Active thermodynamics of cell membranes.indico.ictp.it/event/a05198/session/8/contribution/6/... ·...

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Active thermodynamics of Active thermodynamics of cell membranescell membranes

NirNir GovGovDepartment of Chemical PhysicsDepartment of Chemical Physics

Workshop on Driven States in SoftWorkshop on Driven States in Softand Biological Matterand Biological Matter

ICTP, April 18ICTP, April 18--28, 200628, 2006

PlanPlan

Introduction: Introduction: MicrovilliMicrovilliMicrovilliMicrovilli height distributionheight distributionMicrovilliMicrovilli organization and morphologyorganization and morphologyConclusions and future directions: Conclusions and future directions: NeuronsNeurons

Introduction: Introduction: MicrovilliMicrovilli

J. J. GorelikGorelik et. al.et. al. ; PNAS ; PNAS 100100, 5819, 5819––5822 (2003)5822 (2003)

S. S. MajstoravichMajstoravich et. al.et. al. ;;BLOOD (2004) BLOOD (2004) 104104, 1396., 1396.

J. J. GorelikGorelik et. al.et. al. ;;Mol. Cell. Endo. Mol. Cell. Endo. 217217 (2004) 101(2004) 101

ActinActin bundles:bundles:

Dynamic:Dynamic:

MicrovilliMicrovilliSchematic picture:Schematic picture:

ActinActin polymerization driven motionpolymerization driven motion

ActinActin treadmillingtreadmilling

InstabilityInstability→→ FilopodiaFilopodia

Our model: link Our model: link actinactin polymerization polymerization with membrane curvaturewith membrane curvature

FilopodiaFilopodia:: Membrane proteins at the tipMembrane proteins at the tip

PhosphoPhospho--tyrosinetyrosine(PY), Cdc42, (PY), Cdc42, SrcSrcetc. etc.

Do these active Do these active proteins have a proteins have a positive positive spontaneous spontaneous curvature ?curvature ?

FilopodialFilopodial tip complextip complex

••Also important Also important are bundling are bundling proteins such as proteins such as FascinFascin••Tip contains Tip contains forminsformins, , protecting protecting actinactinfrom cappingfrom capping

Restoring force: saturationRestoring force: saturationLhc ∝

RF /0 κ≈

“Long cylinder” limit

Equation of motionEquation of motion

+−=∂∂ ω

Where: Where: chF /0∝ω

nAv =0

And a noise term: And a noise term:

( ) 02 ≠− nn

Restoring force Restoring force

Growth velocity Growth velocity

MicrovilliMicrovilli: Height distribution: Height distribution

ObservationsObservations::

MicrovilliMicrovilli: Height probability distribution: Height probability distribution

( ) nAhPnA

∂∂

−∂∂

+∂∂

=∂∂ 2

From the equation of motionFrom the equation of motion→→ FokkerFokker--Planck Planck equequ.:.:

hh DhDhvn eeRhP //2 2

0)( ω−=⇒At steadyAt steady--state westate weget:get:

where:where: ( ) ωω /// 20

22 vnnnADh ∆==

Describes the distribution of tip activityDescribes the distribution of tip activity

0/ =∂∂ tP

Fluctuations in the size of the MV tipFluctuations in the size of the MV tip

Aggregation driven by spontaneous curvature:Aggregation driven by spontaneous curvature:

κκTk

nETkn BB

B ≈⎟⎟⎠

⎞⎜⎜⎝

⎛∆⇒≈

∂∂=

Typically Typically κκ~5~5--20 20 kkBBTT

2Rn∝

Thermal Thermal noise !noise !

MicrovilliMicrovilli: Height probability distribution: Height probability distribution

MV height (nm)MV height (nm)

Why narrow distributionin ridges ?

MicrovilliMicrovilli: Spatial distribution/patterns: Spatial distribution/patterns

A. Sharma A. Sharma et. al.et. al. ;;FEBS Letters FEBS Letters 579579 (2005) 2001(2005) 2001

RhEcurvκ

≈Reduced membrane curvature Reduced membrane curvature energy between the MV:energy between the MV:

≈bendEIncreased curvature energy of Increased curvature energy of flattened tip:flattened tip:

Min. height for ridge:Min. height for ridge: RhEE ridgebendcurv 8π

≈⇒≥

MVMV--MV MV attractive attractive interactions:interactions:

MicrovilliMicrovilli: ridges & height distribution: ridges & height distribution

MV of unequal heights attract each other MV of unequal heights attract each other less then MV of equal heightsless then MV of equal heights

( )nnhh −−ωAdditional restoring force:Additional restoring force:

MV in ridges: Height prob. distributionMV in ridges: Height prob. distribution

Ridge height equationRidge height equationof motion:of motion:

( )nnhhAnhth

−−+−=∂∂ ωω 0

MeanMean--field:field:hhnn ≈

S. S. MajstoravichMajstoravich et. al.et. al. ; BLOOD (2004) ; BLOOD (2004) 104104, 1396., 1396.

Exponential tails: due to force saturationExponential tails: due to force saturation

Long Long MicrovilliMicrovilli: Force saturation: Force saturation

Gaussian Gaussian replaced by replaced by long long exponential tailexponential tail

chnAh <≈ ω/

Long MV: Height probability distributionLong MV: Height probability distribution

A. Sharma A. Sharma et. al.et. al. ;;FEBS Letters FEBS Letters 579579 (2005) 2001(2005) 2001

•• Dynamic Dynamic →→ TTeffeff•• ““ThermodynamicThermodynamic

”” phase diagramphase diagram

MicrovilliMicrovilli: Spatial distribution/patterns: Spatial distribution/patterns•• Linear aggregates due to Linear aggregates due to positive spontaneous curvature positive spontaneous curvature of tip complexof tip complex

T. T. TlustyTlusty & S. & S. SafranSafran; Science ; Science 290290 (2000) 1328(2000) 1328

2D dipolar fluid, 2D dipolar fluid, network of network of wormworm--like like

micelles etc.micelles etc.

•• Assume single height of MV: <h>Assume single height of MV: <h>•• Excluded volume interactionsExcluded volume interactions•• Defects: free ends and 3Defects: free ends and 3--fold junctionsfold junctions

Based on:Based on:ZilmanZilman, A; , A; SafranSafran, S, S..AA..; ; SottmannSottmann, T, T..; ; StreyStrey, R, R..; ; LangmuirLangmuir,, 2020 21992199 ((20042004).).

MicrovilliMicrovilli: Energy of defects: Energy of defects

82 πκπκ

−≈RhEend

6πκπκ

+≈RhEjunct

Increases Increases with with hh

Increases Increases more slowly more slowly with with hh

Formation of networksFormation of networksIf the MV height increases, junctions multiply If the MV height increases, junctions multiply over ends: over ends:

Phase Phase transition to transition to a a connected connected network: network:

SpinodalSpinodal

Minimize with respect to independent Minimize with respect to independent defectsdefects’’ concentrations:concentrations:

Free energy of gas of defectsFree energy of gas of defects

ФФ is the area fraction of the MVis the area fraction of the MVФФee & & ФФjj is the area fraction of the ends is the area fraction of the ends and 3and 3--fold junctions respectivelyfold junctions respectively

Using: Using: κκ=10 =10 kkBBTT, , hh=400nm=400nmPercolation line

“Gas”

Coexistence

Network “liquid”

Note: Large Note: Large ““effectiveeffective”” temperaturetemperature

Using: Using: κκ=10 =10 kkBBTT, , ΦΦ=0.05=0.05

Rh ridge 8π

Percolation line

heightheight--density phase diagramdensity phase diagram

Using a fixed Using a fixed TTeffeff

Does it really behave as Does it really behave as ““activeactive””--thermodynamics ?thermodynamics ?

Predictions of Predictions of thermodynamic theory:thermodynamic theory:

2/32/ : where,~)( /

ll lelPφφ

(a)(a)

(b)(b)

J. J. GorelikGorelik et. al.et. al.

•• Phase Phase separation ?separation ?•• What What changes changes between cells between cells ??•• Equilibrium Equilibrium between between systems in systems in contact ?contact ?

ConclusionsConclusions

Spontaneous curvature of membrane Spontaneous curvature of membrane proteinsproteins that that activate activate actinactinpolymerizationpolymerization drives drives MicrovilliMicrovillidynamics dynamics andand morphologymorphology..Coupling of Coupling of activeactive and and thermalthermalfluctuationsfluctuations on different length and on different length and time scales: time scales: ““ActiveActive--ThermodynamicsThermodynamics””

AcknowledgementsAcknowledgementsTheoryTheoryAjay Ajay GopinathanGopinathan

(UCSB) (UCSB) Sam Sam SafranSafran (WIS)(WIS)Jacques Prost (Curie)Jacques Prost (Curie)

FundingFundingBSF, EUBSF, EU--NoENoE, ISF, ISF

ExperimentExperimentErich Erich SackmannSackmann (TUM)(TUM)Benny Geiger (WIS)Benny Geiger (WIS)RonenRonen AlonAlon (WIS)(WIS)

MultiMulti--scale Modelingscale Modeling

Thermal & actin-driven aggregation → Tip formation

~100nm

Thermal & actin-driven aggregation of MV → Network formation

Cell-wide morphology and shape

~10µm

We need to characterize the We need to characterize the physical parameters of the physical parameters of the protein aggregates at the protein aggregates at the membrane:membrane:

What is the spontaneous What is the spontaneous curvature ?curvature ?

Experimental challenge:Experimental challenge:

Active thermodynamics of cell membranes.indico.ictp.it/event/a05198/session/8/contribution/6/... ·...

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