Active Transport. Cellular energy is used to to transport substances across the membrane against a...

Active Transport

Active Transport

Cellular energy is used to to transport substances across the membrane against a concentration gradient

Energy is derived from splitting ATP

Pumps

A transporter protein that uses energy from splitting ATP to change shape and carry a substance across a cellular membrane against its concentration gradient

Substances Transported

Na+

K+

H+

Ca++

I-

Cl-

Sodium-Potassium Pump

Expels Na+ from the cell, brings K+ into the cell

Acts as an enzyme to split ATP

Used to maintain a low [Na+] concentration in the cytosol by pumping Na+ into the extracellular fluid

Continuous Operation

Na+ and K+ slowly leak back across the membrane down their gradients, so the pumps must continuously move these ions back across the membrane to maintain the concentration gradient.

Concentration Gradient

The differing concentrations are crucial for osmotic balance of the 2 fluids and also for the ability of some cells to generate electrical signals

Operation of the Sodium Potassium Pump

1. Three Na+ in the cytosol bind to the pump protein2. Na+ binding triggers the splitting of ATP into ADP plus a phosphate group which attaches to the pump protein. This causes a change in the shape of the pump protein3. Three Na+ are expelled into the extracellular fluid

Operation of the Sodium Potassium Pump

4. The changed shape allows 2 K+ in the extracellular fluid to bind to the protein5. The binding of the K+ causes the phosphate group to be released which causes the pump protein to return to its original shape6. As the pump protein returns to its original shape, it releases the 2 K+ into the cytosol.

The pump protein is then ready to bind Na+ and the cycle can repeat

Malfunctions of Transporters

Drugs – some turn off ATP production which would stop active transport of substances throughout the body

Cystic Fibrosis – a defective gene causes an abnormal Cl- transporter. There is a failure to secrete adequate amounts of Cl- resulting in extremely thick mucus secretions that result in obstruction and infection.

Transport in Vesicles

Vesicle – a small sac formed by the budding off from an existing membrane

Movement of vesicles requires energy supplied by ATP.

ENDOCYTOSIS

Materials move into a cell is a vesicle formed from the plasma membrane

Phagocytosis

Bulk Phase Endocytosis

Phagocytosis

Large solid particles (like whole cells) are taken in by the cell.

The particle binds to the plasma membrane receptor, the cell extends projections called pseudopods that surround the particle, then the membranes fuse to create a vesicle in the cytoplasm.

Any undigested material remain indefinitely in a vesicle called a residual body

Phagocytosis occurs only in special cells called phagocytes

They are specialized cells used to engulf and destroy bacterial and other foreign substances

• These cells include certain white blood cells and macrophages.

• Phagocytosis is one of the body’s defense mechanisms against disease

Bulk Phase Endocytosis

Cells take up vesicles containing tiny droplets of extracellular fluid and any solutes dissolved in that fluid

The vesicle detaches from the membrane and enters the cytosol

The vesicle fuses with a lysosome where enzymes digest the solutes into smaller molecules

Exocytosis

Results in secretion – materials exiting the cell

Secretory cells give off digestive enzymes, hormones, mucus or other secretions

Nerve cells give off neurotransmitters

Secretory Vesicle

Vesicle containing substance to be secreted forms in the cytosol, fuses with the plasma membrane then releases the contents into the extracellular fluid

CHECKPOINT

What is the key difference between active and passive transport?Briefly compare/contrast facilitated diffusion and active transport through a membrane pumpDescribe the process of phagocytosis.Name one way phagocytosis helps to maintain homeostasis.