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DOI:10.13350/j.cjpb.180314 ·!"
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】 16735234(2018)03027804
[犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔.2018Mar;13(3):278-281,286.]
犃狆狉犲犾犻犿犻狀犪狉狔狊狋狌犱狔狅犳狋犺犲犿犲犮犺犪狀犻狊犿犳狅狉狉犲犱狌犮犲犱犲犳犳犻犮犪犮狔狅犳犪狀犱狆狉狅狋犲犮狋犻狅狀犫狔犪犿犪犾犪狉犻犪狏犪犮犮犻狀犲
JIANGLinping1,GUANHongli
1,TENGPingying1,MOGang
1,LIDayu1,PENGXiaohong
1,2
(1.犇犲狆犪狉狋犿犲狀狋狅犳犘犪狉犪狊犻狋狅犾狅犵狔,犌狌犻犾犻狀犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋狔,犌狌犻犾犻狀,犌狌犪狀犵狓犻541004,犆犺犻狀犪;2.犇犲狆犪狉狋犿犲狀狋狅犳
犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔,犜犺犻狉犱犕犻犾犻狋犪狉狔犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋狔)
【犃犫狊狋狉犪犮狋】 犗犫犼犲犮狋犻狏犲狊 Toinvestigatethemechanismforreducedefficacyofandprotectionbyamalariavaccine.
犕犲狋犺狅犱狊 C57BL/6orBALB/cmicewereintravenouslyinoculated3timeswith10kor50kWT犘犾犪狊犿狅犱犻狌犿狔狅犲犾犻犻spo
rozoites(spz)at4weekintervals.Threehoursafterinjection,themiceweretreatedwith0.8mgofchloroquine(CQ)
for10consecutivedays.Micewerechallengedwith10kWTspzthatwereinjectedintravenously30,90,or180dafter
immunization.Thephenotypeofhepaticlymphocyteswasdeterminedwithflowcytometry. 犚犲狊狌犾狋狊 BothC57BL/6and
BALB/cmicehad100% protection30dafterimmunization.However,10% ofC57BL/6mice(1/10)haddetectable
bloodstageparasitemia90dafterimmunization,and60%ofC57BL/6mice(6/10)hadparasitemia180dafterimmuniza
tion.AlloftheBALB/cmicehad100%protection180dafterimmunization.Phenotypicanalysisoflymphocytesindica
tedthatPD1expressiononCD8+TcellsincreasedsignificantlyinC57BL/6mice90dafterimmunizationbutnotin
BALB/cmice.Moreover,thelevelofPD1expressioninmice180dafterimmunizationwassignificantlyhigherthanthat
inmice90dafterimmunization(狋=6.175,犘<0.01). 犆狅狀犮犾狌狊犻狅狀 Thereducedefficacyofandprotectionbymalaria
vaccinewascorrelatedwithahighlevelofPD1expressiononCD8+Tcells.
【犓犲狔狑狅狉犱狊】 犘犾犪狊犿狅犱犻狌犿狔狅犲犾犻犻;vaccine;CD8+Tcells;PD1
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)
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犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔 2018ñ3ò '13ó'3h
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犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔 2018ñ3ò '13ó'3h
March2018,Vol.13,No.3
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