Adventures in HRP

Adventures in HRP

Ellyn DaughertySan Mateo Biotech Career Pathway www.SMBiotech.comAEEDaugher@aol.com www.BiotechEd.comEMC/Paradigm Publishing www.emcp.com/biotechVWR/Sargent-Welch www.sargentwelch.com

San Mateo Biotechnology Career Pathway www.SMBiotech.com

Giving motivated teenagers and adults the skills to pursue several career and academic options.

Partnerships with Industry and Academia

Biotechnology is an opportunity to teach science in a way that results in science literacy & research skills & career awareness for virtually every student.• Students learn the skills and gain the

confidence and self-directedness that are necessary for independent research(science fair projects) and higher-level science courses.

• Students see meaning and application in their math, biology and chemistry.

• Students learn the science and business of biotech so that they are better prepared to make decisions about their futures and society.

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• Text - Chapter 6 and 14• Lab Manual - Labs 6f-6h, 14a• Student CD Tutorials for basic skills• Course Planner/Instructor’s Guide

The HRP Activities are found in Chapter 6

Biotech 3 HRP labs:• Peroxidase Assay

• HRP extraction from HR Root

• HRP assay using PAGE

• HRP assay w/ TMB indicator

• HRP used in ELISAs

Hydrogen Peroxidase Activity, in Different Samples, Measured by Bubbling

Sample Amount of Bubbling (5-->0)

How Do You Make the Phosphate Buffer?

You need 100 mL of a 25 mM NaH2PO4 * H20, pH 7 buffer.You need 100 mL of a 25 mM NaH2PO4 * H20, pH 7 buffer.

0.345 g of NaH2PO4 * H20 is measured measured on a balance and put into an appropriate vessel. Then, solvent is added up to the 100 mL graduation.

0.345 g NaH2PO4 * H20

100 mL

add solvent (dH20) to 75 mLAdjust pH to 7 and add dH20 to 100 mL

conc. desired X volume desired X molecular weight = amount of solute in mol/L in L in g/mol in g

0.025 mol/L X 0.1 L X 138 g/mol = 0.345 g NaH2PO4 * H20

Convert 25 mM = 0.025 mM and convert 100 mL = 0.1 L

50 kD

35 kD

HRP is 43 kD

My Professional Goals

• Biotechnology programs in every high school, CTE center, and community college, tailored to the student population and industry and community needs

• Aligned and articulated programs in HS and CC so that teenagers and adults have the same platform from which to enter specialized programs

• To make it as easy as possible for educators to do the other 2

www.BiotechEd.com

www.BiotechEd.com

Get help starting or improving a biotech program !

Ellyn DaughertySM Biotech Career Pathway

www.SMBiotech.com

www.BiotechEd.com

www.emcp.com/biotech

www.sargentwelch.com/biotech

AEEDaugher@aol.com

JEFF GANDY 800.848.9033 jgandy@emcp.com

Holly Ahern512-789-3155holly_ahern @vwreducation.comSandy Duffey

for preview copy sduffey@emcp.com