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22/10/2008
Met52Bio-insecticide
Metarhizium anisopliae Strain F52
Thierry Pradier
Novozymes Biologicals
NOVOZYMES PRESENTATION22/10/20082
Overview
Brief M. anisopliae Cycle
Product Description
Efficacy and Related Data
Major targets
Secondary targets
Summary
NOVOZYMES PRESENTATION22/10/20083
Simplified Life Cycle of Metarhizium anisopliae
Saprophyte in Soil
Infection throughthe Cuticle
Mortality and Sporulation
Horizontal Transmission
NOVOZYMES PRESENTATION22/10/20084
Product Description
2 formulations
Granular Formulation on rice for soil applications
EC Formulation for spray
NOVOZYMES PRESENTATION22/10/20085
Metarhizium has demonstrated some control on:• Black Vine Weevil• Blacklegged Ticks• Greenhouse Whitefly• Green Peach Aphid• Two Spotted Spider Mite• Western Flower Thrip• Root Maggots (cabbage, sugarbeet)• Cotton Aphid• Japanese Beetle
Met52tm Bioinsecticide : Spectrum of activity
NOVOZYMES PRESENTATION22/10/20086
1st Major Targets
Denny Bruck, USDA-ARS
Denny Bruck, USDA-ARS Kirby Stafford, CT Ag. Expt. Station
Kirby Staffor, CT Ag. Expt. Station
Black Vine Weevil Ticks
NOVOZYMES PRESENTATION22/10/20087
Worldwide Distribution of Black Vine Weevil
150+ Host Plant Species
NOVOZYMES PRESENTATION22/10/20088
Competitive Efficacy with Chemical Standards(Black Vine Weevil)
Control Met52 G1g/L
Met52 G2g/L
Met52 G5g/L
Bifenthrine40 oz/100gal
Bendiocarb4 oz/
100 gal
Imidacloprid32 g/
100gal
0
5
10
15
20
Liv
e L
arv
ae /
Po
t (3
wk P
ost
-Trt
)
Dan Gilrein, 1999, Cornell Cooperative Extension
NOVOZYMES PRESENTATION22/10/20089
Persistent Control of Black Vine Weevil in Potting Media
5
5.5
6
6.5
7
7.5
0 50 100 150 200 250 300 350Days Post Fungus Application
Log 1
0C
FU/g
of P
ottin
g M
edia
Bruck (2005) Biological Control 32:155-163
97.5% Infection ofLast Instar BVW
NOVOZYMES PRESENTATION22/10/200810
Temperature Optima of M. anisopliae F52
Moorhouse et al. (1994) Ann Appl. Biol. 124:185-193
0
5
10
15
20
25
30
35
10° C(50° F)
15° C(59° F)
20° C(68° F)
25° C(77° F)
LT50
(Day
s) B
VW L
arva
e
NOVOZYMES PRESENTATION22/10/200811
Fungicide Compatibility of M. anisopliae F52
0
10
20
30
40
50
60
70
80
90
100
Met
52
Ben
omyl
+Met
52
Bup
irim
ate+
Met
52
Car
bend
azim
+Met
52
Chl
orot
halo
nil+
Met
52
Etrid
iazo
le+M
et52
Fena
rimol
+Met
52
Fura
laxy
l+M
et52
Ipro
dion
e+M
et52
Prop
amoc
arb+
Met
52
Pyra
zaph
os+M
et52
Tolc
lofo
s-m
ethy
l+M
et52
Zine
b+M
et52
Moorhouse et al. (1992) Biocontrol Sci. Technol. 2:49-58
% C
ontr
ol o
f Bla
ck V
ine
Wee
vil
NOVOZYMES PRESENTATION22/10/200812
1st Major Targets
Denny Bruck, USDA-ARS
Denny Bruck, USDA-ARS Kirby Stafford, CT Ag. Expt. Station
Kirby Staffor, CT Ag. Expt. Station
Black Vine Weevil Ticks
NOVOZYMES PRESENTATION22/10/200813
Worldwide Distribution of Lyme Disease
NOVOZYMES PRESENTATION22/10/200814
Integration of M. anisopliae into Tick IPM
Stafford (2004) Tick Control Handbook. CT. Ag. Expt. Station Publication
NOVOZYMES PRESENTATION22/10/200815
Control of Black Legged Ticks with Tick-EX EC New Jersey Field Trial
Days Post Treatment
Ave
rage
Tic
ks p
er D
rag
0
10
20
30
40
50
60
70
80
0 7 14 21 28 35 42 49 56 63 70 77 84
Control-NymphTick EX EC-NymphControl-LarvaeTick EX EC-Larvae
Single Application at 1X
NOVOZYMES PRESENTATION22/10/200816
M. anisopliae F52 Persistence in Tick Environments New Jersey Field Trial
0.00E+00
5.00E+04
1.00E+05
1.50E+05
2.00E+05
2.50E+05
3.00E+05
3.50E+05
Jun 5 Jun 12 Jun 19 Jun 26 Jul 3 Jul 10
Date
CFU
's/g
of le
af li
tter
Treated
Control
NOVOZYMES PRESENTATION22/10/200817
Greenhouse and Field Trials
Whiteflies
Thrips:Wireworms
Grubs:Root Maggots:
Leaf-Feeding Beetles
Cockroaches
Mites
Weevils
Borers
Ticks
Nursery and OrnamentalsLawn and Landscape PCO
ForestrySustainableAgricultureApiculture
Flies
NOVOZYMES PRESENTATION22/10/200818
Greenhouse and Field TrialsNursery and Ornamentals• Black vine weevil, Japanese Beetle, Oriental Beetle• Whiteflies, thrips, mites, aphids• Leaf-feeding beetles• Scale, mealybugs
Lawn and Landscape•Ticks•Turf Grubs• Crane flies• Chinch bugs• Billbugs
PCO• Cockroaches• Ants
Forestry• Poplar plantations• Tick control• Asian longhorn beetle
SustainableAgriculture• Vegetables• Berries• Tree Fruits/Nuts• Root crops• Organic
Apiculture• Small hive beetle• Varroa mite
• 90 Field Trials• 40 Insect Targets
NOVOZYMES PRESENTATION22/10/200819
Whitefly Control with Met52 EC
0
20
40
60
80
100
120
140
160
180
0 14 28
Days
Tota
l Whi
tefli
es p
er L
eaf
ControlMet52 LowMet52 MidMet52 High
NOVOZYMES PRESENTATION22/10/200820
Two-Spotted Spider Mite Control with Met52 EC
0
2
4
6
8
10
12
14
0 14 28Days
Mite
s pe
r Lea
f
ControlM et52 LowM et52 High
NOVOZYMES PRESENTATION22/10/200821
Thrip Control and Onion Yield from Met52 EC
0
10
20
30
40
50
60
70
80
Thrips/plant Foliar damage % larger onions (#) % larger onions(wt.)
Met52 ECControl
NOVOZYMES PRESENTATION22/10/200822
Potential for Thrip Pupae Control in Soil
Ma=Met52Imi=ImidaclopridFip=FipronilFC= Full ConcentrationSLD=Sub-lethal DosePM= PremixedDR=Drench Ansari et al (2007) Biological Control. 40: 293-297
NOVOZYMES PRESENTATION22/10/200823
Mole Crickets – Laboratory Bioassays
0102030405060708090
100
0 2 4 6 8
Days
% M
orta
lity
4x10E5 spores/mL 4x10E6 spores/mL4x10E7 spores/mL Untreated Control
NOVOZYMES PRESENTATION22/10/200824
Western Corn Rootworm
0102030405060708090
100
Met52ECat 2x104
Met52ECat 2x105
Met52ECat 2x106
Met52G at3x106
Met52G at3x107
Control
Spores per Gram of Soil
% M
orta
lity
NOVOZYMES PRESENTATION22/10/200825
Summary
Granular and EC formulations available
Proven efficacy by independent researchers
Residual soil activity (one year in potting media)
Expanding labels for granular and EC to include food use and wider pest spectrum
Registered in USA and Holland
EU registration process currently going-on (on active ingredient forth list): decision for Annex 1 inclusion end 2008 – early 2009
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