1. Bacillus cereus - Rutgers Universitymicrolab/applied micro/schedulelinks/applied...1. Bacillus...

EVALUATION OF IMMUNOLOGICAL DETECTION METHODS

1. Bacillus cereus toxin detectiona. In-class methodb. “Oxoid”

http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=TD0950&c=UK&lang=EN

c. “Tecra”http://www.tecra.net/products/bacillus_diarrhoeal_enterotoxin(see PDF on SAKAI for info re above kits)2. Escherichia coli 0157 detection

a. Reveal®b. GeneQuence E. coli®

3. Salmonella detection (Neogen)Reveal®

Evaluate these methods for the detection of three different organisms. For each method answer the following questions: (This may be done in table format or another way that works for you.)

•What is the “target” molecule in each test? (i.e. What is being detected?)•What is immobilized (stationary) in each of these methods?•What is the purpose of immobilization?•What role(s) do antibodies play in each test?•What is the means of visualization in each test? (i.e. What do we see?) NOTE: I think this will be speculative for GeneQuence E. coli®as I can’t find the information on the Neogen web site – maybe you can do better!•Why didn’t I include the Salmonella GeneQuence® kit on the list above?

Negative Elisa Test

(96 well)

An Example of The working principle behind Immunodetection Assays