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Bihar Medical Services & Infrastructure Corporation l'imitc+l
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Hospital Road, Shastri Nagar, Patna 800023'
Phone/Fax z +91612 2283287,+ 91612 2283288
Public otice Inviti aims/Obiections rdin Procurement of
COVID Test Kit for ltull tomated DNA/RN Bx n Mach
Roche" M A Pure- BMSIC Patna
Proprietarv Basis.
BMSICL Patna is going to procure covlD Test Kit for Fully Automated DNA/RNA Extraction
Machine (Make- RochelModel- MagNA Pure-96) on Proprietary basis' The proposal subrnitted by
M/s Roche Diagnostics India Pvt. Ltd. and its Propriety Article Certificate have been attached &
uploaded on website of BMSICL.
.l-lre above docurnents are being uploaded for open information to sLrbmit Clairns/Objectiotts, if any
fi"om a.y Manufacturer/Distributoi regarding pioprietary nature_ and pricing of the above mentiottcd
kits/con"sumables within 7 days front the date of publication o1'this notice'
The comments should be sent to BMSICL Patna on or before l5'0'/'2020 up to 3'00 PM tliroLrgh
emaif to md:b-msigl:bih@nic'in or gmproc'bmsicl@Smail'com or
bmsicl.equipmen@gmail.com, failing wlricli it will be presumed that no one is having arry
Clairns/Otiection to-oTfer regarding this and the case will be decided on merits'
Ad't.t\6-1'*'GM (Procurement)
BMSICL. Patna
Page 1 of 1
MagNA Pure 96 System
Start here. Go anywhere.
The MagNA Pure 96 System is a high-throughput instrument for
automated nucleic acid purification. Typical run duration is less than
one hour for 96 standard volume samples. Experience high throughput
automation combined with high performance while increasing your
laboratory productivity to meet your changing needs.
• Isolate DNA, RNA, and viral nucleic acids from a wide range of starting materials.
• Simplify your workflow with pre-programmed protocols tailored to different sample
requirements.
• Utilize proven magnetic glass particle technologies.
• Rely on eluates suitable for a broad range of genomic applications.
• Magnify your lab’s efficiency with seamless data management.
• Expand capacity for diverse day to day throughput needs.
Start here.
Go anywhere
with confidence.
“The MagNA Pure 96 helps us to maximize staff productivity, decrease
our turnaround times, and accommodate fluctuations in testing demands
at the peak of respiratory virus season without making significant
changes to staffing and workflows. Overall, we are very happy with
the MagNA Pure 96 system and with our Roche partnership.”
MagNA Pure 96 Reagent Kits Prefilled, barcoded, and ready-to use
MagNA Pure 96 Kits are prefilled and ready-to-use reagent trays that can be easily loaded
into racks, ensuring user ease and safety. These kits, as well as the consumables, are barcoded
and read by the MagNA Pure onboard scanner for inventory check, loading errors, and
process documentation.
Three kits that cover a large range of starting materials and targets
Reagents Target
Starting Material
Wh
ole
blo
od
Pla
sma
Cell
Cu
lture
Seru
m
Bo
dy
fluid
s
Fresh
fro
zen
tis
sue
FFP
E t
issu
e
Sw
ab
Sto
ol
Sp
utu
m
MagNA Pure 96 DNA and Viral Nucleic Acid
Small Volume Kit
Genomic DNA ● ● ● ●
Bacterial DNA ● ● ● ● ● ● ● ●
Viral DNA/RNA ● ● ● ● ● ● ● ●
MagNA Pure 96 DNA and Viral Nucleic Acid
Large Volume Kit
Genomic DNA ● ● ●
Bacterial DNA ● ● ● ● ● ● ● ●
Viral DNA/RNA ● ● ● ● ● ● ● ●
Cell free NA ●
MagNA Pure 96 Cellular RNA Large Volume Kit* Total RNA ● ● ● ●
MagNA Pure 96 Software Intuitive user interface
The intuitive graphical user interface provides all instructions to
operate the system, such as sample loading to run start. The software
detects loading errors and provides information to the user for the
appropriate corrective action.
* For life science research only. Not for use in diagnostic procedures.
C. Renee Webb, MT(ASCP)
Manager, Molecular Laboratories
Texas Children’s Hospital
Anatomy of a MagNA Pure 96 System Effortless purification, confidence from automation
Proven performance
� Over 20 years of successful magnetic bead
technology
� High quality extract with reproducible and
consistent yields
� GMP-manufactured and IVD registered
� Dedicated, experienced support and
service teams
Simplified Workflow
� Ready-to-use prefilled reagents
� Minimal consumables
� On-board barcode scanning for inventory
� Streamlined kit offering that purify a variety
of nucleic acids
� Intuitive graphical interface
Contamination Prevention
� UV lamp
� Drop catcher
� CO-RE Tip technology with stable lock-and-key fit
which eliminates the risk of cross-contamination
Accelerated Results
� 96 samples in less than one hour
� Parallel handling of all samples with 2 robotic arms
� Set-up in less than 5 minutes
Data Management
� Software with audit trail and user management
� LIMS host connectivity
Optimized Productivity
� Broad range of supported samples
� Pre-programmed protocols, including “Pathogen
Universal” that extracts DNA and RNA from 10
common samples
� Eluate used for a variety of downstream applications
Minimized Handling Error
� Fully automated workflow
� Error detection with corrective action guidance
� Deck and volume surveillance
� Barcoding of kits and consumables
Flexibility for optimized productivityEfficient Purification
Experiment details
Kit: MagNA Pure 96 DNA and Viral NA SV Kit
Protocol: SV Blood protocol
Sample: Whole blood with various white blood
cell counts ( 3700, 5800, 10600 K/µL)
DNA was measured with the UV-spectrophotometer. The alignment of the duplicate curves for
the varying cell count indicates reproducibility and scalability of the MagNA Pure 96 Instrument.
Flexibility along with convenience
A variety of targets from a wide range of
sample types can be extracted efficiently on
the MagNA Pure 96 System using one protocol,
one kit, and within the same run as indicated
by the crossing points detected on the Roche
LightCycler® 480 Real-Time PCR Instrument.
Experiment details
Kit: MagNA Pure 96 DNA and Viral NA LV Kit
Pretreatment: As described in Instructions for Use
Protocol: Pathogen Universal 1000
Sample: EDTA plasma, throat wash, swab, cerebrospinal fluid, bronchoalveolar lavage
Pathogen Spike-in: Hepatitis A virus (RNA virus), Parvo B19 virus (DNA virus), Staphylococcus aureus and
Enterococcus faecalis (gram positive bacteria) , β-globin (control)
40.00
35.00
30.00
25.00
20.00
15.00
10.00
5.00
0.00Plasma Throat Swab CSF BAL
Cro
ssin
g P
oin
t
Hep A Parvo B19 S. aureus E. faecalis βGlobin
“My lab techs have been so happy with the transition to the MagNA
Pure 96. Setup takes less than 5 minutes and is very easy. We have
moved almost all of our extractions to this instrument, and – across
our sample types – it gives us highly consistent eluates.”
2.0
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0220 230 240 250 260 270 280 290 300 310 320
Ab
so
rba
nc
e (
AU
)
Wavelength (nm)
10600 K/µl 5800 K/µl 3700 K/µl
β-globin
Increase efficiency in pathogen detection
The Roche workflow makes pathogen detection reliable and consistent. The high throughput
MagNA Pure 96 System dramatically speeds up extraction by allowing different sample types
to be supported by the same kit and protocols which enables faster downstream analysis.
Increase efficiency even further with multiplex qPCR and assay panels offered by TIB MolBiol
which can be run the LightCycler® 480 instrument.
Experiment details
Kit: MagNA Pure 96 DNA and Viral NA SV Kit
Pretreatment: As described in IFU
Protocol: Pathogen Universal
Sample: Serum (Parasite panel), Stool (Gastro panel), and Swab (Influenza panel)
Parasite Panel Channel Cp Std Hits
E. Histrolytica 440-488 29.6 0.1 2/2
Giardia 465-510 30.0 0.0 2/2
Dientamoeba 533-580 29.7 0.1 2/2
Cryptosporidium 533-610 29.6 0.1 2/2
Blastocystis 533-640 30.1 0.1 2/2
Gastro Panel Channel Cp Std Hits
Norovirus GG2 465-510 35.1 0.2 2/2
Rotavirus A 533-580 33.0 0.0 2/2
Adenovirus V 533-610 29.2 0.0 2/2
Flu Panel Channel Cp Std Hits
Influenza A 465-510 33.6 0.2 2/2
Influenza B 533-580 33.7 0.5 2/2
Safedin Sajo Beqaj, Ph.D., HCLD, CC (ABB)
Scientific Director
Pathology, Inc.
310.540
280.540
250.540
230.540
190.540
160.540
130.540
100.54
70.540
40.540
10.5405 10 15 20 25 30 35 40 45
Cycles
Flu
ore
sc
en
ce
(4
40
-48
8)
E. Histolytica
Amplification Curves
5 10 15 20 25 30 35 40 455 10 15 20 25 30 35 40 45
54.890
49.890
44.890
39.890
34.890
29.890
24.890
19.890
14.890
9.890
4.890
-0.1105 10 15 20 25 30 35 40 45
Cycles
Flu
ore
sc
en
ce
(4
95
-51
0)
Giardia
Amplification Curves
84.465
76.465
68.465
60.465
52.465
44.465
36.465
28.465
20.465
12.465
4.4655 10 15 20 25 30 35 40 45
Cycles
Flu
ore
sc
en
ce
(5
33
-58
0)
Dientamoeba
Amplification Curves
84.465
76.465
68.465
60.465
52.465
44.465
36.465
28.465
20.465
12.465
4.4655 10 15 20 25 30 35 40 45
Cycles
Flu
ore
sc
en
ce
(5
33
-58
0)
Dientamoeba
Amplification Curves
Dientamoeba62.552
56.552
50.552
44.552
38.552
32.552
26.552
20.552
14.552
8.552
2.5525 10 15 20 25 30 35 40 45
Cycles
Flu
ore
sc
en
ce
(5
33
-61
0)
Amplification Curves
Cryptosporidium60.314
54.314
48.314
42.314
36.314
30.314
24.314
18.314
12.314
6.314
0.3145 10 15 20 25 30 35 40 45
Cycles
Flu
ore
sc
en
ce
(5
33
-64
0)
Amplification Curves
Blastocystis
34.438
31.438
28.438
25.438
22.438
19.438
16.438
13.438
10.438
7.438
4.438
1.438
Cycles
Flu
ore
sc
en
ce
(4
65
-51
0)
5 10 15 20 25 30 35 40 45
GG2
Amplification Curves
13.130
12.130
11.130
10.130
9.130
8.130
7.130
6.130
5.130
4.130
3.130
2.130
1.130
0.130
Cycles
Flu
ore
sc
en
ce
(5
33
-58
0)
5 10 15 20 25 30 35 40 45
RotaA
Amplification Curves
16.944
15.944
13.944
12.944
10.944
9.944
7.944
6.944
4.944
3.944
1.944
0.444
Cycles
Flu
ore
sc
en
ce
(5
33
-61
0)
5 10 15 20 25 30 35 40 45
AdV
Amplification Curves
8.908
8.108
7.308
6.508
5.708
4.908
4.108
3.308
2.508
1.708
0.908
0.108
Cycles
Flu
ore
sc
en
ce
(4
65
-51
0)
5 10 15 20 25 30 35 40 45
InfA
Amplification Curves
26.793
24.293
21.793
19.293
16.793
14.293
11.793
9.293
6.793
4.293
1.793
Cycles
Flu
ore
sc
en
ce
(5
33
-58
0)
5 10 15 20 25 30 35 40 45
InfB
Amplification Curves
Parasite Panel (Serum)
Gastro Panel (Stool) Flu Panel (Swab)
High throughput and scalabilityPrevention of contamination
Three runs (each with 48 positive spiked plasma samples and 48 negative samples arranged in a
checkerboard pattern) were extracted on the MagNA Pure 96 System and then analyzed on the
LightCycler® 480 System. No cross-contamination was found.
Highly reproducible results qPCR results from MagNA Pure 96 extracted samples are highly reproducible (CV <2%) for
intra-run and inter-run, and show reagents' lot-to-lot consistency.
Experiment details
Kit: MagNA Pure 96 DNA and Viral NA
LV Kit
Protocol: Large Volume Protocol (500µL)
Sample: EDTA Plasma
Pathogen: Parvo B19 at 5 x 10^7
copies/mL (=10^6 above detection limit)
0,616
0,566
0,516
0,466
0,416
0,366
0,316
0,266
0,216
0,166
0,116
0,066
0,016
-0,034 10 20 30 40
Flu
ore
sc
en
ce
(4
83
-64
0)
Cycles
Amplification Curves
Negative Positive
2 3 4 5 6 7 8 9 10 11 12 13
A
B
C
D
E
F
G
H
Experiment details
Kit: MagNA Pure 96 DNA and Viral NA SV
Kit
Protocol: Viral NA Universal SV
Sample: EDTA Plasma
Intra-assay – single run, 8 replicates
Inter-assay – 3 runs by different operators
on different instruments in different labs
Lot-to lot – 6 different lots32
30
28
26
24
22
20
181x106 1x105 1x104 1x103
CP
Precision - Hepatitis A Virus
Intra-assay
Inter-assay
Lot-to-lot
30
28
26
24
22
20
18
161x106 1x105 1x104 1x103
CP
Precision - Parvo B19 Virus
Intra-assay
Inter-assay
Lot-to-lot
Growing with your needsScale with ease
Go anywhere with scalability for your lab’s growth. The MagNA Pure 96 Instrument is significantly
faster than two other competitor brands. When considering daily, monthly and yearly capacity needs,
the MagNA Pure 96 Instrument provides increased ability to meet growth and testing fluctuations.1,2
Beyond extraction
Unlock more of the answers you need with the new
4 mL volume protocol for circulating cell-free nucleic
acids (cfNA) on the MagNA Pure 96 System by
preferentially isolating smaller size fragments. Adapt
to fluctuating demand with the only platform to have
the scalability of up to 48 cfNA extractions in one run
and rely on pre-optimized cfNA protocols to address
specific eluate needs for a broad range of downstream
genomic applications, including real-time PCR, arrays,
and next-generation sequencing.
Roche
Competitor Q
Competitor B
0 100 200 300 400
Buccal Swab
Minutes
Roche
Competitor Q
Competitor B
0 100 200 300 400
Whole Blood
Minutes
1000
800
600
400
200
100
0
Testi
ng
Ca
pa
cit
y (
In S
am
ple
s)
384
96
10k
8k
6k
4k
2k
1k
0
7,680
1,920
100k
80k
60k
40k
20k
10k
0
92,160
23,040
Daily Monthly Yearly
(1) Source: Extraction Instrument Comparison; Clinical Lab Consulting, 2016.
(2) Assumes 8 work hour day and whole blood extraction protocol
Experiment details
Kit: MagNA Pure 96 DNA and Viral NA LV Kit
Pretreatment: MagNA Pure cfNA Buffer Set
Protocol: cfNA ds 4000
Sample: EDTA Plasma
Spike in: DNA Ladder (0.05, 0.15, 0.3, 0,5,
0.766, 1, 1.5, 2, 3, 5, 10kb)
Intra-assay – single run, 8 replicates
Inter-assay – 3 runs by different operators
on different instruments in different labs
Lot-to lot – 6 different lots
(FU)
120
100
80
60
40
20
0
-20 35 100 200 300 400 600 2000 10380
Flu
ore
sc
en
ce
Un
its
Fragment Size (base pairs)
Plasma only
Plasma with MagNA Pure cfNA Buffer Set
Bioanalyzer data demonstrates a shift in
extraction size capture with the MagNA Pure
96 DNA and Viral NA LV Kit (dark blue) and
when MagNA Pure cfNA Buffer Set is used
to enhance small fragment isolation (light
blue). Enhance your studies in the genomic
world with new protocols and solutions now
offered on the MagNA Pure 96 System.
Roche Competitor Q
The MagNA Pure 96 System family ensures purity, reproducibility, and
laboratory efficiency obtained only by automated bead-based extraction.
Whether your lab extracts just a few samples or thousands in one day, the
MagNA Pure family has a fully automated extraction to meet your scalable needs.
From low throughput days
• Simplified sample preparation for dramatic reduction
of handling errors
• Preloaded protocols for a broad range of sample types
• Pre-filled and barcoded reagent kits
• Intuitive software and guidance
To high demand times
Expand throughput and automation with Roche
Liquid Handling which combines primary sample
handling and PCR setup in one pipetting instrument.
MagNA Pure 24 System
1-24 Samples | Not available for sale
Roche Liquid Handling
Sample Handling and PCR Setup
Coming in
2017
Start here.
MagNA Pure 96 SystemTechnical Specifications
General
Configuration Benchtop standalone instrument with separate control unit
Number of samples 1 to 96 reactions per run
Sample input volume 50 µL to 1 mL
Up to 4 mL for Plasma sample
Elution volume 50 to 200 µL
Run time Approximately 50 to 90 minutes depending on protocol
Setup time Approximately 5 minutes
Regulatory label For in vitro diagnostic use. Compliant with IVD directive 98/79/EC
Software and Connectivity
Traceability 21CFR part 11 (subsection B), Audit trail, Process monitoring, User guidance
Data export *.xml, LightCycler® sample input file in csv format (*.txt)
Interfaces USB, LAN 10/100/1000 Base T, LAN 10/100 Base
ConnectivityLIMS (e.g., via HL7 transfer protocol), Bidirectional file sharing, remote
Roche Service with Axeda
Hardware
Dimensions W x D x H: 136 x 81.5 x 100 cm
Weight 235 kg
Process parallelization and speed Two robotic heads: (1) Reagent head pipetting arm with four individually
controlled fluid channels to transfer reagents and (2) 96-nozzle process
head to transfer and process samples in parallel.
Contamination control Drop catcher, UV Lamp, dual robotic arm engineering
Kits and Applications
Reagent design Pre-filled, ready-to-use
Unopened kit storage +15 to +25°C
Kit re-use Up to 28 days after first use
Isolation principle Magnetic glass particle technology
Nucleic acids DNA, tNA, viral NA, total RNA, cell-free NA
Supported sample typesWhole blood, plasma, serum, fresh-frozen tissue, FFPE tissue, cultured cells,
urine, swabs, sputum, CSF, BAL, stool
Protocols >30
Go Anywhere.
All MagNA Pure 96 Kits, Consumables, and Accessories are for in vitro diagnostic unless otherwise noted.
* For life science research only. Not for use in diagnostic procedures.
† For general laboratory use.
MAGNA PURE and LIGHTCYCLER are registered trademarks of Roche. All other product names and trademarks are the property of their respective owners.
© 2016 Roche Molecular Systems, Inc. All rights reserved.
Published by
Roche Molecular Systems, Inc
4300 Hacienda Drive
Pleasanton, CA 94588 USA
Product Name Catalog # Content
1. Instrument
MagNA Pure 96 Instrument 06 541 089 001Instrument, control unit,
software, accessories
Ordering informationMagNA Pure 96 Instrument, Kits, and Consumables
2. Reagent Kits and Lysis Buffers
MagNA Pure 96 DNA and Viral NA LV Kit 06 374 891 001 Up to 288 isolations
MagNA Pure 96 DNA and Viral NA SV Kit 06 543 588 001 Up to 596 isolations
MagNA Pure 96 Cellular RNA LV Kit* 05 467 535 001 Up to 288 isolations
MagNA Pure 96 System Fluid (Internal) 06 430 112 001 2 containers
MagNA Pure 96 System Fluid (External) 06 640 729 001 1 container
MagNA Pure 96 External Lysis Buffer 06 374 913 001 100 mL
MagNA Pure 96 Bacterial Lysis Buffer 06 374 921 001 20 mL
MagNA Pure 96 DNA Tissue Lysis Buffer 06 640 702 001 200 mL
MagNA Pure cfNA Buffer Set NEW † 07 794 398 001 Up to 96 isolations
3. Consumables
MagNA Pure 96 Processing Cartridge 06 241 603 001 36
MagNA Pure 96 Output Plate 06 241 611 001 60
MagNA Pure Filter Tip 1000µl 06 241 620 001 8x5
MagNA Pure Sealing Foil† 06 241 638 001 100
MagNA Pure 96 Internal Control Tube 06 374 905 001 150 (15x10)
For more information or general inquiries, or to obtain updated protocols,
please contact your local Roche representative or visit magnapure96.com
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