thementioned studies, cytotoxicity was mainly tested in vitro on various cell types, such as fibroblasts,epithelial and endothelial cells, and mainly on cell lines, including tumor cell lines. The cell viabilityand proliferation were usually evaluated by tests of the activity of mitochondrial enzymes, such asMTT assay, resazurin (Alamar Blue) assay, or by a direct microscopic examination of the cells. Somecomposites have also been tested in vivo, e.g., in a rat model (nanocellulose/CNT composites; [106]), acanine model (nanocellulose/CNT composites; [52]), or using a chick chorioallantoic membrane model(nanocellulose/graphene composites, [107]), without adverse effects.
However, the individual components of nanocellulose/nanocarbon composites, particularly carbonnanoparticles, can act as cytotoxic, if they are not bound to any matrix and are free to move. Grapheneand graphene-based carbon nanomaterials, such as fullerenes and nanotubes, are hydrophobic intheir pristine state, and can enter into hydrophobic interactions with cholesterol in the cell membrane,which can be extracted from the membrane. In this manner, carbon nanoparticles can damage cellseven without penetrating them. Another mechanism of cell membrane damage is the generation ofreactive oxygen species (ROS) by carbon nanoparticles. In addition, the nanoparticles can penetrate thecell membrane, and can cause oxidative damage to mitochondria, and can also enter the cell nucleusand act as genotoxic agents (for a review, see [10,12,19,20]). Nanodiamonds have been consideredto be relatively nontoxic in comparison with other carbon nanoparticles. However, as shown in ourearlier studies, hydrophobic, hydrogen-terminated and positively-charged diamond nanoparticlescan enter the cells, impair their growth and cause cell death [152,156]. The mechanism of cell damageby nanodiamonds is by generating ROS, and by excessive delivery of sodium ions adsorbed on thenanodiamond surface [168]. Last but not least, carbon nanoparticles can be immunogenic, i.e., theycan activate inflammatory reactions, which can be, as has been demonstrated on carbon black, themain pathogenic mechanism of respiratory, cardiovascular and other serious diseases [166,167].
Cellulose nanoparticles, which are generally considered to be biocompatible [34,114] and of alow ecological toxicity [169], can also act as cytotoxic and immunogenic. It has even been speculatedthat, due to their high aspect ratio and stiffness, CNCs may cause similar pulmonary toxicity ascarbon nanotubes and asbestos [170]. In a mouse model, cellulose nanocrystals induced oxidativestress, caused pulmonary inflammation and damage, increased levels of collagen and transforminggrowth factor-beta (TGF-β) in lungs, and impaired pulmonary functions [170]. In addition, theseeffects were markedly more pronounced in female mice than in male mice. The immunogenicity ofCNCs was also proven in vitro. CNCs and their cationic derivatives CNC-aminoethylmethacrylate andCNC-aminoethylmethacrylamide evoked an inflammatory response in mouse macrophage J774A.1cells and in peripheral blood mononuclear cells by increasing the level of ROS in mitochondria, therelease of ATP from mitochondria and by stimulating the secretion of interleukin-1beta (IL-1β) [171].The cytotoxicity and immunogenicity of CNCs depend on the preparation conditions and areincreased under harsh and caustic conditions, e.g., the so-called mercerization process, i.e., analkali treatment [149]. CNFs can also cause cytotoxicity and oxidative damage, which can be evenmore pronounced than in the case of CNCs, and can evoke an inflammatory response (for a review,see [2,172]). The potential cytotoxicity and immunogenicity of nanocellulose, nanocarbon and theircomposites should therefore be taken into account when they are for use in biomedical applications.
8. Conclusions
Nanocellulose/nanocarbon composites and other hybrid materials containing cellulosenanoparticles (nanofibrils or nanocrystals) and carbon nanoparticles (fullerenes, graphene, carbonnanotubes, nanodiamonds and other carbon nanoparticles) are novel materials that are promising fora wide range of applications in industry, (bio)technology and medicine. This is due to their unique
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properties, such as high mechanical strength coupled with flexibility and stretchability (compositeswith graphene, carbon nanotubes and nanodiamond), shape memory (composites with grapheneand carbon nanotubes), photodynamic and photothermal activity (composites with fullerenes andgraphene), electrical conductivity (composites with graphene and carbon nanotubes), semiconductivity(composites with boron-doped diamond), thermal conductivity (composites with graphene andnanodiamonds), tunable optical transparency (composites with single-walled carbon nanotubes andnanodiamonds), intrinsic fluorescence and luminescence (composites with graphene quantum dotsand carbon quantum dots), and high adsorption and filtration capacity (composites with graphene,carbon nanotubes and carbon quantum dots). These properties arise mainly from the advantageouscombination of nanocellulose and nanocarbon, which associates and enhances the desirable effectsof each of these components. These materials can be prepared relatively easily from a water-basedsuspension, which is advantageous particularly for biomedical applications. These applications includedrug delivery, biosensorics, isolation of various biomolecules, electrical stimulation of damaged tissues,and particularly tissue engineering (bone, neural and vascular) and wound dressing. Our results haveproven supportive effects of nanocellulose/carbon nanotube composites on the adhesion and growthof human and porcine adipose tissue-derived stem cells, particularly under dynamic cultivation in apressure-generating lab-made bioreactor (see Appendix A). However, it should be pointed out that thebiomedical applications of nanocellulose/nanocarbon composites are associated with the risk of theirpotential cytotoxicity and immunogenicity, although this risk appears to be lower than for the singlecomponents of these materials.
Author Contributions: All authors have read and agree to the published version of the manuscript.Conceptualization, L.B., S.S. and A.S.; methodology, J.P., M.T., R.M., J.S., S.P., A.S., S.S.; software, R.M., J.S.,A.B.; validation, L.B., R.M.; formal analysis, L.B., A.B., A.S., S.S.; investigation, J.P., M.T., R.M., J.S., S.P., A.S.,S.S.; resources, L.B., P.K.; data curation, J.P., R.M., A.B., A.S.; writing—original draft preparation, L.B., J.P., R.M.;writing—review and editing, A.B., A.S., S.S., P.K.; visualization, J.P., M.T., R.M., A.B.; supervision, L.B., P.K.;project administration, L.B.; funding acquisition, L.B.
Funding: This research was funded by the Czech Science Foundation (grant no. 17-00885S). Another supportwas provided by the BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and CharlesUniversity project (CZ.1.05/1.1.00/02.0109), funded by the European Regional Development Fund.
Acknowledgments: Robin Healey (Czech Technical University, Prague) is gratefully acknowledged for hislanguage revision of the manuscript. Panu Lahtinen from VTT (Technical Research Center of Finland, Espoo,Finland) is acknowledged for providing nanocellulose for collaborative work between Tampere University ofTechnology and Institute of Physiology of the Czech Academy of Sciences.
Conflicts of Interest: The authors declare no conflict of interest. The funding sponsors had no role in the design ofthe study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decisionto publish the results.
Appendix A
In our own experiments, we have contributed to the knowledge on potential biomedicalapplications of nanocellulose/carbon nanotube composites, namely regarding their application in tissueengineering. In these experiments, a PurCotton® cellulose mesh (Winner Industrial Park, Shenzhen,China) was modified with an aqueous dispersion of positively-charged (i.e., cationic) wood-derivedCNFs, described in our earlier review article [2], and MWCNTs. Two types of composite sampleswere prepared, namely the samples with “thick” and “thin” coating of the fibers in the cellulosemesh. For thick coating, square-shaped samples of the cellulose mesh were fully immersed in anaqueous suspension of CNFs+MWCNTs, and during this immersion, the samples were homogeneouslyimpregnated with the nanoparticles. For thin coating, only one corner of the cellulose sampleswas submerged into the CNF+MWCNT suspension, which resulted in infiltration of the suspensionthroughout the cellulose mesh by capillary forces. Both types of samples were then dried for 5 h at 60◦C. Both types of samples displayed a grayish color, which was more intense in samples with a thickcoating. A pure CNF coating was prepared similarly to the thick coatings, and the meshes withoutcoating were used as a control.
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The samples were sterilized by UV light (20 min for each side), fixed into CellCrowns™ inserts(Scaffdex Ltd., Tampere, Finland), placed into 24-well cell culture plates (TPP, Trasadingen, Switzerland)and seeded with human adipose tissue-derived stem cells (hASC) or porcine adipose tissue-derivedstem cells (pASC). Human ASC were isolated from subcutaneous fat tissue, obtained by liposuctionfrom the abdominal region of healthy female donors after their informed consent, under ethical approvalissued by the Ethics Committee of Hospital “Na Bulovce” in Prague, and in compliance with the tenetsof the Declaration of Helsinki on experiments involving human tissues. The isolation was described inmore details in our earlier studies [173,174]. Porcine ASC were isolated by enzymatic disintegration ofsubcutaneous fat tissue samples obtained by excision from laboratory pigs in collaboration with theInstitute of Clinical and Experimental Medicine (IKEM) in Prague, Czech Republic. Characterizationof cells by flow cytometry revealed the positivity of cells for standard surface markers of ASCs, namelyCD105, CD90, CD73 and CD29 in hASC, and CD105, CD90, CD29 and CD44 in pASCs. The ASCs fromboth species were negative or almost negative for hematopoietic markers, such as CD34 and CD45,and for CD31, an endothelial marker [174,175].
The cells were seeded on the material samples at a density of 50,000 cells per well into 1.5 mL ofthe culture media. Human ASCs were cultivated in Dulbecco’s modified Eagle’s Medium (DMEM;Life Technologies, Gibco, Carlsbad, CA, USA) with 10% of fetal bovine serum (FBS; Life Technologies,Gibco), 40 µg/mL of gentamicin (LEK, Ljubljana, Slovenia) and 10 ng/mL of recombinant humanfibroblast growth factor basic (FGF2; GenScript, Piscataway, NJ, USA). Porcine ASCs were cultivatedin Dulbecco’s modified Eagle’s Medium (Low glucose, Sigma-Aldrich Co., St. Louis, MO, USA) andHam’s Nutrient Mixture F12 medium (DMEM/F 12, Sigma-Aldrich Co.) in a ratio of 1:1 with 10% offetal bovine serum (FBS; Life Technologies, Gibco), 1% Antibiotic Antimycotic solution (Sigma-AldrichCo.) and 10 ng/mL of recombinant human fibroblast growth factor basic (FGF2; GenScript).
After one or seven days of cultivation, the cells were fixed with 4% paraformaldehyde(Sigma-Aldrich Co.) for 20 min, and 0.1% Triton X-100 (Sigma-Aldrich Co.) diluted inphosphate-buffered saline (PBS) was applied for 20 min at room temperature in order to permeabilizethe cell membranes. Nonspecific binding sites for antibodies were then blocked by a solution of 1%bovine serum albumin and 0.1% Tween 20 in PBS (all Sigma-Aldrich Co.). Vinculin, a protein of focaladhesion plaques associated with integrin adhesion receptors, was visualized by treating the samplesfor 1 h at 37 ◦C with primary antibody against human vinculin (V9131, monoclonal mouse antibody,clone hVIN-1, Sigma-Aldrich Co.), diluted in the blocking solution (1% albumin and 0.1% Tween20 in PBS) in a ratio of 1:200. After washing with PBS, the samples were incubated for 1 h at roomtemperature in the dark with a secondary antibody, i.e., goat anti-mouse F(ab’)2 fragments of IgG (H +
L), conjugated with Alexa Fluor® 488 (A11017; Molecular Probes, Eugene, OR, USA; Thermo FisherScientific, Waltham, MA, USA), diluted in PBS to a ratio of 1:400. Finally, cytoskeletal F-actin filamentswere stained by phalloidin conjugated with tetramethylrhodamine isothiocynate (TRITC) fluorescentdye (Sigma-Aldrich Co.), diluted in PBS to a final concentration of 5 µg/mL, for 1 h at room temperaturein the dark. Microscopy images were acquired using spinning disk confocal system Dragonfly 503(Andor, Belfast, UK) with Zyla 4.2 PLUS sCMOS camera (Andor, Belfast, UK) mounted on microscopeLeica DMi8 (Leica Microsystems, Wetzlar, Germany) with objective HC PL APO 20x/0.75 IMM CORRCS2; Free Working Distance = 0.66 mm or HC PL APO 40x/1.10 W CORR CS2; Free Working Distance= 0.65 mm.
We found that the initial adhesion and subsequent growth of cells, evaluated by the cell numberand spreading on days 1 and 7, were similar on all coated samples. There was no apparent differencebetween samples coated with thick and thin layers of CNFs + MWCNTs and samples coated onlywith CNFs. However, all types of coatings markedly improved the adhesion and growth of cells incomparison with a pure uncoated cellulose mesh (Figure A1). In general, the cell growth was relativelyslow in all tested samples. On day 7, the cell number in all tested samples was only slightly higher thanon day 1. In addition, hASCs grew slightly better than pASCs, particularly on samples coated withCNFs+MWCNTs. Therefore, we decided to cultivate pASCs on the tested samples under dynamic
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conditions, which are known to improve the growth of cells by their mechanical stimulation, by abetter supply of nutrients and oxygen and by quicker waste removal.
Figure A1. Human adipose tissue-derived stem cells (hASC) and porcine adipose tissue-derived stemcells (pASC) on days 1 and 7 after seeding on a cellulose mesh with thick or thin CNF+MWCNT coating(column (a) and (b), respectively), with CNF coating (column (c)), and without any coating (column(d)). Cells were stained by immunofluorescence for vinculin (green), with TRITC-conjugated phalloidinfor F-actin (red) and with Hoechst #33258 for the nuclei (blue). Cellulose mesh had autofluorescencein the blue channel. Dragonfly 503 spinning disk confocal microscope with a Zyla 4.2 PLUS sCMOScamera, objective HC PL APO 20x/0.75 IMM CORR CS2. Scale bar: 50 µm.
The dynamic cultivation was held in a unique lab-made cultivation chamber (Figure A2). Thischamber allows for fixing a standard well plate with tested substrates and its hermetical sealing tomaintain the desired pressure. This chamber was connected to a custom pressure stimulator. Thisstimulator consists of a servo-controlled linear stage with piston pump and special controlling software.This software allows for setting stimulation parameters that include high/low pressure, motor speed,pulsatile frequency and the shape of the pressure wave.
Porcine ASCs were seeded on CellCrown-fixed substrates in 24-well plates at the same numberand in the same cultivation medium as mentioned above. Afterwards, the well plate for dynamiccultivation was fixed into the cultivation chamber, and this chamber was sealed and connected to thepressure stimulator. In the initial phase, the cells were left for 24 h without any pressure stimulationin order to allow their adhesion to the materials. The system was opened through a 220-nm filter toatmosphere forced with slow motion of pump piston to equilibrate CO2 level and pH of medium.After this resting phase, the pressure stimulation was set to 15.9/10.6 kPa (120/80 mmHg) high/lowpressure with frequency of 1 Hz (60 pulses per minute) with triangular pulse shape with 1:1 ratio. Thisdynamic cultivation lasted for 72 h (96 h of cultivation in total including the 24-h rest phase). Staticcontrol samples were cultivated for 96 h in a well plate with standard lid in the same CO2 incubator asthe dynamic samples.
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Figure A2. Lab-made dynamic cultivation system for pressure stimulation of cells on the tested materialsamples. The whole system in a cell incubator (a); detail of a cultivation chamber (b).
We found that dynamic cell cultivation markedly improved the adhesion and subsequent growthof pASCs. The cells were better spread and their number after three days of dynamic cultivation(day 4 after seeding) was markedly higher than in the corresponding samples incubated under staticconditions for four days (Figure A3), and also for seven days (Figure A1). The improvement incell colonization by dynamic cultivation was observed particularly on samples with thin CNF +
MWCNT coating. On both thick and thin CNF + MWCNT coatings, the cells under dynamic conditionswere distributed almost homogeneously, while on the pure CNF coating, the cells tended to formclusters. A similar picture was observed in our earlier study performed on human dermal fibroblastsin four-day-old static cultures on the same cationic CNFs, where the cells were less widespread anddistributed less homogeneously than on anionic CNFs [2]. Therefore, it can be concluded that theaddition of MWCNTs to cationic CNFs improved the colonization of the material with pASCs underdynamic cell culture conditions.
Figure A3. Porcine adipose tissue-derived stem cells (pASC) cultivated in a conventional static cellculture system for four days or in a pressure-generating dynamic cell culture system for three days(after one day of static cultivation). The cells were grown on a cellulose mesh with thick or thinCNF + MWCNT coating (column (a) and (b), respectively), with CNF coating (column (c)), andwithout any coating (column (d)). Cells were stained by immunofluorescence for vinculin (green), withTRITC-conjugated phalloidin for F-actin (red) and with DAPI for the nuclei (blue). Cellulose mesh hadautofluorescence in the blue channel. Dragonfly 503 spinning disk confocal microscope with a Zyla 4.2PLUS sCMOS camera, objective HC PL APO 20x/0.75 IMM CORR CS2. Scale bar: 50 µm.
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