Animal Biosafety Training: Core Concepts and Competencies ... · REGIONAL BIOCONTAINMENT LABORATORY...

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REGIONAL BIOCONTAINMENT LABORATORY

Animal Biosafety Training: Core Concepts and Competencies for Working with Animals in

ContainmentTimothy D. Mandrell, DVM, Dipl. ACLAM

Professor and ChairDepartment of Comparative Medicine

University of Tennessee Health Science Center

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Who needs training?

• Investigators• Research personnel• Animal technical staff• Animal care staff• Facility management

and operations• Facility support

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Required Training: All Personnel Who Work in Containment

• Animal Biosafety Levels• Animal Biosafety Risk Assessment and

Management• Animal Facility Design for Containment• Biocontainment Equipment• Biosecurity

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Required Training: All Personnel

• Concepts and Practices of Animal Biosafety: ABSL-2 and ABSL-3

• Incident Planning and Response• Institutional Responsibilities• Decontamination• Occupational Health• Select Agents and Toxins

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Required Training: Working with Animals in Containment

• Animal Husbandry• Animal Health• Biosecurity and cross contamination• Decontamination • Documents and recordkeeping

REGIONAL BIOCONTAINMENT LABORATORY

Required Training: Working with Animals in Containment

• Transport• Research procedures• Emergencies• Occupational health concerns

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Required Training: Working with Animals in Containment

• Basic and didactic training– AALAS Animal Biosafety Training

Program– CITI Biosafety and Biosecurity

Courses

• Facility Specific training– Emergency response– Decontamination– Agent specific– Animal specific

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The Challenge of Training Personnel on Animal Procedures

• Animal handling• Animal husbandry• Animal research procedures

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Program Responsibilities for Training

• Ensure training is effective

• Ensure that personnel who are trained understand and use that knowledge and skill properly

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Competency

Possession of required skill, knowledge, qualification or

capacity

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Competency Assessment or Evaluation

• Evaluates knowledge– Written Exam

• Demonstrate application of knowledge– Practical Exam – Direct observation for technical

skills and abilities*

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Animal Care and Use Training• Regulatory compliance• Species specific training• Animal technical

procedures– Handling and restraint– Anesthesia– Administration of test substances– Collection of biological samples

……….in containment.

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Progressive TrainingBasic procedures in learning friendly environment

– Animal lab– Bench top– Standard PPE

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Effective Animal Care Training

• Good learning environment

• Add additional degrees of complexity

• Simulate actual conditions

• Practice, practice, practice

• Trainer sign off

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Progressive Training

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Practice skills and increase complexity

• Benchtop with standard PPE

• Inside BSC with standard PPE

• Inside BSC with full PPE– Goggles/N95– PAPR

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Train and assess animal skills• Is the trainee comfortable handling animals?

• Does the trainee know how to perform procedures correctly?

• Does the trainee understand consequence of incorrect procedure?

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Training In Containment

• Initial training without live agent• Naïve animals• Progress to live agent and infected animals.• Buddy system and observation• Trainer signs off at each level

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Refresher Training

• Retraining• Reassessment

– Looking for deviation from SOP– Quality control– Documentation

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Never make assumptions

Technical competency with one procedure or species does not confer competency for another procedure or species.

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Animal Biosafety Training

• Progressive training• Practice makes perfect• Trainer sign off• Continuing assessment• Never Assume

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Thanks!

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